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AR051895A1 - Metodo para disparar la interferencia de arn - Google Patents

Metodo para disparar la interferencia de arn

Info

Publication number
AR051895A1
AR051895A1 ARP060100064A ARP060100064A AR051895A1 AR 051895 A1 AR051895 A1 AR 051895A1 AR P060100064 A ARP060100064 A AR P060100064A AR P060100064 A ARP060100064 A AR P060100064A AR 051895 A1 AR051895 A1 AR 051895A1
Authority
AR
Argentina
Prior art keywords
gene
cell
sirna
white
plant cell
Prior art date
Application number
ARP060100064A
Other languages
English (en)
Inventor
James C Carrington
Edwards Allen
Original Assignee
Ard Of Higher Education On Beh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ard Of Higher Education On Beh filed Critical Ard Of Higher Education On Beh
Publication of AR051895A1 publication Critical patent/AR051895A1/es

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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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Abstract

Un cassette de ácido nucleico que dispara siARN o ARNi, que comprende: una secuencia de iniciador que comprende de 20 a 25 nucleotidos, y que tiene un sitio de ruptura de iniciacion alrededor del décimo y undécimo nucleotido contado desde el extremo de 3' de la secuencia de iniciador; y al menos un segmento supresor de genes en registro de 21 nucleotidos contados hacia arriba desde el sitio de ruptura de iniciacion, en donde el segmento supresor de genes o su complemento es sustancialmente complementario de mARN transcripto desde un gen de blanco seleccionado para la inhibicion de siARN. Vector de expresion que comprende dicho cassette de ácido nucleico. Célula de planta transgénica que expresaran para suprimir un gen blanco, en donde la célula de planta comprende ADN recombinante desde el cual se transcribe un primer ARN. Método para inhibir la expresion de un gen blanco o una secuencia en una célula. Método de inducir la produccion de al menos un siARN en una célula. Método de inhibir la actividad de un gen blanco en una célula de planta. Método de inhibir la actividad de un gen blanco en una célula de semilla de planta.
ARP060100064A 2005-01-07 2006-01-06 Metodo para disparar la interferencia de arn AR051895A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US64212605P 2005-01-07 2005-01-07

Publications (1)

Publication Number Publication Date
AR051895A1 true AR051895A1 (es) 2007-02-14

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Application Number Title Priority Date Filing Date
ARP060100064A AR051895A1 (es) 2005-01-07 2006-01-06 Metodo para disparar la interferencia de arn

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Country Link
US (7) US8030473B2 (es)
EP (1) EP1838144B1 (es)
AR (1) AR051895A1 (es)
AU (1) AU2006203892B2 (es)
CA (1) CA2593238C (es)
WO (1) WO2006074400A2 (es)

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Publication number Priority date Publication date Assignee Title
US7612194B2 (en) * 2001-07-24 2009-11-03 Monsanto Technology Llc Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof
EP1818405B2 (en) 2004-04-09 2025-03-19 Monsanto Technology, LLC Compositions and methods for control of insect infestations in plants
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
EP1838144B1 (en) * 2005-01-07 2016-08-31 Oregon State University Method to trigger rna interference
CN101203611B (zh) * 2005-04-19 2013-08-14 巴斯福植物科学有限公司 控制基因表达的改良方法
CA2612252A1 (en) * 2005-06-17 2006-12-28 Pioneer Hi-Bred International, Inc. Methods and compositions for gene silencing
EP2439279B1 (en) 2005-09-16 2016-11-09 Monsanto Technology LLC Methods for genetic control of insect infestations in plants and compositions thereof
CN101268194A (zh) * 2005-09-20 2008-09-17 巴斯福植物科学有限公司 使用ta-siRNA调控基因表达的方法
WO2007047016A2 (en) 2005-10-13 2007-04-26 Monsanto Technology, Llc Methods for producing hybrid seed
WO2008063203A2 (en) * 2006-01-27 2008-05-29 Whitehead Institute For Biomedical Research Compositions and methods for efficient gene silencing in plants
US20070214521A1 (en) * 2006-03-08 2007-09-13 The Regents Of The University Of California Role of microRNA in plant salt tolerance
LT2056845T (lt) 2006-08-08 2017-11-27 Rheinische Friedrich-Wilhelms-Universität Bonn 5` fosfato oligonukleotidų struktūra ir panaudojimas
ES2655700T3 (es) * 2006-08-31 2018-02-21 Monsanto Technology, Llc ARN pequeños en fase
US8946511B2 (en) 2006-10-12 2015-02-03 Monsanto Technology Llc Plant microRNAs and methods of use thereof
CN101195821A (zh) * 2006-12-04 2008-06-11 中国科学院上海生命科学研究院 利用RNAi技术改良植物抗虫性的方法
EP2115141A4 (en) * 2007-02-20 2010-08-04 Monsanto Technology Llc miRNA FROM INVERTEBRATES
US8097712B2 (en) 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
US8115055B2 (en) 2007-12-18 2012-02-14 E.I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
EP2229448A2 (en) * 2007-12-21 2010-09-22 E. I. du Pont de Nemours and Company Drought tolerant plants and related constructs and methods involving genes encoding mir827
EP2297323A1 (en) 2008-05-21 2011-03-23 Hartmann, Gunther 5' triphosphate oligonucleotide with blunt end and uses thereof
US20110184045A1 (en) * 2008-06-30 2011-07-28 Gunther Hartmann Silencng and rig-i activation by dual function oligonucleotides
US8435790B2 (en) * 2008-07-25 2013-05-07 The Regents Of The University Of California Methods of modulating lipid concentrations in eukaryotic cells
MA33281B1 (fr) 2009-04-20 2012-05-02 Monsanto Technology Llc Résistance à de multiples virus dans des plantes
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
WO2011067745A2 (en) * 2009-12-06 2011-06-09 Rosetta Green Ltd. Compositions and methods for enhancing plants resistance to abiotic stress
MX2012010479A (es) 2010-03-08 2012-10-09 Monsanto Technology Llc Moleculas polinucleotidicas para regulacion genetica en plantas.
WO2011146527A2 (en) * 2010-05-17 2011-11-24 Zirus, Inc. Mammalian genes involved in infection
US9222100B2 (en) 2010-08-24 2015-12-29 Monsanto Technology Llc Methods and DNA constructs for autoregulating transgene silencing
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
WO2012149316A2 (en) * 2011-04-28 2012-11-01 Iowa State University Research Foundation, Inc. miRNA396 AND GROWTH REGULATING FACTORS FOR CYST NEMATODE TOLERANCE IN PLANTS
US8841272B2 (en) * 2011-05-31 2014-09-23 Kansas State University Research Foundation Double-stranded RNA-based nanoparticles for insect gene silencing
MX354471B (es) 2011-07-01 2018-03-07 Monsanto Technology Llc Métodos y composiciones para la regulación selectiva de la expresión de proteínas.
US9181532B2 (en) 2011-07-29 2015-11-10 Icon Genetics Gmbh Production of galactosylated N-glycans in plants
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
CN110066794A (zh) 2011-09-13 2019-07-30 孟山都技术公司 用于杂草控制的方法和组合物
EP3296402B1 (en) 2011-09-13 2020-04-15 Monsanto Technology LLC Methods and compositions for weed control
MX362810B (es) 2011-09-13 2019-02-13 Monsanto Technology Llc Metodos y composiciones para controlar malezas.
MX362812B (es) 2011-09-13 2019-02-13 Monsanto Technology Llc Metodos y composiciones para el control de malezas.
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
WO2013053070A1 (en) * 2011-10-09 2013-04-18 Shanghai Institutes For Biological Sciences, Cas Heat tolerance microrna
JP6104252B2 (ja) 2011-10-04 2017-03-29 アイコン・ジェネティクス・ゲーエムベーハー フコシルトランスフェラーゼ活性が欠損したニコチアナ・ベンサミアナ植物
WO2013175480A1 (en) * 2012-05-24 2013-11-28 A.B. Seeds Ltd. Compositions and methods for silencing gene expression
CA2878286C (en) 2012-07-06 2023-06-13 Bayer Cropscience Nv Brassica rod1 gene sequences and uses thereof
JP2015524251A (ja) 2012-07-06 2015-08-24 バイエル・クロップサイエンス・エヌ・ヴェーＢａｙｅｒ Ｃｒｏｐｓｃｉｅｎｃｅ Ｎ．Ｖ． 種子油組成が改良されたアブラナ属植物
CA2878287A1 (en) 2012-07-06 2014-01-09 Bayer Cropscience Nv Soybean rod1 gene sequences and uses thereof
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
CA2888264A1 (en) 2012-10-18 2014-04-24 Monsanto Technology Llc Methods and compositions for plant pest control
US20150307894A1 (en) 2012-11-28 2015-10-29 Monsanto Technology Llc Transgenic Plants With Enhanced Traits
MX375327B (es) 2013-01-01 2025-03-06 A B Seeds Ltd Métodos para introducir dsrna en semillas de plantas para modular la expresión genética.
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
US20160024518A1 (en) * 2013-03-13 2016-01-28 Brian McGonigle Production of small interfering rnas in planta
CN105074008A (zh) 2013-03-13 2015-11-18 孟山都技术有限公司 用于杂草控制的方法和组合物
WO2014164797A2 (en) 2013-03-13 2014-10-09 Monsanto Technology Llc Methods and compositions for weed control
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
EP3017049B1 (en) 2013-07-01 2018-08-22 Bayer CropScience NV Methods and means for modulating flowering time in monocot plants
CA2918387C (en) 2013-07-19 2021-11-02 Monsanto Technology Llc Compositions and methods for controlling leptinotarsa
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
BR112016005859A2 (pt) 2013-09-24 2017-09-19 Basf Se Heterotransglicosilase e usos da mesma
NZ719544A (en) 2013-11-04 2022-09-30 Beeologics Inc Compositions and methods for controlling arthropod parasite and pest infestations
EP2873735A1 (en) * 2013-11-14 2015-05-20 Universität Potsdam Method and materials for making plants heat-resistant
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
US10683513B2 (en) * 2013-12-31 2020-06-16 Dow Agrosciences Llc Tissue-specific expression and hybrid plant production
UA121462C2 (uk) 2014-01-15 2020-06-10 Монсанто Текнолоджі Елелсі Спосіб та композиція для боротьби із бур'янами з використанням полінуклеотидів epsps
EP3420809A1 (en) 2014-04-01 2019-01-02 Monsanto Technology LLC Compositions and methods for controlling insect pests
EP3158067B1 (en) 2014-06-23 2020-08-12 Monsanto Technology LLC Compositions and methods for regulating gene expression via rna interference
EP3161138A4 (en) 2014-06-25 2017-12-06 Monsanto Technology LLC Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
WO2016018887A1 (en) 2014-07-29 2016-02-04 Monsanto Technology Llc Compositions and methods for controlling insect pests
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
EP3207827B1 (en) 2014-10-17 2020-07-15 Goodbaby Child Products Co., Ltd Travel bed
WO2016113333A1 (en) 2015-01-16 2016-07-21 Bayer Cropscience Nv Leaf-preferential promoters and uses thereof
CA2974101A1 (en) 2015-01-22 2016-07-28 Monsanto Technology Llc Compositions and methods for controlling leptinotarsa
WO2016128519A1 (en) 2015-02-12 2016-08-18 Bayer Cropscience Nv Shoot apex-preferential promoters and uses thereof
WO2016196738A1 (en) 2015-06-02 2016-12-08 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
CN108024517A (zh) 2015-06-03 2018-05-11 孟山都技术公司 用于将核酸引入到植物中的方法和组合物
CN107849568B (zh) 2015-08-07 2021-10-26 拜尔作物科学公司 根优先和胁迫诱导型启动子及其用途
CA3005985C (en) * 2015-09-09 2025-04-22 Psomagen, Inc. METHOD AND SYSTEM FOR MICROBIOME-DERIVED DIAGNOSTICS AND THERAPEUTIC AGENTS FOR INFECTIOUS DISEASE OR OTHER HEALTH CONDITIONS ASSOCIATED WITH ANTIBIOTIC USE
CA3017594A1 (en) 2016-03-15 2017-09-21 National Research Council Of Canada Modulating plant abiotic stress responses using the kanghan gene family
US11859196B2 (en) 2016-03-15 2024-01-02 National Research Council Of Canada Modulating drought tolerance in Brassicaceae using the Kanghan gene family
IL262056B2 (en) * 2016-04-02 2023-10-01 Res Inst Nationwide Childrens Hospital The U6 precursor system is optimized for tissue-specific expression
AU2017249283A1 (en) 2016-04-11 2018-10-25 Bayer Cropscience Nv Seed-specific and endosperm-preferential promoters and uses thereof
AU2017249663B2 (en) 2016-04-11 2023-04-20 BASF Agricultural Solutions Seed US LLC Seed-specific and endosperm-preferential promoters and uses thereof
WO2017178368A1 (en) 2016-04-13 2017-10-19 Bayer Cropscience Nv Seed-specific and embryo-preferential promoters and uses thereof
US10900046B2 (en) 2016-04-13 2021-01-26 BASF Agricultural Solutions Seed US LLC Seed- and funiculus-preferential promoters and uses thereof
WO2018089099A1 (en) 2016-10-11 2018-05-17 Dow Agrosciences Llc Modulation of transgene expression in plants
EP3573448A4 (en) 2017-01-28 2020-09-02 Inari Agriculture, Inc. NEW PLANT CELLS, PLANTS AND SEEDS
US11542519B2 (en) 2017-03-23 2023-01-03 Basf Se Anther-specific promoter and uses thereof
BR112019024630A2 (pt) 2017-05-24 2020-06-23 BASF Agricultural Solutions Seed US LLC Ácido nucleico isolado, gene recombinante, célula hospedeira, planta, partes e sementes de plantas, planta ou célula vegetal ou parte de planta ou semente, métodos para produzir uma planta transgênica e para efetuar a expressão induzível e uso do ácido nucleico
US11603536B2 (en) 2017-09-29 2023-03-14 Inari Agriculture Technology, Inc. Methods for efficient maize genome editing
CA3086620A1 (en) 2018-01-12 2019-07-18 Basf Se Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a
US11802288B1 (en) 2018-01-29 2023-10-31 Inari Agriculture Technology, Inc. Methods for efficient soybean genome editing
US11220694B1 (en) 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
EP3814490A2 (en) 2018-06-27 2021-05-05 Basf Se Thermostable rubisco activase and uses thereof
WO2020028850A1 (en) * 2018-08-03 2020-02-06 The Regents Of The University Of California Srnas that enhance plant resistance
WO2021004838A2 (en) 2019-07-05 2021-01-14 BASF Agricultural Solutions Seed US LLC Rubisco activase with reduced adp inhibition and uses thereof
CN110423750A (zh) * 2019-07-17 2019-11-08 中国农业科学院棉花研究所 棉花纤维长度相关microRNA396及其前体DNA和应用
WO2021092251A1 (en) 2019-11-05 2021-05-14 Apeel Technology, Inc. Prediction of infection in plant products
CN111304198B (zh) * 2019-12-13 2020-10-09 南京林业大学 春兰miR390b在控制植物营养器官发育中的应用
CN113005122B (zh) * 2019-12-20 2022-12-02 中国农业大学 一种抗玉米病毒的小rna
CA3165169A1 (en) 2019-12-20 2021-06-24 Pairwise Plants Services, Inc. Mutation of growth regulating factor family transcription factors for enhanced plant growth
WO2022020378A1 (en) 2020-07-20 2022-01-27 Flagship Pioneering, Inc. Viroid-derived polynucleotides for modifications of plants
KR20220027341A (ko) 2020-08-26 2022-03-08 (주)아모레퍼시픽 식물 유래 올리고뉴클레오티드를 포함하는 피부 장벽기능 강화용 조성물
KR20220027342A (ko) 2020-08-26 2022-03-08 (주)아모레퍼시픽 식물 유래 올리고뉴클레오티드를 포함하는 멜라닌 생성 조절용 조성물
TW202305130A (zh) 2021-03-26 2023-02-01 美商旗艦先鋒創新有限責任（Ｖｉｉ）公司 原核系統中環狀多核糖核苷酸之產生
EP4314277A1 (en) 2021-03-26 2024-02-07 Flagship Pioneering Innovations VII, LLC Compositions and methods for producing circular polyribonucleotides
TW202300650A (zh) 2021-03-26 2023-01-01 美商旗艦先鋒創新有限責任（Ｖｉｉ）公司 真核系統中環狀多核糖核苷酸的產生
CN113444741B (zh) * 2021-06-30 2022-05-20 西南大学 下调表达Bna-miR168a在改良油菜性状中应用
WO2023052562A1 (en) 2021-10-01 2023-04-06 Basf Se Wheat plants with an increased yield
CA3232731A1 (en) 2021-10-01 2023-04-06 Marie-Therese Scheirlinck Plants with improved properties
KR20240110818A (ko) 2021-11-01 2024-07-16 플래그쉽 파이어니어링 이노베이션스 Vii, 엘엘씨 유기체를 변형하기 위한 폴리뉴클레오티드
US20250230452A1 (en) 2022-01-20 2025-07-17 Flagship Pioneering Innovations Vii, Llc Polynucleotides for modifying organisms
WO2024099765A2 (en) 2022-11-10 2024-05-16 BASF Agricultural Solutions Seed US LLC Transcription regulating nucleotide sequences and methods of use
AR132587A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de martellivirales artificiales
WO2024229359A2 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Artificial tymovirales satellite rnas
WO2024229403A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Endornaviral satellite rna amplification systems for plants
WO2024229385A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Artificial ghabrivirales satellite rnas
AR132591A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de secoviridae artificiales
WO2024229395A1 (en) 2023-05-03 2024-11-07 Flagship Pioneering Innovations Vii, Llc Partitiviral satellite rna amplification systems for plants
AR133211A1 (es) 2023-05-03 2025-09-10 Flagship Pioneering Innovations Vii Llc Arn satélite artificiales de tombusviridae
AR132586A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de amalgavirus artificiales
AR132589A1 (es) 2023-05-03 2025-07-16 Flagship Pioneering Innovations Vii Llc Arn satélite de solemoviridae artificiales
EP4497829A1 (en) * 2023-07-28 2025-01-29 Consejo Superior De Investigaciones Cientificas (Csic) Minimal precursors of syn-tasirnas
CN117050998B (zh) * 2023-08-25 2024-02-09 青岛农业大学 一种miRNA在防治溴氰虫酰胺抗性烟粉虱中的应用

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034322A (en) 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5569587A (en) 1986-04-18 1996-10-29 Carnegie Mellon University Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes
US5627027A (en) 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5268486A (en) 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5728558A (en) 1988-02-03 1998-03-17 Pioneer Hi-Bred International, Inc. Molecular methods of hybrid seed production
US5250515A (en) 1988-04-11 1993-10-05 Monsanto Company Method for improving the efficacy of insect toxins
US5268526A (en) 1988-07-29 1993-12-07 E. I. Du Pont De Nemours And Company Overexpression of phytochrome in transgenic plants
US5589615A (en) 1988-10-14 1996-12-31 Plant Genetic Systems N.V. Process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins
DK0413019T3 (da) 1989-02-24 2001-11-12 Monsanto Technology Llc Syntetiske plantegener og fremgangsmåde til fremstilling af disse
JP3173784B2 (ja) 1990-06-25 2001-06-04 モンサント カンパニー グリホセート耐性植物
JPH08500003A (ja) 1992-02-19 1996-01-09 オレゴン州 翻訳不能なプラスセンスウイルスｒｎａの導入による耐ウイルス性植物の生産
US5773691A (en) * 1992-03-19 1998-06-30 E. I. Du Pont De Nemours And Company Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants
CA2100116C (en) 1992-07-09 2001-09-18 Joseph M. Jilka Virus resistant plants
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
ATE276368T1 (de) 1994-10-26 2004-10-15 Cargill Inc Fae1gene und deren anwendungen
US5773692A (en) 1995-12-12 1998-06-30 Her Majesty The Queen In Right Of Canada, As Represented By Agriculture And Agri-Food Canada Anti-sense RNA for CAB transcript to reduce chlorophyll content in plants
AUPN903196A0 (en) 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
US5800996A (en) 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US6376754B1 (en) 1997-03-07 2002-04-23 Asgrow Seed Company Plants having resistance to multiple herbicides and its use
US6107549A (en) 1998-03-10 2000-08-22 Monsanto Company Genetically engineered plant resistance to thiazopyr and other pyridine herbicides
US5914451A (en) 1998-04-06 1999-06-22 Monsanto Company Efficiency soybean transformation protocol
US6506599B1 (en) 1999-10-15 2003-01-14 Tai-Wook Yoon Method for culturing langerhans islets and islet autotransplantation islet regeneration
CN1333835A (zh) 1998-11-17 2002-01-30 孟山都技术有限公司 膦酸代谢植物
JP2002534129A (ja) 1999-01-14 2002-10-15 モンサント テクノロジー エルエルシー ダイズ形質転換方法
GB9925459D0 (en) 1999-10-27 1999-12-29 Plant Bioscience Ltd Gene silencing
WO2001068836A2 (en) * 2000-03-16 2001-09-20 Genetica, Inc. Methods and compositions for rna interference
US7109033B2 (en) 2000-08-24 2006-09-19 The Scripps Research Institute Stress-regulated genes of plants, transgenic plants containing same, and methods of use
AUPR210300A0 (en) 2000-12-15 2001-01-25 Grain Biotechnology Australia Pty Ltd Infection resistant plants and methods for their generation
CA2526831C (en) 2001-05-18 2012-07-31 Sirna Therapeutics, Inc. Rna interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (sina)
US20030135888A1 (en) * 2001-09-26 2003-07-17 Tong Zhu Genes that are modulated by posttranscriptional gene silencing
US20030150017A1 (en) 2001-11-07 2003-08-07 Mesa Jose Ramon Botella Method for facilitating pathogen resistance
BR0307253A (pt) * 2002-01-30 2004-12-14 Arborgen Llc Construtos de dna, sistema de expressão, métodos de supressão da expressão de um gene alvo numa célula, célula de planta transformada, planta transgênica e kit de supressão de expressão genética
US20040053876A1 (en) * 2002-03-26 2004-03-18 The Regents Of The University Of Michigan siRNAs and uses therof
ES2465574T3 (es) * 2002-05-03 2014-06-06 Duke University Un método para regular la expresión génica
BR0312580A (pt) 2002-07-10 2006-10-10 Univ Kansas State composições e métodos para controlar nematódeos parasitas
WO2004009779A2 (en) 2002-07-19 2004-01-29 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
AU2003298095A1 (en) * 2002-10-18 2004-05-04 Cropdesign N.V. Identification of e2f target genes and uses thereof
US20040261149A1 (en) 2003-02-24 2004-12-23 Fauquet Claude M. siRNA-mediated inhibition of gene expression in plant cells
US20060200878A1 (en) 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
EP1838144B1 (en) * 2005-01-07 2016-08-31 Oregon State University Method to trigger rna interference
US20070066521A1 (en) 2005-04-13 2007-03-22 Fauquet Claude M Short RNA-binding proteins
CA2612252A1 (en) 2005-06-17 2006-12-28 Pioneer Hi-Bred International, Inc. Methods and compositions for gene silencing
WO2007014370A2 (en) 2005-07-28 2007-02-01 University Of Delaware Small regulatory rnas and methods of use
CN101268194A (zh) 2005-09-20 2008-09-17 巴斯福植物科学有限公司 使用ta-siRNA调控基因表达的方法
CA2527447C (en) 2005-11-18 2015-05-05 Allen Vi Cuong Chan Message oriented construction of web services

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