AR107366A1 - Composición y métodos para la crioconservación de hutc - Google Patents
Composición y métodos para la crioconservación de hutcInfo
- Publication number
- AR107366A1 AR107366A1 ARP170100102A ARP170100102A AR107366A1 AR 107366 A1 AR107366 A1 AR 107366A1 AR P170100102 A ARP170100102 A AR P170100102A AR P170100102 A ARP170100102 A AR P170100102A AR 107366 A1 AR107366 A1 AR 107366A1
- Authority
- AR
- Argentina
- Prior art keywords
- umbilical cord
- cord tissue
- composition
- express
- cells derived
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title abstract 24
- 238000000034 method Methods 0.000 title abstract 7
- 210000001519 tissue Anatomy 0.000 abstract 19
- 210000003954 umbilical cord Anatomy 0.000 abstract 19
- 210000004027 cell Anatomy 0.000 abstract 14
- 238000005138 cryopreservation Methods 0.000 abstract 10
- 238000001816 cooling Methods 0.000 abstract 6
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 abstract 5
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 abstract 5
- 108010017842 Telomerase Proteins 0.000 abstract 5
- 210000004369 blood Anatomy 0.000 abstract 5
- 239000008280 blood Substances 0.000 abstract 5
- 238000004519 manufacturing process Methods 0.000 abstract 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 abstract 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 3
- 238000009472 formulation Methods 0.000 abstract 3
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 abstract 3
- -1 salts potassium chloride Chemical class 0.000 abstract 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 abstract 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 2
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 abstract 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 abstract 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 abstract 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 abstract 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 abstract 2
- 235000015497 potassium bicarbonate Nutrition 0.000 abstract 2
- 229910000028 potassium bicarbonate Inorganic materials 0.000 abstract 2
- 239000011736 potassium bicarbonate Substances 0.000 abstract 2
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 abstract 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 abstract 2
- QDGAVODICPCDMU-UHFFFAOYSA-N 2-amino-3-[3-[bis(2-chloroethyl)amino]phenyl]propanoic acid Chemical compound OC(=O)C(N)CC1=CC=CC(N(CCCl)CCCl)=C1 QDGAVODICPCDMU-UHFFFAOYSA-N 0.000 abstract 1
- 239000001763 2-hydroxyethyl(trimethyl)azanium Substances 0.000 abstract 1
- UOQHWNPVNXSDDO-UHFFFAOYSA-N 3-bromoimidazo[1,2-a]pyridine-6-carbonitrile Chemical compound C1=CC(C#N)=CN2C(Br)=CN=C21 UOQHWNPVNXSDDO-UHFFFAOYSA-N 0.000 abstract 1
- 102100022464 5'-nucleotidase Human genes 0.000 abstract 1
- 102100022749 Aminopeptidase N Human genes 0.000 abstract 1
- 102100032912 CD44 antigen Human genes 0.000 abstract 1
- 102000009410 Chemokine receptor Human genes 0.000 abstract 1
- 108050000299 Chemokine receptor Proteins 0.000 abstract 1
- 235000019743 Choline chloride Nutrition 0.000 abstract 1
- 102000050083 Class E Scavenger Receptors Human genes 0.000 abstract 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 abstract 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 abstract 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 1
- 108010024636 Glutathione Proteins 0.000 abstract 1
- 239000004471 Glycine Substances 0.000 abstract 1
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 abstract 1
- 101000678236 Homo sapiens 5'-nucleotidase Proteins 0.000 abstract 1
- 101000757160 Homo sapiens Aminopeptidase N Proteins 0.000 abstract 1
- 101000868273 Homo sapiens CD44 antigen Proteins 0.000 abstract 1
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 abstract 1
- 101000800116 Homo sapiens Thy-1 membrane glycoprotein Proteins 0.000 abstract 1
- 102000004890 Interleukin-8 Human genes 0.000 abstract 1
- 108090001007 Interleukin-8 Proteins 0.000 abstract 1
- 239000007836 KH2PO4 Substances 0.000 abstract 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 abstract 1
- 229930064664 L-arginine Natural products 0.000 abstract 1
- 235000014852 L-arginine Nutrition 0.000 abstract 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 abstract 1
- 229930182816 L-glutamine Natural products 0.000 abstract 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 abstract 1
- 229930182844 L-isoleucine Natural products 0.000 abstract 1
- 239000004395 L-leucine Substances 0.000 abstract 1
- 235000019454 L-leucine Nutrition 0.000 abstract 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 abstract 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 abstract 1
- 229930195725 Mannitol Natural products 0.000 abstract 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 abstract 1
- 102000003729 Neprilysin Human genes 0.000 abstract 1
- 108090000028 Neprilysin Proteins 0.000 abstract 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 abstract 1
- 102100024616 Platelet endothelial cell adhesion molecule Human genes 0.000 abstract 1
- 102100022647 Reticulon-1 Human genes 0.000 abstract 1
- 101710122684 Reticulon-1 Proteins 0.000 abstract 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 abstract 1
- 239000004473 Threonine Substances 0.000 abstract 1
- 102100033523 Thy-1 membrane glycoprotein Human genes 0.000 abstract 1
- 229940024606 amino acid Drugs 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 210000002798 bone marrow cell Anatomy 0.000 abstract 1
- FAPWYRCQGJNNSJ-UBKPKTQASA-L calcium D-pantothenic acid Chemical compound [Ca+2].OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O.OCC(C)(C)[C@@H](O)C(=O)NCCC([O-])=O FAPWYRCQGJNNSJ-UBKPKTQASA-L 0.000 abstract 1
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 abstract 1
- 229940052299 calcium chloride dihydrate Drugs 0.000 abstract 1
- 230000003399 chemotactic effect Effects 0.000 abstract 1
- SGMZJAMFUVOLNK-UHFFFAOYSA-M choline chloride Chemical compound [Cl-].C[N+](C)(C)CCO SGMZJAMFUVOLNK-UHFFFAOYSA-M 0.000 abstract 1
- 229960003178 choline chloride Drugs 0.000 abstract 1
- 239000008121 dextrose Substances 0.000 abstract 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 abstract 1
- 210000002950 fibroblast Anatomy 0.000 abstract 1
- 229960000304 folic acid Drugs 0.000 abstract 1
- 235000019152 folic acid Nutrition 0.000 abstract 1
- 239000011724 folic acid Substances 0.000 abstract 1
- 229960001031 glucose Drugs 0.000 abstract 1
- 229960003180 glutathione Drugs 0.000 abstract 1
- 210000003714 granulocyte Anatomy 0.000 abstract 1
- XKTZWUACRZHVAN-VADRZIEHSA-N interleukin-8 Chemical compound C([C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@@H](NC(C)=O)CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCSC)C(=O)N1[C@H](CCC1)C(=O)N1[C@H](CCC1)C(=O)N[C@@H](C)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@H](CC(O)=O)C(=O)N[C@H](CC=1C=CC(O)=CC=1)C(=O)N[C@H](CO)C(=O)N1[C@H](CCC1)C(N)=O)C1=CC=CC=C1 XKTZWUACRZHVAN-VADRZIEHSA-N 0.000 abstract 1
- 229940096397 interleukin-8 Drugs 0.000 abstract 1
- 229960000310 isoleucine Drugs 0.000 abstract 1
- 229940099563 lactobionic acid Drugs 0.000 abstract 1
- 229960003136 leucine Drugs 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 229910001629 magnesium chloride Inorganic materials 0.000 abstract 1
- DHRRIBDTHFBPNG-UHFFFAOYSA-L magnesium dichloride hexahydrate Chemical compound O.O.O.O.O.O.[Mg+2].[Cl-].[Cl-] DHRRIBDTHFBPNG-UHFFFAOYSA-L 0.000 abstract 1
- 239000000594 mannitol Substances 0.000 abstract 1
- 229960001855 mannitol Drugs 0.000 abstract 1
- 235000010355 mannitol Nutrition 0.000 abstract 1
- 210000002901 mesenchymal stem cell Anatomy 0.000 abstract 1
- 229940045641 monobasic sodium phosphate Drugs 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- 229960003966 nicotinamide Drugs 0.000 abstract 1
- 235000005152 nicotinamide Nutrition 0.000 abstract 1
- 239000011570 nicotinamide Substances 0.000 abstract 1
- 239000001103 potassium chloride Substances 0.000 abstract 1
- 235000011164 potassium chloride Nutrition 0.000 abstract 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M potassium chloride Inorganic materials [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 235000008160 pyridoxine Nutrition 0.000 abstract 1
- 239000011677 pyridoxine Substances 0.000 abstract 1
- 229960002477 riboflavin Drugs 0.000 abstract 1
- 235000019192 riboflavin Nutrition 0.000 abstract 1
- 239000002151 riboflavin Substances 0.000 abstract 1
- 108091005418 scavenger receptor class E Proteins 0.000 abstract 1
- 229960001153 serine Drugs 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 abstract 1
- WPLOVIFNBMNBPD-ATHMIXSHSA-N subtilin Chemical compound CC1SCC(NC2=O)C(=O)NC(CC(N)=O)C(=O)NC(C(=O)NC(CCCCN)C(=O)NC(C(C)CC)C(=O)NC(=C)C(=O)NC(CCCCN)C(O)=O)CSC(C)C2NC(=O)C(CC(C)C)NC(=O)C1NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C1NC(=O)C(=C/C)/NC(=O)C(CCC(N)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C2NC(=O)CNC(=O)C3CCCN3C(=O)C(NC(=O)C3NC(=O)C(CC(C)C)NC(=O)C(=C)NC(=O)C(CCC(O)=O)NC(=O)C(NC(=O)C(CCCCN)NC(=O)C(N)CC=4C5=CC=CC=C5NC=4)CSC3)C(C)SC2)C(C)C)C(C)SC1)CC1=CC=CC=C1 WPLOVIFNBMNBPD-ATHMIXSHSA-N 0.000 abstract 1
- 239000005720 sucrose Substances 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 229960003495 thiamine Drugs 0.000 abstract 1
- 235000019157 thiamine Nutrition 0.000 abstract 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 abstract 1
- 239000011721 thiamine Substances 0.000 abstract 1
- 229960002898 threonine Drugs 0.000 abstract 1
- 229960004799 tryptophan Drugs 0.000 abstract 1
- 229940088594 vitamin Drugs 0.000 abstract 1
- 235000013343 vitamin Nutrition 0.000 abstract 1
- 239000011782 vitamin Substances 0.000 abstract 1
- 229930003231 vitamin Natural products 0.000 abstract 1
- 229940011671 vitamin b6 Drugs 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Pregnancy & Childbirth (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Physiology (AREA)
- Biophysics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Reivindicación 1: Una composición para la crioconservación de células derivadas de tejido de cordón umbilical, en donde la composición comprende: los aminoácidos L-arginina, L-glutamina, glicina, L-isoleucina, L-leucina, L-lisina, HCl, L-metionina, L-fenilalanina, L-serina, L-treonina, L-triptofano y L-valina; las vitaminas D-calcio pantotenato, cloruro de colina, ácido fólico, niacinamida, piridoxina, HCl, riboflavina y tiamina, HCl; las sales inorgánicas cloruro de potasio, USP, bicarbonato de sodio, USP, cloruro de sodio, USP y fosfato de sodio, monobásico, H₂O; las sales inorgánicas dihidrato de cloruro de calcio (CaCl₂.2H₂O), cloruro de magnesio (MgCl₂.6H₂O), bicarbonato de potasio (KHCO₃) y monofosfato de potasio (KH₂PO₄) y DMSO, ácido lactobiónico, glutatión, dextrosa, sacarosa y manitol. Reivindicación 7: La composición de la reivindicación 1, en donde las células derivadas de tejido de cordón umbilical humano se aíslan de tejido de cordón umbilical humano sustancialmente libre de sangre, tienen la capacidad de autorrenovación y de expansión en cultivo, tienen el potencial de diferenciarse, carecen la producción de CD117 o CD45, expresan y no expresan hTERT o telomerasa. Reivindicación 8: La composición de la reivindicación 7, en donde las células derivadas de tejido del cordón umbilical comprenden, además, una o más de las siguientes características: expresan el receptor de lipoproteína de baja densidad oxidado 1, reticulón, ligando del receptor de quimocina 3 y/o proteína quimiotáctica de no granulocitos; expresan CD31 o CD34; expresan, en relación con un fibroblasto humano, células madre mesenquimales o células de la médula ósea de la cresta iliaca, niveles elevados de interleucina 8 o reticulón 1; y expresan CD10, CD13, CD44, CD73 y CD90. Reivindicación 9: Un estuche que comprende la composición de la reivindicación 1 y células derivadas de tejido del cordón umbilical, en donde las células derivadas de tejido de cordón umbilical humano se aíslan de tejido de cordón umbilical humano sustancialmente libre de sangre, tienen la capacidad de autorrenovación y de expansión en cultivo, tienen el potencial de diferenciarse, carecen la producción de CD117 o CD45, expresan y no expresan hTERT o telomerasa. Reivindicación 13: Un método para crioconservar células derivadas de tejido de cordón umbilical humano, el método comprende: proporcionar una formulación que comprende la composición de crioconservación de la reivindicación 1 y células derivadas del tejido del cordón umbilical; enfriar la composición de crioconservación de una temperatura inicial de aproximadamente 4ºC a una velocidad de 1,0ºC/min. hasta que la composición alcance una temperatura de aproximadamente -45,0ºC; y además, enfriar la composición de crioconservación hasta que la composición alcance una temperatura de almacenamiento de aproximadamente -120,0ºC; en donde las células derivadas de tejido de cordón umbilical humano se aíslan de tejido de cordón umbilical humano sustancialmente libre de sangre, tienen la capacidad de autorrenovación y de expansión en cultivo, tienen el potencial de diferenciarse, carecen la producción de CD117 o CD45, expresan y no expresan hTERT o telomerasa. Reivindicación 17: Un método para crioconservar células derivadas de tejido de cordón umbilical humano, el método comprende: proporcionar una formulación que comprende la composición de crioconservación de la reivindicación 3 y células derivadas del tejido del cordón umbilical enfriar la composición de crioconservación de una temperatura inicial de aproximadamente 4ºC a una velocidad de -1,0ºC/min. hasta que la composición alcance una temperatura de aproximadamente -45,0ºC; y además, enfriar la composición de crioconservación hasta que la composición alcance una temperatura de almacenamiento de aproximadamente -120,0ºC; en donde las células derivadas de tejido de cordón umbilical humano se aíslan de tejido de cordón umbilical humano sustancialmente libre de sangre, tienen la capacidad de autorrenovación y de expansión en cultivo, tienen el potencial de diferenciarse, carecen la producción de CD117 o CD45, expresan y no expresan hTERT o telomerasa. Reivindicación 19: Un método para crioconservar células derivadas de tejido de cordón umbilical humano, el método comprende: proporcionar una formulación que comprende la composición de crioconservación de la reivindicación 6 y células derivadas del tejido del cordón umbilical; enfriar la composición de crioconservación de una temperatura inicial de aproximadamente 4ºC a una velocidad de -1,0ºC/min. hasta que la composición alcance una temperatura de aproximadamente -45,0ºC; y además, enfriar la composición de crioconservación hasta que la composición alcance una temperatura de almacenamiento de aproximadamente -120,0ºC; en donde las células derivadas de tejido de cordón umbilical humano se aíslan de tejido de cordón umbilical humano sustancialmente libre de sangre, tienen la capacidad de autorrenovación y de expansión en cultivo, tienen el potencial de diferenciarse, carecen la producción de CD117 o CD45, expresan y no expresan hTERT o telomerasa.
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| CN107683849A (zh) * | 2017-10-12 | 2018-02-13 | 北京臻惠康生物科技有限公司 | 一种直接静脉应用的间充质干细胞冻存液、制备方法及其使用方法 |
| RU2707921C1 (ru) * | 2018-12-10 | 2019-12-02 | Федеральное государственное бюджетное учреждение науки "Кировский научно-исследовательский институт гематологии и переливания крови Федерального медико-биологического агентства" | Способ нерегламентированного замораживания клеток-предшественников периферической крови при температуре минус 80оС под защитой диметилсульфоксида |
| CN113453548A (zh) * | 2019-02-14 | 2021-09-28 | 北哥罗夫投资公司 | 用于保持活的和静态的生物材料的生存力的组合物、其制造方法及用途 |
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| CN112889813B (zh) * | 2021-01-29 | 2022-10-18 | 华夏源细胞工程集团股份有限公司 | 一种细胞存活率高的人脐带间充质干细胞的冻存方法 |
| CN112913833B (zh) * | 2021-01-29 | 2022-10-14 | 华夏源细胞工程集团股份有限公司 | 一种人脐带间充质干细胞工作细胞库的程序降温方法 |
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| CN114762500A (zh) * | 2021-05-14 | 2022-07-19 | 达瑟儿(上海)生命科技有限公司 | 一种高存活率的细胞冻存方法 |
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- 2017-01-06 KR KR1020187023353A patent/KR102211270B1/ko not_active Expired - Fee Related
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- 2017-01-06 EP EP17700764.8A patent/EP3402329A1/en not_active Withdrawn
- 2017-01-06 CN CN201780006784.0A patent/CN108471736A/zh active Pending
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| AU2017207707B2 (en) | 2020-12-17 |
| RU2018129431A (ru) | 2020-02-14 |
| SG11201805533QA (en) | 2018-07-30 |
| RU2748057C2 (ru) | 2021-05-19 |
| EP3402329A1 (en) | 2018-11-21 |
| JP6855489B2 (ja) | 2021-04-07 |
| CN108471736A (zh) | 2018-08-31 |
| RU2018129431A3 (es) | 2020-10-14 |
| US10791730B2 (en) | 2020-10-06 |
| AU2017207707A1 (en) | 2018-07-12 |
| WO2017123465A1 (en) | 2017-07-20 |
| JP2019503181A (ja) | 2019-02-07 |
| KR20180100681A (ko) | 2018-09-11 |
| CA3010792A1 (en) | 2017-07-20 |
| KR102211270B1 (ko) | 2021-02-04 |
| MX2018008712A (es) | 2019-06-06 |
| US20170202212A1 (en) | 2017-07-20 |
| BR112018014238A2 (pt) | 2018-12-11 |
| PH12018501414A1 (en) | 2019-01-28 |
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