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AR107872A1 - Proteinasa b y solución de lactasa usando sus propiedades y método para producirlo - Google Patents

Proteinasa b y solución de lactasa usando sus propiedades y método para producirlo

Info

Publication number
AR107872A1
AR107872A1 ARP170100629A ARP170100629A AR107872A1 AR 107872 A1 AR107872 A1 AR 107872A1 AR P170100629 A ARP170100629 A AR P170100629A AR P170100629 A ARP170100629 A AR P170100629A AR 107872 A1 AR107872 A1 AR 107872A1
Authority
AR
Argentina
Prior art keywords
lactase
proteinase
approximately
properties
produce
Prior art date
Application number
ARP170100629A
Other languages
English (en)
Inventor
Jun Yoshikawa
Asami Sugawara
Original Assignee
Godo Sushei Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Godo Sushei Co Ltd filed Critical Godo Sushei Co Ltd
Publication of AR107872A1 publication Critical patent/AR107872A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Proporcionar una solución de lactasa con buena estabilidad térmica según se desea por identificación de un factor para influir sobre la estabilidad térmica en un producto de lactasa y uso del factor como un índice. Proteinasa B con las siguientes propiedades enzimáticas y una solución de lactasa que contiene menos de 11,5 ng de la proteinasa B; 1) que tiene una temperatura óptima de aproximadamente 40ºC, 2) que tiene un pH óptimo de aproximadamente 8,0, 3) que es estable a pH 5,0 - 8,0, 4) que tiene alta especificidad de sustrato por FITC-caseína y lactasa, 5) que tiene una acción de fragmentación de lactasa neutra, y 6) que tiene un peso molecular de aproximadamente 29.700 a 30.000 (por SDS-PAGE).
ARP170100629A 2016-03-16 2017-03-14 Proteinasa b y solución de lactasa usando sus propiedades y método para producirlo AR107872A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2016053138 2016-03-16

Publications (1)

Publication Number Publication Date
AR107872A1 true AR107872A1 (es) 2018-06-13

Family

ID=59851263

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP170100629A AR107872A1 (es) 2016-03-16 2017-03-14 Proteinasa b y solución de lactasa usando sus propiedades y método para producirlo

Country Status (5)

Country Link
US (1) US20190075806A1 (es)
EP (1) EP3431596A4 (es)
JP (1) JPWO2017159723A1 (es)
AR (1) AR107872A1 (es)
WO (1) WO2017159723A1 (es)

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6018394B2 (ja) 1976-08-19 1985-05-10 合同酒精株式会社 微生物によるラクタ−ゼの製造法
US4329429A (en) * 1980-03-24 1982-05-11 Pfizer Inc. Lactase preparation
EP0119329A1 (en) 1982-12-28 1984-09-26 Unilever N.V. Treatment of milk products with lactic acid bacteria and lactose-splitting enzymes, and use of the resulting products in preparing foodstuffs
JP2623807B2 (ja) * 1987-03-05 1997-06-25 東レ株式会社 セリンプロテアーゼおよびセリンプロテアーゼ遺伝子
KR920702814A (ko) 1990-07-30 1992-10-28 죤 엠. 샌드레 저칼로리 유제품 혼합물
FR2692907B1 (fr) * 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
JPH08256770A (ja) * 1995-03-24 1996-10-08 Green Cross Corp:The ピキア属酵母由来のプロテアーゼ
WO2002081673A1 (en) * 2001-04-04 2002-10-17 Dsm Ip Assets B.V. Purified lactase
WO2007060247A2 (en) * 2005-11-28 2007-05-31 Dsm Ip Assets B.V. Enzyme preparations yielding a clean taste
JP2010241708A (ja) * 2009-04-02 2010-10-28 Yashima Shoji Kk エラスチン含有可溶性ペプチド及びその製造方法
WO2014185364A1 (ja) * 2013-05-13 2014-11-20 合同酒精株式会社 ラクターゼ含有組成物の製造法
JP6842915B6 (ja) 2016-12-28 2021-04-14 マーレベーアサーマルシステムズジャパン株式会社 エバポレータ

Also Published As

Publication number Publication date
EP3431596A4 (en) 2019-09-25
WO2017159723A1 (ja) 2017-09-21
JPWO2017159723A1 (ja) 2019-01-24
EP3431596A1 (en) 2019-01-23
US20190075806A1 (en) 2019-03-14

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