AR000061A1 - Medio para detectar dentro de un lapso de 24 horasla presencía o ausencia de enterococos en una mue stra liquida cuya procedencia excluye aquellas tomadas de seres humanos y animales superiores. - Google Patents
Medio para detectar dentro de un lapso de 24 horasla presencía o ausencia de enterococos en una mue stra liquida cuya procedencia excluye aquellas tomadas de seres humanos y animales superiores.Info
- Publication number
- AR000061A1 AR000061A1 AR33409095A AR33409095A AR000061A1 AR 000061 A1 AR000061 A1 AR 000061A1 AR 33409095 A AR33409095 A AR 33409095A AR 33409095 A AR33409095 A AR 33409095A AR 000061 A1 AR000061 A1 AR 000061A1
- Authority
- AR
- Argentina
- Prior art keywords
- enterococci
- medium
- sample
- hours
- humans
- Prior art date
Links
- 239000007788 liquid Substances 0.000 title abstract 4
- 241001465754 Metazoa Species 0.000 title abstract 3
- 230000007613 environmental effect Effects 0.000 abstract 2
- 238000011534 incubation Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 2
- 241000282412 Homo Species 0.000 abstract 1
- 241000282414 Homo sapiens Species 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
- 235000013343 vitamin Nutrition 0.000 abstract 1
- 239000011782 vitamin Substances 0.000 abstract 1
- 229940088594 vitamin Drugs 0.000 abstract 1
- 229930003231 vitamin Natural products 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/885—Streptococcus
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
La presente invención caracteriza un medio que permite detección de microbios enterococos en una muestra de líquido biológico cuya procedenciaexcluye aquellas tomadas de seres humanos y animales superiores o ambiental, dentro de tan poco c omo 24horas. El medio actual es distinto dado que losmedios anteriores en los cuales se requería de 48 a 72 horas para que se obtenga una prueba del resultado para Enterococos. El medio también permitecuantificar la cantidad de Enterococos pr esente enuna muestra y poder usar métodos de resguardo rápidos. El medio contiene una cantidad efectiva devitaminas, aminoácidos, ingredientes salinos y oligo elementos operables para permitir viabilidad y reproducción de Enterococos en la presencia deunindicador nutriente. El indicador nutriente se provee en una cantidad suficiente para permitir una senal característica perceptible producida en elmedio por crecimiento de Enterococos. El medio además contiene cantidades e fectivas deagentes selectivos que son activos para impedir o inhibir elcrecimiento de microorganismo no objetivos (por ej. no Enterococos). Otro aspecto de la invención es un método para detectar la presencia o ausencia deun microbio determina do en unamuestra de líquido ambiental o biológico, exceptuando aquellas que provienen de seres humanos y animales superiores,preferentemente que incluye la etapa de calentar la muestra a la temperatura de incubación en una incubadora líquida después deagregar el medio específico almicrobio, preferentemente la temperatura de incubación es de aproximadamente 35°C.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/335,149 US5620865A (en) | 1994-11-04 | 1994-11-04 | Medium for detecting Enterococci in a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR000061A1 true AR000061A1 (es) | 1997-05-21 |
Family
ID=23310484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AR33409095A AR000061A1 (es) | 1994-11-04 | 1995-11-02 | Medio para detectar dentro de un lapso de 24 horasla presencía o ausencia de enterococos en una mue stra liquida cuya procedencia excluye aquellas tomadas de seres humanos y animales superiores. |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5620865A (es) |
| EP (1) | EP0871854B1 (es) |
| AR (1) | AR000061A1 (es) |
| AT (1) | ATE242476T1 (es) |
| BR (1) | BR9505075A (es) |
| CA (1) | CA2204120C (es) |
| DE (1) | DE69531007T2 (es) |
| ES (1) | ES2201124T3 (es) |
| WO (1) | WO1996015435A2 (es) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5429933A (en) | 1986-06-30 | 1995-07-04 | Edberg; Stephen C. | Detection of first generation environmental sourced microbes in an environmentally-derived sample |
| DE69716644T2 (de) * | 1996-07-26 | 2003-07-03 | Idexx Laboratories, Inc. | Verfahren und medium zum nachweis von vancomycin-resistenten enterococcus |
| US5837482A (en) * | 1997-01-23 | 1998-11-17 | Minnesota Mining And Manufacturing Company | Culture medium and methods for detecting staphylococci |
| US6391578B2 (en) | 1997-04-09 | 2002-05-21 | 3M Innovative Properties Company | Method and devices for partitioning biological sample liquids into microvolumes |
| US6696286B1 (en) | 1997-04-09 | 2004-02-24 | 3M Innovative Properties Company | Method and devices for detecting and enumerating microorganisms |
| US6984499B2 (en) | 1997-10-02 | 2006-01-10 | Idexx Laboratories, Inc. | Method and apparatus for concurrently detecting pathogenic organisms and antimicrobial susceptibility |
| GB2334100B (en) * | 1998-02-10 | 2000-03-22 | Praill Price Richardson Ltd | Chromogenic substrates, media, and methods for the detection of salmonella spp |
| US5968762A (en) * | 1998-03-19 | 1999-10-19 | The University Of Connecticut | Method for detecting bacteria in a sample |
| US5972641A (en) * | 1998-08-28 | 1999-10-26 | Colifast Systems Asa | Rapid coliform detection system |
| US6511819B2 (en) | 1998-08-28 | 2003-01-28 | Nye Colifast As | Rapid coliform detection system |
| US6174699B1 (en) | 1999-03-09 | 2001-01-16 | 3M Innovative Properties Company | Disc assay device with inoculation pad and methods of use |
| US6656917B1 (en) | 1999-06-30 | 2003-12-02 | Marker Gene Technologies, Inc. | Compositions and methods for targeted enzymatic release of cell regulatory compounds |
| US8716558B2 (en) | 1999-06-30 | 2014-05-06 | Marker Gene Technologies, Inc. | Method of altering glycosylation of proteins in response to nojirimycin glucuronide in a plant cell expressing glucuronidase |
| US6402941B1 (en) * | 2000-02-07 | 2002-06-11 | Wastewater Biological Solutions, Corp | Apparatus for biological treatment of environmental contaminants and waste |
| FR2826019B1 (fr) * | 2001-06-13 | 2003-09-26 | Alain Rambach | Milieu de culture pour la detection et/ou la discrimination des enterocoques et procede de mise en oeuvre |
| US20030138906A1 (en) * | 2001-11-05 | 2003-07-24 | Ingun Tryland | Fluorescence test for measuring heterotrophic bacteria in water |
| US7811783B2 (en) | 2001-12-14 | 2010-10-12 | Colifast As | Rapid method of detection and enumeration of sulfide-producing bacteria in food products |
| US6632632B1 (en) * | 2001-12-14 | 2003-10-14 | Colifast As | Rapid method of detection and enumeration of sulfide-producing bacteria in food products |
| CA2473079A1 (en) * | 2002-01-10 | 2003-12-24 | Idexx Laboratories, Inc. | Methods and devices for the detection of pathogenic microorganisms and their antimicrobial susceptilbility |
| FR2845097B1 (fr) * | 2002-10-01 | 2006-06-16 | Metis Biotechnologies | Procede de detection et de comptage de microorganismes dans un echantillon |
| CU23302A1 (es) | 2003-01-10 | 2008-07-24 | Ct Nac Biopreparados | Medio de cultivo selectivo para el aislamiento y detecciã"n de especies del gã0/00nero streptococcus |
| JP2005130768A (ja) * | 2003-10-30 | 2005-05-26 | Kanto Chem Co Inc | 腸球菌検出用培地 |
| CN101061232A (zh) | 2004-06-23 | 2007-10-24 | 罗斯玛丽·凯瑟琳·卡梅伦·莎平 | 微生物检测仪器的改进及其使用方法 |
| JP4925590B2 (ja) * | 2004-08-20 | 2012-04-25 | デンカ生研株式会社 | 乳酸菌用培地 |
| FR2881755B1 (fr) | 2005-02-10 | 2012-11-30 | Biomerieux Sa | Milieux pour la detection specifique de micro-organismes resistants |
| FR2903421B1 (fr) * | 2006-07-10 | 2008-10-03 | Alain Rambach | Milieu de culture solide pour la detection et/ou la discrimination au niveau de l'espece des enterocoques resistants aux glycopeptides |
| JP5118336B2 (ja) * | 2006-11-28 | 2013-01-16 | 日水製薬株式会社 | 腸球菌検出用培地 |
| ES2645455T3 (es) * | 2006-12-19 | 2017-12-05 | Becton, Dickinson And Company | Medio cromogénico para detección e identificación de enterococos resistentes a vancomicina y procedimiento asociado |
| EP2062978A1 (en) | 2007-11-23 | 2009-05-27 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | System for detecting microbial contamination |
| GB0820398D0 (en) * | 2008-11-07 | 2008-12-17 | Oxoid Ltd | Semi-opaque medium for culture of microorganisms |
| WO2017013673A1 (en) | 2015-07-21 | 2017-01-26 | M/S Xcellence In Bio Innovation And Technologies Pvt. Ltd. | An invention relating to microbiological testing apparatus |
| CN113832212A (zh) * | 2021-09-10 | 2021-12-24 | 奎泰斯特(上海)科技有限公司 | 水中肠球菌酶底物法测定剂及其制备方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3206317A (en) * | 1961-06-15 | 1965-09-14 | Golber Leonard Mark | Food spoilage indicator |
| US3496066A (en) * | 1966-03-29 | 1970-02-17 | Boehringer & Soehne Gmbh | Diagnostic aids for use in the detection of bacteria in biological and other fluids |
| US4288543A (en) * | 1977-01-28 | 1981-09-08 | Pfizer Inc. | Method and apparatus for identifying microorganisms |
| FR2405301A1 (fr) * | 1977-10-04 | 1979-05-04 | Api Labor | Substrats et procede permettant l'identification rapide de bacteries du genre streptococcus |
| US4235964A (en) * | 1978-09-28 | 1980-11-25 | Bochner Barry R | Method for testing and identifying microorganisms |
| JPS5817598B2 (ja) * | 1979-10-31 | 1983-04-08 | 味の素株式会社 | 微生物の迅速検出方法 |
| US4925789A (en) * | 1986-06-30 | 1990-05-15 | Edberg Stephen C | Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material |
| US4912036A (en) * | 1986-09-24 | 1990-03-27 | Eastman Kodak Company | Rapid differentiation of bacteria using cyclic polypeptide antibiotics |
| US5164301A (en) * | 1990-06-22 | 1992-11-17 | Difco Laboratories | Process and kit for detecting microbial metabolism |
| FR2696476B1 (fr) * | 1992-10-07 | 1994-12-16 | Alain Rambach | Nouveau milieu de culture pour la mise en évidence de E. coli et procédé pour son utilisation. |
| US5464755A (en) * | 1994-04-29 | 1995-11-07 | Biolog, Inc. | Microbiological medium and method of assay |
-
1994
- 1994-11-04 US US08/335,149 patent/US5620865A/en not_active Expired - Lifetime
-
1995
- 1995-10-23 ES ES95938833T patent/ES2201124T3/es not_active Expired - Lifetime
- 1995-10-23 WO PCT/US1995/013579 patent/WO1996015435A2/en not_active Ceased
- 1995-10-23 AT AT95938833T patent/ATE242476T1/de not_active IP Right Cessation
- 1995-10-23 DE DE69531007T patent/DE69531007T2/de not_active Expired - Lifetime
- 1995-10-23 CA CA002204120A patent/CA2204120C/en not_active Expired - Fee Related
- 1995-10-23 EP EP95938833A patent/EP0871854B1/en not_active Expired - Lifetime
- 1995-11-02 AR AR33409095A patent/AR000061A1/es active IP Right Grant
- 1995-11-03 BR BR9505075A patent/BR9505075A/pt not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2204120C (en) | 2010-01-19 |
| BR9505075A (pt) | 1997-10-21 |
| EP0871854A2 (en) | 1998-10-21 |
| WO1996015435A2 (en) | 1996-05-23 |
| DE69531007D1 (de) | 2003-07-10 |
| DE69531007T2 (de) | 2004-04-01 |
| ES2201124T3 (es) | 2004-03-16 |
| EP0871854B1 (en) | 2003-06-04 |
| WO1996015435A3 (en) | 1996-08-15 |
| ATE242476T1 (de) | 2003-06-15 |
| CA2204120A1 (en) | 1996-05-23 |
| US5620865A (en) | 1997-04-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant, registration |