[go: up one dir, main page]

AR132535A1 - Evento de maíz transgénico qy2569-42 y su procedimiento de detección - Google Patents

Evento de maíz transgénico qy2569-42 y su procedimiento de detección

Info

Publication number
AR132535A1
AR132535A1 ARP240101063A ARP240101063A AR132535A1 AR 132535 A1 AR132535 A1 AR 132535A1 AR P240101063 A ARP240101063 A AR P240101063A AR P240101063 A ARP240101063 A AR P240101063A AR 132535 A1 AR132535 A1 AR 132535A1
Authority
AR
Argentina
Prior art keywords
seq
nucleic acid
acid sequence
corn plant
detection procedure
Prior art date
Application number
ARP240101063A
Other languages
English (en)
Inventor
Bo Chen
Huarong Li
Yongchun Wu
Lei Lian
Dehui Ding
Original Assignee
Qingdao Kingagroot Seed Science Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qingdao Kingagroot Seed Science Co Ltd filed Critical Qingdao Kingagroot Seed Science Co Ltd
Publication of AR132535A1 publication Critical patent/AR132535A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684—Zea mays [maize]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8277—Phosphinotricin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01183—Phosphinothricin acetyltransferase (2.3.1.183)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se relaciona con evento de maíz transgénico, QY2569-42, y además se relaciona con una secuencia de ácido nucleico para detectar una planta de maíz QY2569-42 y su procedimiento de detección. La secuencia de ácido nucleico de la planta de maíz comprende SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5, SEQ ID Nº 6, SEQ ID Nº 7, SEQ ID Nº 8, SEQ ID Nº 10, SEQ ID Nº 11, y/o SEQ ID Nº 12, o una secuencia de ácido nucleico complementaria a ella. La planta de maíz QY2569-42 de la presente invención presenta buena resistencia ante los insectos lepidópteranos y buena tolerancia al herbicida de glufosinato sin impactar el rendimiento. Además, el procedimiento de detección puede identificar de forma precisa y rápida si una muestra biológica comprende moléculas de ADN del evento de maíz transgénico QY2569-42.
ARP240101063A 2023-08-28 2024-04-26 Evento de maíz transgénico qy2569-42 y su procedimiento de detección AR132535A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202311093966.XA CN119530421A (zh) 2023-08-28 2023-08-28 转基因玉米事件qy2569-42及其检测方法

Publications (1)

Publication Number Publication Date
AR132535A1 true AR132535A1 (es) 2025-07-16

Family

ID=94705856

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP240101063A AR132535A1 (es) 2023-08-28 2024-04-26 Evento de maíz transgénico qy2569-42 y su procedimiento de detección

Country Status (5)

Country Link
EP (1) EP4541892A1 (es)
CN (1) CN119530421A (es)
AR (1) AR132535A1 (es)
CO (1) CO2025003942A2 (es)
WO (1) WO2025043835A1 (es)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104878091B (zh) * 2015-04-30 2019-05-17 北京大北农科技集团股份有限公司 用于检测玉米植物dbn9978的核酸序列及其检测方法
CN109971880B (zh) * 2019-04-09 2022-11-04 北京大北农生物技术有限公司 用于检测玉米植物dbn9508的核酸序列及其检测方法
CN109868273B (zh) * 2019-04-09 2023-02-03 北京大北农生物技术有限公司 用于检测玉米植物dbn9501的核酸序列及其检测方法
BR102019023319A2 (pt) * 2019-11-06 2021-05-18 Embrapa-Empresa Brasileira De Pesquisa Agropecuaria molécula de ácido nucléico do evento transgênico de milho me240913 expressando a proteína cry1da, célula, planta e semente transgênica, usos das mesmas, produto de planta, método, kit e amplicon para detecção do evento, e métodos para produzir uma planta transgênica e de controle de insetos-pragas lepidópteros
CN116219063A (zh) * 2023-03-13 2023-06-06 北京大北农生物技术有限公司 用于检测玉米植物dbn9235的核酸序列及其检测方法

Also Published As

Publication number Publication date
CO2025003942A2 (es) 2025-07-17
WO2025043835A1 (zh) 2025-03-06
EP4541892A1 (en) 2025-04-23
CN119530421A (zh) 2025-02-28

Similar Documents

Publication Publication Date Title
AR118492A1 (es) Secuencias de ácido nucleico y métodos para detectar planta de maíz dbn9501
Kim et al. Development of a simple and accurate molecular tool for Spodoptera frugiperda species identification using LAMP
UY40149A (es) Secuencias de ácido nucleico para la detección de la planta de soja DBN8205 y método de detección de
AR119616A1 (es) Secuencias de ácido nucleico para detectar planta de soja dbn8002 y sus métodos de detección
AR132127A1 (es) Secuencia de ácido nucleico para la detección de la planta de maíz dbn9235 y método de detección de la misma
AR115872A1 (es) Evento transgénico de maíz mon 95379 y métodos para su detección y usos
Balme-Sinibaldi et al. Improvement of Potato virus Y (PVY) detection and quantitation using PVYN-and PVYO-specific real-time RT-PCR assays
KR102135169B1 (ko) 소나무재선충 검출용 등온증폭 반응 프라이머
AR132130A1 (es) Secuencia de ácido nucleico para detectar la planta de maíz dbn9229 y método de detección de la misma
Sasaya Detection methods for rice viruses by a reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
KR101892085B1 (ko) 벼멸구 검출용 고리매개등온증폭용 프라이머 세트 및 이를 이용한 벼멸구 검출 방법
AR132535A1 (es) Evento de maíz transgénico qy2569-42 y su procedimiento de detección
Przybylska et al. PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis
CN118791586B (zh) 小麦抗病因子TaADR1在条锈病防治中的应用
US9023602B2 (en) Method for detecting cyst nematodes
Przybylska et al. Real-time PCR Assay for Distinguishing Frankliniella Occidentalis and Thrips Palmi Arnika Przybylska, Żaneta Fiedler, Aleksandra Obrępalska-Stęplowska
KR101705577B1 (ko) 멜론 괴저반점 바이러스 저항성 및 이병성 품종 판별용 프라이머 세트 및 이를 이용한 멜론 괴저반점 바이러스 저항성 및 이병성 품종 선별방법
Kolade et al. Molecular diagnostics for plant viruses towards enhanced delivery of disease-free planting materials and germplasm exchange
Predajňa et al. First report of potato virus s infecting tomato in Slovakia.
Singh et al. Deciphering the Pathogenic Behaviour of Phyto‐Pathogens Using Molecular Tools
Aslam et al. Molecular diagnostics of plant viruses, viroids, and phytoplasma: an updated overview
CN107988337A (zh) 一种抗稻瘟水稻鉴定方法和基因的标记方法及其应用
Kashyap et al. Identification and diagnosis of wheat blast
KR20230039866A (ko) 고추에서 발생하는 5종의 곰팡이 진단을 위한 프라이머 및 프로브 세트 및 진단 방법
Du et al. Rapid and Visual Detection of Pyricularia oryzae Using Coupled Recombinase Polymerase Amplification–Lateral Flow Dipstick Assay