[go: up one dir, main page]

AR098875A1 - Medios para cultivo celular - Google Patents

Medios para cultivo celular

Info

Publication number
AR098875A1
AR098875A1 ARP140104810A ARP140104810A AR098875A1 AR 098875 A1 AR098875 A1 AR 098875A1 AR P140104810 A ARP140104810 A AR P140104810A AR P140104810 A ARP140104810 A AR P140104810A AR 098875 A1 AR098875 A1 AR 098875A1
Authority
AR
Argentina
Prior art keywords
medium
phosphatidylcholine
cholesterol
adherent culture
liposomes
Prior art date
Application number
ARP140104810A
Other languages
English (en)
Original Assignee
Essential Pharmaceuticals Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Essential Pharmaceuticals Llc filed Critical Essential Pharmaceuticals Llc
Publication of AR098875A1 publication Critical patent/AR098875A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/34—Sugars
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/35—Polyols, e.g. glycerin, inositol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/32—Polylysine, polyornithine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Biophysics (AREA)
  • Physiology (AREA)

Abstract

La presente descripción se refiere, a un medio, por ejemplo, un reemplazo de suero, suplemento de medio, medio completo o medio de criopreservación, que comprende un tampón fisiológico básico y liposomas que comprenden colesterol, fosfatidilcolina y ácidos grasos. Se contempla que el medio provea ventajas para mejorar el crecimiento celular en cultivo en comparación con células cultivadas sin usar el reemplazo de suero descrito en la presente. Reivindicación 1: Un medio para usar con células en suspensión o en cultivo adherente, que comprende una mezcla líquida de tampón fisiológico básico y (a) liposomas que comprenden colesterol, fosfatidilcolina y ácidos grasos, en donde el liposoma está en una cantidad tal que la concentración final de colesterol en una suspensión celular o cultivo adherente va de 1 a 20 mg/L y en donde Ia concentración final de fosfatidilcolina en una suspensión celular o cultivo adherente va de 100 a 1000 mg/L; o (b) pectina; o (a) y (b).
ARP140104810A 2013-12-20 2014-12-19 Medios para cultivo celular AR098875A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361918833P 2013-12-20 2013-12-20

Publications (1)

Publication Number Publication Date
AR098875A1 true AR098875A1 (es) 2016-06-22

Family

ID=53399356

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP140104810A AR098875A1 (es) 2013-12-20 2014-12-19 Medios para cultivo celular

Country Status (16)

Country Link
US (2) US9637721B2 (es)
EP (1) EP3083978A4 (es)
JP (1) JP2017500893A (es)
KR (1) KR20160125352A (es)
CN (1) CN106062205A (es)
AR (1) AR098875A1 (es)
AU (1) AU2014364368A1 (es)
BR (1) BR112016014488A2 (es)
CA (1) CA2934487A1 (es)
CL (1) CL2016001553A1 (es)
EA (1) EA201691297A1 (es)
IL (1) IL246262A0 (es)
MX (1) MX2016008251A (es)
SG (1) SG11201604994UA (es)
TW (1) TW201527534A (es)
WO (1) WO2015095651A1 (es)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10918102B2 (en) 2014-03-13 2021-02-16 The General Hospital Corporation Devices and methods to improve and assess viability of human livers
US11891622B2 (en) 2014-12-30 2024-02-06 Cell Cure Neurosciences Ltd. RPE cell populations and methods of generating same
US11732241B2 (en) 2015-10-15 2023-08-22 Wake Forest University Health Sciences Methods of producing in vitro liver constructs and uses thereof
AU2016339998B2 (en) 2015-10-16 2022-04-07 Wake Forest University Health Sciences Multi-layer airway organoids and methods of making and using the same
US20180369336A1 (en) * 2015-12-03 2018-12-27 Boston Medical Center Corporation B cell-based cancer immunotherapy
CN105567628B (zh) * 2016-01-30 2019-07-12 令世鑫 一种全悬浮培养mdck细胞的低血清培养基
CN105695407B (zh) * 2016-03-15 2019-04-16 佰通生物技术(苏州)有限公司 一种对干细胞具有活化作用的微量元素组合物及其应用
CN108779436A (zh) 2016-03-18 2018-11-09 中国科学院生物物理研究所 一种细胞培养基和培养基补充物
TW202140776A (zh) 2016-04-26 2021-11-01 美商美國泰福生技股份有限公司 細胞培養基
CN106119186B (zh) * 2016-06-24 2019-10-08 肇庆大华农生物药品有限公司 一种用于全悬浮培养mdck细胞的无血清培养基及其制备方法
CN106497872B (zh) * 2016-12-01 2019-10-11 北京欣博睿丰医疗科技发展有限公司 骨骼肌干细胞无血清培养基及其制备方法和应用
US12087399B2 (en) * 2017-02-01 2024-09-10 Sapphiros Ai Bio Llc Systems and methods for customizing cell culture media for optimized cell proliferation based on genetic traits of cells
CA3058306A1 (en) * 2017-03-28 2018-10-04 Ajinomoto Co., Inc. Additive for undifferentiation maintaining medium
CN107177542A (zh) * 2017-05-25 2017-09-19 句容亿格纳米材料厂 一种促进培养基中谷氨酰胺溶解的优化方法
ES2955861T3 (es) * 2017-05-31 2023-12-07 Promocell Gmbh Medio de cultivo para células madre pluripotentes
US12058996B2 (en) * 2017-06-14 2024-08-13 The General Hospital Corporation High subzero cryopreservation
CN107488625B (zh) * 2017-08-31 2018-12-21 广州蕊特生物科技有限公司 一种胎牛血清替代物及其制备方法和应用
CN109395095B (zh) * 2017-09-18 2021-09-24 武汉原生原代生物医药科技有限公司 体内用生物膜及其制备方法和用途
BR112020007083A2 (pt) * 2017-10-11 2020-09-24 Bast Biotechnology composições e métodos para tratamento de fibrose
JP7117374B2 (ja) * 2017-10-13 2022-08-12 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 灌流培地
WO2019081609A1 (en) * 2017-10-24 2019-05-02 Fundación Instituto De Investigación Sanitaria De Santiago De Compostela (Fidis) NANOSYSTEMS FOR CELL PROLIFERATION
KR102569522B1 (ko) * 2017-11-24 2023-08-24 주식회사 차바이오랩 세포 동결 보존용 조성물 및 이를 이용한 세포 동결 보존 방법
JP7498664B2 (ja) * 2017-12-29 2024-06-12 セル キュア ニューロサイエンシズ リミテッド 網膜色素上皮細胞組成物
US12167729B2 (en) 2018-05-30 2024-12-17 The General Hospital Corporation Cryopreservation of tissues and organs
CN108853489B (zh) * 2018-06-29 2019-07-02 陕西诺威利华生物科技有限公司 一种利用无血清培养基生产pedv弱毒疫苗的方法
CN108815516B (zh) * 2018-06-29 2019-03-15 陕西诺威利华生物科技有限公司 一种利用无血清培养基生产pedv灭活疫苗的方法
CN109234223B (zh) * 2018-11-21 2021-01-19 南京基蛋生物医药有限公司 低蛋白无血清细胞培养基
KR102271949B1 (ko) * 2019-04-15 2021-07-02 순천향대학교 산학협력단 포스파티딜콜린을 함유하는 선충 배양용 조성물 및 이를 이용한 선충의 배양방법
WO2020218584A1 (ja) * 2019-04-26 2020-10-29 味の素株式会社 細胞培養用組成物
KR102132457B1 (ko) * 2019-05-10 2020-07-09 주식회사 바이오솔루션 세포외 소포를 고농도로 배양하기 위한 세포 배양배지 및 상기 세포 배양배지를 이용한 세포외 소포 고 함유 조건화 배양액의 제조방법
CN109943526B (zh) * 2019-05-23 2019-09-13 广州赛莱拉干细胞科技股份有限公司 一种促间充质干细胞增殖的无血清多肽组合物
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
WO2021078935A1 (en) * 2019-10-25 2021-04-29 Fundació Centre De Regulació Genòmica Culture media for mycoplasma
CN114786481A (zh) * 2019-11-29 2022-07-22 新泽西鲁特格斯州立大学 用于储存血液制品的组合物、试剂盒和方法及其使用方法
EP4093852A1 (en) * 2020-01-21 2022-11-30 Yissum Research Development Company of the Hebrew University of Jerusalem Ltd Utilization of fgf activators in culture media
CN111849860B (zh) * 2020-06-01 2021-12-21 浙江大学 一种利用铁元素调控肠道干细胞分化的方法及应用
IL298798A (en) * 2020-06-05 2023-02-01 Upside Foods Inc Nutrient media for the production of slaughter-free meat
CN111534478A (zh) * 2020-06-24 2020-08-14 肇庆大华农生物药品有限公司 用于培养mdck细胞系的培养基及其制备方法
US20220142150A1 (en) * 2020-11-10 2022-05-12 Axogen Corporation Packaging system for a medical product
CN112514892B (zh) * 2020-12-25 2022-06-14 广州赛莱拉干细胞科技股份有限公司 一种外泌体冻存保护液及其制备方法
CN112501113B (zh) * 2021-02-03 2021-04-27 上海奥浦迈生物科技股份有限公司 一种hek293细胞组合梯度培养基及其用途
CN113061584A (zh) * 2021-05-14 2021-07-02 赵峻岭 一种能促进腺病毒生长的培养基添加物
EP4381045A4 (en) * 2021-08-02 2025-06-04 Montana State University Chemically defined serum replacements for cell culture
US11878036B2 (en) 2022-05-25 2024-01-23 Neuvian LLC Vaginal care compositions and methods of improving vaginal health
WO2024024892A1 (ja) * 2022-07-28 2024-02-01 公立大学法人山陽小野田市立山口東京理科大学 凍結保存用の組成物、凍結保存方法、及び凍結した細胞又は生体組織
KR20240177180A (ko) * 2023-06-19 2024-12-27 (주)엑셀세라퓨틱스 T 세포 배양용 배지 조성물
WO2025098952A1 (en) 2023-11-07 2025-05-15 Evonik Operations Gmbh Nutrient preloading of cells
WO2025199245A1 (en) * 2024-03-19 2025-09-25 Immunis, Inc. Method of making and using secretome composition
US12421441B1 (en) 2025-05-19 2025-09-23 King Fahd University Of Petroleum And Minerals Method for microbially enhanced oil recovery

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4721676A (en) 1982-12-14 1988-01-26 Genencor, Inc. Novel thermophile isolate having thermostable hydrolytic activity
US4560655A (en) 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
DE3827578A1 (de) 1988-08-13 1990-02-15 Siepmann Friedrich W Verfahren und vorrichtung zur bestimmung oxidierbarer wasserinhaltsstoffe einer waessrigen probenfluessigkeit
US6048728A (en) 1988-09-23 2000-04-11 Chiron Corporation Cell culture medium for enhanced cell growth, culture longevity, and product expression
US5834312A (en) 1990-01-29 1998-11-10 Hy-Gene, Inc. Process and media for the growth of human epithelia
US5952198A (en) * 1995-05-04 1999-09-14 Bayer Corporation Production of recombinant Factor VIII in the presence of liposome-like substances of mixed composition
WO1997006784A1 (en) 1995-08-15 1997-02-27 Universite Libre De Bruxelles Liposomes preparation method and plant
CA2235069C (en) * 1995-10-19 2010-12-14 Advanced Reproduction Technologies, Inc. Methods and compositions to improve germ cell and embryo survival and function
EP1482031B1 (en) 1996-08-30 2015-10-28 Life Technologies Corporation Serum-free mammalian cell culture medium, and uses thereof
WO1998015614A1 (en) 1996-10-10 1998-04-16 Life Technologies, Inc. Animal cell culture media comprising plant-derived nutrients
US6833271B2 (en) * 1996-12-04 2004-12-21 Medi-Cult A/S Serum-free cell culture media
US5985538A (en) 1997-08-01 1999-11-16 Saint Barnabas Medical Center Cryopreservation and cell culture medium comprising less than 50 mM sodium ions and greater than 100 mM choline salt
GB9817805D0 (en) 1998-08-14 1998-10-14 Danisco Use of a composition
NZ517002A (en) 1999-08-05 2004-06-25 Mcl Llc Multipotent adult stem cells and methods for isolation
US7112576B1 (en) * 1999-12-10 2006-09-26 Regents Of The University Of Minnesota Compositions and methods for cryopreservation of peripheral blood lymphocytes
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
CA2473360A1 (en) 2002-01-25 2003-08-07 Genzyme Corporation Serum-free media for chondrocytes and methods of use thereof
EP1475434A1 (en) * 2003-05-09 2004-11-10 Oncoscience AG Method for storing tumor cells
EP1711053A2 (en) 2004-02-02 2006-10-18 I.M.T. Interface Multigrad Technology Ltd. Biological material and methods and solutions for preservation thereof
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
US20050287666A1 (en) 2004-06-29 2005-12-29 Invitrogen Corporation Cell culture medium comprising transition metals or trace elements
EP2267116B1 (en) 2004-07-13 2017-05-31 Asterias Biotherapeutics, Inc. Growth medium for primate embryonic stem cells
WO2006045064A2 (en) 2004-10-20 2006-04-27 Whitehead Institute For Biomedical Research Cultured hematopoietic stem cells and method for expansion and analysis thereof
PL1807504T3 (pl) 2004-11-02 2011-07-29 Ares Trading Sa Bezsurowicza pożywka hodowlana dla komórek ssaczych
EP1841856A1 (en) * 2005-01-28 2007-10-10 Canadian Blood Services A method of cryopreserving cells and tissues by liposomal delivery of sugars to enhance post-thaw viability
US9101590B2 (en) 2005-07-29 2015-08-11 Yale University Defined culture conditions of human embryonic stem cells
JP5205275B2 (ja) 2005-12-21 2013-06-05 セントクローネ インターナショナル エービー 散在性ガンの治療法
CN101410509B (zh) 2006-02-23 2016-05-18 维亚赛特公司 用于培养可分化细胞的组合物和方法
JP5080779B2 (ja) 2006-10-25 2012-11-21 テルモ株式会社 リポソーム製剤の製造方法
EP2155862A2 (en) 2007-05-04 2010-02-24 Whitehead Institute For Biomedical Research Ex vivo expansion of human hematopoietic stem cells
EP2188367A4 (en) 2007-08-10 2010-10-27 Whitehead Biomedical Inst HORMON'S TREATIVE TISSUE CULTURE SYSTEM AND USES THEREOF
FI20096288A0 (fi) * 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
CN103958665A (zh) * 2011-11-11 2014-07-30 基本药品有限责任公司 包含血清替代物和不稳定因子的试剂盒

Also Published As

Publication number Publication date
KR20160125352A (ko) 2016-10-31
IL246262A0 (en) 2016-07-31
BR112016014488A2 (pt) 2017-08-08
AU2014364368A1 (en) 2016-07-07
TW201527534A (zh) 2015-07-16
MX2016008251A (es) 2016-10-13
US20150175956A1 (en) 2015-06-25
CL2016001553A1 (es) 2017-03-17
CN106062205A (zh) 2016-10-26
EP3083978A4 (en) 2017-08-30
JP2017500893A (ja) 2017-01-12
CA2934487A1 (en) 2015-06-25
EP3083978A1 (en) 2016-10-26
US9637721B2 (en) 2017-05-02
US20170198251A1 (en) 2017-07-13
WO2015095651A1 (en) 2015-06-25
EA201691297A1 (ru) 2016-11-30
SG11201604994UA (en) 2016-07-28

Similar Documents

Publication Publication Date Title
AR098875A1 (es) Medios para cultivo celular
MX2019010226A (es) Composiciones de nanoparticulas lipidicas y metodos para administracion de arnm.
BR112015001620A2 (pt) composição de meio de cultura, e método para cultivo de célula ou tecido usando-se dita composição
MX2013003681A (es) Ácidos nucleicos manipulados y métodos de uso de los mismos.
MX2018010529A (es) Composiciones y metodos para la produccion y administracion de acido ribonucleico.
ES2523998T3 (es) Método de aislamiento de citoblastos dérmicos
PH12014501019A1 (en) Kit comprising serum replacement and labile factors
IN2015KN00666A (es)
AU2011327239A8 (en) Stem cell suspension
MX2015007361A (es) Produccion mediada por la proteina portadora de acilo de los derivados de acidos grasos.
EP2633036A4 (en) DIFFERENTIATION METHOD FOR THE PRODUCTION OF GLIA CELL POPULATIONS
BR112014006244A2 (pt) microrganismos e métodos para produzir alcenos
SG196784A1 (en) Stem cell cultures
DOP2014000297A (es) Método para producir iduronato-2-sulfatasa recombinante
BR112016004510A2 (pt) composições bioativas deriváveis de concentrados de plaquetas e métodos para preparar e usar as mesmas
EA201590550A1 (ru) Композиции и способы получения гликопротеинов
BR112015009229A2 (pt) composição tendo como alvo a expressão de genes para plaquetas, célula tronco hematopoiética, uso da referida célula tronco e método in vitro ou ex vivo para gerar uma célula tronco modificada
AR082943A1 (es) Formula nutricional predigerida
AR092630A1 (es) Metodos y composiciones para prevenir la incorporacion erronea de norleucina en proteinas
AR086201A1 (es) Hongos filamentosos que tienen un fenotipo de viscosidad alterado
AR096705A1 (es) Composición que comprende ácido glutámico-n,n-diacetato (glda), agua y enzima
BR112014011198A2 (pt) dispositivos e métodos para aumento da viabiliade de bactérias em forma de bastonete
WO2011097242A3 (en) Methods of isolating and culturing mesenchymal stem cells
AU340977S (en) Carburetor float bowl
MX2014004241A (es) Composicion y metodo de fitonutrientes para efectos de programacion metabolica.

Legal Events

Date Code Title Description
FB Suspension of granting procedure