[go: up one dir, main page]

AR094337A1 - Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasas - Google Patents

Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasas

Info

Publication number
AR094337A1
AR094337A1 ARP130105096A ARP130105096A AR094337A1 AR 094337 A1 AR094337 A1 AR 094337A1 AR P130105096 A ARP130105096 A AR P130105096A AR P130105096 A ARP130105096 A AR P130105096A AR 094337 A1 AR094337 A1 AR 094337A1
Authority
AR
Argentina
Prior art keywords
seq
nucleic acid
group
pest
compositions
Prior art date
Application number
ARP130105096A
Other languages
English (en)
Original Assignee
Empresa Brasileira De Pesquisa Agropecuária - Embrapa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Empresa Brasileira De Pesquisa Agropecuária - Embrapa filed Critical Empresa Brasileira De Pesquisa Agropecuária - Embrapa
Publication of AR094337A1 publication Critical patent/AR094337A1/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Insects & Arthropods (AREA)
  • Food Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente solicitud está relacionada con el control de la infestación de plagas a través de la inhibición o reducción de la expresión de genes de la familia de la quitina sintasa. La solicitud provee además métodos y composiciones para el control de plagas, a través de la alimentación de una o más moléculas de ARN de doble hebra provista por la presente. La solicitud describe además un método de obtención de plantas transgénicas que expresen moléculas de ARN de doble hebra. La presente solicitud se utiliza preferentemente para plantas de algodonero. Reivindicación 1: Molécula de ácido nucleico aislada, caracterizada porque es seleccionada del grupo que consiste en: a) molécula de ácido nucleico aislada que comprende una secuencia de ácido nucleico sustancialmente similar a las secuencias seleccionadas del grupo de SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5 y SEQ ID Nº 6; b) molécula de ácido nucleico aislada que hibrida con una secuencia de ácidos nucleicos indicada por las secuencias seleccionadas del grupo de SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5 y SEQ ID Nº 6, bajo condiciones de lavado de 5X SSC, formamida al 50% y 42ºC; durante 10 min; c) fragmento de por lo menos 19 nucleótidos contiguos de una secuencia de ácidos nucleicos seleccionada del grupo de SEQ ID Nº 1, SEQ ID Nº 2, SEQ ID Nº 3, SEQ ID Nº 4, SEQ ID Nº 5 y SEQ ID Nº 6, donde la ingestión, por coleópteros plaga de plantas, de la secuencia de un ribonucleótido de filamento doble, que comprende por lo menos un filamento complementario del fragmento mencionado, inhibe o reduce la proliferación de la plaga mencionada; y d) complemento de la secuencia de (a), (b) ó (c).
ARP130105096A 2012-12-28 2013-12-27 Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasas AR094337A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BR102012033539A BR102012033539A8 (pt) 2012-12-28 2012-12-28 método e composições para controle genético de insetos-praga em plantas de algodão através do silenciamento de genes de quitina sintases

Publications (1)

Publication Number Publication Date
AR094337A1 true AR094337A1 (es) 2015-07-29

Family

ID=51022142

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP130105096A AR094337A1 (es) 2012-12-28 2013-12-27 Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasas

Country Status (4)

Country Link
US (1) US10182571B2 (es)
AR (1) AR094337A1 (es)
BR (1) BR102012033539A8 (es)
WO (1) WO2014100879A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3003429A1 (en) * 2015-10-28 2017-05-04 Dow Agrosciences Llc Nucleic acid molecules that confer resistance to coleopteran pests
BR112020027078A2 (pt) 2018-07-03 2021-03-30 Embrapa-Empresa Brasileira De Pesquisa Agropecuaria Nanopartículas de quitosana que têm rna aderido, processo para fabricar as referidas nanopartículas, e suspensão concentrada
CN119874868A (zh) * 2025-01-13 2025-04-25 贵州省烟草公司遵义市公司 蠋蝽多巴胺D2受体AcDD2R基因及其激活剂与应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080168583A1 (en) * 1999-08-19 2008-07-10 Fincher Karen L Nucleic acid molecules and other molecules associated with plants

Also Published As

Publication number Publication date
BR102012033539A8 (pt) 2021-08-24
US10182571B2 (en) 2019-01-22
WO2014100879A8 (pt) 2014-09-04
BR102012033539A2 (pt) 2015-08-18
US20160000086A1 (en) 2016-01-07
WO2014100879A3 (pt) 2014-11-06
WO2014100879A2 (pt) 2014-07-03

Similar Documents

Publication Publication Date Title
AR055169A1 (es) Metodos para el control genetico de infestaciones de insectos en plantas y composiciones para los mismos
CO2017003434A2 (es) Moléculas de ácido nucleico de la subunidad alfa del coatómero copi que confiere resistencia contra plagas de coleópteros y hemípteros
CO2017003428A2 (es) Moléculas de acido nucléico de sub-unidades de coatómero copi delta que confieren resistencia a plagas de coleópteros y hemípteros
AR084776A1 (es) Moleculas de acido nucleico que se dirigen a la subunidad h de la atpasa vacuolar y otorgan resistencia a las pestes de coleopteros
CO2017003543A2 (es) Moleculas de acido nucleico de la subunidad beta coatomero copi que confieren resistencia a plagas de coleopteros y hemipteros
AR088263A1 (es) Moleculas de acido nucleico que se dirigen a rps6 y confieren resistencia a plagas de coleopteros
CO2017003427A2 (es) Moleculas de acido nucleico de la subunidad gamma coatomero copi que confieren resistencia a plagas de coleopteros y hemipteros.
AR097980A1 (es) Evento de maíz transgénico mon87403 y métodos para su detección
AR109207A1 (es) Control de plagas de coleópteros utilizando moléculas de arn
AR088113A1 (es) ARN DE INTERFERENCIA (ARNi) PARA EL CONTROL DE HONGOS Y OOMICETOS POR LA INHIBICION DEL GEN DE SACAROPINA DESHIDROGENASA
CY1121037T1 (el) Αντινοηματικα πολυνουκλεοτιδια για να διεγειρεται παραλειψη εξονιου και μεθοδοι θεραπειας δυστροφιων
AR087206A1 (es) Evento de maiz mzdt09y
AR065283A1 (es) Composiciones y metodos que utilizan arn de interferencia del gen tipo opr3 para el control de nematodos
AR065286A1 (es) Composiciones y metodos que utilizan arn de interferencia destinado a genes de tipo mthfr para el control de nematodos
ES2570962T3 (es) Producción incrementada de un producto diana vía estabilización del ARNm
ES2623454T3 (es) Reducción del contenido de ácidos grasos saturados en semillas de plantas
AR069821A1 (es) Composiciones y metodos de uso de la interferencia de arn para el control de nematodos
AR065284A1 (es) Composiciones y metodos que utilizan arn de interferencia de tipo cdpk para el control de nematodos
AR065282A1 (es) Composiciones y metodos que utilizan arn de transferencia de genes tipo cad para el control de nematodos
AR109206A1 (es) Control de plagas de coleópteros utilizando moléculas de arn
AR100946A1 (es) Composiciones y métodos para regular la expresión génica por medio de la interferencia de arn
PE20211238A1 (es) Tratamiento antisentido del sindrome de angelman
AR094337A1 (es) Método y composiciones para el control genético de insectos plaga en plantas de algodón a través del silenciamiento de genes de quitina sintasas
AR083445A1 (es) siARN CONTRA LA FIBROSIS
AR103921A1 (es) Moléculas de ácido nucleico de ii33 de arn polimerasa para el control de plagas de insectos

Legal Events

Date Code Title Description
FG Grant, registration