[go: up one dir, main page]

BR0014468A - Processo de sequenciamento de adn - Google Patents

Processo de sequenciamento de adn

Info

Publication number
BR0014468A
BR0014468A BR0014468-1A BR0014468A BR0014468A BR 0014468 A BR0014468 A BR 0014468A BR 0014468 A BR0014468 A BR 0014468A BR 0014468 A BR0014468 A BR 0014468A
Authority
BR
Brazil
Prior art keywords
dna sequencing
sequencing process
polynucleotide
enzyme
detection
Prior art date
Application number
BR0014468-1A
Other languages
English (en)
Inventor
Daniel Henry Densham
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of BR0014468A publication Critical patent/BR0014468A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

"PROCESSO DE SEQuENCIAMENTO DE ADN". Um processo para determinação da seq³ência de um polinucleotídeo, o processo se baseando na detecção de uma mudança conformacional em uma enzima que interage com e processa ao longo de um polinucleotídeo. A detecção de uma mudança conformacional pode ser realizada por medição de mudanças em um fluoróforo ligado à enzima.
BR0014468-1A 1999-10-06 2000-10-06 Processo de sequenciamento de adn BR0014468A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9923644.0A GB9923644D0 (en) 1999-10-06 1999-10-06 DNA sequencing
PCT/GB2000/003860 WO2001025480A2 (en) 1999-10-06 2000-10-06 Dna sequencing method

Publications (1)

Publication Number Publication Date
BR0014468A true BR0014468A (pt) 2002-08-20

Family

ID=10862234

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014468-1A BR0014468A (pt) 1999-10-06 2000-10-06 Processo de sequenciamento de adn

Country Status (20)

Country Link
US (4) US20050214849A1 (pt)
EP (1) EP1226271B1 (pt)
JP (2) JP5030249B2 (pt)
KR (1) KR100809171B1 (pt)
CN (1) CN1159457C (pt)
AT (1) ATE388242T1 (pt)
AU (1) AU769102B2 (pt)
BR (1) BR0014468A (pt)
CA (1) CA2386115C (pt)
DE (1) DE60038244T2 (pt)
DK (1) DK1226271T3 (pt)
EA (1) EA006702B1 (pt)
ES (1) ES2302497T3 (pt)
GB (1) GB9923644D0 (pt)
IL (2) IL148678A0 (pt)
IS (1) IS6333A (pt)
MX (1) MXPA02003534A (pt)
NZ (1) NZ517775A (pt)
PT (1) PT1226271E (pt)
WO (1) WO2001025480A2 (pt)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1152140C (zh) * 1997-07-28 2004-06-02 医疗生物系统有限公司 核酸序列分析
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB9907812D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
EP1681357A3 (en) 1999-05-19 2006-07-26 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
DE60131194T2 (de) 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire Sequenzbestimmung in echtzeit
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
GB0112238D0 (en) * 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US6902921B2 (en) 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US6956114B2 (en) 2001-10-30 2005-10-18 '454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
SE0201655D0 (sv) * 2002-05-31 2002-05-31 Amersham Biosciences Ab A method of electrophoresis
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
CA2513535C (en) 2003-01-29 2012-06-12 454 Corporation Bead emulsion nucleic acid amplification
EP1644524A1 (en) * 2003-07-15 2006-04-12 Daniel Henry Densham Measurement of a polynucleotide amplification reaction
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
CA2557177A1 (en) 2004-02-19 2005-09-01 Stephen Quake Methods and kits for analyzing polynucleotide sequences
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7635562B2 (en) 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
JP2008513782A (ja) 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 分子解析のための装置及び方法
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
EP3722409A1 (en) * 2006-03-31 2020-10-14 Illumina, Inc. Systems and devices for sequence by synthesis analysis
GB0721340D0 (en) * 2007-10-30 2007-12-12 Isis Innovation Polymerase-based single-molecule DNA sequencing
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US9182406B2 (en) 2008-08-04 2015-11-10 Biodesy, Inc. Nonlinear optical detection of molecules comprising an unnatural amino acid possessing a hyperpolarizability
US20110294116A1 (en) * 2008-12-11 2011-12-01 The Regents of the Unicersity of California Methods and systems for direct sequencing of single dna molecules
US8911972B2 (en) 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US9428789B2 (en) 2011-03-21 2016-08-30 Biodesy, Inc. Classification of kinase inhibitors using nonlinear optical techniques
DE102013202721A1 (de) * 2013-02-20 2014-08-21 Siemens Aktiengesellschaft Sequenziervorrichtung zum Sequenzieren mindestens eines Nukleinsäureeinzelstrangs und Verfahren zum Sequenzieren mindestens eines Nukleinsäureeinzelstrangs
GB201306444D0 (en) * 2013-04-09 2013-05-22 Base4 Innovation Ltd Single nucleotide detection method
US10605766B2 (en) 2014-07-15 2020-03-31 Illumina, Inc. Biochemically activated electronic device
WO2016106286A1 (en) 2014-12-23 2016-06-30 Biodesy, Inc. Attachment of proteins to interfaces for use in nonlinear optical detection
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
AU2017258619B2 (en) 2016-04-29 2020-05-14 Pacific Biosciences Of California, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
CN109844136A (zh) 2016-08-15 2019-06-04 欧姆尼奥姆股份有限公司 测序核酸的方法和系统
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
US11248254B2 (en) 2016-12-30 2022-02-15 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
US10975427B2 (en) 2017-01-20 2021-04-13 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
CA3067420A1 (en) * 2017-09-15 2019-03-21 Illumina, Inc. Sequence-detection system
EP3697932A1 (en) 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
US10344328B2 (en) 2017-11-17 2019-07-09 Ultima Genomics, Inc. Methods for biological sample processing and analysis
US11499962B2 (en) 2017-11-17 2022-11-15 Ultima Genomics, Inc. Methods and systems for analyte detection and analysis
US10512911B1 (en) * 2018-12-07 2019-12-24 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
US12239980B2 (en) 2018-12-07 2025-03-04 Ultima Genomics, Inc. Implementing barriers for controlled environments during sample processing and detection
US11118223B2 (en) 2019-03-14 2021-09-14 Ultima Genomics, Inc. Methods, devices, and systems for analyte detection and analysis
US12060148B2 (en) 2022-08-16 2024-08-13 Honeywell International Inc. Ground resonance detection and warning system and method
CN116287109A (zh) * 2022-09-29 2023-06-23 清华大学 一种测量单分子rna力谱的方法及其应用

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE462454B (sv) 1988-11-10 1990-06-25 Pharmacia Ab Maetyta foer anvaendning i biosensorer
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5583026A (en) * 1994-08-31 1996-12-10 Cornell Research Foundation, Inc. Process for reconstituting the polymerase III* and other subassemblies of E. coli DNA polymerase III holoenzyme from peptide subunits
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5360714A (en) * 1992-08-28 1994-11-01 Fox Chase Cancer Center Hepadnavirus polymerase gene product having RNA-dependent DNA priming and reverse transcriptase activities and methods of measuring the activities thereof
DE69430909T2 (de) 1993-03-19 2003-02-27 Sequenom, Inc. Dns-sequenzbestimmung durch massenspektrometrie auf dem weg des abbaus mit exonuklease
WO1995006138A1 (en) * 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5747247A (en) * 1994-07-25 1998-05-05 The Regents Of The University Of California Spectroscopic helicase assay
US5801042A (en) * 1994-08-18 1998-09-01 The Trustees Of Columbia University In The City Of New York Unique associated Kaposi's sarcoma virus sequences and uses thereof
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US6331392B1 (en) * 1997-03-05 2001-12-18 Anadys Pharmaceutical, Inc. Screen employing fluorescence anisotropy to identify compounds with affinity for nucleic acids
US6159687A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods for generating recombined polynucleotides
CN1152140C (zh) 1997-07-28 2004-06-02 医疗生物系统有限公司 核酸序列分析
US7875440B2 (en) * 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
JP2002522780A (ja) 1998-08-13 2002-07-23 ユー．エス．ゲノミクス ポリマーの光学的な特性決定
US6210896B1 (en) * 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US7270951B1 (en) * 1999-03-10 2007-09-18 Asm Scientific, Inc. Method for direct nucleic acid sequencing
GB9907812D0 (en) * 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6335420B1 (en) 1999-11-22 2002-01-01 Ibc Advanced Technologies, Inc. Polyamide ligand-containing polymeric resins and methods of using the same for removing, separating and/or concentrating desired metal ions from solutions
US6908736B1 (en) * 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
GB0413082D0 (en) 2004-06-11 2004-07-14 Medical Biosystems Ltd Method
US8262900B2 (en) * 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20100301398A1 (en) * 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) * 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8574835B2 (en) * 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8673627B2 (en) * 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions

Also Published As

Publication number Publication date
AU7546700A (en) 2001-05-10
EA200200428A1 (ru) 2002-10-31
EP1226271A2 (en) 2002-07-31
KR100809171B1 (ko) 2008-02-29
CN1159457C (zh) 2004-07-28
EP1226271B1 (en) 2008-03-05
ATE388242T1 (de) 2008-03-15
DE60038244D1 (de) 2008-04-17
PT1226271E (pt) 2008-05-23
US7939264B1 (en) 2011-05-10
ES2302497T3 (es) 2008-07-16
IL148678A0 (en) 2002-09-12
AU769102B2 (en) 2004-01-15
CN1391615A (zh) 2003-01-15
US20110177520A1 (en) 2011-07-21
KR20020059444A (ko) 2002-07-12
US20120214164A1 (en) 2012-08-23
JP5030249B2 (ja) 2012-09-19
EA006702B1 (ru) 2006-02-24
US20050214849A1 (en) 2005-09-29
DE60038244T2 (de) 2009-04-23
HK1045857A1 (en) 2002-12-13
IS6333A (is) 2002-04-05
CA2386115A1 (en) 2001-04-12
JP2012196201A (ja) 2012-10-18
CA2386115C (en) 2010-06-15
WO2001025480A2 (en) 2001-04-12
GB9923644D0 (en) 1999-12-08
NZ517775A (en) 2004-01-30
DK1226271T3 (da) 2008-07-07
JP2003511043A (ja) 2003-03-25
MXPA02003534A (es) 2004-09-10
WO2001025480A3 (en) 2002-05-16
IL148678A (en) 2008-12-29

Similar Documents

Publication Publication Date Title
BR0014468A (pt) Processo de sequenciamento de adn
ATE419392T1 (de) Verfahren zum nachweis von alkyliertem cytosin in dna
ATE383442T1 (de) Verfahren zur sequenzierung von polynukleotiden
ATE394677T1 (de) Fret proben und verfahren zur erkennung aufeinander einwirkeneden moleküle
DK1157264T3 (da) Biokemiske oprensningsindretninger med immobiliserede opfangningsprober og anvendelser deraf
DE60232323D1 (de) Verbessertes verfahren zum nachweis von atp
ATE368125T1 (de) Verfahren zur erkennung von einzel-nukleotid- polymorphismen
DE60136567D1 (de) Polynukleotid-proben zum nachweis und zur quantifizierung von candida spezies
TW200720441A (en) New labelling strategies for the sensitive detection of analytes
GB2346695A (en) Fluorometric method for monitoring and detecting nucleic acid amplification
ATE264923T1 (de) Verfahren und system zur erkennung von oligonukleotiden in einer probe
DE69833660D1 (de) Nukleinsäuresonden zur detektion von candida-spezies
DE69604051D1 (de) Verfahren zum sensitiven nachweis von reverser transcriptase
DE60130970D1 (de) Reagenziensatz zur detektion von mikroorganismen, vorrichtung zur quantifizierungvon mikroorganismen und verfahren zur quantifizierung von mikroorganismen
ATE298786T1 (de) In situ-hybridisierungs-anordnung zum spezifischen nachweis von mikroorganismen
DE50013309D1 (de) Verfahren und nukleinsäuren zum nachweis von brauereirelevanten mikroorganismen
WO2004000995A3 (en) Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms
DE60043618D1 (de) Verfahren zum nachweis einer reaktion zur molekularen erkennung
DE50214861D1 (de) Verfahren zum erfassen von nukleinsäuremolekülen mittels mindestens einer einheit zum immobilisieren von nukleinsäuremolekülen und biosensor zum erfassen von nukleinsäuremolekülen
DE50208262D1 (de) Verfahren zur bestimmung von flüchtigen substanzen in lösung
DE50207850D1 (de) Biosensor und verfahren zum erfassen von nukleinsäuren mittels mindestens zwei einheiten zum immobilisieren von nukleinsäuren
FR2805545B1 (fr) Procede electrochimique de detection d'acides nucleiques
ATE466961T1 (de) Verfahren zum nachweis von norwalk-ähnlichem virus (gi)
ATE439587T1 (de) Verwendung eines biologischen sensors zum nachweis von antibiotika
DE60323834D1 (de) Von bacillus cereus produzierte cereulid-synthase, diese codierendes gen und verfahren zum nachweis von cereulid

Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O, 11, 13 E 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.