RU2011103183A - Дифференцирование плюрипотентных стволовых клеток - Google Patents
Дифференцирование плюрипотентных стволовых клеток Download PDFInfo
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- RU2011103183A RU2011103183A RU2011103183/10A RU2011103183A RU2011103183A RU 2011103183 A RU2011103183 A RU 2011103183A RU 2011103183/10 A RU2011103183/10 A RU 2011103183/10A RU 2011103183 A RU2011103183 A RU 2011103183A RU 2011103183 A RU2011103183 A RU 2011103183A
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- amino acid
- point mutation
- stem cells
- pluripotent stem
- activin
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- 210000001778 pluripotent stem cell Anatomy 0.000 title claims abstract 9
- 230000004069 differentiation Effects 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 16
- 230000035772 mutation Effects 0.000 claims abstract 12
- 108010023082 activin A Proteins 0.000 claims abstract 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 8
- 125000000539 amino acid group Chemical group 0.000 claims abstract 4
- 210000004027 cell Anatomy 0.000 claims abstract 4
- 230000037430 deletion Effects 0.000 claims abstract 4
- 238000012217 deletion Methods 0.000 claims abstract 4
- 210000001900 endoderm Anatomy 0.000 claims abstract 4
- 230000037431 insertion Effects 0.000 claims abstract 4
- 238000003780 insertion Methods 0.000 claims abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims abstract 2
- 238000001261 affinity purification Methods 0.000 claims 2
- 239000003446 ligand Substances 0.000 claims 2
- 239000002184 metal Substances 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 claims 1
- 238000006467 substitution reaction Methods 0.000 abstract 2
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Peptides Or Proteins (AREA)
Abstract
1. Способ дифференцирования плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы, состоящий в обработке плюрипотентных стволовых клеток средой, содержащей пептид с аминокислотной последовательностью активин А, имеющей по меньшей мере одну точечную мутацию, в течение периода времени, достаточного для того, чтобы плюрипотентные стволовые клетки дифференцировались в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы. !2. Способ по п.1, в котором указанные плюрипотентные стволовые клетки представляют собой эмбриональные стволовые клетки. ! 3. Способ по п.1, в котором по меньшей мере одну точечную мутацию вводят в аминокислотную последовательность активина А по месту по меньшей мере одного аминокислотного остатка, выбираемого из группы, состоящей из: 10I, 16F, 39Y, 41E, 43E, 74F, 75A, 76N, 77L, 78K, 79S и 82V. ! 4. Способ по п.3, в котором указанную по меньшей мере одну точечную мутацию выбирают из группы, состоящей из: делеции, инсерции и замены. ! 5. Способ по п.1, в котором по меньшей мере одну точечную мутацию вводят в аминокислотную последовательность активина А по месту по меньшей мере одного аминокислотного остатка, выбираемого из группы, состоящей из: 16F, 18V, 19S, 20F, 37A, 38N, 39Y, 41E, 74F, 82V, 107N, 109I, 110V и 116S. ! 6. Способ по п.5, в котором указанную по меньшей мере одну точечную мутацию выбирают из группы, состоящей из: делеции, инсерции и замены. ! 7. Способ по п.1, в котором пептид, содержащий аминокислотную последовательность активина А, содержащую по меньшей мере одну точечную мутацию, подвергается дальнейшей модификации для включения по меньшей мере одного участка
Claims (9)
1. Способ дифференцирования плюрипотентных стволовых клеток в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы, состоящий в обработке плюрипотентных стволовых клеток средой, содержащей пептид с аминокислотной последовательностью активин А, имеющей по меньшей мере одну точечную мутацию, в течение периода времени, достаточного для того, чтобы плюрипотентные стволовые клетки дифференцировались в клетки, экспрессирующие маркеры, характерные для линии сформированной эндодермы.
2. Способ по п.1, в котором указанные плюрипотентные стволовые клетки представляют собой эмбриональные стволовые клетки.
3. Способ по п.1, в котором по меньшей мере одну точечную мутацию вводят в аминокислотную последовательность активина А по месту по меньшей мере одного аминокислотного остатка, выбираемого из группы, состоящей из: 10I, 16F, 39Y, 41E, 43E, 74F, 75A, 76N, 77L, 78K, 79S и 82V.
4. Способ по п.3, в котором указанную по меньшей мере одну точечную мутацию выбирают из группы, состоящей из: делеции, инсерции и замены.
5. Способ по п.1, в котором по меньшей мере одну точечную мутацию вводят в аминокислотную последовательность активина А по месту по меньшей мере одного аминокислотного остатка, выбираемого из группы, состоящей из: 16F, 18V, 19S, 20F, 37A, 38N, 39Y, 41E, 74F, 82V, 107N, 109I, 110V и 116S.
6. Способ по п.5, в котором указанную по меньшей мере одну точечную мутацию выбирают из группы, состоящей из: делеции, инсерции и замены.
7. Способ по п.1, в котором пептид, содержащий аминокислотную последовательность активина А, содержащую по меньшей мере одну точечную мутацию, подвергается дальнейшей модификации для включения по меньшей мере одного участка, способного специфически связываться с лигандом, находящимся на твердом субстрате в колонке для аффинной очистки.
8. Способ по п.7, в котором по меньшей мере один участок, способный специфически связываться с лигандом, находящимся на твердом субстрате в колонке для аффинной очистки, представляет собой сайт связывания с металлом.
9. Способ по п.8, в котором сайт связывания с металлом содержит пару остатков гистидина.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7688908P | 2008-06-30 | 2008-06-30 | |
| US61/076,889 | 2008-06-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2011103183A true RU2011103183A (ru) | 2012-08-10 |
Family
ID=41226604
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2011103183/10A RU2011103183A (ru) | 2008-06-30 | 2009-06-29 | Дифференцирование плюрипотентных стволовых клеток |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20110091971A1 (ru) |
| EP (1) | EP2318516A1 (ru) |
| JP (1) | JP2011526784A (ru) |
| KR (1) | KR20110025220A (ru) |
| CN (2) | CN108486040A (ru) |
| AU (1) | AU2009267167A1 (ru) |
| BR (1) | BRPI0913925A2 (ru) |
| CA (1) | CA2729734A1 (ru) |
| MX (1) | MX2011000123A (ru) |
| RU (1) | RU2011103183A (ru) |
| WO (1) | WO2010002785A1 (ru) |
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| EP2367931B1 (en) * | 2008-12-03 | 2017-05-31 | International Stem Cell Corporation | Methods of deriving differentiated cells from stem cells |
| CN108220224A (zh) | 2011-06-21 | 2018-06-29 | 诺沃—诺迪斯克有限公司 | 自多潜能干细胞有效诱导定形内胚层 |
| ES2897649T3 (es) * | 2012-06-08 | 2022-03-02 | Janssen Biotech Inc | Diferenciación de células madre embrionarias humanas en células endocrinas pancreáticas |
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| DK2938723T3 (da) | 2012-12-31 | 2023-02-20 | Janssen Biotech Inc | Differentiering af humane embryonale stamceller til pancreatiske endokrine celler under anvendelse af hb9-regulatorer |
| GB201317869D0 (en) | 2013-10-09 | 2013-11-20 | Cambridge Entpr Ltd | In vitro production of foregut stem cells |
| CN106414720A (zh) | 2014-05-16 | 2017-02-15 | 詹森生物科技公司 | 小分子增强胰腺内分泌细胞中的mafa表达的用途 |
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| DE102004043256B4 (de) * | 2004-09-07 | 2013-09-19 | Rheinische Friedrich-Wilhelms-Universität Bonn | Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension |
| GB2432835B (en) * | 2004-09-08 | 2009-04-08 | Wisconsin Alumni Res Found | Culturing human embryonic stem cells |
| CN101052712A (zh) * | 2004-09-08 | 2007-10-10 | 威斯康星校友研究基金会 | 培养人胚胎干细胞 |
| US20060194321A1 (en) * | 2005-01-31 | 2006-08-31 | Alan Colman | Directed differentiation of embryonic stem cells and uses thereof |
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| US8716018B2 (en) * | 2008-03-17 | 2014-05-06 | Agency For Science, Technology And Research | Microcarriers for stem cell culture |
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| AU2009267137A1 (en) * | 2008-06-30 | 2010-01-07 | Centocor Ortho Biotech Inc. | Differentiation of pluripotent stem cells |
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| US8008075B2 (en) * | 2008-11-04 | 2011-08-30 | Viacyte, Inc. | Stem cell aggregate suspension compositions and methods of differentiation thereof |
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2009
- 2009-06-29 JP JP2011516763A patent/JP2011526784A/ja active Pending
- 2009-06-29 CN CN201810244535.1A patent/CN108486040A/zh active Pending
- 2009-06-29 CA CA2729734A patent/CA2729734A1/en not_active Abandoned
- 2009-06-29 WO PCT/US2009/049049 patent/WO2010002785A1/en not_active Ceased
- 2009-06-29 EP EP20090774236 patent/EP2318516A1/en not_active Withdrawn
- 2009-06-29 BR BRPI0913925-7A patent/BRPI0913925A2/pt not_active Application Discontinuation
- 2009-06-29 KR KR1020117001977A patent/KR20110025220A/ko not_active Withdrawn
- 2009-06-29 RU RU2011103183/10A patent/RU2011103183A/ru not_active Application Discontinuation
- 2009-06-29 MX MX2011000123A patent/MX2011000123A/es active IP Right Grant
- 2009-06-29 AU AU2009267167A patent/AU2009267167A1/en not_active Abandoned
- 2009-06-29 CN CN200980134985.4A patent/CN102171330B/zh not_active Expired - Fee Related
- 2009-06-29 US US12/493,741 patent/US20110091971A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| MX2011000123A (es) | 2011-02-25 |
| CA2729734A1 (en) | 2010-01-07 |
| JP2011526784A (ja) | 2011-10-20 |
| KR20110025220A (ko) | 2011-03-09 |
| AU2009267167A1 (en) | 2010-01-07 |
| US20110091971A1 (en) | 2011-04-21 |
| WO2010002785A1 (en) | 2010-01-07 |
| CN102171330B (zh) | 2018-04-20 |
| CN102171330A (zh) | 2011-08-31 |
| EP2318516A1 (en) | 2011-05-11 |
| CN108486040A (zh) | 2018-09-04 |
| BRPI0913925A2 (pt) | 2015-08-04 |
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| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
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