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AR119996A1 - Polipéptido de hidroxifenilpiruvato dioxigenasa mutante, gen codificante y su uso - Google Patents

Polipéptido de hidroxifenilpiruvato dioxigenasa mutante, gen codificante y su uso

Info

Publication number
AR119996A1
AR119996A1 ARP200102584A ARP200102584A AR119996A1 AR 119996 A1 AR119996 A1 AR 119996A1 AR P200102584 A ARP200102584 A AR P200102584A AR P200102584 A ARP200102584 A AR P200102584A AR 119996 A1 AR119996 A1 AR 119996A1
Authority
AR
Argentina
Prior art keywords
hppd
polypeptide
mutant
plants
hydroxyphenilpyruvate
Prior art date
Application number
ARP200102584A
Other languages
English (en)
Inventor
Xiang Xiao
Xiaoming Bao
Qingfang Song
Caihong Yu
Qing Tao
Original Assignee
Beijing Dabeinong Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Dabeinong Biotechnology Co Ltd filed Critical Beijing Dabeinong Biotechnology Co Ltd
Publication of AR119996A1 publication Critical patent/AR119996A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente se refiere a un polipéptido mutante de hidroxifenilpiruvato dioxigenasa, a genes codificantes y a su uso. El polipéptido de hidroxifenilpiruvato dioxigenasa mutante, que conserva la actividad de catalizar la reacción de transformación del ácido 4-hidroxifenilpirúvico en ácido homogentísico u homogentisato y es menos sensible a un herbicida inhibidor de HPPD que el HPPD nativo no mutado, comprende la siguiente mutación en una posición correspondiente a posición 372 de la secuencia de aminoácidos como se establece en la SEQ ID Nº 1: F372A, F372G, F372V, F372P, F372S, F372T, F372C, F372M, F372Q, F372D o deleción de F372. La presente describe por primera vez que las mutaciones que ocurren en la posición 372 del polipéptido de HPPD derivado de diferentes especies o diferentes ecotipos dentro de la misma especie pueden conferir una mayor tolerancia a los herbicidas inhibidores de HPPD en las plantas y, por lo tanto, tienen una perspectiva de aplicación muy amplia en plantas.
ARP200102584A 2019-09-17 2020-09-17 Polipéptido de hidroxifenilpiruvato dioxigenasa mutante, gen codificante y su uso AR119996A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2019/106168 WO2021051265A1 (zh) 2019-09-17 2019-09-17 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途

Publications (1)

Publication Number Publication Date
AR119996A1 true AR119996A1 (es) 2022-01-26

Family

ID=74883428

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP200102584A AR119996A1 (es) 2019-09-17 2020-09-17 Polipéptido de hidroxifenilpiruvato dioxigenasa mutante, gen codificante y su uso

Country Status (10)

Country Link
US (1) US20230212587A1 (es)
EP (1) EP4032976A4 (es)
CN (1) CN113574173A (es)
AR (1) AR119996A1 (es)
AU (1) AU2019466501B2 (es)
BR (1) BR112021024656A2 (es)
CA (1) CA3154740A1 (es)
IL (1) IL291089A (es)
UY (1) UY38884A (es)
WO (1) WO2021051265A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111334484B (zh) * 2020-03-19 2022-06-03 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
WO2022127894A1 (zh) * 2020-12-17 2022-06-23 上海蓝十字医学科学研究所 除草剂抗性植物
CN116064430B (zh) * 2021-09-14 2023-08-11 山东舜丰生物科技有限公司 突变的hppd多肽及其应用
CA3236161A1 (en) * 2021-12-15 2023-06-22 Beijing Dabeinong Biotechnology Co., Ltd. Mutated hydroxyphenylpyruvate dioxygenase polypeptide, and coding gene and use thereof
CN114181956B (zh) * 2022-01-26 2023-11-28 西南大学 小麦条锈病抗性相关代谢物及其合成相关基因与应用
CN114317481B (zh) * 2022-02-08 2023-10-27 宁夏农林科学院农作物研究所（宁夏回族自治区农作物育种中心） 水稻als突变型基因及其蛋白在抗除草剂方面的应用
CN119876056B (zh) * 2025-01-07 2025-11-25 中国农业科学院深圳农业基因组研究所(岭南现代农业科学与技术广东省实验室深圳分中心) 一种抗除草剂的hppd突变蛋白

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NZ217113A (en) 1985-08-07 1988-06-30 Monsanto Co Production of eucaryotic plants which are glyphosate resistant, vectors (transformation and expression), chimeric gene and plant cells
ES2018274T5 (es) 1986-03-11 1996-12-16 Plant Genetic Systems Nv Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica.
US5276268A (en) 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
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US20020058249A1 (en) 1998-08-12 2002-05-16 Venkiteswaran Subramanian Dna shuffling to produce herbicide selective crops
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CA2688682A1 (en) * 2007-05-30 2008-12-11 Syngenta Participations Ag Cytochrome p450 genes conferring herbicide resistance
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Also Published As

Publication number Publication date
CA3154740A1 (en) 2021-03-25
CN113574173A (zh) 2021-10-29
UY38884A (es) 2020-11-30
US20230212587A1 (en) 2023-07-06
AU2019466501A9 (en) 2022-05-05
EP4032976A4 (en) 2023-09-27
AU2019466501A1 (en) 2022-02-17
EP4032976A1 (en) 2022-07-27
IL291089A (en) 2022-07-01
WO2021051265A1 (zh) 2021-03-25
BR112021024656A2 (pt) 2022-04-12
AU2019466501B2 (en) 2024-07-25

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