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AR039113A1 - Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas - Google Patents

Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas

Info

Publication number
AR039113A1
AR039113A1 ARP030101010A ARP030101010A AR039113A1 AR 039113 A1 AR039113 A1 AR 039113A1 AR P030101010 A ARP030101010 A AR P030101010A AR P030101010 A ARP030101010 A AR P030101010A AR 039113 A1 AR039113 A1 AR 039113A1
Authority
AR
Argentina
Prior art keywords
gene
weight
dna sequences
ketoacyl
expressed
Prior art date
Application number
ARP030101010A
Other languages
English (en)
Inventor
Joanne J Fillatti
Neal A Bringe
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of AR039113A1 publication Critical patent/AR039113A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings or cooking oils
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • C—CHEMISTRY; METALLURGY
    • C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
    • C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
    • C10L1/00—Liquid carbonaceous fuels
    • C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
    • C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B1/00—Production of fats or fatty oils from raw materials
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Animal Husbandry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Reivindicación 1: Una semilla de soja, caracterizada porque presenta una composición de aceites que comprende entre 55 y 75% en peso de ácido oleico, entre 10 y 30% en peso de ácido linoleico, 4% o menos en peso de ácido linolénico y 6% o menos en peso de ácidos grasos saturados. Reivindicación 2: La semilla de soja de la cláusula 1, caracterizada porque dicha semilla comprende una molécula de ácido nucleico recombinante, donde dicha molécula comprende un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de al menos dos genes seleccionados del grupo formado por los genes FAD2, FAD3 y FATB y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa. Reivindicación 8: Aceite derivado de la semilla de soja de la cláusula 2, caracterizado porque dicho aceite presenta un contenido reducido de ácidos grasos saturados, un contenido reducido de ácidos grasos poliinsaturados y un mayor contenido de ácido oleico con relación a un aceite derivado de las semillas de una planta de antecedentes genéticos similares pero que no contiene la molécula de ácido nucleico recombinante. Reivindicación 11: Una semilla de soja caracterizada porque presenta una composición de aceites que comprende entre 69 y 73% en peso de ácido oleico, entre 21 y 24% en peso de ácido linoleico, entre 0,5 y 3% en peso de ácido linolénico y 2-3% en peso de ácidos grasos saturados. Reivindicación 12: Un aceite de soja crudo, caracterizado porque presenta una composición de aceites que comprende entre 55 y 75% en peso de ácido oleico, entre 10 y 30% en peso de ácido linoleico, 4% o menos en peso de ácido linolénico y 6% o menos en peso de ácidos grasos saturados. Reivindicación 17: Una planta de soja transformada portadora de semillas, caracterizada porque dichas semillas presentan una composición de aceites que comprende entre 55 y 75% en peso de ácido oleico, entre 10 y 30% en peso de ácido linoleico, 4% o menos en peso de ácido linolénico y 6% o menos en peso de ácidos grasos saturados. Reivindicación 18: La planta de soja transformada de la cláusula 17, caracterizada porque dicha planta de soja transformada comprende una molécula de ácido nucleico recombinante que comprende: un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de un gen de FAD2 y un gen de FAD3 y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa. Reivindicación 27: Un método para alterar la composición de aceites de una célula vegetal, caracterizado porque comprende: (A) transformar una célula vegetal con una molécula de ácido nucleico recombinante que comprende un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de al menos dos genes seleccionados del grupo formado por los genes FAD2, FAD3 y FATB y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa; y (B) cultivar dicha célula vegetal bajo condiciones que permitan la transcripción de dicho primer conjunto de secuencias de ADN y dicho segundo conjunto de secuencias de ADN, con lo cual se altera la composición de aceites con relación a una célula vegetal de antecedentes genéticos similares pero que no contiene la molécula de ácido nucleico recombinante. Reivindicación 36: Una molécula de ácido nucleico recombinante, caracterizada porque comprende: un primer conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de suprimir la expresión endógena de al menos dos genes seleccionados del grupo formado por los genes FAD2, FAD3 y FATB; y un segundo conjunto de secuencias de ADN que, cuando se expresa en una célula huésped, tiene la capacidad de incrementar la expresión endógena de al menos un gen seleccionado del grupo formado por un gen de b-cetoacil-ACP sintetasa I, un gen de b-cetoacil-ACP sintetasa IV y un gen de d-9-desaturasa.
ARP030101010A 2002-03-21 2003-03-21 Construcciones de acidos nucleicos y metodos para producir composiciones de aceites de semillas alteradas AR039113A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36579402P 2002-03-21 2002-03-21
US39018502P 2002-06-21 2002-06-21

Publications (1)

Publication Number Publication Date
AR039113A1 true AR039113A1 (es) 2005-02-09

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Country Link
US (3) US20060080750A1 (es)
EP (2) EP1484959A4 (es)
CN (1) CN100510084C (es)
AR (1) AR039113A1 (es)
AU (1) AU2003214247B2 (es)
BR (1) BR0308614A (es)
CA (1) CA2479587A1 (es)
MX (1) MXPA04009134A (es)
WO (1) WO2003080802A2 (es)
ZA (1) ZA200407546B (es)

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