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ATE278035T1 - Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien - Google Patents

Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien

Info

Publication number
ATE278035T1
ATE278035T1 AT00928615T AT00928615T ATE278035T1 AT E278035 T1 ATE278035 T1 AT E278035T1 AT 00928615 T AT00928615 T AT 00928615T AT 00928615 T AT00928615 T AT 00928615T AT E278035 T1 ATE278035 T1 AT E278035T1
Authority
AT
Austria
Prior art keywords
nucleic acids
sample
fast
cell lysis
lysis reagents
Prior art date
Application number
AT00928615T
Other languages
English (en)
Inventor
Robert T Belly
Jianbo Sun
Original Assignee
Ortho Clinical Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ortho Clinical Diagnostics Inc filed Critical Ortho Clinical Diagnostics Inc
Application granted granted Critical
Publication of ATE278035T1 publication Critical patent/ATE278035T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
AT00928615T 1999-05-04 2000-05-01 Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien ATE278035T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13244399P 1999-05-04 1999-05-04
PCT/US2000/011651 WO2000066783A2 (en) 1999-05-04 2000-05-01 Rapid and efficient capture of dna from sample without using cell lysing reagent

Publications (1)

Publication Number Publication Date
ATE278035T1 true ATE278035T1 (de) 2004-10-15

Family

ID=22454077

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00928615T ATE278035T1 (de) 1999-05-04 2000-05-01 Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien

Country Status (11)

Country Link
US (2) US7615346B2 (de)
EP (1) EP1206571B1 (de)
JP (2) JP4643023B2 (de)
AT (1) ATE278035T1 (de)
AU (1) AU4682400A (de)
CA (1) CA2370122C (de)
DE (1) DE60014399T2 (de)
DK (1) DK1206571T3 (de)
ES (1) ES2228520T3 (de)
PT (1) PT1206571E (de)
WO (1) WO2000066783A2 (de)

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US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
JP5415253B2 (ja) 2006-03-24 2014-02-12 ハンディラブ・インコーポレーテッド 微小流体サンプルを処理するための一体化システム及びその使用方法
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
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US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8206929B2 (en) 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP2013004258A (ja) * 2011-06-15 2013-01-07 Gigaphoton Inc 極端紫外光生成装置及び極端紫外光の生成方法
BR112013026451B1 (pt) 2011-04-15 2021-02-09 Becton, Dickinson And Company sistema e método para realizar ensaios de diagnóstico molecular em várias amostras em paralelo e simultaneamente amplificação em tempo real em pluralidade de câmaras de reação de amplificação
US9885080B2 (en) * 2011-05-31 2018-02-06 Berry Genomics Co., Ltd. Kit, a device and a method for detecting copy number of fetal chromosomes or tumor cell chromosomes
CN113388605A (zh) * 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
WO2013067202A1 (en) 2011-11-04 2013-05-10 Handylab, Inc. Polynucleotide sample preparation device
CN105378108A (zh) * 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法

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Also Published As

Publication number Publication date
JP2011015691A (ja) 2011-01-27
US20070243542A1 (en) 2007-10-18
AU4682400A (en) 2000-11-17
CA2370122A1 (en) 2000-11-09
PT1206571E (pt) 2004-12-31
DK1206571T3 (da) 2004-12-06
EP1206571B1 (de) 2004-09-29
WO2000066783A3 (en) 2002-01-03
CA2370122C (en) 2011-04-26
US20090280498A1 (en) 2009-11-12
EP1206571A2 (de) 2002-05-22
JP4643023B2 (ja) 2011-03-02
DE60014399D1 (de) 2004-11-04
WO2000066783A2 (en) 2000-11-09
ES2228520T3 (es) 2005-04-16
US7615346B2 (en) 2009-11-10
DE60014399T2 (de) 2005-12-08
JP2004508002A (ja) 2004-03-18

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