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AR127377A1 - Mutantes de beta vulgaris tolerantes a herbicidas inhibidores de als - Google Patents

Mutantes de beta vulgaris tolerantes a herbicidas inhibidores de als

Info

Publication number
AR127377A1
AR127377A1 ARP220102807A ARP220102807A AR127377A1 AR 127377 A1 AR127377 A1 AR 127377A1 AR P220102807 A ARP220102807 A AR P220102807A AR P220102807 A ARP220102807 A AR P220102807A AR 127377 A1 AR127377 A1 AR 127377A1
Authority
AR
Argentina
Prior art keywords
als
plant
beta vulgaris
protein
acid
Prior art date
Application number
ARP220102807A
Other languages
English (en)
Inventor
Czarnecki Olaf Dr
Gertz Maik Dr
Jens Christoph Lein
Wurbs David Dr
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of AR127377A1 publication Critical patent/AR127377A1/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/06Roots
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/123Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • A01H1/124Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance to sulfonylurea
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/501,3-Diazoles; Hydrogenated 1,3-diazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/541,3-Diazines; Hydrogenated 1,3-diazines
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647Triazoles; Hydrogenated triazoles
    • A01N43/6531,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/36Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P13/00Herbicides; Algicides
    • A01P13/02Herbicides; Algicides selective
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1022Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y202/00Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01Transketolases and transaldolases (2.2.1)
    • C12Y202/01006Acetolactate synthase (2.2.1.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Biophysics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se relaciona con plantas o partes de plantas de Beta vulgaris que tienen una proteína acetolactato sintasa (ALS) endógena mutada que comprende en la posición 371 un aminoácido diferente al aspartato (D). Dichas plantas se caracterizan por tener una mayor tolerancia a los herbicidas inhibidores de ALS. La invención se relaciona además con métodos para generar dichas plantas así como también con métodos para identificar dichas plantas. Reivindicación 1: Una planta, parte de planta, o población de plantas de Beta vulgaris que comprende, expresa o es capaz de expresar una proteína acetolactato sintasa (ALS) endógena mutada o un ácido polinucleico que codifica una proteína acetolactato sintasa (ALS) endógena mutada que comprende en la posición 371 un aminoácido diferente al ácido aspártico (D). Reivindicación 7: Un ácido polinucleico (aislado) que codifica una proteína acetolactato sintasa (ALS) endógena mutada como se define en cualquiera de las reivindicaciones 1 a 6. Reivindicación 9: Un vector que comprende el ácido polinucleico de acuerdo con la reivindicación 7 u 8. Reivindicación 10: Un ácido polinucleico (aislado) que se hibrida de forma específica con el ácido polinucleico de acuerdo con la reivindicación 7, el complemento del mismo, o el complemento inverso del mismo, con preferencia en donde dicho ácido polinucleico es un cebador o una sonda. Reivindicación 11: Un método para identificar una planta o parte de planta de Beta vulgaris, que comprende el cribado de la presencia en una proteína ALS de un aminoácido en la posición 371 que es diferente al ácido aspártico (D), o el cribado de la presencia de un codón que codifica un aminoácido en una proteína ALS en la posición 371 que es diferente al ácido aspártico (D). Reivindicación 12: Un método para generar una planta o parte de planta de Beta vulgaris, que comprende mutar en el genoma de una planta o parte de una planta un alelo ALS endógeno que da como resultado un alelo ALS que codifica una proteína ALS que comprende en la posición 371 un aminoácido diferente al ácido aspártico (D) o la introducción en el genoma (y la expresión) de una planta o de parte de una planta de un ácido polinucleico que codifica una proteína ALS endógena mutada que comprende en la posición 371 un aminoácido diferente al ácido aspártico (D). Reivindicación 14: Un método para controlar la vegetación no deseada en áreas de cultivo de Beta vulgaris o para aumentar el rendimiento en áreas de cultivo de Beta vulgaris, que comprende los pasos de: a) la plantación de plantas de Beta vulgaris o la siembra de semillas de Beta vulgaris de acuerdo con cualquiera de las reivindicaciones 1 a 6, b) la aplicación de uno o más herbicidas inhibidores de ALS a las plantas en crecimiento, con preferencia a una dosificación suficiente para inhibir el crecimiento de la vegetación no deseada, con mayor preferencia a una dosificación suficiente para eliminar la vegetación no deseada, y c) opcionalmente, la repetición del paso b) durante la temporada de cultivo.
ARP220102807A 2021-10-15 2022-10-14 Mutantes de beta vulgaris tolerantes a herbicidas inhibidores de als AR127377A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP21202922 2021-10-15

Publications (1)

Publication Number Publication Date
AR127377A1 true AR127377A1 (es) 2024-01-17

Family

ID=78371783

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP220102807A AR127377A1 (es) 2021-10-15 2022-10-14 Mutantes de beta vulgaris tolerantes a herbicidas inhibidores de als

Country Status (8)

Country Link
US (1) US20240401073A1 (es)
EP (1) EP4415527A1 (es)
JP (1) JP2024537389A (es)
CN (1) CN118201483A (es)
AR (1) AR127377A1 (es)
CA (1) CA3234200A1 (es)
CL (1) CL2024001072A1 (es)
WO (1) WO2023062184A1 (es)

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BR112014005963A2 (pt) * 2011-09-13 2020-12-08 Monsanto Technology Llc métodos de controle de planta, de redução de expressão de um gene als em uma planta, de identificação de polinucleotídeos úteis na modulação de expressão do gene als, de fazer um polinucleotídeo, composições compreendendo um polinucleotídeo e um agente de transferência ou um herbicida, cassete de expressão microbiana, bem como molécula de polinucleotídeo
PL2931902T3 (pl) 2012-12-13 2018-11-30 Sesvanderhave N.V. Sposób opracowania roślin buraka cukrowego odpornych na herbicydy
RS57806B2 (sr) 2012-12-13 2022-07-29 Bayer Cropscience Ag Upotreba als inhibitora herbicida za kontrolu neželjene vegetacije kod beta vulgaris biljaka tolerantnih na als inhibitore herbicida

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