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AR083981A1 - Cepa de bacteria recombinante fijadora de nitrogeno, inoculo que la contiene y metodos de aplicacion - Google Patents

Cepa de bacteria recombinante fijadora de nitrogeno, inoculo que la contiene y metodos de aplicacion

Info

Publication number
AR083981A1
AR083981A1 ARP110104381A ARP110104381A AR083981A1 AR 083981 A1 AR083981 A1 AR 083981A1 AR P110104381 A ARP110104381 A AR P110104381A AR P110104381 A ARP110104381 A AR P110104381A AR 083981 A1 AR083981 A1 AR 083981A1
Authority
AR
Argentina
Prior art keywords
cepa
inoculate
recombinant bacteria
application methods
nitrogen fixing
Prior art date
Application number
ARP110104381A
Other languages
English (en)
Inventor
Gabriela Cynthia Soto
Nicolas Daniel Ayub
Lorena Maria Setten
Original Assignee
Consejo Nac Invest Cient Tec
Inst Nac De Tecnologia Agropecuaria Inta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Consejo Nac Invest Cient Tec, Inst Nac De Tecnologia Agropecuaria Inta filed Critical Consejo Nac Invest Cient Tec
Priority to ARP110104381A priority Critical patent/AR083981A1/es
Priority to CN201280064329.3A priority patent/CN104136599B/zh
Priority to AU2012342047A priority patent/AU2012342047B2/en
Priority to PCT/IB2012/056643 priority patent/WO2013076687A2/en
Priority to BR112014012374A priority patent/BR112014012374A2/pt
Priority to US14/360,054 priority patent/US9657298B2/en
Publication of AR083981A1 publication Critical patent/AR083981A1/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60Isolated nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0095Oxidoreductases (1.) acting on iron-sulfur proteins as donor (1.18)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0097Oxidoreductases (1.) acting on reduced flavodoxin as donor (1.19)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • C12N1/205Bacterial isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38Pseudomonas
    • C12R2001/39Pseudomonas fluorescens

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cepa de bacterias recombinantes que comprende en su genoma genes nif heterólogos y es capaz de fijar nitrógeno. La cepa puede ser, por ejemplo, una cepa de Pseudomonas fluorescens recombinante que comprende en su genoma genes nif heterólogos. Se describe además un inóculo y un método para incrementar la productividad de las plantas.
ARP110104381A 2011-11-24 2011-11-24 Cepa de bacteria recombinante fijadora de nitrogeno, inoculo que la contiene y metodos de aplicacion AR083981A1 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ARP110104381A AR083981A1 (es) 2011-11-24 2011-11-24 Cepa de bacteria recombinante fijadora de nitrogeno, inoculo que la contiene y metodos de aplicacion
CN201280064329.3A CN104136599B (zh) 2011-11-24 2012-11-22 重组固氮细菌菌株、含有所述菌株的接种物以及应用方法
AU2012342047A AU2012342047B2 (en) 2011-11-24 2012-11-22 Recombinant nitrogen-fixing bacterial strain, inoculum containing the same and application methods
PCT/IB2012/056643 WO2013076687A2 (en) 2011-11-24 2012-11-22 Recombinant nitrogen-fixing bacterial strain, inoculum containing the same and application methods
BR112014012374A BR112014012374A2 (pt) 2011-11-24 2012-11-22 cepa bacteriana recombinante, inoculo para aumentar a produtividade de uma planta e método para aumentar a fixação de oxigênio em plantas
US14/360,054 US9657298B2 (en) 2011-11-24 2012-11-22 Recombinant nitrogen-fixing bacterial strain, inoculum containing the same and application methods

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP110104381A AR083981A1 (es) 2011-11-24 2011-11-24 Cepa de bacteria recombinante fijadora de nitrogeno, inoculo que la contiene y metodos de aplicacion

Publications (1)

Publication Number Publication Date
AR083981A1 true AR083981A1 (es) 2013-04-10

Family

ID=47557412

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110104381A AR083981A1 (es) 2011-11-24 2011-11-24 Cepa de bacteria recombinante fijadora de nitrogeno, inoculo que la contiene y metodos de aplicacion

Country Status (6)

Country Link
US (1) US9657298B2 (es)
CN (1) CN104136599B (es)
AR (1) AR083981A1 (es)
AU (1) AU2012342047B2 (es)
BR (1) BR112014012374A2 (es)
WO (1) WO2013076687A2 (es)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111018130A (zh) * 2019-12-11 2020-04-17 福建大北农水产科技有限公司 一种水产微生态制剂及其在降低养殖水体中氨氮和亚硝酸盐的应用

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2838955C (en) 2011-06-16 2023-10-24 The Regents Of The University Of California Synthetic gene clusters
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
EP3322679A4 (en) 2015-07-13 2019-07-10 Pivot Bio, Inc. METHOD AND COMPOSITIONS FOR IMPROVING PLANT PROPERTIES
AU2016336328A1 (en) * 2015-10-05 2018-04-19 Massachusetts Institute Of Technology Nitrogen fixation using refactored nif clusters
UY37566A (es) * 2017-01-12 2018-07-31 Pivot Bio Inc Métodos y composiciones para mejorar características en plantas
CN107119000B (zh) * 2017-04-19 2019-04-12 山东大学 荧光假单胞菌突变菌株的筛选方法及其在生物防治中的应用
BR112020008002A2 (pt) 2017-10-25 2020-11-03 Pivot Bio, Inc. alvos gênicos para fixação de nitrogênio para aprimorar traços de plantas
AU2018354221B2 (en) 2017-10-25 2025-04-17 Pivot Bio, Inc. Methods and compositions for improving engineered microbes that fix nitrogen
WO2020006064A2 (en) 2018-06-27 2020-01-02 Pivot Bio, Inc. Agricultural compositions comprising remodeled nitrogen fixing microbes
AU2019302747B2 (en) 2018-07-11 2025-11-20 Pivot Bio, Inc. Temporally and spatially targeted dynamic nitrogen delivery by remodeled microbes
CA3123940A1 (en) 2019-01-07 2020-07-16 Pivot Bio, Inc. Plant colonization assays using natural microbial barcodes
US12281299B2 (en) 2019-03-19 2025-04-22 Massachusetts Institute Of Technology Control of nitrogen fixation in rhizobia that associate with cereals
WO2021222643A1 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Stable liquid formulations for nitrogen-fixing microorganisms
EP4143293A1 (en) 2020-05-01 2023-03-08 Pivot Bio, Inc. Measurement of nitrogen fixation and incorporation
CN114410471B (zh) * 2021-12-24 2023-11-24 浙江大学 水稻根际土壤非共生固氮菌株的筛选方法及所用培养基

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0108508B1 (en) 1982-10-08 1990-02-07 State Of Oregon By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Hydrogen uptake gene cosmid and process of constructing same
US6277632B1 (en) * 1996-06-17 2001-08-21 Vectorobjects, Llc Method and kits for preparing multicomponent nucleic acid constructs
CN101319198B (zh) * 2008-06-23 2010-04-14 中国科学院南海海洋研究所 红树植物根际促生固氮菌(dzy-n56)及其应用
CN102041241A (zh) 2009-10-20 2011-05-04 中国农业科学院生物技术研究所 高效泌铵的联合固氮菌株

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111018130A (zh) * 2019-12-11 2020-04-17 福建大北农水产科技有限公司 一种水产微生态制剂及其在降低养殖水体中氨氮和亚硝酸盐的应用

Also Published As

Publication number Publication date
WO2013076687A2 (en) 2013-05-30
WO2013076687A3 (en) 2013-07-18
AU2012342047B2 (en) 2016-04-14
CN104136599B (zh) 2017-05-24
AU2012342047A1 (en) 2014-06-12
US20140336050A1 (en) 2014-11-13
CN104136599A (zh) 2014-11-05
US9657298B2 (en) 2017-05-23
BR112014012374A2 (pt) 2019-03-26

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