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AR108847A1 - GENERACIÓN DE CÉLULAS MADRE PLURIPOTENTES HUMANAS DERIVADAS DE CÉLULAS FUNCIONALES b CON RESPIRACIÓN MITOCONDRIAL DEPENDIENTE DE GLUCOSA Y CON RESPUESTA EN DOS FASES DE SECRECIÓN DE INSULINA - Google Patents

GENERACIÓN DE CÉLULAS MADRE PLURIPOTENTES HUMANAS DERIVADAS DE CÉLULAS FUNCIONALES b CON RESPIRACIÓN MITOCONDRIAL DEPENDIENTE DE GLUCOSA Y CON RESPUESTA EN DOS FASES DE SECRECIÓN DE INSULINA

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Publication number
AR108847A1
AR108847A1 ARP170101710A ARP170101710A AR108847A1 AR 108847 A1 AR108847 A1 AR 108847A1 AR P170101710 A ARP170101710 A AR P170101710A AR P170101710 A ARP170101710 A AR P170101710A AR 108847 A1 AR108847 A1 AR 108847A1
Authority
AR
Argentina
Prior art keywords
glucose
cells
insulin secretion
mitocondrial
phases
Prior art date
Application number
ARP170101710A
Other languages
English (en)
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of AR108847A1 publication Critical patent/AR108847A1/es

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Abstract

La solicitud proporciona métodos para diferenciar células endocrinas pancreáticas en células beta pancreáticas que expresen PDX1, NKX6.1, MAFA, UCN3 y SLC2A. Estas células beta pancreáticas pueden obtenerse por diferenciación escalonada de células madre pluripotentes. Las células beta pancreáticas exhiben una respiración mitocondrial dependiente de glucosa y una secreción de insulina estimulada por glucosa similar a las células de los islotes.
ARP170101710A 2016-06-21 2017-06-21 GENERACIÓN DE CÉLULAS MADRE PLURIPOTENTES HUMANAS DERIVADAS DE CÉLULAS FUNCIONALES b CON RESPIRACIÓN MITOCONDRIAL DEPENDIENTE DE GLUCOSA Y CON RESPUESTA EN DOS FASES DE SECRECIÓN DE INSULINA AR108847A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201662352968P 2016-06-21 2016-06-21

Publications (1)

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AR108847A1 true AR108847A1 (es) 2018-10-03

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ARP170101710A AR108847A1 (es) 2016-06-21 2017-06-21 GENERACIÓN DE CÉLULAS MADRE PLURIPOTENTES HUMANAS DERIVADAS DE CÉLULAS FUNCIONALES b CON RESPIRACIÓN MITOCONDRIAL DEPENDIENTE DE GLUCOSA Y CON RESPUESTA EN DOS FASES DE SECRECIÓN DE INSULINA

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US (3) US10633635B2 (es)
EP (2) EP3472303B1 (es)
JP (4) JP7044726B2 (es)
KR (5) KR102607813B1 (es)
CN (2) CN109415693B (es)
AR (1) AR108847A1 (es)
AU (3) AU2017282638C1 (es)
CA (1) CA3027879A1 (es)
DK (1) DK3472303T3 (es)
ES (1) ES2939233T3 (es)
FI (1) FI4194548T3 (es)
LT (1) LT4194548T (es)
MA (2) MA71715A (es)
RU (1) RU2752499C2 (es)
SG (1) SG11201810550WA (es)
TW (1) TW201809266A (es)
WO (1) WO2017222879A1 (es)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018285539B2 (en) 2017-06-14 2024-10-31 Vertex Pharmaceuticals Incorporated Devices and methods for delivering therapeutics
CA3074910A1 (en) * 2017-09-11 2019-03-14 Novo Nordisk A/S Enrichment of nkx6.1 and c-peptide co-expressing cells derived in vitro from stem cells
WO2019099725A1 (en) 2017-11-15 2019-05-23 Semma Therapeutics, Inc. Islet cell manufacturing compositions and methods of use
EP3758719A4 (en) * 2018-03-02 2021-12-08 Vertex Pharmaceuticals Inc. METHODS FOR IMPROVING THE DIFFERENTIATION OF STEM CELLS INTO BETA CELLS
WO2019208788A1 (ja) * 2018-04-27 2019-10-31 株式会社カネカ 膵臓β細胞の製造方法
CA3099770A1 (en) * 2018-05-07 2019-11-14 President And Fellows Of Harvard College Cell populations and gene expression associated with in vitro beta cell differentiation
EP3790956A4 (en) 2018-05-11 2022-03-16 Cornell University METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS INTO MONOHORMONAL CELLS
EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
AU2020283056B2 (en) 2019-05-31 2023-06-08 Viacyte, Inc. A biocompatible membrane composite
CA3257300A1 (en) 2019-05-31 2025-06-12 W. L. Gore & Associates, Inc. Cell encapsulation devices with controlled oxygen diffusion distances
AU2020284245B2 (en) 2019-05-31 2023-10-05 Viacyte, Inc. A biocompatible membrane composite
EP3976236A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. A biocompatible membrane composite
JP2022539169A (ja) * 2019-06-25 2022-09-07 バーテックス ファーマシューティカルズ インコーポレイテッド ベータ細胞の分化の向上
US12479852B2 (en) 2019-06-27 2025-11-25 Dana-Farber Cancer Institute, Inc. Compounds, compositions, and methods for protein degradation
AU2020340622A1 (en) 2019-09-05 2022-03-03 Crispr Therapeutics Ag Universal donor cells
US20230014430A1 (en) * 2019-11-20 2023-01-19 Washington University Methods and Compositions for Generating Functionally Mature Beta Cells and Uses Thereof
WO2021233116A1 (zh) * 2020-05-21 2021-11-25 北京大学 多能干细胞的成分明确、无异源的培养系统
AU2021319209A1 (en) 2020-07-31 2023-03-02 Vertex Pharmaceuticals Incorporated Differentiation of pancreatic endocrine cells
CA3203392A1 (en) 2020-12-31 2022-07-07 Alireza Rezania Universal donor cells
CN114958726B (zh) * 2021-02-23 2025-03-21 海门雨霖细胞科技有限责任公司 化学诱导成纤维细胞重编程为肺干细胞的化学小分子组合物及其应用
US20240182864A1 (en) * 2021-03-24 2024-06-06 Salk Institute For Biological Studies Novel chemical combinations and methods of use thereof, towards differentiation of human progenitor cells into functional beta cells
WO2022236173A1 (en) * 2021-05-07 2022-11-10 Mayo Foundation For Medical Education And Research Treating liver disease
US20220403340A1 (en) * 2021-06-11 2022-12-22 Washington University Media for cryopreserved cells and methods of making and using same
WO2023077140A2 (en) 2021-11-01 2023-05-04 Vertex Pharmaceuticals Incorporated Stem cell derived pancreatic islet differentiation
CN114317401B (zh) * 2021-12-07 2022-10-04 浙江大学 一种促进胰岛前体细胞长期稳定传代的方法
KR102848180B1 (ko) * 2021-12-15 2025-08-19 한국화학연구원 오로라 키나아제 억제제를 이용하는 심근세포 및 심장 오가노이드의 제조방법
WO2025117331A1 (en) 2023-12-01 2025-06-05 Eli Lilly And Company Methods of making stem cell-derived islet-like cells, as well as populations and compositions including the same
WO2025119299A1 (en) * 2023-12-08 2025-06-12 Hangzhou Reprogenix Bioscience, Inc. Method for differentiating pancreatic beta cells
WO2025202874A1 (en) 2024-03-25 2025-10-02 Crispr Therapeutics Ag Genetically modified cells expressing glp-1 receptor agonist

Family Cites Families (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
ES2568463T3 (es) 2003-06-27 2016-04-29 DePuy Synthes Products, Inc. Regeneración y reparación de tejido neural usando células del postparto derivadas del cordon umbilical
WO2007059007A2 (en) * 2005-11-14 2007-05-24 Cythera, Inc. Markers of definitive endoderm
SG149061A1 (en) 2003-12-23 2009-01-29 Cythera Inc Definitive endoderm
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
WO2006105152A2 (en) 2005-03-31 2006-10-05 Stemnion, Inc. Amnion-derived cell compositions, methods of making and uses thereof
AU2006202209B2 (en) 2005-05-27 2011-04-14 Lifescan, Inc. Amniotic fluid derived cells
US7807459B2 (en) 2005-09-27 2010-10-05 Reneuron, Inc. EphA4-positive human adult pancreatic endocrine progenitor cells
US7695965B2 (en) 2006-03-02 2010-04-13 Cythera, Inc. Methods of producing pancreatic hormones
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US20090269313A1 (en) * 2006-07-19 2009-10-29 Diakine Therapeutics, Inc. Encapsulation system
AU2007277364B2 (en) 2006-07-26 2010-08-12 Viacyte, Inc. Methods of producing pancreatic hormones
WO2009070592A2 (en) 2007-11-27 2009-06-04 Lifescan, Inc. Differentiation of human embryonic stem cells
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
WO2009132083A2 (en) 2008-04-22 2009-10-29 President And Fellows Of Harvard College Compositions and methods for promoting the generation of pdx1+ pancreatic cells
KR20180018839A (ko) 2008-06-30 2018-02-21 얀센 바이오테크 인코포레이티드 만능 줄기 세포의 분화
RU2011103183A (ru) 2008-06-30 2012-08-10 Сентокор Орто Байотек Инк. (Us) Дифференцирование плюрипотентных стволовых клеток
CN102272291B (zh) 2008-10-31 2018-01-16 詹森生物科技公司 人胚胎干细胞向胰腺内分泌谱系的分化
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
ES2665006T3 (es) 2009-10-29 2018-04-24 Janssen Biotech, Inc. Células madre pluripotentes
CA2784415C (en) 2009-12-23 2019-06-18 Jean Xu Differentiation of human embryonic stem cells
NZ607608A (en) 2010-08-09 2014-03-28 Takeda Pharmaceuticals Co Method of producing pancreatic hormone-producing cells
CN105143446B (zh) * 2011-12-22 2020-11-03 詹森生物科技公司 人胚胎干细胞分化成单一激素胰岛素阳性细胞
KR102285014B1 (ko) * 2012-06-08 2021-08-03 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 췌장 내분비 세포로의 분화
WO2014033322A1 (en) 2012-09-03 2014-03-06 Novo Nordisk A/S Generation of pancreatic endoderm from pluripotent stem cells using small molecules
US9902938B2 (en) * 2012-12-26 2018-02-27 Kyoto University Method for inducing exocrine pancreatic cells
EP4219683A1 (en) 2012-12-31 2023-08-02 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endocrine cells using hb9 regulators
RU2658488C2 (ru) 2012-12-31 2018-06-21 Янссен Байотек, Инк. Способ получения клеток, экспрессирующих маркеры, характерные для панкреатических эндокринных клеток
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
KR20150103203A (ko) 2012-12-31 2015-09-09 얀센 바이오테크 인코포레이티드 췌장 내분비 세포 내로의 분화를 위한 인간 만능 세포의 현탁 및 클러스터링
US20140329704A1 (en) * 2013-03-28 2014-11-06 President And Fellows Of Harvard College Markers for mature beta-cells and methods of using the same
RU2692595C2 (ru) * 2013-06-11 2019-06-25 Президент Энд Феллоус Оф Гарвард Колледж КЛЕТКИ SC-β И КОМПОЗИЦИИ И СПОСОБЫ ДЛЯ ИХ СОЗДАНИЯ
EP3143127B1 (en) 2014-05-16 2021-07-14 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
CA3010610A1 (en) * 2016-01-08 2017-07-13 The Brigham And Women's Hospital, Inc. Production of differentiated enteroendocrine cells and insulin producing cells
MA45747A (fr) 2016-02-24 2019-01-02 Novo Nordisk As Génération de cellules bêta fonctionnelles à partir de progéniteurs endocrines dérivés de cellules souhes pluripotentes humaines

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