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AR081386A1 - Composiciones, organismos, sistemas y metodos para expresar un producto genetico en plantas - Google Patents

Composiciones, organismos, sistemas y metodos para expresar un producto genetico en plantas

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Publication number
AR081386A1
AR081386A1 ARP110101620A ARP110101620A AR081386A1 AR 081386 A1 AR081386 A1 AR 081386A1 AR P110101620 A ARP110101620 A AR P110101620A AR P110101620 A ARP110101620 A AR P110101620A AR 081386 A1 AR081386 A1 AR 081386A1
Authority
AR
Argentina
Prior art keywords
exna
ecs
plant
expression
nucleic acid
Prior art date
Application number
ARP110101620A
Other languages
English (en)
Inventor
T Erik Mirkov
Jong Won Park
san-ji Gao
Getu B Diguma
Original Assignee
Texas A & M Univ Sys
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Texas A & M Univ Sys filed Critical Texas A & M Univ Sys
Publication of AR081386A1 publication Critical patent/AR081386A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/34—Vector systems having a special element relevant for transcription being a transcription initiation element

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Composiciones, organismos, sistemas y métodos para expresar un producto genético en una planta usando una secuencia de control de la expresion (ECS) funcional en monocotiledoneas y/o dicotiledoneas. Por ejemplo, (i) un ácido nucleico aislado puede comprender una ECS (por ejemplo, un promotor virus baciliforme de la cana de azucar) y, opcionalmente, un ácido nucleico exogeno (ExNA) ligado operativamente a la ECS; (u) un vector de expresion puede comprender una ECS; un ExNA; y, opcionalmente, una secuencia de terminacion 3 , en donde la ECS tiene suficiente actividad promotora como para expresar el ExNA en al menos una monocotiledonea y al menos una dicotiledonea; (iii) un microorganismo, célula vegetal o planta puede comprender un ácido nucleico aislado; (iv) un método para expresar constitutivamente un ExNA en una planta (por ejemplo, una monocotiledonea y/o una dicotiledonea) que puede comprender el contacto de un vector de expresion con el citosol de una célula de la planta, en donde dicho vector de expresion comprende al ExNA y una ECS funcional para dirigir la expresion del ExNA; y/o (y) un método para dirigir la expresion constitutiva de un ácido nucleico en una planta (por ejemplo, una monocotiledonea y/o una dicotiledonea) que puede comprender transformar la planta con un ácido nucleico de expresion que comprende una ECS, un ExNA y una secuencia de terminacion 3'.
ARP110101620A 2010-05-10 2011-05-10 Composiciones, organismos, sistemas y metodos para expresar un producto genetico en plantas AR081386A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39519710P 2010-05-10 2010-05-10
US40097610P 2010-08-05 2010-08-05

Publications (1)

Publication Number Publication Date
AR081386A1 true AR081386A1 (es) 2012-08-29

Family

ID=44352076

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110101620A AR081386A1 (es) 2010-05-10 2011-05-10 Composiciones, organismos, sistemas y metodos para expresar un producto genetico en plantas

Country Status (6)

Country Link
US (2) US8710207B2 (es)
CN (2) CN107090453A (es)
AR (1) AR081386A1 (es)
AU (2) AU2011252008B2 (es)
BR (1) BR112012028848A2 (es)
WO (1) WO2011143204A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA020327B1 (ru) * 2008-06-25 2014-10-30 Атеникс Корпорейшн Гены токсинов и способы их применения
EP2797407A4 (en) * 2011-12-30 2015-07-08 Dow Agrosciences Llc METHOD AND CONSTRUCTION FOR SYNTHETIC BIDIRECTIONAL SCBV PLANT PROMOTER
US20130205443A1 (en) 2012-01-27 2013-08-08 The Texas A&M University System Pathogen resistant citrus compositions, organisms, systems, and methods
AR090204A1 (es) * 2012-02-29 2014-10-29 Dow Agrosciences Llc Potenciador viral baciliforme de caña de azucar (scbv) y su uso en la genomica funcional de plantas
BR112016020879A2 (pt) * 2014-03-10 2018-01-23 Texas A & M Univ Sys composições, organismos, sistemas e métodos para expressar um produto gênico em plantas
TWI530503B (zh) * 2014-07-21 2016-04-21 元智大學 修飾的啟動子序列及其應用
CN109402155A (zh) * 2018-11-12 2019-03-01 川北医学院 一种双控延迟裂解性质粒及其构建方法与应用
WO2021026689A1 (zh) * 2019-08-09 2021-02-18 北京大北农生物技术有限公司 用于检测大豆植物dbn8007的核酸序列及其检测方法
CN110452905B (zh) * 2019-08-22 2023-06-02 云南省烟草农业科学研究院 一种提高烟草dna沉淀效率的提取方法及其应用
CN114574492B (zh) * 2022-03-25 2023-09-19 广东省科学院南繁种业研究所 一种来自甘蔗杆状病毒的组成型启动子pscbv-chn1及其应用
CN114591981B (zh) * 2022-03-25 2023-05-26 江苏师范大学 Tppi基因在调控植物根系发育和促进植物茉莉酸积累方面的应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5994123A (en) 1996-08-09 1999-11-30 Regents Of University Of Minnesota Sugarcane bacilliform virus promoter
CA2323726C (en) * 1998-03-20 2005-02-08 Benitec Australia Ltd. Control of gene expression
US6479636B1 (en) 2000-04-11 2002-11-12 Honiron Corporation (A Louisiana Corporation) Sugarcane fractioning system
US20020042928A1 (en) * 2000-07-11 2002-04-11 Mccaslin Mark H. Methods for maximizing expression of transgenic traits in autopolyploid plants
AUPR160400A0 (en) * 2000-11-21 2000-12-14 Bureau Of Sugar Experiment Stations Plant virus promoter
US7001739B2 (en) 2001-08-24 2006-02-21 The Texas A&M University System Isolation of proteins involved in posttranscriptional gene silencing and methods of use
US20040040061A1 (en) * 2002-02-27 2004-02-26 Michael Horn Expression in plants of HIV-related proteins
EP1590447B1 (en) * 2003-01-03 2012-03-14 The Texas A & M University System Stem-regulated, plant defense promoter and uses thereof in tissue-specific expression in monocots

Also Published As

Publication number Publication date
BR112012028848A2 (pt) 2018-05-15
US20140208462A1 (en) 2014-07-24
AU2016203951B2 (en) 2018-12-06
AU2011252008B2 (en) 2016-03-17
US10202612B2 (en) 2019-02-12
CN103052712A (zh) 2013-04-17
CN103052712B (zh) 2016-10-12
CN107090453A (zh) 2017-08-25
US8710207B2 (en) 2014-04-29
WO2011143204A2 (en) 2011-11-17
WO2011143204A3 (en) 2012-01-05
US20110283377A1 (en) 2011-11-17
AU2011252008A1 (en) 2012-11-29
AU2016203951A1 (en) 2016-07-07

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