AR010218A1 - A FAMILY OF MULTI-UNIT BACTERIAL PROTEINS - Google Patents
A FAMILY OF MULTI-UNIT BACTERIAL PROTEINSInfo
- Publication number
- AR010218A1 AR010218A1 ARP970104115A ARP970104115A AR010218A1 AR 010218 A1 AR010218 A1 AR 010218A1 AR P970104115 A ARP970104115 A AR P970104115A AR P970104115 A ARP970104115 A AR P970104115A AR 010218 A1 AR010218 A1 AR 010218A1
- Authority
- AR
- Argentina
- Prior art keywords
- shiga
- toxins
- purification
- describes
- toxoids
- Prior art date
Links
- 108010077805 Bacterial Proteins Proteins 0.000 title 1
- 108010017898 Shiga Toxins Proteins 0.000 abstract 3
- 238000000746 purification Methods 0.000 abstract 3
- 108010079723 Shiga Toxin Proteins 0.000 abstract 2
- 108020001507 fusion proteins Proteins 0.000 abstract 2
- 102000037865 fusion proteins Human genes 0.000 abstract 2
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 231100000331 toxic Toxicity 0.000 abstract 1
- 230000002588 toxic effect Effects 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
- 108700012359 toxins Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/25—Shigella (G)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Describe la aislacion y purificacion de toxinas Shiga marcadas con histidina (marcadas con His) biologica e inmunologicamente activas, toxinasasociadas con HC y la secuela HUS potencialmente peligrosa para la salud transmitida por cepas de bacterias patogenas. Describe como el marcado con Hissimplifica mucho y acelera la purificacion de las toxinas Shiga y describe un método mejorado para tal purificacion. Un aspecto es obtener y usar toxoidesShiga que son inmunorreactivos perono toxicos. Otro aspecto es obtener y usar proteínas de fusion de toxinas o toxoides Shiga marcados con His. Otro aspectoes obtener y usar anticuerpos a toxinas Shiga marcadas con His, toxoides o proteínas de fusion de toxinas/toxoides Shiga.Describes the isolation and purification of biologically and immunologically active histidine-labeled (His-labeled) Shiga toxins, HC-associated toxins and the potentially dangerous health-related HUS sequel transmitted by strains of pathogenic bacteria. It describes how Hiss tagging greatly simplifies and accelerates the purification of Shiga toxins and describes an improved method for such purification. One aspect is to obtain and use Shiga toxoids that are immunoreactive but not toxic. Another aspect is to obtain and use His tagged Shiga toxin or toxoid fusion proteins. Another aspect is obtaining and using antibodies to His tagged Shiga toxins, toxoids, or Shiga toxin / toxoid fusion proteins.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2563796P | 1996-09-10 | 1996-09-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR010218A1 true AR010218A1 (en) | 2000-06-07 |
Family
ID=21827219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP970104115A AR010218A1 (en) | 1996-09-10 | 1997-09-09 | A FAMILY OF MULTI-UNIT BACTERIAL PROTEINS |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0929679A2 (en) |
| JP (1) | JP2001500730A (en) |
| AR (1) | AR010218A1 (en) |
| AU (1) | AU4184597A (en) |
| CA (1) | CA2265887A1 (en) |
| WO (1) | WO1998011229A2 (en) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2766193B1 (en) | 1997-07-18 | 2001-09-14 | Inst Curie | CHEMICAL POLYPEPTIDE COMPRISING FRAGMENT B OF TOXIN SHIGA AND PEPTIDES OF THERAPEUTIC INTEREST |
| GB9715177D0 (en) * | 1997-07-21 | 1997-09-24 | Neutec Pharma Plc | Medicament |
| CA2333921C (en) * | 1998-05-15 | 2014-04-22 | Allan M. Green | Verotoxin b subunit for immunization |
| US7157418B1 (en) | 1998-07-22 | 2007-01-02 | Osprey Pharmaceuticals, Ltd. | Methods and compositions for treating secondary tissue damage and other inflammatory conditions and disorders |
| EP1057895A1 (en) * | 1999-06-04 | 2000-12-06 | Lohmann Animal Health GmbH & Co. KG | Fusion protein comprising Shiga toxin 2e B subunit, (vaccine)compositions comprising it, and methods for their production |
| US8846058B2 (en) | 2006-02-16 | 2014-09-30 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine, Inc. | Shiga toxoid chimeric proteins |
| WO2007124133A2 (en) * | 2006-04-20 | 2007-11-01 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine Incorporated | Methods and compositions based on shiga toxin type 1 protein |
| PE20120425A1 (en) | 2009-01-23 | 2012-05-03 | Jackson H M Found Military Med | METHODS AND COMPOSITIONS BASED ON TYPE 2 PROTEIN OF SHIGA TOXIN |
| MX378807B (en) * | 2013-03-12 | 2025-03-11 | Molecular Templates Inc | CD20-BINDING IMMUNOTOXINS FOR INDUCING CELLULAR INTERNALIZATION AND METHODS USING THEM. |
| IL302552B2 (en) | 2014-01-27 | 2024-09-01 | Molecular Templates Inc | Polypeptides that remove an epitope for MHC group I |
| JP6935195B2 (en) * | 2014-03-11 | 2021-09-15 | モレキュラー テンプレーツ, インク.Molecular Templates, Inc. | Protein containing binding region, effector region of Shiga toxin A subunit, and carboxy-terminal endoplasmic reticulum localization signal motif |
| US11142584B2 (en) | 2014-03-11 | 2021-10-12 | Molecular Templates, Inc. | CD20-binding proteins comprising Shiga toxin A subunit effector regions for inducing cellular internalization and methods using same |
| SMT202100379T1 (en) * | 2014-03-11 | 2021-09-14 | Molecular Templates Inc | Proteins comprising amino-terminal proximal shiga toxin a subunit effector regions and cell-targeting immunoglobulin-type binding regions capable of specifically binding cd38 |
| CA2947048C (en) | 2014-06-11 | 2023-10-17 | Molecular Templates, Inc. | Protease-cleavage resistant, shiga toxin a subunit effector polypeptides and cell-targeted molecules comprising the same |
| EP3253799B1 (en) | 2015-02-05 | 2020-12-02 | Molecular Templates, Inc. | Multivalent cd20-binding molecules comprising shiga toxin a subunit effector regions and enriched compositions thereof |
| EP3636660A1 (en) | 2015-05-30 | 2020-04-15 | Molecular Templates, Inc. | De-immunized, shiga toxin a subunit scaffolds and cell-targeting molecules comprising the same |
| KR102580647B1 (en) | 2016-12-07 | 2023-09-20 | 몰레큘러 템플레이츠, 인코퍼레이션. | Shiga toxin A subunit effector polypeptides, Shiga toxin effector scaffolds, and cell-targeting molecules for site-specific conjugation |
| CA3049456A1 (en) | 2017-01-25 | 2018-08-02 | Molecular Templates, Inc. | Cell-targeting molecules comprising de-immunized, shiga toxin a subunit effectors and cd8+ t-cell epitopes |
| MX2019009726A (en) | 2018-04-17 | 2020-02-05 | Molecular Templates Inc | Her2-targeting molecules comprising de-immunized, shiga toxin a subunit scaffolds. |
| KR102869667B1 (en) * | 2023-01-13 | 2025-10-16 | 전북대학교산학협력단 | Mass Production Method of Recombinant Stx2e Toxoid and Swine Edema Disease Vaccine Composition |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI971732L (en) * | 1994-10-24 | 1997-06-23 | Ophidian Pharm Inc | Vaccine and antitoxin to treat and prevent disease caused by C. difficile |
| AU5431196A (en) * | 1995-03-24 | 1996-10-16 | Ophidian Pharmaceuticals | Treatment for verotoxin-producing escherichia coli |
-
1997
- 1997-09-09 EP EP97939845A patent/EP0929679A2/en not_active Withdrawn
- 1997-09-09 WO PCT/US1997/015836 patent/WO1998011229A2/en not_active Ceased
- 1997-09-09 AR ARP970104115A patent/AR010218A1/en unknown
- 1997-09-09 JP JP10513748A patent/JP2001500730A/en active Pending
- 1997-09-09 AU AU41845/97A patent/AU4184597A/en not_active Abandoned
- 1997-09-09 CA CA002265887A patent/CA2265887A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998011229A3 (en) | 1998-04-16 |
| CA2265887A1 (en) | 1998-03-19 |
| EP0929679A2 (en) | 1999-07-21 |
| AU4184597A (en) | 1998-04-02 |
| JP2001500730A (en) | 2001-01-23 |
| WO1998011229A2 (en) | 1998-03-19 |
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