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AR000060A1 - Medio especifico para un microbio determinado que permite la deteccion de la presencia o ausencia dedicho microbio en un liquido ambiental o biologic o cuya procedencia excluye aquellos tomados de seres humanos y animales superiores. - Google Patents

Medio especifico para un microbio determinado que permite la deteccion de la presencia o ausencia dedicho microbio en un liquido ambiental o biologic o cuya procedencia excluye aquellos tomados de seres humanos y animales superiores.

Info

Publication number
AR000060A1
AR000060A1 AR33408995A AR33408995A AR000060A1 AR 000060 A1 AR000060 A1 AR 000060A1 AR 33408995 A AR33408995 A AR 33408995A AR 33408995 A AR33408995 A AR 33408995A AR 000060 A1 AR000060 A1 AR 000060A1
Authority
AR
Argentina
Prior art keywords
microbe
hours
medium
absence
environmental
Prior art date
Application number
AR33408995A
Other languages
English (en)
Inventor
E Ehrenfeld
C Fricker
Original Assignee
Idexx Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/423,134 external-priority patent/US5610029A/en
Application filed by Idexx Lab Inc filed Critical Idexx Lab Inc
Publication of AR000060A1 publication Critical patent/AR000060A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención caracteriza un medio que permite detección de la presencia o ausencia de un microbio determinado en una muestra líquidabiológica cuya procedencia excluye aquellas tomadas de seres humanos y animales superiores o ambiental dentro de tan poco 18 a 24 horas.Por ejemplo,Escherichiacoli y otros coliformes pueden detectarse dentro de aprox.18 horas desde el comienzo del período de incubación con gran especificidad.Así, el medio actuales distinto de medios anteriores en los cuales aprox.24 horas son requeridas p/ obtener un resultado de prueba p/E.coli.Tal medio puede hacerse usando cant.variables de ambos nutrientes e indicadores de crecimiento. Sin embargo,la inv. provee aminoácidos, vitaminas e ingrediente elementales en cant.mayores que lasque anteriormente se usaron. Otros aspectos de los medios de esta invención incluyen el uso de piruvato de sodio p/ ayudar la recuperación de las bacteriaslastimadas metabólicamente, la utilización de un período termo-equilibrado, el uso de menos cloruro de sodio,y la provisión de otros metabolitos en cantidadesconvenientes. El medio contiene cant. efectivas de vitaminas, aminoácidos, oligoelementos y sales siendo operable p/soportar la reproducción y viabilidadde apoyo del microbio determinado en la presencia de un indicador nutriente. La inv. también caracteriza un método p/detectar la presencia o ausencia de unmicrobio determinado en una muestra líquida ambiental o biológica dentro de 24 horas.Escherichia coli y otros coliformes pueden ser detectados utilizando estemétodo dentro de aprox.solo 18 horas.Este método se caracteriza 1s por mezclar la muestra con el medio de la inv., calentar la mezcla a una temperatura deincubación, pref.35°C, poraprox.20 minutos.La muestra se transfiere entonces a una incubadora de aire, preferentemente a 35°C por aprox. 18 horas.
AR33408995A 1994-11-04 1995-11-02 Medio especifico para un microbio determinado que permite la deteccion de la presencia o ausencia dedicho microbio en un liquido ambiental o biologic o cuya procedencia excluye aquellos tomados de seres humanos y animales superiores. AR000060A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33478894A 1994-11-04 1994-11-04
US08/423,134 US5610029A (en) 1994-11-04 1995-04-18 Medium for detecting target microbes in a sample

Publications (1)

Publication Number Publication Date
AR000060A1 true AR000060A1 (es) 1997-05-21

Family

ID=26989385

Family Applications (1)

Application Number Title Priority Date Filing Date
AR33408995A AR000060A1 (es) 1994-11-04 1995-11-02 Medio especifico para un microbio determinado que permite la deteccion de la presencia o ausencia dedicho microbio en un liquido ambiental o biologic o cuya procedencia excluye aquellos tomados de seres humanos y animales superiores.

Country Status (9)

Country Link
EP (3) EP1403378B1 (es)
JP (2) JPH10509031A (es)
AR (1) AR000060A1 (es)
AT (2) ATE387504T1 (es)
BR (1) BR9505076A (es)
CA (1) CA2204121C (es)
DK (1) DK1403378T3 (es)
ES (1) ES2298457T3 (es)
WO (1) WO1996014432A2 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9617495D0 (en) * 1996-08-21 1996-10-02 Univ Lincolnshire & Humberside Method of and growth medium for detecting micro-organisms in a sample
US5837482A (en) * 1997-01-23 1998-11-17 Minnesota Mining And Manufacturing Company Culture medium and methods for detecting staphylococci
US6391578B2 (en) 1997-04-09 2002-05-21 3M Innovative Properties Company Method and devices for partitioning biological sample liquids into microvolumes
US6696286B1 (en) 1997-04-09 2004-02-24 3M Innovative Properties Company Method and devices for detecting and enumerating microorganisms
US6174699B1 (en) 1999-03-09 2001-01-16 3M Innovative Properties Company Disc assay device with inoculation pad and methods of use
JP4571259B2 (ja) * 1999-12-24 2010-10-27 廣幸 小川 微生物検査用培地、微生物検査具および微生物数検査方法
FR2845097B1 (fr) 2002-10-01 2006-06-16 Metis Biotechnologies Procede de detection et de comptage de microorganismes dans un echantillon
JP2006025608A (ja) * 2004-07-12 2006-02-02 Chisso Corp 微生物培地
CN114174527B (zh) 2019-05-31 2025-03-28 I·A·路易斯 微生物的代谢组学表征
CN113398306B (zh) * 2021-06-30 2023-06-30 山东新华医疗器械股份有限公司 用于灭菌效果监测的极速型生物指示物及使用方法
EP4575495B1 (en) * 2023-12-22 2025-09-24 Joe Guerin Method and system for the detection and/or quantitation of microorganisms

Family Cites Families (13)

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Publication number Priority date Publication date Assignee Title
US4088746A (en) * 1976-11-11 1978-05-09 Bio-Rad Laboratories, Inc. Radioimmunoassay for thyroid-stimulating hormone (TSH)
IL55816A (en) * 1978-10-30 1982-04-30 Ames Yissum Ltd Method for simultaneous immunoassay of several different antibodies and a kit therefor
JPS5817598B2 (ja) * 1979-10-31 1983-04-08 味の素株式会社 微生物の迅速検出方法
DE3266735D1 (en) * 1981-02-18 1985-11-14 Eisai Co Ltd An enzyme immuno-assay for simultaneously measuring a plurality of samples and test vessel for carrying out this method
US4925789A (en) * 1986-06-30 1990-05-15 Edberg Stephen C Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material
US4888287A (en) * 1986-09-24 1989-12-19 Eastman Kodak Company Rapid differentiation of fungi from bacteria using polyene antibiotics
EP0347435A4 (en) * 1987-09-04 1991-11-21 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing soluble t4 proteins
US5518894A (en) * 1987-11-05 1996-05-21 Berg; James D. Rapid coliform detection system
AU2602488A (en) * 1987-11-05 1989-06-01 James D. Berg Rapid process for detecting pathogenic microorganisms
US5354663A (en) * 1988-05-04 1994-10-11 Charm Sciences, Inc. Microbial inhibition test kit and method
IE65282B1 (en) * 1990-04-12 1995-10-18 Becton Dickinson Co Identifying microorganisms by measuring enzymatic activity in the presence of enzyme activity affecting agents
CA2027536C (en) * 1990-05-14 1993-02-16 George Chang Method for determination of e.coli in water
FR2671100B1 (fr) * 1990-12-28 1993-03-05 Bio Merieux Procede d'analyse bacteriologique, et milieu de detection des bacteries genre salmonella.

Also Published As

Publication number Publication date
JPH10509031A (ja) 1998-09-08
CA2204121A1 (en) 1996-05-17
WO1996014432A2 (en) 1996-05-17
DK1403378T3 (da) 2008-06-23
EP1528106A1 (en) 2005-05-04
EP0789779A2 (en) 1997-08-20
MX9703316A (es) 1998-07-31
CA2204121C (en) 2010-12-21
ATE393236T1 (de) 2008-05-15
ES2298457T3 (es) 2008-05-16
EP1528106B1 (en) 2008-04-23
ATE387504T1 (de) 2008-03-15
EP1403378A2 (en) 2004-03-31
EP1403378B1 (en) 2008-02-27
JP2004089196A (ja) 2004-03-25
EP1403378A3 (en) 2004-12-01
BR9505076A (pt) 1997-10-21
WO1996014432A3 (en) 1996-10-24

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