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AU5030900A - Construction of incremental truncation libraries - Google Patents

Construction of incremental truncation libraries

Info

Publication number
AU5030900A
AU5030900A AU50309/00A AU5030900A AU5030900A AU 5030900 A AU5030900 A AU 5030900A AU 50309/00 A AU50309/00 A AU 50309/00A AU 5030900 A AU5030900 A AU 5030900A AU 5030900 A AU5030900 A AU 5030900A
Authority
AU
Australia
Prior art keywords
construction
incremental truncation
truncation libraries
libraries
incremental
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU50309/00A
Inventor
Stephen J Benkovic
Stefan A. Lutz
Andrew E. Nixon
Marc Ostermeier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Penn State Research Foundation
Original Assignee
Penn State Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Penn State Research Foundation filed Critical Penn State Research Foundation
Publication of AU5030900A publication Critical patent/AU5030900A/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU50309/00A 1999-05-21 2000-05-19 Construction of incremental truncation libraries Abandoned AU5030900A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13542999P 1999-05-21 1999-05-21
US60135429 1999-05-21
US17252599P 1999-12-17 1999-12-17
US60172525 1999-12-17
PCT/US2000/013813 WO2000072013A1 (en) 1999-05-21 2000-05-19 Construction of incremental truncation libraries

Publications (1)

Publication Number Publication Date
AU5030900A true AU5030900A (en) 2000-12-12

Family

ID=26833315

Family Applications (1)

Application Number Title Priority Date Filing Date
AU50309/00A Abandoned AU5030900A (en) 1999-05-21 2000-05-19 Construction of incremental truncation libraries

Country Status (2)

Country Link
AU (1) AU5030900A (en)
WO (1) WO2000072013A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7153655B2 (en) 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US7332308B1 (en) * 1999-05-21 2008-02-19 The Penn State Research Foundation Incrementally truncated nucleic acids and methods of making same
PT1341909E (en) * 2000-12-12 2005-10-31 Alligator Bioscience Ab METHOD FOR THE IN VITRO MOLECULAR EVOLUTION OF PROTEIN FUNCTION
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
CA2485506C (en) 2002-05-17 2012-02-28 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
EP1842915A1 (en) * 2006-04-04 2007-10-10 Libragen Method of in vitro polynucleotide sequences shuffling by recursive circular DNA molecules fragmentation and ligation

Also Published As

Publication number Publication date
WO2000072013A1 (en) 2000-11-30

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase