AR092482A1 - Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas - Google Patents
Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantasInfo
- Publication number
- AR092482A1 AR092482A1 ARP130103193A ARP130103193A AR092482A1 AR 092482 A1 AR092482 A1 AR 092482A1 AR P130103193 A ARP130103193 A AR P130103193A AR P130103193 A ARP130103193 A AR P130103193A AR 092482 A1 AR092482 A1 AR 092482A1
- Authority
- AR
- Argentina
- Prior art keywords
- etip
- plant
- facs
- enrichment
- classification
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 abstract 1
- 108091033409 CRISPR Proteins 0.000 abstract 1
- 238000010354 CRISPR gene editing Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108010017070 Zinc Finger Nucleases Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000011218 segmentation Effects 0.000 abstract 1
- 230000009261 transgenic effect Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
- C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
- C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Botany (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
Abstract
Se describe una Plataforma de Integración del Transgen realizado por Ingeniería (ETIP) que se puede insertar al azar, o en ubicaciones específicas en el genoma de las plantas para facilitar la rápida selección y detección de un GOI que está perfectamente dirigido (ambos extremos 3 y 5) en la localización ETIP genómica. Un elemento es la introducción de roturas de doble cadena específicas dentro de la ETIP. En algunas formas de realización, se describe una ETIP que utiliza sitios de unión de nucleasa de dedo de zinc, pero puede utilizar otras tecnologías de segmentación tales como meganucleasas, TALS, CRISPRs, o cremalleras de leucina. También se describen composiciones de, y métodos para producir, las plantas transgénicas en las que el donante o el ADN de carga útil expresa uno o más productos de una secuencia de ácido nucleico exógena (por ejemplo, proteína o ARN) que ha sido de manera estable integrada en una ETIP en una célula de la planta. En realizaciones, la ETIP facilita la prueba de candidatos de genes y vectores de expresión de plantas para la ideación por medios de fases de Desarrollo. Planta.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261697890P | 2012-09-07 | 2012-09-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR092482A1 true AR092482A1 (es) | 2015-04-22 |
Family
ID=50234835
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP130103193A AR092482A1 (es) | 2012-09-07 | 2013-09-06 | Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20140075593A1 (es) |
| EP (1) | EP2893024B1 (es) |
| JP (1) | JP6362600B2 (es) |
| KR (1) | KR102219621B1 (es) |
| CN (2) | CN111118100A (es) |
| AR (1) | AR092482A1 (es) |
| AU (1) | AU2013312198B2 (es) |
| BR (1) | BR112015004864B8 (es) |
| CA (1) | CA2883800C (es) |
| HK (1) | HK1210220A1 (es) |
| IL (1) | IL237535B (es) |
| IN (1) | IN2015DN01785A (es) |
| MX (1) | MX367170B (es) |
| RU (1) | RU2679510C2 (es) |
| TW (1) | TWI670004B (es) |
| WO (1) | WO2014039970A1 (es) |
| ZA (1) | ZA201501736B (es) |
Families Citing this family (46)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| EP2893006B1 (en) | 2012-09-07 | 2018-08-22 | Dow AgroSciences LLC | Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
| UA119135C2 (uk) | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| WO2015070083A1 (en) | 2013-11-07 | 2015-05-14 | Editas Medicine,Inc. | CRISPR-RELATED METHODS AND COMPOSITIONS WITH GOVERNING gRNAS |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| KR102763527B1 (ko) | 2014-12-03 | 2025-02-05 | 애질런트 테크놀로지스, 인크. | 화학적 변형을 갖는 가이드 rna |
| ES2884838T3 (es) | 2015-04-06 | 2021-12-13 | Univ Leland Stanford Junior | ARN guía químicamente modificados para la regulación génica mediada por CRISPR/CAS |
| US10167457B2 (en) | 2015-10-23 | 2019-01-01 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
| CN106198358B (zh) * | 2016-06-24 | 2019-02-15 | 北京大学 | 基于"石英杯中激发型"流式细胞分选仪的染色体分选方法 |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| EP3497214B1 (en) | 2016-08-09 | 2023-06-28 | President and Fellows of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| EP3510145A4 (en) | 2016-09-06 | 2020-03-25 | The Children's Medical Center Corporation | IMMUNCELLS FROM INDUCED PLURIPOTENT STEM CELLS |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| BR112019019655A2 (pt) | 2017-03-23 | 2020-04-22 | Harvard College | editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| GB201708662D0 (en) | 2017-05-31 | 2017-07-12 | Tropic Biosciences Uk Ltd | Compositions and methods for increasing shelf-life of banana |
| EP3630973A1 (en) | 2017-05-31 | 2020-04-08 | Tropic Biosciences UK Limited | Methods of selecting cells comprising genome editing events |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| US11555199B2 (en) | 2017-09-19 | 2023-01-17 | Tropic Biosciences UK Limited | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
| AU2018352592C1 (en) | 2017-10-16 | 2025-09-25 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| CN114127285B (zh) | 2019-03-19 | 2024-09-10 | 布罗德研究所股份有限公司 | 编辑核苷酸序列的方法和组合物 |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| GB201905542D0 (en) * | 2019-04-18 | 2019-06-05 | Phytoform Labs Ltd | Methods, systems and apparatus for plant material screening and propagation |
| WO2021067394A1 (en) * | 2019-09-30 | 2021-04-08 | Calyxt, Inc. | Gene editing using a messenger ribonucleic acid construct |
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| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
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| CN120082500B (zh) * | 2025-04-28 | 2026-01-27 | 北京艾普希隆生物科技有限公司 | 一种制备植物单细胞的方法 |
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2013
- 2013-09-06 AR ARP130103193A patent/AR092482A1/es unknown
- 2013-09-06 TW TW102132254A patent/TWI670004B/zh not_active IP Right Cessation
- 2013-09-06 US US14/020,694 patent/US20140075593A1/en not_active Abandoned
- 2013-09-09 IN IN1785DEN2015 patent/IN2015DN01785A/en unknown
- 2013-09-09 BR BR112015004864A patent/BR112015004864B8/pt active IP Right Grant
- 2013-09-09 EP EP13835630.8A patent/EP2893024B1/en active Active
- 2013-09-09 MX MX2015002964A patent/MX367170B/es active IP Right Grant
- 2013-09-09 CA CA2883800A patent/CA2883800C/en active Active
- 2013-09-09 AU AU2013312198A patent/AU2013312198B2/en active Active
- 2013-09-09 HK HK15111003.5A patent/HK1210220A1/xx unknown
- 2013-09-09 JP JP2015531282A patent/JP6362600B2/ja active Active
- 2013-09-09 KR KR1020157008557A patent/KR102219621B1/ko not_active Expired - Fee Related
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| EP2893024B1 (en) | 2019-12-04 |
| IL237535A0 (en) | 2015-04-30 |
| IN2015DN01785A (es) | 2015-05-29 |
| MX2015002964A (es) | 2015-06-05 |
| BR112015004864B1 (pt) | 2022-10-25 |
| MX367170B (es) | 2019-08-07 |
| WO2014039970A9 (en) | 2014-10-09 |
| US20140075593A1 (en) | 2014-03-13 |
| CA2883800A1 (en) | 2014-03-13 |
| CA2883800C (en) | 2021-07-13 |
| EP2893024A1 (en) | 2015-07-15 |
| AU2013312198B2 (en) | 2018-11-08 |
| TW201424578A (zh) | 2014-07-01 |
| AU2013312198A1 (en) | 2015-03-19 |
| JP2015527085A (ja) | 2015-09-17 |
| BR112015004864A2 (pt) | 2017-11-21 |
| HK1210220A1 (en) | 2016-04-15 |
| KR102219621B1 (ko) | 2021-02-24 |
| ZA201501736B (en) | 2016-10-26 |
| KR20150052241A (ko) | 2015-05-13 |
| EP2893024A4 (en) | 2016-03-30 |
| CN104736712A (zh) | 2015-06-24 |
| RU2015112573A (ru) | 2016-10-27 |
| CN111118100A (zh) | 2020-05-08 |
| IL237535B (en) | 2019-11-28 |
| BR112015004864B8 (pt) | 2023-05-16 |
| TWI670004B (zh) | 2019-09-01 |
| JP6362600B2 (ja) | 2018-07-25 |
| WO2014039970A1 (en) | 2014-03-13 |
| RU2679510C2 (ru) | 2019-02-11 |
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