AR003407A1 - Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasa - Google Patents
Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasaInfo
- Publication number
- AR003407A1 AR003407A1 ARP960101380A AR10138096A AR003407A1 AR 003407 A1 AR003407 A1 AR 003407A1 AR P960101380 A ARP960101380 A AR P960101380A AR 10138096 A AR10138096 A AR 10138096A AR 003407 A1 AR003407 A1 AR 003407A1
- Authority
- AR
- Argentina
- Prior art keywords
- delta
- sterol
- procedure
- reductase
- protein
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 9
- 102000004169 proteins and genes Human genes 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 238000000338 in vitro Methods 0.000 title abstract 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 2
- 229930182558 Sterol Natural products 0.000 title abstract 2
- 238000010367 cloning Methods 0.000 title abstract 2
- 230000007812 deficiency Effects 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 235000003702 sterols Nutrition 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 238000002405 diagnostic procedure Methods 0.000 title 1
- 150000003432 sterols Chemical class 0.000 title 1
- ORNBQBCIOKFOEO-YQUGOWONSA-N Pregnenolone Natural products O=C(C)[C@@H]1[C@@]2(C)[C@H]([C@H]3[C@@H]([C@]4(C)C(=CC3)C[C@@H](O)CC4)CC2)CC1 ORNBQBCIOKFOEO-YQUGOWONSA-N 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 238000001727 in vivo Methods 0.000 abstract 1
- ORNBQBCIOKFOEO-QGVNFLHTSA-N pregnenolone Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 ORNBQBCIOKFOEO-QGVNFLHTSA-N 0.000 abstract 1
- 229960000249 pregnenolone Drugs 0.000 abstract 1
- 125000002328 sterol group Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P33/00—Preparation of steroids
- C12P33/02—Dehydrogenating; Dehydroxylating
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Una secuencia de ADNc que codifica una proteína de A, thaliana que posee actividad de delta-5,7-esterol, delta-7-reductasa (secuencia SEC ID N°1);proteína delta-7RED; anticuerpo dirigido contra dicha proteína, vector de expresión, célulahos pedante, un procedimiento de clonación del ácido nucleico,procedimiento de preparación de la proteína, un procediento de reducción in vitro e in vivo de un esterol insaturado en la posición C-7, un procedimientode producción de pregnenolona,cepa de levadura transformada y método de adición de la deficiencia de delta-5,7-esterol, delta-7-reductasa.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9501723A FR2730494B1 (fr) | 1995-02-15 | 1995-02-15 | Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta7-red, procede de production, souches de levures transformees, applications |
| FR9506517A FR2734839B1 (fr) | 1995-06-01 | 1995-06-01 | Sequence d'adn codant pour une proteine d'a. thaliana ayant une activite delta-5,7 sterol, delta-7 reductase, proteine delta-7red, procede de production, souches de levures transformees, applications. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR003407A1 true AR003407A1 (es) | 1998-08-05 |
Family
ID=26231759
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP960101380A AR003407A1 (es) | 1995-02-15 | 1996-02-14 | Procedimiento de clonacion de un acido nucleico que codifica una proteina con actividad de delta-5,7-esterol, delta-7-reductasa, procedimiento de preparacion de la proteina, procemiento de reduccion in vitro de un esterol insaturado, procedimiento de produccion de pregnenolona, cepa de levadura transformada y metodo de diagnostico in vitro para la deteccion de la deficiencia de delta-5, 7-esterol, delta-7-reductasa |
| ARP060102688A AR053931A2 (es) | 1995-02-15 | 2006-06-22 | Secuencia de adn que codifica una proteina de a. thaliana que posee actividad de delta -5,7-esterol, delta-7-reductasa, proteina delta-7red |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP060102688A AR053931A2 (es) | 1995-02-15 | 2006-06-22 | Secuencia de adn que codifica una proteina de a. thaliana que posee actividad de delta -5,7-esterol, delta-7-reductasa, proteina delta-7red |
Country Status (24)
| Country | Link |
|---|---|
| US (3) | US5759801A (es) |
| EP (1) | EP0727489B1 (es) |
| JP (1) | JP4082469B2 (es) |
| KR (1) | KR100422293B1 (es) |
| CN (2) | CN1216995C (es) |
| AR (2) | AR003407A1 (es) |
| AT (1) | ATE457354T1 (es) |
| BR (1) | BRPI9600729B8 (es) |
| CA (1) | CA2169524C (es) |
| CZ (1) | CZ294860B6 (es) |
| DE (1) | DE69638123D1 (es) |
| DK (1) | DK0727489T3 (es) |
| ES (1) | ES2339833T3 (es) |
| FI (1) | FI120877B (es) |
| HR (1) | HRP960078B1 (es) |
| HU (1) | HU220587B1 (es) |
| IL (4) | IL116872A (es) |
| PL (1) | PL185569B1 (es) |
| PT (1) | PT727489E (es) |
| RU (1) | RU2242517C2 (es) |
| SI (1) | SI0727489T1 (es) |
| SK (1) | SK283656B6 (es) |
| TR (1) | TR199600116A2 (es) |
| UA (1) | UA72172C2 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0954568A1 (en) * | 1996-06-21 | 1999-11-10 | The General Hospital Corporation | Plant sterol reductases and uses thereof |
| DE19739940A1 (de) * | 1997-09-11 | 1999-03-18 | Hartmut Prof Dr Med Glossmann | Protein mit Aminosäuresequenz der DELTA7-Sterolreduktase, isoliertes cDNA-Molekül, das für das Protein kodiert, rekombinanter DNA-Vektor und biologisch aktive Zusammensetzungen, die das Protein oder das cDNA-Molekül enthalten |
| FR2774393B1 (fr) * | 1998-02-05 | 2000-03-24 | Hoechst Marion Roussel Inc | Souches de levure ayant le gene atf2 interrompu et leurs applications |
| US6465717B1 (en) * | 1998-11-20 | 2002-10-15 | E. I. Du Pont De Nemours And Company | Sterol metabolism enzymes |
| FR2812884B1 (fr) * | 2000-08-08 | 2002-11-08 | Aventis Pharma Sa | Levures modifiees et utilisations, notamment pour la production de derives steroidiens |
| FR2820145B1 (fr) * | 2001-01-31 | 2004-01-23 | Aventis Pharma Sa | Souche de levure produisant des steroides de facon autonome |
| US7193169B2 (en) * | 2003-10-29 | 2007-03-20 | Alcon, Inc. | Ergonomic footswitch |
| FR2869914B1 (fr) | 2004-05-06 | 2012-11-09 | Aventis Pharma Sa | Souches de levure produisant du cholesterol et leurs applications |
| ATE521710T1 (de) * | 2005-03-16 | 2011-09-15 | Metabolix Inc | Chemisch induzierbare expression von biosynthesewegen |
| KR100672006B1 (ko) * | 2005-08-03 | 2007-01-19 | 길병옥 | 폐자재를 이용한 형강의 제조방법 및 그 제조방법에 의해생산된 형강 |
| BRPI0821401A2 (pt) | 2007-12-21 | 2014-10-14 | Novartis Ag | Compostos orgânicos |
| DE102009022772A1 (de) * | 2009-05-21 | 2010-11-25 | Organobalance Gmbh | Mikroorganismus zur Expression eines humanen Membranproteins |
| FR2961218A1 (fr) | 2010-06-10 | 2011-12-16 | Sanofi Aventis | Methode de detection de composes modulateurs du metabolisme du cholesterol |
| CN102286053B (zh) * | 2011-06-20 | 2012-09-05 | 河北联合大学 | 孕甾烯醇酮吡咯化合物及其制备方法 |
| KR20160019451A (ko) * | 2013-06-17 | 2016-02-19 | 사노피 | 시토크롬 p450 모노옥시게나제 생체촉매작용을 위한 전세포 시스템 |
| CN110903993A (zh) * | 2019-12-20 | 2020-03-24 | 河北兰升生物科技有限公司 | 一种产生菜籽甾醇的酿酒酵母工程菌及其构建方法和应用 |
| CN112813129B (zh) * | 2021-02-05 | 2023-09-08 | 江南大学 | 利用区室化提高酵母菌中7-脱氢胆固醇产量的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2554986A (en) * | 1950-01-05 | 1951-05-29 | Upjohn Co | Synthesis of pregnenolone |
| JPS6447380A (en) * | 1987-08-19 | 1989-02-21 | Agency Ind Science Techn | Steroid-oxidizing yeast strain |
| CN1042567A (zh) * | 1988-09-23 | 1990-05-30 | 吉斯特-布罗卡迪斯公司 | 类固醇多步氧化方法和所用的遗传工程细胞 |
| JP2645130B2 (ja) * | 1989-03-31 | 1997-08-25 | 日清製粉株式会社 | ステロイド誘導体 |
| US5212167A (en) * | 1991-09-12 | 1993-05-18 | Trustees Of Boston University | Modulation of receptor-mediated ion transport |
| US5547868A (en) * | 1993-06-09 | 1996-08-20 | Regents Of The University Of California | Cholesterol disposal fusion enzymes |
-
1996
- 1996-01-23 IL IL11687296A patent/IL116872A/en not_active IP Right Cessation
- 1996-01-23 IL IL16247696A patent/IL162476A/en not_active IP Right Cessation
- 1996-02-12 US US08/601,435 patent/US5759801A/en not_active Expired - Lifetime
- 1996-02-13 UA UA96020528A patent/UA72172C2/uk unknown
- 1996-02-13 SK SK188-96A patent/SK283656B6/sk not_active IP Right Cessation
- 1996-02-14 CZ CZ1996432A patent/CZ294860B6/cs not_active IP Right Cessation
- 1996-02-14 BR BRPI9600729-0 patent/BRPI9600729B8/pt not_active IP Right Cessation
- 1996-02-14 PT PT96400301T patent/PT727489E/pt unknown
- 1996-02-14 CN CN961060778A patent/CN1216995C/zh not_active Expired - Lifetime
- 1996-02-14 AR ARP960101380A patent/AR003407A1/es active IP Right Grant
- 1996-02-14 CN CN2004100488331A patent/CN1664107A/zh active Pending
- 1996-02-14 DK DK96400301.6T patent/DK0727489T3/da active
- 1996-02-14 KR KR1019960003564A patent/KR100422293B1/ko not_active Expired - Lifetime
- 1996-02-14 RU RU96102857/13A patent/RU2242517C2/ru active
- 1996-02-14 AT AT96400301T patent/ATE457354T1/de active
- 1996-02-14 CA CA2169524A patent/CA2169524C/fr not_active Expired - Lifetime
- 1996-02-14 FI FI960663A patent/FI120877B/fi not_active IP Right Cessation
- 1996-02-14 SI SI9630775T patent/SI0727489T1/sl unknown
- 1996-02-14 ES ES96400301T patent/ES2339833T3/es not_active Expired - Lifetime
- 1996-02-14 DE DE69638123T patent/DE69638123D1/de not_active Expired - Lifetime
- 1996-02-14 HU HU9600325A patent/HU220587B1/hu unknown
- 1996-02-14 EP EP96400301A patent/EP0727489B1/fr not_active Expired - Lifetime
- 1996-02-15 JP JP05079896A patent/JP4082469B2/ja not_active Expired - Lifetime
- 1996-02-15 PL PL96312828A patent/PL185569B1/pl unknown
- 1996-02-15 TR TR96/00116A patent/TR199600116A2/xx unknown
- 1996-02-15 HR HR960078A patent/HRP960078B1/xx not_active IP Right Cessation
-
1997
- 1997-01-14 US US08/783,202 patent/US5989881A/en not_active Expired - Lifetime
- 1997-09-16 US US08/931,047 patent/US5965417A/en not_active Expired - Lifetime
-
2004
- 2004-06-10 IL IL16247704A patent/IL162477A0/xx not_active IP Right Cessation
- 2004-06-10 IL IL16247604A patent/IL162476A0/xx unknown
-
2006
- 2006-06-22 AR ARP060102688A patent/AR053931A2/es unknown
Also Published As
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Legal Events
| Date | Code | Title | Description |
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| FG | Grant, registration |