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AR047167A1 - Activacion de toxinas contra insectos en las plantas - Google Patents

Activacion de toxinas contra insectos en las plantas

Info

Publication number
AR047167A1
AR047167A1 ARP040104880A ARP040104880A AR047167A1 AR 047167 A1 AR047167 A1 AR 047167A1 AR P040104880 A ARP040104880 A AR P040104880A AR P040104880 A ARP040104880 A AR P040104880A AR 047167 A1 AR047167 A1 AR 047167A1
Authority
AR
Argentina
Prior art keywords
insects
modified
protoxin
plants
protease
Prior art date
Application number
ARP040104880A
Other languages
English (en)
Original Assignee
Du Pont
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Pioneer Hi Bred Int filed Critical Du Pont
Publication of AR047167A1 publication Critical patent/AR047167A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/641—Cysteine endopeptidases (3.4.22)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Catching Or Destruction (AREA)

Abstract

Se describen composiciones y métodos para proteger a las plantas contra plagas de insectos. En particular, se proveen secuencias de ácidos nucleicos que codifican protoxinas contra insectos modificadas en forma tal que comprendan al menos un sitio de activacion proteolítica sensible a una proteasa de la planta o una proteasa del intestino de insectos. La separacion de la protoxina modificada en el sitio de activacion proteolítica por una proteasa produce una toxina activa contra insectos. Se proveen métodos para usar las secuencias de ácidos nucleicos de la protoxina contra insectos modificada. En realizaciones específicas se proveen además composiciones y formulaciones que contienen la protoxina contra insectos modificada, y cassettes de expresion de la misma, así como plantas, y semillas. También se proveen ácidos nucleicos que codifican proteasas intestinales de insectos así como dichos polipéptidos.
ARP040104880A 2003-12-23 2004-12-22 Activacion de toxinas contra insectos en las plantas AR047167A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US53218503P 2003-12-23 2003-12-23

Publications (1)

Publication Number Publication Date
AR047167A1 true AR047167A1 (es) 2006-01-11

Family

ID=34738761

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP040104880A AR047167A1 (es) 2003-12-23 2004-12-22 Activacion de toxinas contra insectos en las plantas

Country Status (6)

Country Link
US (5) US7339092B2 (es)
EP (1) EP1709068A2 (es)
AR (1) AR047167A1 (es)
BR (1) BRPI0418365A (es)
CA (2) CA2551011C (es)
WO (1) WO2005063996A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7605304B2 (en) * 2000-10-24 2009-10-20 E.I. Du Pont De Nemours And Company Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
BRPI0611681B1 (pt) 2005-06-08 2018-01-16 E.I. Du Pont De Nemours And Company Moléculas de ácido nucléico isolada, cassete de expressão, métodos para proteger uma planta de uma praga de inseto, método de obtenção de uma planta transformada, protoxina isolada, composição, método para impactar uma praga de inseto
CN101113424B (zh) * 2007-07-04 2010-12-29 中国农业科学院植物保护研究所 对鞘翅目害虫高效的苏云金芽孢杆菌cry8G基因、蛋白及其应用
CN101130762B (zh) * 2007-08-07 2010-08-25 中国农业科学院植物保护研究所 对鞘翅目害虫高效的苏云金芽孢杆菌cry8H基因、蛋白及其应用
WO2011009182A2 (pt) 2009-07-24 2011-01-27 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Molécula de ácido nucléico isolada, construção gênica, vetor, célula transgênica, método para obtenção de uma célula e de uma planta transgênica, polipeptídeo isolado e purificado, composição pesticida biodegradável, método para o controle de uma praga, método de obtenção de linhagens transgênicas resistentes a um inseto praga
WO2011084314A2 (en) * 2009-12-21 2011-07-14 Pioneer Hi-Bred International, Inc. Novel bacillus thuringiensis gene with coleopteran activity
US20140205587A1 (en) * 2011-06-17 2014-07-24 Alvine Pharmaceuticals, Inc. Proteases for Degrading Gluten
WO2017192560A1 (en) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US11820791B2 (en) 2018-03-14 2023-11-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
AU2019234566B2 (en) 2018-03-14 2024-09-26 Hexima Limited Insecticidal proteins from plants and methods for their use
EP4182466A2 (en) 2020-07-14 2023-05-24 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2025178772A1 (en) 2024-02-23 2025-08-28 Genective Sa Insecticidal proteins compositions and methods of use
WO2025193453A1 (en) 2024-03-14 2025-09-18 Genective Sa Insecticidal proteins compositions and methods of use
WO2025235220A1 (en) 2024-05-08 2025-11-13 Genective Sa Insecticidal proteins compositions and methods of use
WO2025264577A1 (en) 2024-06-20 2025-12-26 Genective Sa Insecticidal proteins, compositions and methods of use
WO2025264584A1 (en) 2024-06-20 2025-12-26 Genective Sa Insecticidal proteins, compositions and methods of use
WO2026006045A1 (en) 2024-06-25 2026-01-02 Genective Sa Insecticidal proteins, compositions and methods of use

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US606059A (en) * 1898-06-21 Swinging straw-stacker
ES2071671T3 (es) * 1988-09-06 1995-07-01 Plant Genetic Systems Nv Plantas transformadas con una secuencia de adn originaria de bacillus thuringiensis, letales para los lepidopteros.
WO1998021348A1 (en) 1996-11-12 1998-05-22 Battelle Memorial Institute Method of producing human growth factors from whole plants or plant cell cultures
US6060594A (en) * 1997-12-18 2000-05-09 Ecogen, Inc. Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins
US7605304B2 (en) * 2000-10-24 2009-10-20 E.I. Du Pont De Nemours And Company Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans
FR2822157B1 (fr) 2001-03-19 2003-10-31 Aventis Cropscience Sa Toxine insecticide de bacillus thuringiensis modifiee sensible a la pepsine
US7230167B2 (en) * 2001-08-31 2007-06-12 Syngenta Participations Ag Modified Cry3A toxins and nucleic acid sequences coding therefor
BR0312064A (pt) * 2002-06-26 2005-08-09 Du Pont Genes que codificam proteìnas com atividade pesticida
BRPI0611681B1 (pt) * 2005-06-08 2018-01-16 E.I. Du Pont De Nemours And Company Moléculas de ácido nucléico isolada, cassete de expressão, métodos para proteger uma planta de uma praga de inseto, método de obtenção de uma planta transformada, protoxina isolada, composição, método para impactar uma praga de inseto

Also Published As

Publication number Publication date
CA2551011C (en) 2013-04-16
US7910807B2 (en) 2011-03-22
US20080200415A1 (en) 2008-08-21
CA2804953A1 (en) 2005-07-14
CA2551011A1 (en) 2005-07-14
US20080124799A1 (en) 2008-05-29
WO2005063996A3 (en) 2005-11-03
US8071736B2 (en) 2011-12-06
US7339092B2 (en) 2008-03-04
CA2804953C (en) 2016-02-23
US20080108127A1 (en) 2008-05-08
BRPI0418365A (pt) 2007-05-15
EP1709068A2 (en) 2006-10-11
WO2005063996A2 (en) 2005-07-14
US7629449B2 (en) 2009-12-08
US20090317891A1 (en) 2009-12-24
US7556936B2 (en) 2009-07-07
US20050166284A1 (en) 2005-07-28

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