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AR078849A1 - Celulas madre multipotenciales del arbol biliar extrahepatico y metodos de aislamiento de las mismas - Google Patents

Celulas madre multipotenciales del arbol biliar extrahepatico y metodos de aislamiento de las mismas

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Publication number
AR078849A1
AR078849A1 ARP100104019A ARP100104019A AR078849A1 AR 078849 A1 AR078849 A1 AR 078849A1 AR P100104019 A ARP100104019 A AR P100104019A AR P100104019 A ARP100104019 A AR P100104019A AR 078849 A1 AR078849 A1 AR 078849A1
Authority
AR
Argentina
Prior art keywords
multipotential
cells
cell
stem
pancreatic
Prior art date
Application number
ARP100104019A
Other languages
English (en)
Inventor
Lola M Reid
Yunfang Wang
Vicenzo Cardinale
Eugenio Gaudio
Guido Carpino
Domenico Alvaro
Cai-Bin Cui
Original Assignee
Univ North Carolina
Sapienza University In Rome
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43922533&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR078849(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ North Carolina, Sapienza University In Rome filed Critical Univ North Carolina
Publication of AR078849A1 publication Critical patent/AR078849A1/es

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
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    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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Abstract

Una célula madre multipotencial, poblaciones de células multipotenciales y una poblacion de células multipotenciales enriquecida, cada una presente en el tejido biliar fetal, neonatal, pediátrico y de adultos y hasta 72 horas de post-mortem (aunque con preferencia dentro de las 10 horas de post-mortem) y con capacidad de madurar en multiples tejidos endodérmicos que incluyen los tejidos hepáticos, biliares y pancreáticos. La célula madre/progenitora multipotencial y las poblaciones de células se encuentran en las glándulas peribiliares, y la progenie descendiente de las mismas está presente en todo el árbol biliar, inclusive en la vesícula biliar. Existe una gran cantidad de glándulas peribiliares en las ubicaciones ramificadas del árbol biliar tal como el hilio, el dueto hepático comun, el dueto cístico, el dueto comun, el dueto hepato-pancreático comun y la vesícula biliar. Hay células multipotenciales relacionadas, poblaciones de células multipotenciales y sus progenitores descendientes en todo el árbol biliar, inclusive en la vesícula biliar, que no tiene glándulas peri biliares. Se proveen métodos para su identificacion y aislamiento, mantenimiento de las mismas en cultivo, su expansion y diferenciacion en cultivo o restriccion de linaje de las mismas in vitro o in vivo al destino hepático, biliar o pancreático (por ejemplo, como hepatocitos, colangiocitos y/o células de los islotes pancreáticos). También se proveen métodos de uso de las células multipotenciales y/o poblaciones de células multipotenciales. Reivindicacion 1: Una composicion, caracterizada porque comprende células madre/progenitoras multipotenciales de mamífero capaces de diferenciarse en multiples linajes endodérmicos, en donde las células se obtienen de tejido del árbol biliar de un mamífero. Reivindicacion 10: La composicion de la reivindicacion 1, caracterizada porque las células multipotenciales expresan: (i) al menos un marcador indicativo de linajes de células hepáticas en etapa temprana; (ii) al menos un marcador indicativo de linajes de células pancreáticas en etapa temprana; y al menos un marcador seleccionado entre las categorías (a) - (c): (a) al menos un marcador de superficie presente sobre las células madre/progenitoras; (b) al menos un factor de transcripcion indicativo de células madre/progenitoras endodérmicas; y (c) expresion débil a moderada de un gen pluripotencial.
ARP100104019A 2009-10-30 2010-10-29 Celulas madre multipotenciales del arbol biliar extrahepatico y metodos de aislamiento de las mismas AR078849A1 (es)

Applications Claiming Priority (1)

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US25684609P 2009-10-30 2009-10-30

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AR078849A1 true AR078849A1 (es) 2011-12-07

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ID=43922533

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ARP100104019A AR078849A1 (es) 2009-10-30 2010-10-29 Celulas madre multipotenciales del arbol biliar extrahepatico y metodos de aislamiento de las mismas
ARP200100301A AR122249A2 (es) 2009-10-30 2020-02-04 Célula madre multipotencial del árbol biliar extrahepático de mamífero y composición que la comprende

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US (1) US20110135610A1 (es)
EP (2) EP3725874A1 (es)
JP (2) JP5936547B2 (es)
KR (4) KR20120117758A (es)
CN (2) CN102918145B (es)
AR (2) AR078849A1 (es)
AU (1) AU2016210783B2 (es)
BR (1) BR112012009848B1 (es)
CA (2) CA2779144C (es)
CL (1) CL2012001117A1 (es)
CR (1) CR20120254A (es)
ES (1) ES2819028T3 (es)
IL (2) IL219454A (es)
MX (1) MX2012005087A (es)
NZ (3) NZ622427A (es)
PH (1) PH12012500845A1 (es)
RU (2) RU2756584C2 (es)
SG (2) SG10201913006XA (es)
TW (3) TWI686478B (es)
WO (1) WO2011053690A1 (es)

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IL311366B2 (en) * 2018-03-29 2025-05-01 Univ North Carolina Chapel Hill Stem/progenitor cells from duodenal Brunner's glands and methods for isolating and using them
CN112126618B (zh) * 2019-06-24 2023-09-12 上海拜羡生物科技有限公司 一种人胆囊干细胞的获取和长期体外培养的方法
CN113388569B (zh) * 2020-08-28 2022-05-17 广东乾晖生物科技有限公司 肝脏类器官的制备方法
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