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AR084630A1 - Metodos para determinar la zigosidad en una muestra compuesta - Google Patents

Metodos para determinar la zigosidad en una muestra compuesta

Info

Publication number
AR084630A1
AR084630A1 ARP110104981A ARP110104981A AR084630A1 AR 084630 A1 AR084630 A1 AR 084630A1 AR P110104981 A ARP110104981 A AR P110104981A AR P110104981 A ARP110104981 A AR P110104981A AR 084630 A1 AR084630 A1 AR 084630A1
Authority
AR
Argentina
Prior art keywords
nucleic acid
methods
nucleotide sequence
zigosity
primers
Prior art date
Application number
ARP110104981A
Other languages
English (en)
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of AR084630A1 publication Critical patent/AR084630A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Los métodos para determinar la presencia o la ausencia de una secuencia de nucleótidos insertada en un sitio de inserción particular de un ácido nucleico incluyen: aislar ácido nucleico a partir de la muestra de tejidos compuesta; poner el ácido nucleico en contacto con un cebador directo capaz de ligarse al ácido nucleico corriente arriba del sitio de inserción, un primer cebador inverso específico a la secuencia de nucleótidos insertada y un segundo cebador inverso capaz de ligarse al ácido nucleico corriente abajo del sitio de inserción. Se pueden usar los cebadores para reproducir ácidos nucleicos entre los cebadores. Se pueden analizar los ácidos nucleicos reproducidos para determinar si una secuencia de nucleótidos insertada está presente o ausente en la muestra.
ARP110104981A 2010-12-29 2011-12-28 Metodos para determinar la zigosidad en una muestra compuesta AR084630A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201061428142P 2010-12-29 2010-12-29

Publications (1)

Publication Number Publication Date
AR084630A1 true AR084630A1 (es) 2013-05-29

Family

ID=46383837

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP110104981A AR084630A1 (es) 2010-12-29 2011-12-28 Metodos para determinar la zigosidad en una muestra compuesta

Country Status (16)

Country Link
US (1) US20140017684A1 (es)
EP (1) EP2659006A4 (es)
CN (1) CN103403185A (es)
AR (1) AR084630A1 (es)
AU (2) AU2011352159A1 (es)
BR (1) BRPI1105703A2 (es)
CA (1) CA2822967A1 (es)
CL (1) CL2013001893A1 (es)
CO (1) CO6731137A2 (es)
MX (1) MX2013007573A (es)
NZ (1) NZ611916A (es)
RU (2) RU2605324C2 (es)
UA (1) UA112977C2 (es)
UY (1) UY33843A (es)
WO (1) WO2012092327A2 (es)
ZA (1) ZA201304475B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018100466A1 (en) * 2016-11-30 2018-06-07 Semiconductor Energy Laboratory Co., Ltd. Display device, display module, and electronic device
BR112021012816A2 (pt) * 2019-03-28 2021-11-23 Bioceres Llc Evento transgênico ind-øø41ø-5 da soja
EP4644572A1 (en) * 2024-04-29 2025-11-05 KWS SAAT SE & Co. KGaA Method for analysing trait purity in large seed bulks

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428147A (en) * 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US6346655B1 (en) * 1999-03-31 2002-02-12 Syngenta Participations Ag Trichothecne-Resistant transgenic plants
FR2796963B1 (fr) * 1999-07-28 2001-09-28 Rhobio Procede d'obtention de lignees isotransgeniques
DK1620571T3 (en) * 2003-05-02 2015-10-05 Dow Agrosciences Llc MAJS TD1507 and methods for detecting it
US8212113B2 (en) * 2003-12-15 2012-07-03 Monsanto Technology Llc Corn plant Mon88017 and compositions and methods for detection thereof
BRPI0515922B8 (pt) * 2004-09-29 2022-12-06 Dow Agrosciences Llc Molécula de dna, kit, constructo, método de identificação do evento, método de detecção, par de moléculas de dna, método de confirmação da pureza, método de varredura, par de sequências de dna
PT1885176T (pt) * 2005-05-27 2016-11-28 Monsanto Technology Llc Evento mon89788 de soja e métodos para a sua deteção
CN101278053A (zh) * 2005-07-29 2008-10-01 孟山都技术有限公司 利用来自转基因杂交体的分离子来开发新的种质
MX2008001839A (es) * 2005-08-08 2008-04-09 Bayer Bioscience Nv Plantas de algodon con tolerancia a herbicidas y metodos para identificar las mismas.
AU2007227665A1 (en) * 2006-03-17 2007-09-27 Merck & Co., Inc. Method for genetic selection of high-plasmid producing E. coli clones
CN1873010B (zh) * 2006-04-14 2010-04-07 中国科学院武汉植物园 利用花生Ara h3启动子转基因载体的制备方法及应用
US7928295B2 (en) * 2006-08-24 2011-04-19 Bayer Bioscience N.V. Herbicide tolerant rice plants and methods for identifying same
WO2008122406A1 (en) * 2007-04-05 2008-10-16 Bayer Bioscience N.V. Insect resistant cotton plants and methods for identifying same
US8999634B2 (en) * 2007-04-27 2015-04-07 Quest Diagnostics Investments Incorporated Nucleic acid detection combining amplification with fragmentation
EP2162542A1 (en) * 2007-06-11 2010-03-17 Bayer BioScience N.V. Insect resistant cotton plants comprising elite event ee-gh6 and methods for identifying same
PL2220239T3 (pl) * 2007-11-28 2015-10-30 Bayer Cropscience Nv Roślina Brassica zawierająca zmutowane allele typu indehiscent
US8097412B2 (en) * 2008-07-12 2012-01-17 Biodiagnostics, Inc. DNA-based test for detection of annual and intermediate ryegrass

Also Published As

Publication number Publication date
UY33843A (es) 2012-07-31
RU2605324C2 (ru) 2016-12-20
MX2013007573A (es) 2013-07-22
EP2659006A2 (en) 2013-11-06
CA2822967A1 (en) 2012-07-05
RU2016144372A (ru) 2018-12-18
WO2012092327A2 (en) 2012-07-05
WO2012092327A3 (en) 2013-01-10
US20140017684A1 (en) 2014-01-16
CN103403185A (zh) 2013-11-20
NZ611916A (en) 2015-12-24
RU2013135397A (ru) 2015-02-10
UA112977C2 (uk) 2016-11-25
BRPI1105703A2 (pt) 2015-08-04
ZA201304475B (en) 2014-09-25
EP2659006A4 (en) 2014-10-29
CL2013001893A1 (es) 2013-12-06
CO6731137A2 (es) 2013-08-15
AU2011352159A1 (en) 2013-07-04
AU2016262648A1 (en) 2016-12-08

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