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ATE424467T1 - Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins - Google Patents

Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins

Info

Publication number
ATE424467T1
ATE424467T1 AT99933168T AT99933168T ATE424467T1 AT E424467 T1 ATE424467 T1 AT E424467T1 AT 99933168 T AT99933168 T AT 99933168T AT 99933168 T AT99933168 T AT 99933168T AT E424467 T1 ATE424467 T1 AT E424467T1
Authority
AT
Austria
Prior art keywords
cell
free protein
protein synthesis
producing
synthesis reaction
Prior art date
Application number
AT99933168T
Other languages
English (en)
Inventor
Yaeta Endo
Shigemichi Nishikawa
Original Assignee
Cellfree Sciences Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP11130393A external-priority patent/JP2000316594A/ja
Priority claimed from JP11130395A external-priority patent/JP2000316595A/ja
Priority claimed from JP11151599A external-priority patent/JP2000333673A/ja
Application filed by Cellfree Sciences Co Ltd filed Critical Cellfree Sciences Co Ltd
Application granted granted Critical
Publication of ATE424467T1 publication Critical patent/ATE424467T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT99933168T 1999-05-11 1999-07-29 Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins ATE424467T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP11130393A JP2000316594A (ja) 1999-05-11 1999-05-11 無細胞タンパク質合成用細胞抽出物含有製剤、及び無細胞タンパク質合成反応用キット
JP11130395A JP2000316595A (ja) 1999-05-11 1999-05-11 無細胞タンパク質合成手段
JP11151599A JP2000333673A (ja) 1999-05-31 1999-05-31 連続無細胞タンパク質合成手段

Publications (1)

Publication Number Publication Date
ATE424467T1 true ATE424467T1 (de) 2009-03-15

Family

ID=27316108

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99933168T ATE424467T1 (de) 1999-05-11 1999-07-29 Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins

Country Status (9)

Country Link
US (2) US6905843B1 (de)
EP (1) EP1176210B1 (de)
KR (1) KR100546009B1 (de)
AT (1) ATE424467T1 (de)
AU (1) AU765632B2 (de)
CA (1) CA2373057A1 (de)
DE (1) DE69940525D1 (de)
EA (1) EA006162B1 (de)
WO (1) WO2000068412A1 (de)

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CA2373057A1 (en) * 1999-05-11 2000-11-16 Wakenyaku Co., Ltd. Preparation containing cell extracts for cell-free protein synthesis and means for synthesizing protein using the preparation
DE10011209A1 (de) * 2000-03-08 2001-09-13 Roche Diagnostics Gmbh Matrix-Reaktor und Verfahren zur Herstellung von Produkten in diesem Reaktor
WO2002008443A1 (fr) * 2000-07-21 2002-01-31 National Institute Of Advanced Industrial Science And Technology Dispositif destine a marquer une proteine au moyen d'un systeme de synthese de proteine acellulaire d'embryon de ble
US6869774B2 (en) 2000-08-29 2005-03-22 Yaeta Endo Methods of synthesizing cell-free protein
JP4838957B2 (ja) 2001-05-02 2011-12-14 独立行政法人理化学研究所 無細胞タンパク質合成系を用いたタンパク質の製造方法
DE10137792A1 (de) 2001-08-06 2003-02-27 Erdmann Volker Verfahren zur Genexpression
US20040248140A1 (en) * 2001-12-27 2004-12-09 Yaeta Endo Nucleotidic sequences having activity of controlling translation efficiency and utilization thereof
EP1477566B1 (de) * 2002-01-31 2007-09-26 CellFree Sciences Co., Ltd. Keimextrakt zur zellfreien proteinsynthese und verfahren zu dessen herstellung
CA2477440A1 (en) 2002-02-28 2003-09-04 Yaeta Endo Reaction solution for cell-free protein synthesis, method of preparing the same and protein synthesis method using the same
CA2485827A1 (en) * 2002-05-14 2003-11-20 Cellfree Sciences Co., Ltd. Lyophilized preparation for synthesis of cell-free protein
EP1507003B1 (de) 2002-05-22 2011-09-21 Riken Verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung eines thioredoxinfusionsprotein-expressionsvektors
JP4441170B2 (ja) 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
JP2006129702A (ja) * 2002-12-05 2006-05-25 Zoegene Corp タンパク質製造方法
JP2004208640A (ja) * 2003-01-07 2004-07-29 Rengo Co Ltd 無細胞系タンパク質合成用酵母抽出液およびその調製方法、ならびにそれを用いた無細胞系タンパク質合成方法
WO2005015212A1 (en) * 2003-08-07 2005-02-17 Cellfree Sciences Co., Ltd. Reagent for producing a protein chip
US20060188947A1 (en) * 2003-08-07 2006-08-24 Yaeta Endo Reagent for producing a protein chip
US20060233789A1 (en) * 2004-09-24 2006-10-19 Cellfree Science Co., Ltd. Process for producing antigenic substance
WO2006039622A2 (en) * 2004-10-01 2006-04-13 Invitrogen Corporation Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
JP2007199047A (ja) 2005-12-28 2007-08-09 Cellfree Sciences Co Ltd ビオチン化タンパク質の調製方法及び該タンパク質を用いた検出方法
US20070207520A1 (en) * 2006-03-02 2007-09-06 Ryo Morishita Method for cell-free synthesizing protein using wheat embryo extract under low temperature condition
US20100310548A1 (en) * 2007-10-15 2010-12-09 Biogen Idec Ma Inc. Methods of Manufacturing a Biologic Using a Stable Storage Intermediate
DK2213747T3 (da) 2007-11-05 2021-08-09 Riken Fremgangsmåde til fremstilling af membranprotein
US8522515B2 (en) * 2009-01-26 2013-09-03 R.J. Reynolds Tobacco Company Method and apparatus for customizing cigarette packages
AU2010234206B2 (en) * 2009-04-06 2016-10-27 Crearene Ag Hemodialysis and peritoneal dialysis solutions comprising one or more creatine compounds
WO2011008867A1 (en) * 2009-07-15 2011-01-20 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of active reprogramming transcription factors
KR101161786B1 (ko) * 2009-12-18 2012-07-03 (주)바이오쉴드 번역효율 증가제로서 계면활성제를 포함하는 무세포 단백질 합성계에서 펩타이드를 합성하는 방법 및 이를 위한 조성물
US10774304B2 (en) 2011-03-08 2020-09-15 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
US9982227B2 (en) 2011-03-08 2018-05-29 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
US12227734B2 (en) 2011-03-08 2025-02-18 University Of Maryland, Baltimore County Microscale bioprocessing system and method for protein manufacturing from human blood
US20120301904A1 (en) 2011-04-26 2012-11-29 Prosetta Antiviral, Inc Multiprotein assemblies
WO2013080811A1 (ja) 2011-11-28 2013-06-06 国立大学法人名古屋大学 リンパ球性漏斗下垂体後葉炎のバイオマーカー及びその用途
WO2015085209A1 (en) * 2013-12-06 2015-06-11 President And Fellows Of Harvard College Paper-based synthetic gene networks
US9078427B1 (en) 2014-08-29 2015-07-14 Pioneer Hi Bred International Inc Method of storing plant embryos
WO2016032629A1 (en) 2014-08-29 2016-03-03 Pioneer Hi-Bred International, Inc. Methods and devices involving oil matrices
WO2016112258A1 (en) 2015-01-08 2016-07-14 Sutro Biopharma, Inc. Formulations for drying bacterial cell extracts
WO2017022696A1 (ja) 2015-07-31 2017-02-09 国立研究開発法人理化学研究所 膜タンパク質の製造方法およびその利用
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11673921B2 (en) 2017-11-10 2023-06-13 Northwestern University Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
WO2020010356A1 (en) 2018-07-06 2020-01-09 Northwestern University Ribosome variants for sequence defined polymer synthesis
WO2020072127A2 (en) 2018-08-03 2020-04-09 Northwestern University On demand, portable, cell-free molecular sensing platform
EP3931341A4 (de) 2019-02-25 2023-03-29 Northwestern University Zellfreie, aus clostridien gewonnene proteinsyntheseplattformen
WO2023010053A1 (en) * 2021-07-28 2023-02-02 Northwestern University A low-cost, thermostable, lyophilized, cell-free protein synthesis platform
JPWO2025063209A1 (de) * 2023-09-18 2025-03-27
KR102873056B1 (ko) * 2023-11-15 2025-10-23 대한민국(방위사업청장) 무세포 단백질 합성 반응액의 안정한 보관을 위한 동결건조 제형 및 이의 제조방법
CN118022387B (zh) * 2024-04-11 2024-07-26 福建龙生生物科技有限公司 一种融合蛋白制备装置

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WO1993007287A1 (en) * 1991-10-11 1993-04-15 Promega Corporation Coupled transcription and translation in eukaryotic cell-free extract
JPH07147992A (ja) * 1993-11-30 1995-06-13 Kobe Steel Ltd 無細胞抽出液を利用したポリペプチドの合成方法
JP3255784B2 (ja) * 1994-01-26 2002-02-12 弥重太 遠藤 蛋白質の合成方法
KR0131166B1 (ko) * 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법
JPH1080295A (ja) * 1996-07-15 1998-03-31 Nippon Flour Mills Co Ltd 無細胞タンパク合成系によるタンパクの合成方法及び装置
ATE357513T1 (de) * 1996-07-16 2007-04-15 Qiagen North American Holdings Verfahren zur herstellung komplexer multienzymatischer lagerstabiler reaktionsgemische und deren verwendung
CA2373057A1 (en) * 1999-05-11 2000-11-16 Wakenyaku Co., Ltd. Preparation containing cell extracts for cell-free protein synthesis and means for synthesizing protein using the preparation

Also Published As

Publication number Publication date
EP1176210A1 (de) 2002-01-30
AU4930199A (en) 2000-11-21
CA2373057A1 (en) 2000-11-16
US7235382B2 (en) 2007-06-26
KR100546009B1 (ko) 2006-01-25
KR20010112953A (ko) 2001-12-22
US20050186655A1 (en) 2005-08-25
US6905843B1 (en) 2005-06-14
DE69940525D1 (de) 2009-04-16
EA200101189A1 (ru) 2002-04-25
WO2000068412A1 (en) 2000-11-16
EP1176210B1 (de) 2009-03-04
AU765632B2 (en) 2003-09-25
EA006162B1 (ru) 2005-10-27
EP1176210A4 (de) 2004-09-08

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