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AR121142A1 - Microorganismo que comprende un lyse mutado y método de producción de un l-aminoácido que lo utiliza - Google Patents

Microorganismo que comprende un lyse mutado y método de producción de un l-aminoácido que lo utiliza

Info

Publication number
AR121142A1
AR121142A1 ARP210100191A ARP210100191A AR121142A1 AR 121142 A1 AR121142 A1 AR 121142A1 AR P210100191 A ARP210100191 A AR P210100191A AR P210100191 A ARP210100191 A AR P210100191A AR 121142 A1 AR121142 A1 AR 121142A1
Authority
AR
Argentina
Prior art keywords
lyse
mutated
amino acid
microorganism
production
Prior art date
Application number
ARP210100191A
Other languages
English (en)
Inventor
Jaewon Jang
Jihyun Shim
Sang Min Park
Hyun Won Bae
Hyo Jeong Byun
Yong Uk Shin
Han Hyoung Lee
Boram Lim
Moo Young Jung
Yunjung Choi
Original Assignee
Cj Cheiljedang Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of AR121142A1 publication Critical patent/AR121142A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se provee un microorganismo que comprende un LysE mutado, y un método para producir un L-aminoácido que lo utiliza. El LysE mutado tiene un efecto de mejorar la habilidad de exportar y/o producir un L-aminoácido, en comparación con un LysE salvaje.
ARP210100191A 2020-02-13 2021-01-26 Microorganismo que comprende un lyse mutado y método de producción de un l-aminoácido que lo utiliza AR121142A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020200017559A KR102139806B1 (ko) 2020-02-13 2020-02-13 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법

Publications (1)

Publication Number Publication Date
AR121142A1 true AR121142A1 (es) 2022-04-20

Family

ID=71839381

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP210100191A AR121142A1 (es) 2020-02-13 2021-01-26 Microorganismo que comprende un lyse mutado y método de producción de un l-aminoácido que lo utiliza

Country Status (11)

Country Link
US (1) US12503712B2 (es)
EP (1) EP4105229A4 (es)
JP (1) JP7422237B2 (es)
KR (1) KR102139806B1 (es)
CN (1) CN113544141B (es)
AR (1) AR121142A1 (es)
AU (1) AU2021221374B2 (es)
MX (1) MX2022009929A (es)
MY (1) MY199870A (es)
WO (1) WO2021162459A1 (es)
ZA (1) ZA202207846B (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102139806B1 (ko) * 2020-02-13 2020-07-30 씨제이제일제당 (주) 변이형 LysE를 포함하는 미생물, 및 이를 이용한 L-아미노산 생산 방법
US20230313244A1 (en) 2020-09-03 2023-10-05 Daesang Corporation Corynebacterium glutamicum mutant strain having enhanced l-lysine productivity and method of producing l-lysine using the same
KR102703188B1 (ko) 2020-09-03 2024-09-06 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102616694B1 (ko) * 2020-12-09 2023-12-20 씨제이제일제당 (주) 쉬와넬라 아틀란티카 유래 단백질을 발현하는 미생물, 및 이를 이용한 l-아미노산 생산 방법
KR102617168B1 (ko) * 2020-12-09 2023-12-21 씨제이제일제당 (주) 쉬와넬라 오네이덴시스 유래 단백질을 발현하는 미생물, 및 이를 이용한 l-아미노산 생산 방법
KR102277034B1 (ko) * 2021-01-29 2021-07-13 씨제이제일제당 (주) 신규한 dahp 신타아제 변이체 및 이를 이용한 l-라이신 생산 방법
JP7743531B2 (ja) 2021-03-09 2025-09-24 デサン・コーポレイション Ｌ－リジン生産能が向上したコリネバクテリウム・グルタミカム変異株およびこれを用いたｌ－リジンの生産方法
KR102685495B1 (ko) 2021-03-09 2024-07-16 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR20220126610A (ko) 2021-03-09 2022-09-16 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
BR112023018219A2 (pt) 2021-03-09 2023-11-28 Daesang Corp Variante de corynebacterium glutamicum que tem capacidade de produção de l-lisina aprimorada e método para produzir l-lisina com o uso da mesma
CN113201555A (zh) * 2021-04-01 2021-08-03 云南师范大学 一种含有eGFP标记和潮霉素抗性双元载体的构建方法
US20240218405A1 (en) 2021-04-29 2024-07-04 Cj Cheiljedang Corporation Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine using same
KR20220148694A (ko) 2021-04-29 2022-11-07 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
CA3217309A1 (en) 2021-04-30 2022-11-03 Cj Cheiljedang Corporation Corynebacterium glutamicum variant having improved l-lysine production ability, and method for producing l-lysine by using same
JP7620123B2 (ja) 2021-04-30 2025-01-22 シージェイ チェイルジェダング コーポレイション Ｌ－リシン生産能が向上したコリネバクテリウム・グルタミカム変異株及びそれを用いたｌ－リシン生産方法
KR20220149376A (ko) 2021-04-30 2022-11-08 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102668767B1 (ko) 2021-04-30 2024-05-24 씨제이제일제당 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102682180B1 (ko) 2021-04-30 2024-07-05 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102703209B1 (ko) 2021-10-14 2024-09-05 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102875556B1 (ko) * 2021-10-26 2025-10-23 씨제이제일제당 주식회사 LysE 변이체 및 이를 이용한 L-아르기닌 생산방법
KR102876704B1 (ko) 2021-12-06 2025-10-28 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 글루타미쿰 변이주 및 이를 이용한 l-라이신의 생산 방법
KR102833988B1 (ko) * 2022-03-18 2025-07-11 씨제이제일제당 (주) 거짓쌀도둑거저리 유래 아스파테이트 1-디카복실레이스의 변이체 및 이를 포함하는 미생물
KR102761742B1 (ko) 2022-03-21 2025-02-03 대상 주식회사 코리네박테리움 발현용 신규 프로모터 및 이의 용도
KR20240142275A (ko) 2023-03-20 2024-09-30 대상 주식회사 L-라이신 생산능이 향상된 변이 미생물 및 이를 이용한 l-라이신의 생산 방법
KR20250117544A (ko) * 2024-01-26 2025-08-05 씨제이제일제당 (주) 신규한 리보스 포스페이트 디포스포키나제 변이체 및 이를 이용한 l-아미노산 생산 방법
KR20250139930A (ko) 2024-03-14 2025-09-24 대상 주식회사 L-라이신 생산능이 향상된 코리네박테리움 속 미생물 및 이를 이용한 l-라이신의 생산 방법

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Also Published As

Publication number Publication date
AU2021221374B2 (en) 2023-05-18
BR112022015945A2 (pt) 2022-10-04
ZA202207846B (en) 2023-12-20
KR102139806B1 (ko) 2020-07-30
JP7422237B2 (ja) 2024-01-25
MX2022009929A (es) 2022-09-09
JP2023512499A (ja) 2023-03-27
MY199870A (en) 2023-11-27
US20230072150A1 (en) 2023-03-09
WO2021162459A1 (ko) 2021-08-19
EP4105229A4 (en) 2025-07-16
CN113544141A (zh) 2021-10-22
AU2021221374A1 (en) 2022-07-14
EP4105229A1 (en) 2022-12-21
US12503712B2 (en) 2025-12-23
CN113544141B (zh) 2023-10-31

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