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AR079813A1 - Metodos para controlar el nematodo de las lesiones de las raices - Google Patents

Metodos para controlar el nematodo de las lesiones de las raices

Info

Publication number
AR079813A1
AR079813A1 ARP100105017A ARP100105017A AR079813A1 AR 079813 A1 AR079813 A1 AR 079813A1 AR P100105017 A ARP100105017 A AR P100105017A AR P100105017 A ARP100105017 A AR P100105017A AR 079813 A1 AR079813 A1 AR 079813A1
Authority
AR
Argentina
Prior art keywords
nematodes
seq
white
plant
nucleic acid
Prior art date
Application number
ARP100105017A
Other languages
English (en)
Inventor
Carl Diehl
James P Mccarter
Brandi Chiapelli
Matt W Dimmic
Jeremy Williams
Jennifer Sheppard
John D Bradley
Evan James Diehl
Michelle L Gasper
Bingli Gao
Original Assignee
Divergence Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Divergence Inc filed Critical Divergence Inc
Publication of AR079813A1 publication Critical patent/AR079813A1/es

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering nucleic acids [NA]
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Genes blancos, construcciones y métodos para efectuar el control genético de una enfermedad en una planta provocada por nematodos del género Pratylenchus (los nematodos de las lesiones de las raíces). La presente se relaciona con la obtencion de un efecto de proteccion en la planta a través de la identificacion de secuencias codificantes blanco y el uso de tecnología de ADN recombinante para la represion o la inhibicion posterior a la transcripcion de la expresion de las secuencias codificantes blanco en las células de los nematodos parásitos de las plantas. Los genes blanco descriptos están conservados, y se espera que sean esenciales y sensibles a la perturbacion por ARNi en distintas especies de Pratylenchus, lo que facilitará un abordaje basado en la interferencia a nivel del ARN en todo el género. Reivindicacion 21: Un método para controlar una poblacion de nematodos parásitos de las plantas caracterizado porque comprende proveer un agente que comprende una molécula de ribonucleotido de cadena doble que, al ser captada por los nematodos, inhibe una funcion biologica en ellos, donde el agente comprende (a) una secuencia de nucleotidos que comprende al menos 17, 18, 19, 20 o 21 nucleotidos contiguos de una secuencia de ácido nucleico de cualquiera de SEQ ID N° 1-51 o SEQ ID N° 120-1064; y (b) el complemento inverso de la secuencia de nucleotidos que comprende al menos 17, 18, 19, 20 o 21 nucleotidos contiguos de una secuencia de ácido nucleico de cualquiera de SEQ ID N° 1-51 o SEQ ID N° 120-1064.
ARP100105017A 2009-12-30 2010-12-29 Metodos para controlar el nematodo de las lesiones de las raices AR079813A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US29106409P 2009-12-30 2009-12-30

Publications (1)

Publication Number Publication Date
AR079813A1 true AR079813A1 (es) 2012-02-22

Family

ID=44227142

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP100105017A AR079813A1 (es) 2009-12-30 2010-12-29 Metodos para controlar el nematodo de las lesiones de las raices

Country Status (4)

Country Link
US (2) US8907162B2 (es)
AR (1) AR079813A1 (es)
BR (1) BR112012016197A2 (es)
WO (1) WO2011082217A2 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8907162B2 (en) * 2009-12-30 2014-12-09 Monsanto Technology Llc Compositions and methods for the control of root lesion nematode
WO2014090765A1 (en) 2012-12-12 2014-06-19 Bayer Cropscience Ag Use of 1-[2-fluoro-4-methyl-5-(2,2,2-trifluoroethylsulfinyl)phenyl]-5-amino-3-trifluoromethyl)-1 h-1,2,4 tfia zole for controlling nematodes in nematode-resistant crops
US10308954B2 (en) * 2012-12-14 2019-06-04 Icar Method for the control of nematodes in plants
WO2014198729A1 (en) * 2013-06-10 2014-12-18 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and compositions for controlling nematodes
WO2016018887A1 (en) * 2014-07-29 2016-02-04 Monsanto Technology Llc Compositions and methods for controlling insect pests
CN107849568B (zh) 2015-08-07 2021-10-26 拜尔作物科学公司 根优先和胁迫诱导型启动子及其用途
CN109456975B (zh) * 2016-01-04 2021-04-30 北京大北农生物技术有限公司 用于控制昆虫侵袭的核苷酸序列及其方法
WO2021092251A1 (en) 2019-11-05 2021-05-14 Apeel Technology, Inc. Prediction of infection in plant products

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR0312580A (pt) * 2002-07-10 2006-10-10 Univ Kansas State composições e métodos para controlar nematódeos parasitas
US20040185566A1 (en) 2003-03-19 2004-09-23 Peter Salamone Polypeptides toxic to nematodes and methods of use
US8088976B2 (en) 2005-02-24 2012-01-03 Monsanto Technology Llc Methods for genetic control of plant pest infestation and compositions thereof
AR059433A1 (es) 2006-02-10 2008-04-09 Monsanto Technology Llc Identificacion y uso de genes blanco para el control de nematodos parasitos de plantas
US20100068172A1 (en) * 2006-03-16 2010-03-18 Devgen N.V. Nematode Control
CN101679995A (zh) 2007-03-15 2010-03-24 巴斯福植物科学有限公司 线虫几丁质酶基因用于控制植物寄生性线虫的用途
US8299318B2 (en) * 2007-07-05 2012-10-30 Ceres, Inc. Nucleotide sequences and corresponding polypeptides conferring modulated plant characteristics
US8901373B2 (en) 2008-04-10 2014-12-02 Monsanto Technology Llc Methods and compositions for root knot nematode control
US8907162B2 (en) * 2009-12-30 2014-12-09 Monsanto Technology Llc Compositions and methods for the control of root lesion nematode

Also Published As

Publication number Publication date
BR112012016197A2 (pt) 2015-09-01
US20130036519A1 (en) 2013-02-07
WO2011082217A3 (en) 2012-01-19
WO2011082217A2 (en) 2011-07-07
US9790516B2 (en) 2017-10-17
US8907162B2 (en) 2014-12-09
US20150135363A1 (en) 2015-05-14

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