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AR049215A1 - Homologos de galactinol sintetasa en plantas - Google Patents

Homologos de galactinol sintetasa en plantas

Info

Publication number
AR049215A1
AR049215A1 ARP050102361A ARP050102361A AR049215A1 AR 049215 A1 AR049215 A1 AR 049215A1 AR P050102361 A ARP050102361 A AR P050102361A AR P050102361 A ARP050102361 A AR P050102361A AR 049215 A1 AR049215 A1 AR 049215A1
Authority
AR
Argentina
Prior art keywords
galactinol
synthetase
nucleotide sequence
complement
polypeptide
Prior art date
Application number
ARP050102361A
Other languages
English (en)
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of AR049215A1 publication Critical patent/AR049215A1/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823Reproductive tissue-specific promoters
    • C12N15/8234Seed-specific, e.g. embryo, endosperm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/30Fuel from waste, e.g. synthetic alcohol or diesel

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente trata de homologos de galactinol sintetasa en palntas, también construcciones recombinantes para usar en la alteracion de la expresion de genes endogenos que codifican galactinol sintetasa y modular el nivel de sacáridos de rafinosa en células vegetales. Reivindicacion 1: Un polinucleotido aislado caracterizado porque comprende: (a) una secuencia de nucleotidos que codifica un polipéptido que tiene actividad de galactinol sintetasa, donde el polipéptido tiene una secuencia de aminoácidos con al menos 85% de identidad, cuando se la compara con una de SEQ ID No. 2 o 4, o 95% de identidad cuando se la compara con una de SEQ ID No. 6, sobre la base del método de alineamiento Clustal V, (b) la totalidad o una parte del polinucleotido aislado que comprende (a), para usar en la co-supresion o la supresion antisentido de secuencias de ácidos nucleicos endogenas que codifican polipéptidos que tienen actividad de galactinol sintetasa, o (c) un complemento de la secuencia de nucleotidos de (a) o (b), donde el complemento y la secuencia de nucleotidos consisten en la misma cantidad de nucleotidos y son 100% complementarios.
ARP050102361A 2004-06-22 2005-06-09 Homologos de galactinol sintetasa en plantas AR049215A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US58185104P 2004-06-22 2004-06-22

Publications (1)

Publication Number Publication Date
AR049215A1 true AR049215A1 (es) 2006-07-05

Family

ID=34972614

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050102361A AR049215A1 (es) 2004-06-22 2005-06-09 Homologos de galactinol sintetasa en plantas

Country Status (6)

Country Link
US (5) US7294756B2 (es)
EP (1) EP1759003A2 (es)
AR (1) AR049215A1 (es)
AU (2) AU2005264921B2 (es)
CA (2) CA2565306C (es)
WO (1) WO2006009659A2 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001251196A1 (en) * 2000-04-11 2001-10-23 E.I. Du Pont De Nemours And Company Plant raffinose saccharide biosynthetic enzymes
WO2005121347A2 (en) * 2004-06-09 2005-12-22 E.I. Dupont De Nemours And Company Recombinant constructs for use in reducing gene expression
EP2444497B1 (en) * 2004-09-24 2015-01-28 J.R. Simplot Company Gene silencing
BRPI0908694A2 (pt) 2008-05-23 2019-09-24 Du Pont ''sementes de grão de soja transgência e método para aumentar o teor de ácido graxo total de uma semente de grão de soja''
KR100993147B1 (ko) 2008-07-22 2010-11-09 한국생명공학연구원 작물 종자 특이적 유전자 증폭용 프라이머 세트, 방법 및 키트
PL2272327T3 (pl) 2009-04-16 2025-01-20 Benson Hill Seeds, Inc. Nasiona soi o wysokiej zdolności kiełkowania i ultraniskiej zawartości rafinozy i stachiozy
US9062317B2 (en) * 2011-05-09 2015-06-23 E I Du Pont De Nemours And Company Methods and compositions for silencing gene families using artificial microRNAs
ES2635115T3 (es) 2012-01-23 2017-10-02 E. I. Du Pont De Nemours And Company Regulación por disminución de expresión génica utilizando micro ARN artificiales para silenciar genes biosintéticos de ácidos grasos
WO2013151701A1 (en) * 2012-04-05 2013-10-10 Arborgen Inc. Improvement of freeze and drought tolerance in plants
US20160186195A1 (en) 2013-07-31 2016-06-30 E. I. Du Pont De Nemours And Company Modification of soybean seed composition to enhance feed, food and other industrial applications of soybean products
AR100159A1 (es) 2014-04-22 2016-09-14 Du Pont Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat
US11307139B2 (en) 2017-03-03 2022-04-19 Pioneer Hi-Bred International, Inc. Non-destructive assay for soybean seeds using near infrared analysis

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5648210A (en) 1991-07-24 1997-07-15 E. I. Du Pont De Nemours And Company Nucleotide sequences of galactinol synthase from zucchini and soybean
US6653451B1 (en) * 1991-10-24 2003-11-25 E. I. Du Pont De Nemours And Company Soybean products with improved carbohydrate composition and soybean plants
BR9815519A (pt) 1997-05-07 2001-11-06 Du Pont Fragmento de ácido nucleico isolado, gene quimérico, célula hospedeira transformada, método de alteração do nìvel de expressão de uma glicogenina vegetal, método de obtenção de um fragmento de ácido nucleico isolado e produto
US6100450A (en) * 1997-10-22 2000-08-08 Rhone-Poulenc Agrochimie Seed specific promoters based on arabidopsis genes
US20040031072A1 (en) * 1999-05-06 2004-02-12 La Rosa Thomas J. Soy nucleic acid molecules and other molecules associated with transcription plants and uses thereof for plant improvement
AU2001251196A1 (en) * 2000-04-11 2001-10-23 E.I. Du Pont De Nemours And Company Plant raffinose saccharide biosynthetic enzymes
JP2004512020A (ja) 2000-06-23 2004-04-22 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 組換え構築物ならびにこれを遺伝子発現を減少させる際に用いる方法
CA2483550C (en) 2002-05-09 2016-06-28 Cornell Research Foundation, Inc. Fagopyrum esculentum fagopyritol synthase gene and use thereof
WO2005121347A2 (en) * 2004-06-09 2005-12-22 E.I. Dupont De Nemours And Company Recombinant constructs for use in reducing gene expression

Also Published As

Publication number Publication date
US20080172758A1 (en) 2008-07-17
CA2856001A1 (en) 2006-01-26
US20090300799A1 (en) 2009-12-03
WO2006009659A3 (en) 2006-04-06
US7294756B2 (en) 2007-11-13
AU2011201873A1 (en) 2011-05-19
EP1759003A2 (en) 2007-03-07
US20100077510A1 (en) 2010-03-25
US20090100549A1 (en) 2009-04-16
CA2565306A1 (en) 2006-01-26
WO2006009659A2 (en) 2006-01-26
US20060005280A1 (en) 2006-01-05
CA2565306C (en) 2014-09-30
AU2005264921B2 (en) 2011-02-17
US7476778B2 (en) 2009-01-13
AU2005264921A1 (en) 2006-01-26

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