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AR002914A1 - A CHEMICALLY INDUCTIBLE GENE EXPRESSION CASSSETTE FOR VEGETABLES THAT INCLUDES A SEQUENCE THAT CODES AN INSECTICIDE PROTEIN, A VEGETABLE CELL, A PLANTS AND SEEDS TRANSFORMED WITH THE CASSETTE, A METHOD OF CONTROLLING INSECTS. - Google Patents

A CHEMICALLY INDUCTIBLE GENE EXPRESSION CASSSETTE FOR VEGETABLES THAT INCLUDES A SEQUENCE THAT CODES AN INSECTICIDE PROTEIN, A VEGETABLE CELL, A PLANTS AND SEEDS TRANSFORMED WITH THE CASSETTE, A METHOD OF CONTROLLING INSECTS.

Info

Publication number
AR002914A1
AR002914A1 ARP960103689A AR10368996A AR002914A1 AR 002914 A1 AR002914 A1 AR 002914A1 AR P960103689 A ARP960103689 A AR P960103689A AR 10368996 A AR10368996 A AR 10368996A AR 002914 A1 AR002914 A1 AR 002914A1
Authority
AR
Argentina
Prior art keywords
cassette
plant
gene
chemically
gene expression
Prior art date
Application number
ARP960103689A
Other languages
Spanish (es)
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Publication of AR002914A1 publication Critical patent/AR002914A1/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237Externally regulated expression systems
    • C12N15/8238Externally regulated expression systems chemically inducible, e.g. tetracycline
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un cassette de expresión génica para vegetales inducible químicamente que comprende un primer promotor ligado operativamente a una secuencia reguladoraderivada del gen alcR y que codifica una proteína reguladora, y un promotor inducible ligadooperativamente a un gen de interés que codifica una proteinadanina para insectos o cuya expresión induce una vía metabólica productora de un metabolito danino para insectos, con dicho promotor inducible activado porla proteina reguladora en presencia de un inductor exógeno efectivo por medio del cual la aplicación del inductor provoca la expresión del gen de interés. Lainvención también se refiere a una célula vegetal que contiene dicho cassette; un tejido vegetal y una planta que comprendela célula vegetal; una semilla quederiva de la planta y un método para controlar insectos que comprende la transformación de una célula vegetal.A chemically inducible plant gene expression cassette comprising a first promoter operably linked to a regulatory sequence derived from the alcR gene and encoding a regulatory protein, and an inducible promoter operatively linked to a gene of interest that encodes a protein proteinadanine for insects or whose expression induces a metabolic pathway producing a harmful metabolite for insects, with said inducible promoter activated by the regulatory protein in the presence of an effective exogenous inducer by means of which the application of the inducer causes the expression of the gene of interest. The invention also refers to a plant cell containing said cassette; a plant tissue and a plant comprising the plant cell; a seed derived from the plant and a method for controlling insects that involves the transformation of a plant cell.

ARP960103689A 1995-08-08 1996-07-22 A CHEMICALLY INDUCTIBLE GENE EXPRESSION CASSSETTE FOR VEGETABLES THAT INCLUDES A SEQUENCE THAT CODES AN INSECTICIDE PROTEIN, A VEGETABLE CELL, A PLANTS AND SEEDS TRANSFORMED WITH THE CASSETTE, A METHOD OF CONTROLLING INSECTS. AR002914A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9516241.8A GB9516241D0 (en) 1995-08-08 1995-08-08 Dna constructs

Publications (1)

Publication Number Publication Date
AR002914A1 true AR002914A1 (en) 1998-04-29

Family

ID=10778942

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP960103689A AR002914A1 (en) 1995-08-08 1996-07-22 A CHEMICALLY INDUCTIBLE GENE EXPRESSION CASSSETTE FOR VEGETABLES THAT INCLUDES A SEQUENCE THAT CODES AN INSECTICIDE PROTEIN, A VEGETABLE CELL, A PLANTS AND SEEDS TRANSFORMED WITH THE CASSETTE, A METHOD OF CONTROLLING INSECTS.

Country Status (19)

Country Link
EP (1) EP0846179A2 (en)
JP (1) JPH11510694A (en)
KR (1) KR19990036251A (en)
CN (1) CN1199425A (en)
AP (1) AP863A (en)
AR (1) AR002914A1 (en)
AU (1) AU704172B2 (en)
BR (1) BR9609873A (en)
CA (1) CA2227445A1 (en)
CZ (1) CZ36998A3 (en)
GB (1) GB9516241D0 (en)
HU (1) HUP9900057A3 (en)
IL (1) IL123172A0 (en)
MX (1) MX9801008A (en)
NZ (1) NZ313724A (en)
PL (1) PL324880A1 (en)
SK (1) SK16998A3 (en)
TR (1) TR199800177T1 (en)
WO (1) WO1997006268A2 (en)

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GB9902234D0 (en) * 1999-02-01 1999-03-24 Zeneca Ltd Expression system
GB9918154D0 (en) * 1999-08-02 1999-10-06 Zeneca Ltd Expression system
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WO2005014828A2 (en) 2003-08-01 2005-02-17 Basf Plant Science Gmbh Process for the production of fine chemicals in plants
WO2008015263A2 (en) 2006-08-02 2008-02-07 Cropdesign N.V. Plants having improved characteristics and a method for making the same
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WO2011074959A1 (en) 2009-12-15 2011-06-23 Edwin Henricus Antonius Holman Transgenic ozone-resistant plants
AR083029A1 (en) 2010-12-09 2013-01-23 Syngenta Participations Ag METHODS AND COMPOSITIONS THAT USE SMALL INTERFERENT ARN (ARNIP) FOR THE CONTROL OF NEMATODES IN PLANTS
CA2825388A1 (en) 2011-01-18 2012-07-26 Syngenta Participations Ag Methods and compositions for modified ethanol inducible promoter systems
EP2768950B1 (en) 2011-10-20 2018-03-07 University of Iowa Research Foundation N-demethyase genes and uses thereof
ES2670505T3 (en) 2012-02-16 2018-05-30 Syngenta Participations Ag Modified Pesticide Proteins
WO2013187957A1 (en) 2012-06-15 2013-12-19 University Of Iowa Research Foundation Caffeine responsive promoters and regulatory genes
MX370669B (en) 2012-06-22 2019-12-19 Syngenta Participations Ag Biological control of coleopteran pests.
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CA2948369C (en) 2014-04-17 2023-09-26 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
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Also Published As

Publication number Publication date
KR19990036251A (en) 1999-05-25
HUP9900057A2 (en) 1999-04-28
AP863A (en) 2000-08-04
AU6625296A (en) 1997-03-05
AP9801194A0 (en) 1998-03-31
GB9516241D0 (en) 1995-10-11
SK16998A3 (en) 1998-09-09
TR199800177T1 (en) 1998-05-21
AU704172B2 (en) 1999-04-15
WO1997006268A2 (en) 1997-02-20
JPH11510694A (en) 1999-09-21
WO1997006268A3 (en) 1997-03-13
EP0846179A2 (en) 1998-06-10
CN1199425A (en) 1998-11-18
HUP9900057A3 (en) 2001-06-28
NZ313724A (en) 1999-04-29
BR9609873A (en) 1999-03-23
CA2227445A1 (en) 1997-02-20
MX9801008A (en) 1998-04-30
CZ36998A3 (en) 1998-06-17
IL123172A0 (en) 1998-09-24
PL324880A1 (en) 1998-06-22

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