AP1244A - Meta-azacyclic amino benzoic acid compounds and derivatives thereof being integrin antagonists. - Google Patents

Abstract

The present invention is directed to compounds of formula (1)and pharmaceutically acceptable salts and isomers thereof use ful as ... integrin antagonists.

Description

META-AZA CYCLIC AMINO BENZOIC ACID COMPOUNDS AND DERIVATIVES THEREOF BEING INTGRIN ANTAGONISTS

Field of the Invention

The present invention relates to pharmaceutical agents (compounds) which are useful as α_νβ₃ integrin antagonists and as such are useful in pharmaceutical compositions and in methods for treating conditions mediated by ayfc by inhibiting or antagonizing ocvfo integrins.

Background of the Invention

Integrins are a group of cell surface glycoproteins which mediate cell adhesion and therefore are useful mediators of cell adhesion interactions which occur during various biological processes. Integrins are heterodimers composed of noncovalently linked a and β polypeptide subunits. Currently eleven different a subunits have been identified and six different β subunits have been identified. The various σ subunits can combine with various β subunits to form distinct integrins.

) The integrin identified as α_νβ₃ (also known as the vitronectin receptor) has been identified as an integrin which plays a role in various conditions or disease states including tumor metastasis, solid tumor growth (neoplasia), osteoporosis, Paget's disease, humoral hypercalcemia of malignancy, angiogenesis, including tumor angiogenesis, retinopathy, including macular degeneration, arthritis, including rheumatoid arthritis, periodontal disease, psoriasis and smooth muscle cell migration (e.g. restenosis). Additionally, it has been found that such agents would be useful as antivirals, antifungals and antimicrobials. Thus, compounds which selectively inhibit or antagonize α_νβ₃ would be beneficial for treating such conditions.

BNSDOCID: <AP __1244Ά

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It has been shown that the α_νβ₃ integrin and other a, containing integrins bind to a number of Arg-Gly-Asp (RGD) containing matrix macromolecules. Compounds containing the RGD sequence mimic extracellular matrix ligands so as to bind to cell surface receptors.

However, it is also known that RGD peptides in general are non-selective for RGD dependent integrins. For example, most RGD peptides which bind to α_νβ₃ also bind to a_v3s. α_νβι and αικ,β₃. Antagonism of platelet anb^ (also known as the fibrinogen receptor) is known to block platelet aggregation in humans. In order to avoid bleeding side-effects when treating the conditions or disease states associated with the integrin α_νβ_3ι it would be beneficial to develop compounds which are selective antagonists of a_vps as opposed to αι»β₃.

Tumor cell invasion occurs by a three step process: 1) tumor cell ΓΧ attachment to extracellular matrix; 2) proteolytic dissolution of the matrix;

and 3) movement of the cells through the dissolved barrier. This process can occur repeatedly and can result in metastases at sites distant from the original tumor.

F—

Seftor et al. (Proc. Natl. Acad. Sci. USA, Vol. 89 (1992) 1557-1561) have shown that the α_νβ₃ integrin has a biological function in melanoma cell invasion. Montgomery et al., (Proc. Natl. Acad. Sci. USA, Vol. 91 (1994) 8856-60) have demonstrated that the integrin α _νβ₃ expressed on human melanoma cells promotes a survival signal, protecting the cells from apoptosis. Mediation of the tumor cell metastatic pathway by interference with the α_νβ₃ integrin cell adhesion receptor to impede tumor metastasis would be beneficial.

Brooks et al. (Cell. Vol. 79 (1994) 1157-1164) have demonstrated that antagonists of α_νβ₃ provide a therapeutic approach for the treatment of neoplasia (inhibition of solid tumor growth) since systemic administration of α»β₃ antagonists causes dramatic regression of various histologically distinct human tumors.

The adhesion receptor integrin α_νβ₃ was identified as a marker of angiogenic blood vessels in chick and man and therefore such receptor

BNSDOCID: <AP_1244A_I_>

APC 0 1244

Ο plays a critical role in angiogenesis or neovascularization. Angiogenesis is characterized by the invasion, migration and proliferation of smooth muscle and endothelial cells. Antagonists of α_νβ₃ inhibit this process by selectively promoting apoptosis of cells in neovasculature. The growth of new blood vessels, or angiogenesis, also contributes to pathological conditions such as diabetic retinopathy and macular degeneration (Adonis et al., Amer. J. Ophthal., Vol. 118, (1994) 445-450) and rheumatoid arthritis (Peacock et al., J. Exp. Med., Vol. 175, (1992), 1135-1138). Therefore, σ_νβ3 antagonists would be useful therapeutic targets for treating such conditions associated with neovascularization (Brooks et al., Science, Vol. 264, (1994), 569-571).

It has been reported that the cell surface receptor α_νβ₃ is the major integrin on osteoclasts responsible for attachment to bone. Osteoclasts cause bone resorption and when such bone resorbing activity exceeds bone forming activity it results in osteoporosis (a loss of bone), which leads to an increased number of bone fractures, incapacitation and increased mortality. Antagonists of α_νβ₃ have been shown to be potent inhibitors of osteoclastic activity both in vitro (Sato et al., J. Cell. Biol., Vol. 111 (1990) 1713-1723) and in vivo [Fisher et al., Endocrinology, Vol. 132 (1993) 1411-1413). Antagonism of α_νβ₃ leads to decreased bone resorption and therefore restores a normal balance of bone forming and resoround bottoming activity. Thus it would be beneficial to provide antagonists of osteoclast α_νβ₃ which are effective inhibitors of bone resorption and therefore are useful in the treatment or prevention of osteoporosis.

The role of the α_νβ₃ integrin in smooth muscle cell migration also makes it a therapeutic target for prevention or inhibition of neointimal hyperplasia which is a leading cause of restenosis after vascular procedures (Choi et al., J. Vase. Surg. Vol. 19(1) (1994) 125-34).

Prevention or inhibition of neointimal hyperplasia by pharmaceutical agents to prevent or inhibit restenosis would be beneficial.

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BNSDOCID: <AP

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White (Current Biology, Vol. 3(9)(1993) 596-599) has reported that adenovirus uses βνβ? for entering host cells. The integrin appears to be required for endocytosis of the virus particle arid may be required for penetration of the viral genome into the host cell cytoplasm. Thus compounds which inhibit α_νβ3 would find usefulness as antiviral agents.

Summary of the Invention

The present invention relates to compound of the following general formula

wherein X and Y are the same or different halo group and pharmaceutically acceptable salts thereof.

The compounds described above can exist in various isomeric forms and all such isomeric forms are meant to be included. Tautomeric forms are also included as well as pharmaceutically acceptable salts of such isomers and tautomers.

More specifically, the present invention relates to the following compounds:

BNSDOCID: <AP_1244A_l_>

APC 0 1244

AP/?/ 00/01893

BNSDOCID; <AP_1244A_I_>

BNSDOCID: <AP_1244A_I_>

APO 0 1244 ο

ΑΡ/Γ/00 / 0 1 8 9 3 wherein R is Η or alkyl;

or pharmaceutically acceptable salts thereof.

It is another object of the invention to provide pharmaceutical compositions comprising compounds described above. Such compounds and compositions are useful in selectively inhibiting or antagonizing the integrin and therefore in another embodiment the present invention relates to a method of selectively inhibiting or antagonizing the α_νβ₃ integrin. The

BNSDOCID: <AP_1244A_J_>

4.

invention further involves treating or inhibiting pathological conditions associated therewith such as osteoporosis, humoral hypercalcemia of malignancy, Paget's disease, tumor metastasis, solid tumor growth (neoplasia), angiogenesis, including tumor angiogenesis, retinopathy including diabetic retinopathy and macular degeneration, arthritis, including rheumatoid arthritis, periodontal disease, psoriasis, smooth muscle cell migration and restenosis in a mammal in need of such treatment. Additionally, such pharmaceutical agents are useful as antiviral agents, and antimicrobials.

Detailed Description

The present invention relates to a class of compounds represented by Formulae I-XVI, described above.

Preferred embodiments of the present invention are compounds of the following formulae

(XVH) (xvm)

BNSDOCID: <AP.

1244A I >

ΑΡνΟ 1244

ΑΡ/Γ/ Ο Ο ' Ο 1 β 9 3 (XXIV)

io (XXV) (XXVI) (XXVH) (XXVHT) ίο

BNSDOCID: <ΑΡ_1244Α_Ι_>

APOο 12*4

£6810/ 0 0 /i/dV

The invention further relates to pharmaceutical compositions 10 containing therapeutically effective amounts of the compounds described above.

The invention also relates to a method of selectively inhibiting or antagonizing the σ_νβ₃ integrin and more specifically relates to a method of inhibiting bone resorption, periodontal disease, osteoporosis, humoral hypercalcemia of malignancy, Paget's disease, tumor metastasis, solid

BNSDOCID: <AP_ ,1244A_I_>

tumor growth (neoplasia), angiogenesis, including tumor angiogenesis, retinopathy including diabetic retinopathy and macular degeneration, arthritis, including rheumatoid arthritis, smooth muscle cell migration and restenosis by administering a therapeutically effective amount of a compound described above to achieve such inhibition together with a pharmaceutically acceptable carrier.

The following is a list of definitions of various terms used herein;

As used herein, the terms alkyl or lower alkyl refer to a straight chain or branched chain hydrocarbon radicals having from about 1 to about 10 carbon atoms, and more preferably 1 to about 6 carbon atoms. Examples of such alkyl radicals are methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, pentyl, neopentyl, hexyl, isohexyl, and the like.

As used herein the term halo or halogen refers to bromo, chloro,

- is or iodo.

^? As used herein the term haloalkyl refers to alkyl groups as defined

..-. above substituted with one or more of the same or different halo groups at ' one or more carbon atom. Examples of haloalkyl groups include .' i trifluoromethyl, dichloroethyl, fluoropropyl and the like.

,-4 20 The term composition as used herein means a product which results from the mixing or combining of more than one element or ingredient.

The term pharmaceutically acceptable carrier, as used herein means a pharmaceutically-acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting a chemical agent.

The term therapeutically effective amount shall mean that amount of drug or pharmaceutical agent that will elicit the biological or medical response of a tissue, system or animal that is being sought by a researcher or clinician.

The following is a list of abbreviations and the corresponding meanings as used interchangeably herein:

BNSDOCID: <AP_1244A_I_>

ο

ΑΡΰ 0 12 4 4 ¹H-NMR = proton nuclear magnetic resonance

AcOH = acetic acid

Ar = argon

CH₃CN = acetonitrile

CHN analysis = carbon/hydrogen/nitrogen elemental analysis CHNCI analysis = carbon/hydrogen/nitrogen/chlorine elemental analysis

CHNS analysis = carbon/hydrogen/nitrogen/sulfur elemental analysis

DI water = deionized water

DMA = Ν,Ν-dimethylacetamide

DMAP = 4-(N,N-dimethylamino)pyridine

DMF = Ν,Ν-dimethylformamide

EDCI = 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride

EtOAc = ethyl acetate

EtOH = ethanol

FAB MS = fast atom bombardment mass spectroscopy g = gram(s)

HOBT = 1-hydroxybenzotriazole hydrate

HPLC = high performance liquid chromatography

IBCF = isobutylchloroformate

KSCN = potassium thiocyanate

L = liter

LiOH = lithium hydroxide

MEM = methoxyethoxymethyl

MEMCI = methoxyethoxymethyl chloride

MeOH = methanol mg = milligram

MgSOj = magnesium sulfate ml = milliliter mL = milliliter

MS = mass spectroscopy

MTBE = methyl tert-butyl ether

N₂ = nitrogen

NaHCO₃ = sodium bicarbonate

NaOH = sodium hydroxide

Na₂SO₄ = sodium sulfate

NMM = N-methylmorpholine

NMP = N-methyl pyrrolidinone

NMR = nuclear magnetic resonance

P2O5 = phosphorous pentoxide

PTSA = para-toluenesulfonic acid

RPHPLC = reverse phase high performance liquid chromatography

RT = room temperature

TFA = trifluoroacetic acid

THF = tetrahydrofuran

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BNSDOCID: <ΑΡ_1 .L>

TMS - trimethyisilyl Δ = heating the reaction mixture

The compounds described above can exist in various isomeric forms and all such isomeric forms are meant to be included. Tautomeric forms are also included as well as pharmaceutically acceptable salts of such isomers and tautomers.

In the structures and formulas herein, a bond drawn across a bond of a ring can be to any available atom on the ring.

io The term pharmaceutically acceptable salt refers to a salt prepared by contacting a compound described above with an acid whose anion is generally considered suitable for human consumption. Examples of pharmacologically acceptable salts include the hydrochloride, ./ hydrobromide, hydroiodide, sulfate, phosphate, acetate, propionate, * is lactate, maleate, malate, succinate, tartrate salts and the like. All of the pharmacologically acceptable salts may be prepared by conventional means. (See Beroe et al.. J Pharm. Sci., 66(1). 1-19 (1977) for additional examples of pharmaceutically acceptable salts.)

For the selective inhibition or antagonism of α_Ϋβ₃ integrins,

A A • i 20 compounds of the present invention may be administered orally, parenterally, or by inhalation spray, or topically in unit dosage formulations containing conventional pharmaceutically acceptable carriers, adjuvants and vehicles. The term parenteral as used herein includes, for example, subcutaneous, intravenous, intramuscular, intrastemal, infusion techniques or intraperitonally.

The compounds of the present invention are administered by any suitable route in the form of a pharmaceutical composition adapted to such a route, and in a dose effective for the treatment intended. Therapeutically effective doses of the compounds required to prevent or arrest the progress of or to treat the medical condition are readily ascertained by one of ordinary skill in the art using preclinical and clinical approaches familiar to the medicinal arts.

BNSDOCID; <AP_1244A_I_>

ΑΡό 0 12 4 4

Accordingly, .the present invention provides a method of treating conditions mediated by selectively inhibiting or antagonizing the α_νβ₃ cell surface receptor which method comprises administering a therapeutically effective amount of a compound selected from the class of compounds described above, wherein one or more compounds is administered in association with one or more non-toxic, pharmaceutically acceptable carriers and/or diluents and/or adjuvants (collectively referred to herein as carrier*' materials) and if desired other active ingredients. More specifically, the present invention provides a method for inhibition of the α_νβ₃ cell surface receptor. Most preferably the present invention provides a method for inhibiting bone resorption, treating osteoporosis, inhibiting humoral hypercalcemia of malignancy, treating Paget's disease, inhibiting tumor metastasis, inhibiting neoplasia (solid tumor growth), inhibiting angiogenesis including tumor angiogenesis, treating diabetic retinopathy and macular degeneration, inhibiting arthritis, psoriasis and periodontal disease, and inhibiting smooth muscle cell migration including restenosis.

Based upon standard laboratory experimental techniques and procedures well known and appreciated by those skilled in the art, as well as comparisons with compounds of known usefulness, the compounds described above can be used in the treatment of patients suffering from the above pathological conditions. One skilled in the art will recognize that selection of the most appropriate compound of the invention is within the ability of one with ordinary skill in the art and will depend on a variety of factors including assessment of results obtained in standard assay and animal models.

Treatment of a patient afflicted with one of the pathological conditions comprises administering to such a patient an amount of compound described above which is therapeutically effective in controlling the condition or in prolonging the survivability of the patient beyond that expected in the absence of such treatment. As used herein, the term inhibition of the condition refers to slowing, interrupting, arresting or stopping the condition and does not necessarily indicate a total elimination

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5» of the condition. It is believed that prolonging the survivability of a patient, beyond being a significant advantageous effect in and of itself, also indicates that the condition is beneficially controlled to some extent.

As stated previously, the compounds of the invention can be used in a variety of biological, prophylactic or therapeutic areas. It is contemplated that these compounds are useful in prevention or treatment of any disease state or condition wherein the α_νβ₃ integrin plays a role.

The dosage regimen for the compounds and/or compositions containing the compounds is based on a variety of factors, including the io type, age, weight, sex and medical condition of the patient; the severity of the condition; the route of administration; and the activity of the particular compound employed. Thus the dosage regimen may vary widely. Dosage levels of the order from about 0.01 mg to about 1000 mg per kilogram of body weight per day are useful in the treatment of the above-indicated conditions, and more preferably from about 0.01 mg to about 100 mg per kg of body weight per day.

The active ingredient administered by injection is formulated as a composition wherein, for example, saline, dextrose or water may be used as a suitable carrier. A suitable daily dose would typically be about 0.01 to

10 mg/kg body weight injected per day in multiple doses depending on the factors listed above.

For administration to a mammal in need of such treatment, the compounds in a therapeutically effective amount are ordinarily combined with one or more adjuvants appropriate to the indicated route of administration. The compounds may be admixed with lactose, sucrose, starch powder, cellulose esters of alkanoic acids, cellulose alkyl esters, talc, stearic acid, magnesium stearate, magnesium oxide, sodium and calcium salts of phosphoric and sulphuric acids, gelatin, acacia, sodium alginate, polyvinylpyrrolidone, and/or polyvinyl alcohol, and tableted or encapsulated for convenient administration. Alternatively, the compounds may be dissolved in water, polyethylene glycol, propylene glycol, ethanol, corn oil, cottonseed oil, peanut oil, sesame oil, benzyl alcohol, sodium

I6

BNSDOCID: <AP

1244A I >

chloride, and/or various buffers. Other adjuvants and modes of administration are well and widely known in the pharmaceutical art..

The pharmaceutical compositions useful in the present invention may be subjected to conventional pharmaceutical operations such as sterilization and/or may contain conventional pharmaceutical adjuvants such as preservatives, stabilizers, wetting agents, emulsifiers, buffers, etc.

The general synthetic sequences for preparing the compounds useful in the present invention are outlined in Schemes Mil. Both an explanation of, and the actual procedures for, the various aspects of the io present invention are described where appropriate. The following Schemes and Examples are intended to be merely illustrative of the present invention, and not limiting thereof in either scope or spirit. Those with skill in the art will readily understand that known variations of the conditions and processes described in the Schemes and Examples can be used to synthesize the compounds of the present invention.

Unless otherwise indicated ail starting materials and equipment employed were commercially available.

ΑΡ/Γ7 00/01893

BNSDOCID: <AP_ 1244A

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WL 'ft; 4

SCHEME 1

η*..

Scheme I illustrates methodology useful for preparing the tetrahydropyrimidinobenzoic acid portion of the present invention which can be coupled to a giy-0-amino acid ester. Briefly, in Scheme 1, 3,510 dihydroxybenzoic acid is converted to 3-amino-5-hydroxy-benzoic acid using the procedure described in Austr. JL Chem,. 34 (6), 1319-24 (1981). The product is reacted with ammonium thiocyanate in hot dilute hydrochloric acid to give 3-thiourea-5-hydroxybenzoic acid after normal work-up. This thiourea intermediate is converted to the S-methyl 15 derivative by reaction with methyl iodide in ethanol at reflux. 1,3-diamino2-hydroxypropane is reacted with this resulting intermediate in hot DMA. Upon cooling precipitate forms and the zwitterionic product is isolated by filtration. The HCI salt may be obtained by lyophilizing from dilute hydrochloric acid. Alternatively, the product may be isolated from the 20 original reaction mixture by removing volatiles and concentrating. The resulting product is taken up in water and pH adjusted to about 5-7 where zwitterionic product precipitates and is isolated by filtration. The HCI salt

IS

BNSDOCID: <AP_1244A_L>

APV 0 12 4 4 may be obtained as previously stated or by simply dissolving in dilute hydrochloric acid and concentrating to a solid and drying.

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-ir*.

SCHEME ΙΑ

cs₂

-- HO

EtOf-VHjO

Δ

S

BNSDOCID: <AP_1244A_I_>

ΑΡν 0 12 4 4

Scheme ΙΑ illustrates methodology useful for preparing the tetrahydropyrimidinobenzoic acid portion of the present invention which can be coupled to a gly-p-amino acid ester. Briefly, in Scheme IA 1,3diamino-2-hydroxypropane is reacted with carbon disulfide in an appropriate solvent such as ethanol - water, refluxed, cooled, hydrochloric acid added, refluxed again, cooled and the product, 5hydroxytetrahydropyrimidine-2-thione harvested by filtration and dried. This cyclic thiourea intermediate is converted to the S-methyl derivative by reaction of thione and methyl iodide in ethanol at reflux. The desired 210 methylthioether-5-hydiOxypyrimidine hydroiodide is readily isolated by removing volatiles at reduced pressure. Thus, 2-methylthioether-5hydroxypyrimidine hydroiodide in methylene chloride : DMA (about 10:1) and an equivalent of triethylamnine are cooled to about ice-bath . temperature and an equivalent of di-tert-butyl dicdrbonate (BOC anhydride) added. Conventional work-up gives the BOC-2methylthioether-5-hydroxypyrimidine as an oil.

3,5-dihydroxybenzoic acid is converted to 3-amino-5-hydroxybenzoic acid using the procedure of Aust. J. Chem., 34 (6), 1319-24 (1981).

The final desired product, 3-hydroxy-5-[(5-hydroxy-1,4,5,6tetrahydro-2-pyrimidinyl)amino)benzoic acid hydrochloride salt, is prepared a by reacting BOC-2-methylthioether-5-hydroxypyrimidine and 3-amino-5hydroxy-benzoic acid in hot DMA. Upon cooling, a precipitate forms and zwitterionic product isolated by filtration. The HCl salt can be obtained by lyophilizing from dilute hydrochloric acid, for example.

APT/ 0 0 / 0 18 9 3

BNSDOCID: <AP_1244A_I_>

*. !

SCHEME II

X, or W-XSuccinimlde or other BOirce OfX

Y and X are halo groups

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APO 0 12 4«

Γν xv

Scheme Π illustrates methodology useful for preparing the ethyl Ngly-amino-3-(3,5-dihalo-2-hydroxy) phenyl propionate portion of the present invention which can be coupled to the tetrahydropyrimidinobenzoic acid moiety. Briefly, 3,5-halo substituted salicylaldehydes may be prepared by direct halogenation as, for example, would be the case where 5-bromosalicylaldehyde is slurried in acetic acid and an equivalent or more of chlorine is added to yield 3-chloro-5-bromo-2-hydroxybenzaldehyde. Some product precipitates and can be recovered by filtration. The remainder may be recovered by diluting the filtrate with water and isolating the precipitate. Combining the solids and drying gives 3-chloro-5-bromo-2hydroxybenzaldehyde. 3-iodo-5-chlorosalicylaldehyde may be prepared by reacting 5-chlorosalicylaldehyde with N-iodosuccinimide in DMF and subjecting the reaction mixture to usual work-up conditions. 3-iodo-5bromosalicylaldehyde may be prepared by reacting 515 bromosalicylaldehyde in acetonitrile with potassium iodide and chloramine T. Work-up gives a material that when treated with hexanes gives the desired 3-iodo-5-chlorosaiicylaldehyde.

Coumarins are readily prepared from salicylaldehydes using a modified Perkin reaction (e.g., Vogel's Textbook of Practical Organic

Chemistry, 5th Ed., 1989, p. 1040,). The halo-substituted coumarins are converted to 3-aminohydrocoumarins (see J.G. Rico. Tett. Let.. 1994. 35, 6599-6602) which are readily opened in acidic alcohol to give 3-amino-3(3,5-halo-2-hydroxy)phenyl propanoic acid esters.

3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters are converted to N-gly-3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters by reaction of Boc-N-gly-N-hydroxysuccinimide to give Boc-N-gly- 3amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters that are converted to HX salts of N-gly- 3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters (wherein X is a halo group) for example, by removal of the BOC-protecting group using HCl in ethanol.

The amino acid compounds used in preparing the compounds of the present invention can be prepared according to the procedures set forth

ΑΡ/Γ/ 0 0 /0 18 9 3

BNSDOCID: <AP_1244A__I_>

herein and below and according to the methodology described and claimed in co-pending USSN Attorney Docket 3076, filed simultaneously herewith and hereby incorporated by reference.

.‘I

BNSDOCID: <AP__1244A I >

ΑΡΰ 0 12 4 4

SCHEME ΙΠ

Y and X are hate groups

AP/?/ 0 0 /0 18 9 3

BNSDOCID: <AP_1244A_I_>

Scheme ΠΙ is illustrative of methodology useful for preparing various compounds of the present invention. 3-Hydroxy-5-[(1,4,5,6tetrahydro-5-hydroxy-2-pyrimidinyl)amino]benzoic add is activated to coupling using known methods. Thus, after dissolving in a suitable solvent such as DMA an equivalent of NMM is added. The reaction mixture is cooled to ice-bath temperatures and IBCF added. To the mixed anhydride intermediate is added the gly-p-amino acid ester and NMM. Upon completion of the reaction the product is purified by prep hplc and the io ester hydrolyzed to the acid by treating with a base, such as LiOH in a suitable solvent (dioxane/water or acetonitrile/water). Alternatively, a suitable acid, such as TFA can be used. The product is isolated by prep hplc or by isolating the zwitterion at pH 5-7 and converting to the desired salt by standard procedures.

BNSDOCID: <AP_1244A_I_>

Preparation of

Step 1

Preparation of .·Γ*Κ

A.PC' 0 12*4

EXAMPLE A

To a 2L round bottom flask fitted with a mechanical stirrer and condenser was added 3, 5-dichlorosalicylaldehyde (200 g, 1.05 mol, 1 equiv.), acetic anhydride (356 g , 3.49 mol) and triethylamine (95.0 g , 0.94 mol, 0.90 equiv.). The reaction solution was heated at reflux overnight. The dark brown reaction mixture was cooled to 50*C and water (1 L) added with stirring. After one hour the mixture was filtered and the filtrate combined with EtOH (1 L). This mixture was heated to 45^eC for one hour, cooled to room temperature, filtered and the solid (fraction A) washed with EtOH (0.5 L). The combined EtOH solutions were concentrated by rotary evaporation to an oil (fraction B). The solid from fraction A was dissolved in methylene chloride (1.5 L) and the resulting solution passed through a pad of silica gel (1300 mL volume). The resulting dark brown solution was concentrated to an oil that was triturated with hexanes (1.3 L) to give a solid that was isolated by filtration and washed (hexanes) to give substantially pure 6,8-dichlorocoumarin (163 g). A further 31 g of product was obtained by treating the oil, fraction B, in a similar fashion; the oil was

AP/?,' 0 0 / 0 18 9 3

BNSDOCID: <AP

244A I >

dissolved in methylene chloride (0.5 L) passed through a silica pad (0.5 L volume) and triturated with hexanes. The total isolated yield was 194 g or 86% yield of the brown solid.

MS and NMR were consistent with the desired structure.

Step 2

Preparation of

io To a 3-neck 2L round bottom flask fitted with a mechanical stirrer was added 6,8-dichlorocoumarin (160 g, 0.74 mol) (prepared in Step 1) and dry THF (375 mL, Aldrich Sure Seal). The resulting mixture was cooled to - 40^eC (dry ice/acetone bath) and lithium bis(trimethylsilyl)amide (0.80 mol, 800 mL of 1M in THF) added while maintaining temperature

-⁵ is below -40 C. After the completion of the addition the cooling bath was - i removed . After 0.5 hour the mixture had warmed to -5’C. The reaction

U, was quenched by addition of a solution of HCl (0.5 L of^!4M in dioxane) in EtOH (1.25 L). The temperature was maintained below O’C overnight. The reaction mixture was concentrated to about one-half its original volume and partitioned between EtOAc (3 L) and water (2L). The organic layer was washed with aqueous HCl (3 x 1L 0.5 N HCl). The pH of the combined aqueous layers was adjusted to about 7 by addition of 10% aqueous NaOH and extracted with methylene chloride (3 x 2L). The combined organic layers were dried (MgSO<), filtered, and 4M HCl in 25 dioxane (210 mL) added with stirring. Upon completion of precipitation the solid was removed by filtration. The filtrate was concentrated to a small volume and methyl t-butyl ether added. The solid obtained was combined with the initially formed solid and the combined product was washed with

BNSDOCID: <AP_1244A_L>

ΑΡν 0 12 4 4 io )

) methyl t-butyl ether, isolated by filtration and dried (vacuum oven over a weekend) to obtain the desired product (172 g, 74% yield).

MS and NMR were consistent with the desired structure.

Step 3

Preparation of ‘COzEt

OH

Cl

Cl

To a flame-dried round bottom flask (0.5 L) equipped with magnetic stir bar was added N-t-Boc-glycine N-hydroxysuccinimide ester (Sigma, 15.0 g, 0.055 mol), dry DMF (Aldrich Sure Seal, 200 mL) and the product from Step 2 (21.67 g, 0.055 mol) under an inert atmosphere (Ar). The reaction mixture was cooled to approximately 0^eC (salt-ice bath) and Nmethylmorpholine (5.58 g, 0.056 mole) and a catalytic amount of DMAP added and the reaction allowed to proceed overnight. The reaction mixture was concentrated to a slush, and partitioned between EtOAc (0.4L) and aqueous base (2 x 0.2 L, aqueous saturated NaHCO₃). The organic layer was washed consecutively with aqueous citric acid (2 x 0.2 L, 10% w/v), again with aqueous sodium bicarbonate (2 x 0.2 L), brine and dried (Na₂SO4). Volatiles were removed under vacuum at 55°C to give an oil (22.5 g, 92% yield) that solidified on standing.

MS and NMR were consistent with the desired structure.

Step 4

Preparation of

Cl

CO₂Et

OH . _HC(

Cl

AP/TZ 0 0 /0 18 9 3

BNSDOCID: <AP.

1244A I >

The product obtained in Step 3 was de-protected to give the amine hydrochloride salt using the following procedure. To the product from Step 3 (14.0 g, 0.032 mole) in a flame-dried round bottom flask (0.1 L) with stir bar was added dry dioxane (40 mL). To this was added 4.0 N HCI in dioxane (2 equiv., 6.32 mL) at 0’C and the reaction allowed to proceed until gas evolution ceased and the reaction was complete. Volatiles were removed under vacuum and the residue triturated with diethyl ether (50 mL). Solids were collected by filtration and washed with ether and dried to give the desired product (12.5 g).

io MS and NMR were consistent with the desired structure.

.1

BNSDOCID: <AP_1244A_l_>

ΑΡύ 0 12 4«

Preparation of

Step 1

Preparation of

HC1

To a suspension of 3-bromo-5-chlorosalicylaldehyde (175.0 g,

I jo 743.2 mmol) in acetic anhydride (280.5 mL, 3.0 mol) was added triethylamine (103.6 mL, 743.2 mmol). The reaction solution was heated at reflux for 4.5 hours. The solution was cooled and concentrated in vacuo. To the brown residue was added absolute ethanol (730 mL). The mixture ί was stored at 0^eC for 14 hours. The brown solid was collected by filtration and washed with cold ethanol. The solid was dried in vacuo to give the desired product (123.0 g, 64% yield). ’H NMR was consistent with the proposed structure.

ΑΡ/Γ7 00/0 1893

Step 2

Preparation of

BNSDOCID: <AP_1244A_I_>

To a suspension of the coumarin (40.0 g, 154.1 mmol) in THF (400 mL) at -76’C was added, dropwise with stirring, lithium bis(trimethylsilyl)amide (154.1 mL of a 1M solution in THF). The addition was completed in 10 minutes. The reaction mixture was then stirred for 5 minutes, warmed to -20^eC and stirred for 15 minutes. To this solution was added acetic acid (9.25 g, 154.1 mmol) in THF (28 mL) over 5 minutes. The mixture was warmed to room temperature and volatiles were removed in vacuo. The residue was dissolved in ether (850 mL), washed with saturated aqueous NaHCO₃ (2 x 100 mL), brine (2 x 40 mL) and dried (MgS0₄). The ether io solution was concentrated to about 160 mL and cooled to 0 “C. To this suspension was added 4M HCI in dioxane (56.3 mL, 225 mmol) and the mixture was stirred at 0’C for 30 minutes. The suspension was filtered and the filter cake washed with ether. The solid was dried in vacuo to give the desired product as the HCI salt, dioxane solvate, (45.0 g). ’H NMR was consistent with the proposed structure.

Step 3

Preparation of

To a suspension of the lactone (142.2 g, 354.5 mmol) in absolute ethanol (533 mL) was added 4M HCI in dioxane (157.8 mL, 631.1 mmol) over .10 minutes. The reaction mixture was stirred at room temperature for 2.5 hours. Volatiles were removed in vacuo. The residue was dissolved in ethyl acetate (450 mL) and the solution kept at 0°C for 15 hours. The tan precipitate was collected by filtration and washed with cold ethyl acetate. The solid was dried in vacuo to give the desired product as the hydrochloride salt (100.4 g, 79% yield). ¹H NMR was consistent with the proposed structure.

BNSDOCID: <AP_1244A_J_>

APO 0 12 4 4

Step 4

Preparation of

0)

To a flame-dried round bottom flask (0.1 L) equipped with magnetic stir bar was added N-t-Boc-glycine N-hydroxysuccinimide ester (Sigma, 2.72 g, 0.010 mol), dry THF (Aldrich Sure Seal, 50 mL) and the product from Step 3 (3.10 g, 0.01 mole, vacuum desiccated overnight over P₂O₅) under an inert atmosphere (Ar). The reaction mixture was cooled to approximately 0^eC (salt-ice bath) and triethylamine (1.01 g, 0.010 mole) was added. The reaction was allowed to proceed overnight. The reaction mixture was concentrated to a semi-solid and worked up in a fashion similar to Example A, Step 3. Volatiles were removed from the organic layer under vacuum at 55’C to give an oil (4 g, 83% yield) that' solidified on standing.

MS and NMR were consistent with the desired structure.

Step 5

Preparation of

ΑΡ/Τ/Ό 0*01993

The product obtained in Step 4 was de-protected to give the amine hydrochloride salt using the following procedure. To the product from Step (4.0 g, 0.0084 mole) in a flame-dried round bottom flask (0.1 L) with stir bar was added dry dioxane (20 mL). To this was added 4.0 N HCI in dioxane (20 mL) and the reaction allowed to proceed until gas evolution

BNSDOCID: <AP

1244A I >

ceased and the reaction was complete (about one hour). Volatiles were removed under vacuum and the residue triturated with diethyl ether (50 mL). Solids were collected by filtration and washed with ether and dried to give a light brown solid (2.7 g, 78% yield).

MS and NMR were consistent with the desired structure.

BNSDOCID: <AP_1244A I >

APC01244

EXAMPLE C

Preparation of h₂n h

N

Tx	^COjEt r0H	HC1
Br^^	Sr

Step 1

To a suspension of 3,5-dibromosalicylaldehyde (100 g, 357 mmol) io in acetic anhydride (164.8 mL, 1.8 moi) was added triethylamine (45 mL,

375 mmol). The reaction solution was heated overnight at reflux under argon. The solution was cooled to room temperature and a solid mass formed. The dark brown reaction mixture was washed with hot hexanes (3 x 300 mL) and aqueous saturated sodium bicarbonate. The resulting solid was dissolved in EtOAc (2L) and washed with water. The organic layer was dried (sodium sulfate) and concentrated to give a brown solid that was collected by filtration. The solid was dried in vacuo to give substantially pure 6,8-dibromocoumarin (94.2 g, 87% yield).

MS and ¹H NMR were consistent with the desired structure.

AP/P/ 0 0/01883

BNSDOCID: <AP_1244A_L?

Step 2

To 6,8-dibromocoumarin (20.0 g, 0.066 mol) (prepared in Step 1) in

THF (100 mL) at -78*C was added dropwise with stirring lithium bis(trimethylsilyl)amide (66 mL of a 1M solution in THF). The addition was completed in 10 minutes. The reaction mixture was thenstirred for 5 minutes, warmed to 0’C and stirred for 15 minutes. To this solution was added acetic acid (3.95 g) over one minute. The mixture was warmed to io room temperature and volatiles were removed in vacuo. The residue was dissolved in hexanes (500 mL), washed with saturated aqueous NaHCOs (2 x 100 mL) and dried (Na₂SO₄). The organic solution was concentrated to give an oil that was immediately taken up in diethyl ether (400 mL) and 4M HCI in dioxane (30 mL) was added with stirring at 0*C for 30 minutes.

Excess HCI was removed in vacuo, the suspension filtered and the filter cake washed with ether. The solid was dried in vacuo to give the desired ^? product as the HCI salt, dioxane solvate (19.9 g).

I MS and ¹H NMR were consistent with the desired structure.

>

Step 3

The lactone prepared in Step 2 above (15 g) was dissolved in absolute ethanol (400 mL) and anhydrous HCI gas was passed through for one minute. The reaction mixture was stirred at room temperature for 2.5 hours. RPHPLC showed complete reaction The volatiles were removed in vacuo to give a dark residue. The residue was triturated with diethyl

JSDOCID: <AP

1244A_ I >

ΑΡ*· ΠΊ 9 U ether (500 mL) and the mixture stirred overnight. The tan precipitate was collected by filtration and washed with diethyl ether. The solid was dried in vacuo to give the desired product as the hydrochloride salt (15.2 g).

MS and ¹H NMR were consistent with the desired structure.

Step 4

To a flame-dried round bottom flask (0.2 L) equipped with magnetic io stir bar was added N-t-Boc-glycine N-hydroxysuccinimide ester (Sigma,

8.1 g, 0.030 mol), dry DMF (Aldrich Sure Seal, 50 mL) and the product of Step 3 (12 g, 0.03 mole, vacuum desiccated overnight over P2O5) under an inert atmosphere (Ar). The reaction mixture was cooled to approximately 0^eC (salt-ice bath) and N-methyl morpholine (3.03 g, 0.030 mole) and catalytic DMAP added. The reaction was allowed to proceed overnight warming to room temperature. The reaction mixture was concentrated to a semi-solid and worked up in a fashion similar to Example A, Step 3. Volatiles were removed from the organic layer under vacuum at 55’C to give an oil (15.7 g, 93% yield) that solidified on standing. .

MS and NMR were consistent with the desired structure.

APT,' 0 0/01893

BNSDOCID: <AP__1244A_I_>

Step 5

HCI

The product obtained in Step A was deprotected to give the amine hydrochloride salt using the following procedure. To the product from Step 4 (13.0 g, 0.0084 mole) in a flame-dried round bottom flask (0.1 L) with stir bar was added dry dioxane (40 mL). To this was added 4.0 N HCI in dioxane (30 mL) and the reaction allowed to proceed until gas evolution ceased and the reaction was complete (about one hour). The volatiles io were removed under vacuum and the residue triturated with diethyl ether (50 mL). Solids were collected by filtration and washed with ether and dried to give a solid (10.6 g, 93% yield).

MS and NMR were consistent with the desired structure.

BNSDOCID: <AP _1244A I >

ΑΡν 0 12 4 4

Preparation of

EXAMPLE D

• HCl

Step 1

Preparation of 3-chloro-5-bromosalicylaldehyde

To a 5L round bottom flask fitted with a mechanical stirrer and gas addition tube was added 5-bromosalicylaldehyde (495 g, 2.46 mol) and io acetic acid at ambient temperature to form a slurry. To this mixture was added chlorine gas at a moderate rate until a slight molar excess of chlorine (163 g, 1.05 mol) had dissolved. After the addition was stopped the reaction allowed to proceed overnight. The solid formed was recovered by filtration and the filtrate diluted into water (2.5L). The mixture was stirred vigorously for 20 minutes, the product collected by filtration and washed with water. The combined solids were vacuum dried to give the desired 3-chloro-5-bromosalicylaldehyde (475 g, 82% yield).

MS and ¹H NMR were consistent with the desired structure.

AP/TV 0 0/ 0 1893 ²⁰ Step 2

Preparation of 6-bromo-8-chldrocoumarin

BNSDOCID: <AP 1244A

I >

To a 5L round bottom flask fitted with a mechanical stirrer and condenser was placed 3-chloro-5-bromosalicylaldehyde (554.1 g, 2.35 mol, 1 equiv.), acetic anhydride (1203g , 11.8 mol, 5 equiv.) and triethylamine (237.4 g , 2.35 mol, 1 equiv.). The reaction solution was heated at reflux (131-141’C) overnight. The dark brown reaction mixture was cooled to 50’C and ice (2 L) added (ice-bath cooling) with stirring. After one hour the mixture was filtered and the filtrate combined with EtOH (1 L). To this mixture was added EtOH (300 mL) and the reaction mixture stirred for one hour. The precipitate that formed was collected by filtration io and washed with water: EtOH (3x1.3 L), vacuum and dried then dried on a fluid-bed drier. The total isolated yield is 563 g or 92%.

MS and ’H NMR were consistent with the desired structure.

Step 3

Preparation of 3-amino-3- (2-hydroxy-3-chloro-5-bromo)phenyl propanoic acid ethyl ester

To a 3-neck 5L round bottom flask fitted with a mechanical stirrer was added 6-bromo-8-chlorocoumarin (300 g, 1.16 mol) (prepared in Step 2) and dry THF (900 mL, Aldrich Sure Seal). The resulting mixture was cooled to less than -45'C (dry ice/acetone bath) and lithium bis (trimethylsilyl)amide (0.80 mol, 800 mL of 1M in THF and 0.6 L in hexanes, 1.2 equivalents) added while maintaining temperature below

-45'C for 0.5 hour. In a separate 5L flask EtOH (2.5 L) and HCI (4 N HCI in dioxane, 1 L) were combined at -15'C. The coumarin reaction was quenched by addition of the cooled HCI / EtOH solution. After 0.5 hour the resulting reaction mixture temperature was -8.3’C. The reaction mixture was kept at 0'C overnight, concentrated to about 2.5 L and partitioned

BNSDOCID: <AP

1244A I >

ΑΡν 0 12 4 4 between EtOAc (3 L) and water (4 L). The organic layer was washed with aqueous HCI (4 x 1.2 L, 0.5 N HCI). The pH of the combined aqueous layers was adjusted to about 8 by addition of 10% aqueous NaOH and extracted with methylene chloride (1x7 L and 3 x 2L). The combined organic layers were dried (MgS0₄, 900 g), filtered, and 4M HCI in dioxane (400 mL) added with stirring. Upon completion of precipitation the solid was removed by filtration. The mixture was concentrated to 2.5 L, hexanes added (2.5 L) and the precipitate isolated by filtration. The filter cake was washed with methylene chloride/hexanes (1 : 2), suction dried and vacuum io' oven dried at 40C to obtain the desired product (251 g, 60% yield).

MS and ¹H NMR were consistent with the desired structure.

Step 5

Preparation of

The above compound was prepared using essentially the same procedure and relative quantities as specified for its isomer in Example B, Step 4.

MS and ¹H NMR were consistent with the desired structure.

ΑΡ/Γ' 00/01893

Step 6

Preparation of

• HCI

4l

BNSDOCID: <AP

1244A I >

This compound was prepared using essentially the same procedure and relative quantities as specified for its isomer in Example B, Step 5.

MS and ¹H NMR were consistent with the desired structure.

La

7»

BNSDOCID: <AP_1244A

I >

AP 0 Ο 12 A A

EXAMPLE E

Preparation of

N-lodosuccinimide (144.0 g, 0.641 mole) was added to a solution of io 5-chlorosalicylaldehyde (100 g, 0.638 mole) in dimethylformamide (400 mL). The reaction mixture was stirred for 2 days at room temperature. Additional N-iodosuccinimide (20.0 g) was added and the stirring was continued for an additional 2 days. The reaction mixture was diluted with ethyl acetate (1L), washed with hydrochloric acid (300 mL, 0.1 N), water (300 mL), sodium thiosulfate (5%, 300 mL). brine (300 mL), dried (MgSO<) and was concentrated to dryness to afford the desired aldehyde (162 g, 90% yield) as a pale yellow solid.

MS and NMR were consistent with the desired structure.

Step 2

Preparation of 6-chloro-8-iodocoumarin

AP/T/ 0 0 /0 18 9 3

BNSDOCID: <AP_1244A_I_>

A mixture of 3-iodo-5-chlorosalicylaldehyde (100 g, 0.354 mole), acetic anhydride (300 mL) and triethylamine (54 mL) was heated at reflux for 18 hours. Upon cooling, the desired coumarin precipitated as a dark brown crystalline material. This was filtered, washed with hexane/ethyl acetate (4:1,200 mL), and was air dried. Yield: 60 g (55%).

MS and ’H NMR were consistent with the desired structure.

io

Step 3

Preparation of (R.S) -4-amino-3,4-dihydro-6-chloro-8-iodocoumarin hydrochloride.

2U

Lithium hexamethyldisilazane (21.62 mL, 1M, 21.62 mmol) was added to a solution of 6-chloro-8-iodocoumarin (6.63 g, 21.62 mmol) in tetrahydrofuran (100 mL) at -78°C. The reaction mixture was stirred at this temperature for 30 minutes, then at 0°C for 1 hour. Acetic acid (1.3 g,

21.62 mmol) was added to the reaction mixture. The reaction mixture was poured into ethyl acetate (300 mL) and saturated sodium, carbonate (200 mL) solution. The organic layer was separated, washed with brine (200 mL), dried (MgS0₄), and was concentrated to afford a residue. The residue was added to anhydrous ether (200 mL) followed by dioxane/HCI (4N, 30 mL) at 0C. The reaction mixture was stirred for 1 hour at room temperature, filtered, and was dried in vacuo to afford the desired product (4.6 g, 59% yield) as a powder. (RPHPLC: Rf 6.8 minutes; Gradient 10% acetonitrile -90% acetonitrile over 15 minutes then to 100% acetonitrile over the next 6 minutes. Both water and acetonitrile contain 0.1% TFA. Vydac C18 protein peptide column, 2 mL/ minutes flow rate, monitored at 254 nm).

MS and ’H NMR were consistent with the desired structure.

BNSDOCID: <AP

1244A I >

ΑΡν Ο 1 2 4 A

Step 4

Preparation of (R, S)-Ethyl 3-amino-3-(5-chloro-2-hydroxy-3-iodo)phenyl propionate hydrochloride.

Hydrogen chloride gas was bubbled into a solution of 4-amino-3,4dihydro-6-chloro-8-iodocoumarin hydrochloride (22.0 g, 61.09 mmol) in ethanol (250 mL) keeping the reaction mixture at 0-10°C till saturation. After 6 hours at reflux, most of the solvent was removed by distillation.

The cooled residue was added to anhydrous ether and was stirred for 2 io hours. The initial gum turned into a crystalline material. The crystalline product was filtered and was dried to afford the desired product (20 g, 81% yield) as a off-white crystalline powder. (Rf 7.52 minutes, conditions as Step 3).

MS and ’H NMR were consistent with the desired structure.

Step 5

Preparation of (R,S)-ethyl 3-(N-BOC-gly)-amino-3-(5-chloro-2-hydroxy-3iodo)phenyl propionate.

AP/P/ 0 0 /0 18 9 3

A mixture of BOC-gly (2.16 g, 12.31 mmol), HOBT (1.67 g, 12.31 yield), EDCI (2.36 g, 12.31 mmol) and DMF (50 mL) was stirred at 0^eC for 1 hour. Ethyl 3-amino-3-(5-chloro-2-hydroxy-3-iodo)propionate hydrochloride (5.0 g, 12.31 mmol) was added to the reaction mixture followed by triethylamine (3.5 mL). The reaction mixture was stirred for 18 hours at room temperature. DMF was removed in vacuo and the residue

BNSDOCID: <AP_1244A__I_>

ο»/ fit, was partitioned between ethyl acetate (300 mL) and sodium bicarbonate (200 mL). The organic layer was washed with hydrochloric acid (1N, 100 mL), brine (200 mL), dried (MgS0<) and was concentrated to afford the desired product as a solid (6 g, 93% yield).

MS and ¹H NMR were consistent with the desired structure.

Step 6

Preparation of (R,S)-ethyl 3-(N-gly)-amino-3-(5-chloro-2-hydroxy-3iodo)phenyl propionate hydrochloride.

Dioxane/HCI (4N, 20 mL) was added to ethyl 3-(N-BOC-gly)-amino3-(5-chloro-2-hydroxy-3-iodo)propionate (6.0 g, 11.39 mmol) at 0”C and was stirred at room temperature for 3 hours. The reaction mixture was concentrated, and concentrated once more after addition of toluene (100 mL). The residue obtained was suspended in ether and was filtered and dried to afford the desired product as a crystalline powder (5.0 g, 95% yield). (RPHPLC : Rf 8.3 minutes, conditions as in Step 3).

MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <ΑΡ.

.1244A I >

ΑΡ ί· Ο 1 2 4 4

EXAMPLE F

Preparation of o

Br

Step 1

Preparation of 3-iodo-5-bromosalicyialdehyde

HCI

To a solution of 5-bromosalicylaldehyde (20.0 g, 0.1 mole) and potassium iodide (17 g , 0.1 mole) in acetonitrile (150 mL) and water (50 mL) in a 500 mL round bottom flask with magnetic stirrer was added chloramine T (23 g, 0.1 mole). The mixture was allowed to react for one hour. The reaction mixture was partitioned between hydrochloric acid (10%, 200 mL) and ethyl acetate. The organic layer was dried (Na₂SO₄), filtered and concentrated in vacuo. To the residue was added hexanes and the reaction mixture heated to 50^eC for 15 minutes. The undissolved material was removed by filtration. The filtrate was concentrated in vacuo to leave canary yellow 3-iodo-5-bromosalicylaldehyde (26 g).

MS and ’H NMR were consistent with the desired structure.

Step 2

AP/?' 00/01893

The above compound was prepared using essentially the same procedure of Example E, Steps 2-6 where in Step 2, an equivalent amount of product from Step 1, 3-iodo-5-bromo-salicylaldehyde, was substituted for 3-iodo-5-chlorosalicylaldehyde.

MS and ’H NMR were consistent with the desired structure.

BNSDOCID: <AP_'

244A__L>

' * 1

	EXAMPLE H Preparation of OH
5	Step 1 Ethanol (375 mL) and deionized water (375 mL) were added to a 2L 3-neck round bottom flask fitted with a mechanical stirrer, Claisen adapter, addition funnel, reflux condenser and thermocouple. 1,3- diamino-2- hydroxypropane (125.04 g, 1.39 mol) (Aldrich) was added to the reaction
10 V*·	flask and stirred to dissolve. Carbon disulfide (84 mL , 1.39 mol) was added in a drop-wise fashion via addition funnel at 25-33’C over a 35 minute period to afford a milky-white mixture. The temperature was maintained with an ice bath. The reaction mixture was refluxed at 73.4’C for two hours to afford a yellow solution. The reaction mixture was cooled
15	with an ice bath to 25’C and concentrated HCI (84 mL) was added in drop- wise fashion while maintaining the temperature at 25-26eC. The reaction mixture wasjefluxed for 21 hours at 78.4’C. The reaction solution was cooled to 2’C and product collected via vacuum filtration. The white solid
20	was washed 3 times with ice bath chilled ethanol: water (1:1) (50 mL) and dried in vacuo at 40’C to afford 5-hydroxytetrahydropyrimidine-2-thione (63.75 g, 34.7% yield) as a white solid. MS and NMR were consistent with the desired structure. Step 2
25	5-Hydroxytetrahydropyrimidine-2-thione (95 g, 0.72 mol) prepared in Step 1, absolute ethanol (570 mL), and methyl iodide (45 mL , 0.72 mol) were added to a 2 L round bottom flask fitted with a mechanical strirrer and thermocouple. The reaction mixture was refluxed at 78’C for 5 hours and then cooled to room temperature. The reaction mixture was concentrated
BNSDOCID: <AP	48 1244A 1 >

APG Ο 12 A * in vacuo to afford a white solid (194.72 g). The white solid was triturated 3 times with ethyl ether (500 mL ) and dried in vacuo to afford 2methyIthioether-5-hydroxypryrimidine hydroiodide (188.22 g, 95.4% yield) as a white solid.

MS and ’H NMR were consistent with the desired structure.

Step 3

2-Methyl thioether-5-hydroxypyrimid ine hydroiodide (150.81g, 0.55 io mol), methylene chloride (530 mL), dimethylacetamide (53 mL) and triethylamine (76.7 mL, 0.55 mol) were added to a 2L 3-neck round bottom flask fitted with reflux condenser, mechanical stirrer and a static atmosphere of nitrogen. The mixture was cooled with an ice bath and ditert -butyl dicarbonate (120.12 g, 0.55 mol) was added at 4’C. The reaction mixture was heated at 42.5’C for 18 hours to afford a light yellow solution. The reaction solution was transferred to a 2L separatory funnel and washed 3 times with Dl water (200 mL), dried with MgSO₄, filtered and concentrated in vacuo to afford Boc-2-methylthioether-5-hydroxypyrimidine (134.6 g, 99.35% yield) as a light yellow viscous oil.

MS and ¹H NMR were consistent with the desired structure.

Step 4

Boc-2-methylthioether-5-hydroxypyrimidine (50.3 g, 0.204 mol), 325 amino-5-hydroxybenzoic acid (Aust. J. Chem. (1981) 34(6), 1319-24) (25.0 g, 0.1625 mole) and 50 mL anhydrous DMA were heated at 100^eC with stirring for 2 days. A slurry precipitate resulted. The reaction was cooled to room temperature and the precipitate was filtered, washed with CH₃CN, then ethyl ether and dried. This solid was slurried in H₂O and acidified with concentrated HCI resulting in a solution. This was frozen and lyophilized to yield the desired product as a white solid (14.4 g).

MS and ¹H NMR were consistent with the desired structure.

AP/?/ 00/01893

BNSDOCID: <AP_1244A_I_>

Preparation of

EXAMPLE I

CC^Et

OH

HCl

Cl

Step 1

Preparation of Reformatsky Reagent

Br-Zn

-CO2-t-Bu

A 4-L flask fitted with a condenser, temperature probe and φ io mechanical stirrer was charged with Zn metal (180.0 g, 2.76 mol,-30 -100 mesh) and THF (1.25 L). While stirring, 1,2-dibromoethane (4.74 mL, 0.05 mol) was added via syringe (alternatively, TMS Cl (0.1 equivalent) at room temperature for one hour can be substituted). After inert gas purge (3 N₂/vacuum cycles) the suspension of zinc in THF was heated to reflux

- > 15 (65’C) and maintained at this temperature for 1 hour. The mixture was cooled to 50‘C before charging tert-butyl bromoacetate (488 g, 369 mL, 2.5 mol) via 50 mL syringe and syringe pump (delivery set to 4.1 mL/minutes) over 1.5 hours. Reaction temperature of 50’ +/- 5*C was maintained throughout the addition. The reaction mixture was allowed to stir at 50’C for one hour after the addition was complete. Subsequently, the mixture was allowed to cool to 25’C and the precipitated product allowed to settle. The THF mother liquor was decanted into a 2-L round bottom flask using a coarse fritted filter stick and partial vacuum transfer (20 mm Hg). This removed about 65% of the THF from the mixture. 125 Methyl-2-pyrrolidinone (NMP, 800 mL) was added and agitation resumed for 5 minutes. The reaction mixture can be filtered to remove any remaining zinc. Analysis indicated a titer of desired Reformatsky reagent

BNSDOCID: <AP

1244A I >

APt-0 124* of 1.57 M with a molar yield of 94%. Alternatively, the solid reagent can be isolated by filtration from the original reaction mixture. The cake can be washed with THF until a white solid is obtained and dried under N₂ to obtain the desired product as a mono THF solvate that may be stored at 20*C (desiccated) for extended periods. Typical recoveries are 85-90%.

Step 2

2A. Preparation of io

Ο 20

Potassium carbonate (powder, oven dried at 100“C under vacuum, 8.82 g, 60 mmoles) was added to a solution of 3,5-dichlorosaiicylaldehyde (11.46 g, 60 moles) in DMF (40 mL) at room temperature to give a bright yellow slurry. MEMCI (neat, 7.64 g, 61 mmoles) was then added while maintaining the bath temperature at 20°C. The mixture was then stirred at 22*C for 6 hours and MEMCI (0.3 g, 2.4 mmoles) was added. The mixture was stirred for another 0.5 hour and the reaction mixture poured into cold water (200 mL) to precipitate the product. The slurry was filtered on a pressure filter and the cake was washed with water (2 x 50mL) and was dried under N₂/vacuum to afford the product (14.94g, 89%) as a off white solid. ¹H NMR (CDCl_3l TMS) 3.37 (s. 3H), 3.54 to 3.56 (m, 2H), 3.91 to

APT/ 0 0/01893

3.93 (m, 2H), 5.30 (s, 2H), 7.63 (d, 1H), 7.73 (d, 1H), 10.30 (s, 1H); ^,3C NMR (CDCI₃, TMS) d (ppm):59.03, 70.11, 99.57, 126.60, 129.57, 130.81

132.07, 135.36, 154.66, 188.30. DSC: 48.24’C (endo 90.51 J/g); Microanalytical: calcd for CuH₁₂CI₂O₄: C: 47.33%; H: 4.33%; Cl: 25.40%;

found:

C: 47.15%; H:4.26%; Cl: 25.16%.

5l

BNSDOCID: <AP.

2B. Preparation of

The product from Step 2A (35.0 g, 0.125 mol) was charged in a 1-L

3-neck round bottom flask fitted with a mechanical stirrer and an addition funnel followed by addition of THF (200 mL).The solution was stirred at 22°C and (S)-phenyiglycinol (17.20 g, 0.125 mol) was then added at once. After 30 minutes at 22*C, MgSO₄ (20 g) was added. The mixture was stirred for 1 hour at 22‘C, and filtered on a coarse fritted filter. The filtrate io was concentrated under reduced pressure. No further purification was performed and the crude imine was used directly in the coupling reaction, Step 2, C.

2C. Preparation of

A 1-L 3-neck round bottom flask fitted with a mechanical stirrer and an addition funnel was charged with the solid reagent produced in Step 1 (91.3 g, 0.275 mol) and NMP (200 mL) under nitrogen. The solution was then cooled to -10^eC and stirred at 350 rpm. A solution of imine (prepared in Step 2B) in NMP was prepared under nitrogen and then added over 20

BNSDOCID: <AP_ 1244A

I >

APO 0 12 4 4 minutes to the above reaction mixture while the temperature was maintained at -5^eC (jacket temperature -10°C). The mixture was stirred for an additional 1.5 hours at -e°C and one hour at -5’C after the addition was complete.. After cooling to -10^eC a mixture of concentrated HCI/saturated solution of NH₄CI (8.1 mL/ 200 mL) was added in 10 minutes. MTBE (200 ml) was added and the mixture was stirred 15 minutes at 23*C at 200 rpm. Stirring was stopped and the layers separated. The aqueous layer was extracted with MTBE (100 mL). The two organic layers were combined, washed successively with a saturated solution of NH4CI (100 mL), water (100 mL) and brine (100 mL). The solution was dried with MgS0₄ (30 g), filtered and concentrated to afford an orange oil (66.3 g) (solidifies in standing) containing the desired product as a single diastereoisomer (confirmed by proton and carbon nmr). A sample was purified for analysis by recrystallization from heptane to afford the product as an off-white solid.

Proton and carbon NMR and IR spectra were consistent with the desired structure. [a]^D25 = + 8.7° (c = 1.057, MeOH). Microanalytical: calcd for C₂5H33CI₂NO₆:

C: 58.77%; H: 6.47%; N: 2.72%; Cl: 13.78% found: C: 58.22%; H: 6.54%; N: 2.70%; Cl: 13.66%.

AP,' 00*01093

Step 3

Preparation of

3A. A solution of the crude ester prepared in Step 2 [17.40 g, 0.033 mole (theory)j, and EtOH (250 mL) was charged to a 1-L 3-neck jacketed reactor. The solution was cooled to 0°C and Pb (0Ac)4 ( 14.63 g, 0.033 mole) was added at once. After 2 hours a 15% solution of NaOH (30 mL)

BNSDOCID: <AP_ 1244A

I >

was added and ethanol was removed under reduced pressure. Another portion of 15% NaOH (100 mL) was added and the mixture extracted with MTBE (2 x 100 mL), washed with H₂0 (2 x 100 mL) and brine (50 mL), dried with Na₂SO_4l filtered on celite and concentrated under reduced pressure to afford an orange oil (12.46 g). The oil was homogeneous by thin layer chromatography (tic) and was used without further purification.

3B. The oil from 3A was diluted with EtOH (30 mL) and paratoluene sulfonic acid (1.3 equiv., 0.043 moie, 8.18 g) was added. The solution io was heated to reflux for 8 hours, cooled to ambient temperature and concentrated under reduced pressure. The residue was treated with THF (20 mL) and heated to reflux to form a solution. The solution was cooled to room temperature and the compound crystallized. Heptane (30 mL) and

S'

THF (10 mL) were added to form a fluid slurry which was filtered. The cake was washed with THF/heptane (40 mL, 1/1) and vacuum dried for two hours in a pressure filter under nitrogen to afford ajwhite solid (7.40 g).

Proton and carbon NMR and IR spectra were consistent with the desired product as substantially a single enantiomer.

Microanalytical: calcd for Ci_BH₂₁CI₂NO₆S, 0.25 C₄H_eO:

C: 48.73%; H: 4.95%; Ν; 2.99%; Cl: 15.14% found: C: 48.91%; H: 4.95%; Ν: 2.90%; Cl:14.95%.

Step 4

Preparation of

BNSDOCID: <AP_1244A_J_>

(λ && 69124«

To a 500 mL round bottom flask equipped with a magnetic stir bar and nitrogen bubbler were charged the free base of the product produced in Step 3 (21.7 g , 0.065 mole), N-t-Boc-glycine N-hydroxysuccinimide ester (17.7 g, 0.065 mole) and DMF (200 mL). The reaction mixture was stirred under nitrogen at room temperature for 3.25 hours and a pale orange solution formed. The reaction mixture was poured into ice-cold ethyl acetate (1.2 L). The organic solution was washed with 1M HCI (250 mL) and then with brine (500 mL), dried (MgS0₄) and concentrated under vacuum to near dryness to obtain an oil that was subsequently dried at ίο 50*Ό to obtain the product as a colorless oil (28.12 g , 99 %). Seed „ crystals were prepared from ethyl acetate/hexanes. The product (about 28

g) was dissolved in ethyl acetate (35 mL) and hexanes (125 mL). The solution was seeded with the seed crystals and precipitate formed. The solids were filtered and dried overnight under vacuum at 55^eC to yield a colorless solid (27.0 g, 95%).

MS and ¹H NMR were consistent with the desired structure.

Step 5

Preparation of

AP,T70.0 Ό 18 9 3

The Boc-protected glycine amide prepared in Step 4 (27.0 g, 0.062 25 mole) was dried overnight over P₂O₅ and NaOH pellets. The solid was dissolved in dioxane (40 mL) and the solution cooled to 0^eC. An equivalent volume of 4N HCI/dioxane (0.062 mole) was added and the reaction was run for 2 hours. At this point the conversion was 80% by

RPHPLC. The reaction mixture was allowed to warm to room temperature

BNSDOCID: <AP_1244A_I_>

n ft over 4 hours. The reaction mixture was concentrated at 40°C to a foam which was triturated with ether (200 mL). The white solid that formed was filtered and dried over P₂Osto yield the desired glycine beta-amino acid ethyl ester compound, as an HCI salt (20.4g, 88.5% isolated yield).

MS and 'H NMR were consistent with the desired structure.

BNSDOCID: <AP_1244A t >

APO 0 12 4 4

EXAMPLE J

Preparation of

Step 1

Preparation of

MEM protected 3-bromo-5-chlorosalicylaldehyde (129.42 g, 0.4 io mol), prepared according to the procedure of Example I, Step 2A. An equivalent amount of 3-bromo-5-chlorosalicylaldehyde was substituted for 3,5-dichlorosalicylaldehyde, which was charged in a 2-L 3-neck round bottom flask fitted with a mechanical stirrer, followed by addition of THF (640 ml) and (S)-phenylglycinol (54.86 g, 0.4 mol). After 30 minutes at

22°C, MgSO₄ (80 g) was added. The mixture was stirred for 2 hours at

22“C, and filtered on a coarse fritted filter. The filtrate was concentrated under reduced pressure to afford a pale yellow oil (180.0 g) containing the desired imine. No further purification was performed and the crude product was used directly in the coupling reaction, Step 2.

Microanalytical: calcd for C^HziBrCINOz

C: 51.54%; H: 4.78%; N: 3.16%; Br: 18.04%; Cl: 8.00% found: C: 50.22%; H: 4.94%; N: 2.93%; Br; 17.15%; Cl:7.56%.

ΑΡ/Γ7 0 0/ 0 18 9 3

BNSDOCID: <AP_1244A__L>

i .4 Λ

Step 2

Preparation of

In a 5-L 3-neck round bottom flask fitted with a mechanical stirrer, the reagent from Example 1, Step 1 (332.0 g, 0.8 mol) was taken up in NMP (660 mL) under nitrogen. The solution was then cooled to -10^eC. A solution of imine from Step 1 in NMP (320 ml) was prepared under nitrogen io and then added over 30 minutes to the above reaction mixture while the

Λ* ** temperature was maintained at -5^eC. The mixture was stirred for an additional hour at -8^eC and at -5^eC for 2 hours after addition was complete and then cooled to-10^eC. A mixture of concentrated HCI/saturated .- solution of NH₄CI (30mL/720 mL) was added over 10 minutes. MTBE (760 ml) was added and the mixture was stirred for 30 minutes at 23^eC. Stirring was stopped and the layers separated. The aqueous layer was extracted with MTBE (320 ml). The organic layers were combined, washed successively with saturated aqueous NH4CI (320 ml), DI water (320 ml) and brine (320 ml). The solution was dried with MgSO₄ (60 g), filtered and concentrated to afford a yellow oil (221.0 g) containing the desired product as a single diastereoisomer as determined by proton NMR.

DSC: 211.80’C (endo. 72.56 J/g). 228.34°C (98.23 J/g);

Microanalytical: calcd for C₂5H₃₃BrCINO₆:

C: 53.72%; H: 5.95%; N: 2.50%; Br: 14.29%; Cl: 6.33% found: C: 52.11%; H: 6.09%; N: 2.34 %; Br: 12.84%; Cl:6.33%.

BNSDOCID: <AP.

1244A I >

AP 'j 01 2 4 4

Step 3

Preparation of

I .s

A solution of crude ester, prepared in Step 2(-111 g), in ethanol (1500 mL) was charged under argon atmosphere to a 3-L 3-neck round bottom flask fitted with a mechanical stirrer. The reaction mixture was cooled to O’C and lead tetraacetate (88.67 g, 0.2 mol) was added in one portion. The reaction mixture was stirred for 3 hours at O’C and then 15% aqueous NaOH (150 mL) was added to the reaction mixture below 5’C. Methanol was removed under reduced pressure on rotavap. Another 150 mL of 15% aqueous NaOH was added and the reaction mixture was extracted with ethyl acetate (3 x 300 mL) and washed with DI water (2 x 100 mL) and brine (2 x 100 mL) and dried over anhydrous MgSO< (30 g).

It was then filtered over celite and concentrated under reduced pressure to give the desired product (103 g) as a red oil.

Step 4

Preparation of

AP/?/ 00/01893

The above compound was prepared according to the procedure employed for Example I, Step 4 and Step 5 by substituting an equivalent amount of the product from Step 3 in Example I, Step 4. MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP_1244A_L>

‘in

EXAMPLE K

Alternate Preparation of the compound of Example J

Step 1 Preparation of

To the product of Example B, Step 3, (50.0 g, 139.2 mmol) and NaHCO₃(33.5 g, 398.3 mmol) was added CH₂CI₂ (500 mL) and water (335 mL). The mixture was stirred at room temperature for 10 minutes. A solution of benzyl chloroformate (38.0 g, 222.8 mmol) in CH₂CI₂ (380 mL) was added over 20 minutes with rapid stirring. After 50 minutes, the reaction mixture was poured into a separatory funnel and the organic layer collected. The aqueous phase was washed with CH₂CI₂ (170 mL). The combined organic layers were dried (MgSO₄) and concentrated in vacuo. The resulting gummy solid was triturated with hexane and collected by filtration. The tan solid was dried in vacuo to give the desired racemic product (61.2 g, 96% yield). This material was subjected to reverse phase HPLC using a chiral column to give each pure enantiomer. The column employed was a Whelk-0 (R,R), 10 micron particle size using a 90 :10 heptane : ethanol mobile phase. Optical purity was determined to be > 98% using analytical hpic using similar column and solvent conditions. ¹H NMR was consistent with the proposed structure.

Step 2

COjEt

OH

Br

BNSDOCID: <AP_1244A

I >

APO 0 1244

To a solution of the compound obtained in Step 1 (48.5 g, 106.2 mmol) in CH₂CI₂ (450 mL) was added trimethyisilyl iodide (25.5 g, 127.4 mmol) in CH₂CI₂ (100 mL) via canula. The orange solution was stirred at room temperature for 1 hour. Methanol (20.6 mL, 509.7 mmol) was added dropwise and the solution stirred for 15 minutes. The reaction solution was concentrated in vacuo to give an orange oil. The residue was dissolved in methyl f-butyl ether (500 mL) and extracted with 1N HCl (318 mL) and water (1 x 200mL, 1 x 100 mL). The aqueous extracts were back washed with MTBE (100 mL)( To the aqueous solution was added solid io NaHCOa(40.1 g, 478 mmol) in small portions. The basified aqueous mixture was extracted with MTBE (1 X 1L, 2 X 200 mL). The combined organic solution was washed with brine and concentrated in vacuo to give the desired product (23.3 g, 68% yield). ’H NMR was consistent with the proposed structure.

Step 3

Preparation of

APT/ 00/01893 ~ To a solution of the product from Step 2 (23.3 g, 72.1 mmol) in DMF (200 mL) was added N-t-Boc-glycine N-hydroxysuccinimide ester (17.9 g,

65.9 mmol). The reaction mixture was stirred at room temperature for 20 hours. The mixture was poured into ethyl acetate (1.2 L) and washed with 1M HCl (2 X 250 mL), saturated aqueous NaHC0₃ solution (2 X 250 mL) and brine (2 X 250 mL). The solution was dried (MgS0₄) and concentrated to give the desired product (32.0 g, 100 % yield).

Anal, calcd for C,8H2₄BrCIN₂0s: C, 45.06; H, 5.04; N, 5.84.

Found: C, 45.17; H, 5.14; N, 6.12.

¹H NMR was consistent with the desired structure.

6i

BNSDOCID: <AP_1244A_I_>

Step 4

To a solution of the product of Step 3 (31.9 g, 66.5 mmol), in 5 absolute ethanol (205 mL) was added an ethanolic HCI solution (111 mL of a 3M solution, 332.4 mmol). The reaction solution was heated at 58’C for 30 minutes. The solution was cooled and concentrated in vacuo. The residue was dissolved in ethyl acetate (250 mL) and stirred at 0‘C for 2 hours. A white precipitate was collected by filtration and washed with cold io ethyl acetate. The solid was dried in vacuo to give the desired product © (23.5 g, 85% yield).

Anal, calcd for C_i3H«BrCIN₂O₄ + 1.0 HCI; C, 37.53; H, 4.12; N, 6.73.

Found: C, 37.29; H, 4.06; N, 6.68.

¹H NMR was consistent with the desired structure.

aL

BNSDOCID: <AP_1244A_I_>

Preparation of

Step 1

Preparation of

APO 0 12 4 4

EXAMPLE L

Potassium carbonate (powder, oven dried at 100^eC under vacuum, io 22.1g, 0.16 moles) was added to a solution of 3-chloro-5bromosalicylaldehyde (35.0 g, 0.15 moles) in DMF (175 ml) at room temperature to give a bright yellow slurry. MEMCI (neat, 25.0 g, 0.2 moles) was then added while maintaining the bath temperature at 20^eC. The mixture was then stirred at 22’C for 6 hours and was poured into DI water is (1200mL) to precipitate the product. The slurry was filtered on a pressure filter and the cake was washed with DI water (2 x 400 mL) and was dried under N₂/vacuum to afford the product (46.Og, 95 % yield) as an off white solid. ¹H NMR (CDCI_X TMS) 3.35 (s, 3H), 3.54 to 3.56 (m, 2H), 3.91 to 3.93 (m, 2H), 5.30 (s, 2H), 7.77 (d, 1H), 7.85 (d, 1H), 10.30 (s, 1H); ¹³C

NMR (CDCI₃, TMS) (ppm):59.05, 70.11, 71.49, 99.50, 117.93, 129.69, 129.78, 132.37, 138.14, 155.12, 188.22. DSC: 48.24’C (endo 90.51 J/g); Microanalytical: calcd for C_nH₁₂BrCIO₄:

C: 40.82%; H: 3.74%; Cl: 10.95%; Br: 24.69%; found: C: 40.64%; H:3.48%; Cl: 10.99%; Br 24.67%.

ΑΡ/Γ' 0 0 / 0 189 3

BNSDOCID: <AP_1244A_I_>

Step 2

Preparation of

The product from Step 1 (32.35 g., 0.1 mol) was charged in a 500 ml

3N round bottom flask fitted with a mechanical stirrer, followed by addition of THF (160 ml) and (S)-phenylglycinol (13.71 g, 0.1 mol). After 30 minutes at 22^eC, MgSO₄ (20 g ) was added. The mixture was stirred for 1 hour at 22°C and filtered on a coarse fritted filter. The filtrate was ' ν' io concentrated under reduce pressure to afford a pale yellow oil (48.0 g) containing the desired imine. No further purification was performed and the crude product was used directly in the next reaction step. Microanalytical: calcd for Ci₉H₂₁BrCINO₄:

C: 51.54%; H: 4.78%; N: 3.16%; Br: 18.04%; Cl: 8.00% found: C: 51.52%; H: 5.02%; N: 2.82%; Br: 16.31%; Cl:7.61%.

Step 3

Preparation of

In a 5L 3N round bottom flask fitted with a mechanical stirrer, reagent from Example 1, Step 1, (332 g, 0.8 mol) was taken up in NMP

8NSDOCID: <AP.

1244A I >

ΑΡθ Π 1 2 4 A (660 mL) under nitrogen. The solution was then cooled to-10C. A solution of imine produced in Step 2, in NMP (320 ml) was prepared under nitrogen and then added over 30 minutes to the above reaction mixture while the temperature was maintained at -5”C. The mixture was stirred for an additional hour after the addition was complete and cooled to -10^eC. A mixture of concentrated HCI/saturated solution of NH₄CI (30mL/720 mL) was added over 10 minutes. MTBE (760 ml) was added and the mixture was stirred for 1 hour at 23’C. Stirring was stopped and the layers were separated. The aqueous layer was extracted with MTBE (320 ml). The io two organic layers were combined, washed successively with a saturated solution of NH₄CI (320 ml), DI water (320 ml) and brine (320 ml). The solution was dried with MgSO< (60 g), filtered and concentrated to afford a yellow oil (226 g) containing the desired product as a single diastereoisomer. DSC: 227.54’C (endo. 61.63 J/g);

Microanalytical: calcd for C₂5H₃3BrCINO₆:

C: 53.72%; H: 5.95%; N: 2.50%; Br: 14.29%; Cl: 6.33% found: C: 53.80%; H: 6.45%; N: 2.23%; Br: 12.85%; Cl:6.12%.

Step 4

Preparation of

£6810/00 --/dV

A solution of crude ester produced in Step 3(-111 g) in ethanol (1500 mL) was charged under nitrogen atmosphere to a 3L 3N round bottom flask fitted with a mechanical stirrer. The reaction mixture was cooled to 0*C and lead tetraacetate (88.67 g, 0.2 mol) was added in one portion. The reaction mixture was stirred for 3 hours at 0’C and then 15%

BNSDOCID: <AP_1244A_l_s fur.

r» »/r aqueous NaOH (150 mL) was added to the reaction mixture below 5’C. The ethanol was removed under reduced pressure on rotavap. Another 600 mL of 15% aqueous NaOH was added and the reaction mixture was extracted with ethyl acetate (2 x 300 mL), MTBE (2 x 200 mL ) and ethyl acetate (2 x 200 mL). The organic layers were combined and washed with DI water (2 x 200 mL) and brine (2 x 100 mL) and dried over anhydrous MgSO< (30 g). The solution was then filtered over celite and concentrated under reduced pressure to give the product as an orange oil (96 g) that was used in the next step without further purification.

io DSC: 233.60’C (endo. 67.85 J/g);

Microanalytical: calcd for C2₄H_2eBrCINO₆:

C: 54.71%; H: 5.54%; N: 2.65%; Br: 15.16%; Cl: 6.72% found: C: 52.12%; H: 5.40%; N: 2.47%; Br: 14.77%; Cl:6.48%.

Step 5

The crude product from Step 4 (-94 g) was taken up in absolute ethanol (180 mL) and para toluenesulfonic acid monohydrate (50.0 g, 0.26 mo!) was added. The reaction mixture was then heated to reflux for 8 hours after which the solvent was removed under reduced pressure. The residual solid was taken up in THF (100 mL) and the THF was then stripped off under reduced pressure. The residue was dissolved in ethyl acetate (500 mL) and cooled to -5°C. The solid was filtered and washed with heptane (2 x 50 mL) to give a white solid. The solid was then air dried to give the desired product as a white solid (38 g) as a single isomer. ¹H

BNSDOCID: <AP_1244A__I_>

APO ο 1 2 A A

NMR (DMSO, TMS) (ppm) 1.12 (t, 3H), 2.29 (s, 3H), 3.0 (m, 2H), 4.05 (q, 2H),4.88 (t, 1H), 7.11 (d, 2H), 7.48 (d, 2H), 7.55 (d, 1H), 7.68 (1H, d), 8.35 (br. s, 3H); ¹³C NMR (DMSO, TMS) (ppm): 13.82, 20.75, 37.13, 45.59, 60.59, 110.63, 122.47, 125.44, 127.87, 128.06, 129.51, 131.95, 137.77,

145.33,150.14,168.98; DSC:69.86°C (end., 406.5 J/g), 165.72’C (end.

62.27 J/g), 211.24’C (exo. 20.56 J/g) Ιβ]%₅« + 4-2’ (c =0.950,MeOH); IR (MIR) (cm-1)2922, 1726, 1621, 1591, 1494, 1471, 1413, 1376, 1324, 1286,1237, 1207;. Microanalytical: calcd for CieH₂iBrCINO₆S:

C: 43.69%; H:4.27%; N: 2.83%; Br 16.15%, Cl: 716%, S: 6.48% io found: C: 43.40%; H:4.24%; N: 2.73%; Br: 16.40%, Cl: 7.20%, S: 6.54%.

Step 6

Preparation of

The above compound was prepared according to the procedures outlined in Example I, Step 4 and Step 5 where an equivalent quantity of the intermediate prepared in Step 5 as the free base is substituted fin

Example 1, Step 4.

MS and ¹H NMR were consistent with the desired structure.

ΑΡ/Γ/00 /0 18 9 3

BNSDOCID: <AP_1244A__l_i

--Γ

281

EXAMPLE M

Preparation of

Step 1 tg5 •J '»

Preparation of 3-lodo-5-chlorosalicylaldehyde

N-lodosuccinimide (144.0 g, 0.641 mole) was added to a solution of

5-chlorosalicylaldehyde (100 g, 0.638 mole) in dimethylformamide (400 mL). The reaction mixture was stirred for 2 days at room temperature. Additional N-iodosuccinimide (20.0 g) was added and stirring was continued for additional 2 days. The reaction mixture was diluted with ethyl acetate (1L), washed with hydrochloric acid (300 mL, 0.1N), water (300 mL), sodium thiosulfate (5%, 300 mL), brine (300 mL), dried (MgSO₄) and was concentrated to dryness to afford the desired aldehyde as a pale yellow solid (162 g, 90% yield).

MS and ¹H NMR were consistent with the desired structure.

Step 2

Preparation of 2-0-(MEM)-3-iodo-5-chlorosalicylaldehyde

Potassium carbonate (41.4 g, 0.30 mole) was added to a solution of

3-iodo-5-chlorosalicylaldehyde (84.74 g, 0.30 mole) in DMF (200 mL) at 20^eC. This resulted in a yellow slurry and MEM-CI (38.2 g, 0.305 mole) was added maintaining the reaction temperature. After 2 hours, additional

MEM-CI (1.5 g) was added. After stirring for 1 hour, the reaction mixture was poured into an ice-water mixture and was stirred. The precipitate formed, was filtered, and was dried in vacuo to afford the desired protected aldehyde. Yield: 95 g (85%)

MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP_1244A_I_>

Α^ύ01244

Step 3

Preparation of

(S)-Phenyl glycinol (15.37 g, 0.112 mole) was added to a solution of 2-0-{MEM)-3-iodo-5-chlorosalicylaldehyde (41.5 g, 0.112 mole) in THF (200 mL) at room temperature. After 1 hour of stirring MgSO< (16 g) was added and the stirring was continued for 2 hours. The reaction mixture io was filtered and the filtrate was concentrated and was dried in vacuo for 2 hours to obtain the desired intermediate imine. A 2-neck round bottomed flask was charged with the Reformatsky reagent from Example I, Step 1, (81.8 g, 0.2464 mole) and N-methylpyrrolidone (300 mL) and was stirred at -10’C. A solution of the imine in N-methylpyrrolidone (100 mL) was slowly added maintaining the temperature at -1 O’C. The mixture was maintained at this temperature for 2 hours and for 1 hour at -5’C. After cooling the reaction mixture to -10*C, a solution of concentrated HCI in saturated ammonium chloride (16 ml/200 mL) was added. Ethyl ether (500 mL) was added and was stirred for 2 hours at room temperature. The ether layer was separated, and the aqueous layer was further extracted with ether (300 mL). The combined ether layers were washed with saturated ammonium chloride (200 mL), water (200 mL), brine (200 mL), dried (MgSO₄) and concentrated to afford an oil (61.0 g, 90% yield). ¹H NMR indicated that the desired structure was substantially one diastereomer and MS was consistent with the desired structure.

ΑΡ/Γ7 0 0 / 0 18 9 3

BNSDOCID: <AP_1244A_I_>

it

Step 4

Preparation of

SO₃H δ

ch₃ .4

A solution of the crude ester produced in Step 3 (48.85 g, 80.61 mmol) was dissolved in ethanol (500 mL) and was cooled to 0°C. Lead tetraacetate (35.71 g, 80.61 mmol) was added. After 3 hours, 15 % solution of NaOH (73 mL) was added to the reaction mixture. Most of the ethanol was removed under reduced pressure. To the residue was added jo a 15% solution of NaOH (200 mL) and which was then extracted with ether (400 mL). The ether layer was washed with water (100 mL), brine (100

I mL), dried and was concentrated to afford an orange oil. The oil was dissolved in ethanol (100 mL) and para-toluenesulfonic acid (19.9 g) Was added. The solution was heated at reflux for 8 hours and was concentrated under reduced pressure. The residue was diluted with THF (60 mL) and was heated at reflux and was cooled. The precipitate was filtered, washed with hexane/THF (300 mL, 1:1 )and dried to afford the desired product.

MS and ’H NMR were consistent with the desired structure.

Step 5

S - Ethyl 3-(N-BOC-gly)-amino-3-(S)-(5-chloro-2-hydroxy-3-iodo)phenyl propionate

To a mixture of BOC-gly-OSu (9.4 g, 34.51 mmol), ethyl 3-(S)25 amino-3-(5-chloro-2-hydroxy-3-iodo) propionate PTSA salt (17.0 g, 31.38 mmol) in DMF (200 mL) was added triethylamine (4.8 mL). The reaction mixture was stirred for 18 hours at room temperature. The DMF was removed in vacuo and the residue was partitioned between ethyl acetate

BNSDOCID: <AP.

1244A I >

ΑΡθ 0 1244 • (600 mL) and diluted hydrochloric acid (100 mL). The organic layer was washed with sodium bicarbonate (200 mL), brine (200 mL), dried (MgSO₄) and was concentrated to afford of the desired product as a solid (14.2 g, 86% yield).

MS and ¹H NMR were consistent with the desired structure.

Step 6

S - Ethyl 3-(N-gly)-amino-3-(5-chloro-2-hydroxy-3-iodo)phenyl propionate io hydrochloride

Dioxane/HCI (4N, 70 mL) was added to ethyl 3-(S)-(N-BOC-gly)amino-3-(5-chloro-2-hydroxy-3-iodo)phenyl propionate (37.20 g, 70.62 mmol) at 0’C and was stirred at room temperature for 3 hours. The reaction mixture was concentrated, and concentrated once more after addition of toluene (100 mL). The residue obtained was suspended in ether, was filtered and dried to afford the desired product as a crystalline powder (32,0 g, 98% yield).

MS and ¹H NMR were consistent with the desired structure.

£6810/00 / J/dV

BNSDOCID: <AP_1244A_I_>

Preparation of

EXAMPLE N

Step 1

Preparation of

The above compound was prepared according to Example I, Step 2A, substituting an equivalent quantity of 2-hydroxy-3,διό dibromobenzaldehyde for 3,5-dichlorosalicylaldehyde.

Yield 88%; Pale yellow solid; m. p. 46-47 °C; R, = 0.6 (EtOAc/Hexane 1:1 v/v); ’H-NMR (CDCI₃) d 3.37 (s, 3H). 3.56 (m, 2H), 3.92 (m, 2H), 5.29 (s.

. ; 2H), 7.91 (d, 1H, J = 2.4 Hz), 7.94 (d, 1H, J = 2.4 Hz), 10.27 (s, 1H); FAB

- ί MS mZz 367 (M*)

/.»

HR-MS calculated for CuH₁₂Br₂O4 367.9083 found 367.9077.

MS and ¹H NMR were consistent with the desired structure.

Step 2

BNSDOCID: <AP_1244A_I_>

APO01244

The above compound was prepared using the procedure of Example I, Step 2B and Step 2C, substituting an equivalent quantity of the compound of Step 1 in Example I, Step 2B.

Yield 90%; Yellow solid; m. p. 57-59 °C; R, = 0.46 (EtOAc/Hexane 1:1 v/v); ¹H-NMR (CDCfe) d 1.45 (s. 9H); 2.1 (br, 1H, exchangeable), 2.51 (d,

1H, J, = 9.9 Hz, J₂ = 15.3 Hz), 2.66 (d, 1H, J, = 4.2 Hz, J₂ = 15.3 Hz), 3.02 (br, 1H, exchangeable), 3.39 (s, 3H), 3.58 - 3.62 (m, 4H), 3.81 (m, 1H), 3.93 (m, 2H), 4.63 (dd, 1H, J = 4.2 Hz), 5.15 (s, 2H), 7.17-7.25 (m, 6H), 7.49 (d, 1H); FAB- MS m/z 602 (M+H)

HR-MS calculated for Ο^Η^ΝΒ^Οβ 602.0753 found 602.0749.

MS and ¹H NMR were consistent with the desired structure.

Step 3

The above compound (p-toluenesulfonate salt) was prepared according to Example I, Step 3 by substituting an equivalent quantity of the product prepared in Step 2 in Example I, Step 3A. Yield 62%; white solid; ¹H-NMR (DMSO-d₆) d 1.09 (t, 3H, J = 7.2 Hz), 2.27 (s. 3H), 2.97(dd, 2H, J, = 3.0 Hz. J₂ = 7.2 Hz). 4.02 (q, 2H, J = 7.2 Hz), 4.87 (t. 1H, J - 7.2 Hz),

7.08 (d, 2H, J = 4.8 Hz), 7.45 (m, 3H), 7.57 (d, 1H, J = 2.4 Hz), 8.2 (br,3H);

FAB-MS m/z 365 (M+H)

HR-MS calculated for ΟπΗμΝΒγ₂Ο₃, 365.9340 found 365.9311.

MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP

1244A I >

MA

Step 4

Preparation of

Br

O

OH • HC!

CO₂Et

Br

The above compound was prepared using the procedure of Example I, Step 4 substituting the compound prepared in Step 3. The resulting BOC protected intermediate, was converted to the desired compound using the procedure of Example 1, Step 5.

MS and ’H NMR were consistent with the desired structure.

_ ¹⁰

X· _ ¹⁰

X· t

Jf

BNSDOCID: <AP.

BNSDOCID: <AP.

APC01244

EXAMPLE P

Preparation of

• HCl

The above compound is prepared according to the procedure of Example I by substituting an equivalent amount of 3-iodo-5bromosalicylaldehyde prepared in Example F, Step 1 for 3,5dichlorosalicylaldehyde in Example 1, Step 2 A.

ΑΡ/Γ/ 0 0 '0 1 8 9 3

BNSDOCID: <AP_

1244A_I_>

EXAMPLE 1 (±) 3-bromo-5-chloro-2-hydroxy-P-[[2-[[[3-hydroxy-5-[(1,4,5,6tetrahydro-5-hydroxypyrimidin-2-yl)amino)phenyl]carbonyl)amino]acetyl]amino]benzenepropanoic acid, trifluroacetate salt

Preparation of

i y*

To the product of Example H (0.4 g, 0.0014 mole), the product of Example B (0.58 g, 0.0014 mole), triethylamine (0.142 g, 0.0014 mole), DMAP (17 mg), and anhydrous DMA, (4 ml) was added EDCI (0.268 g, 0.0014 mole) at ice bath temperature. The reaction was stirred overnight at room temperature. The resulting ester intermediate was isolated by reverse phase preparatory HPLC. To this ester in H₂O (10 ml) and CH₃CN (5 ml) was added LiOH (580 mg, 0.0138 mole). After stirring at room temperature for 1 hour, the pH was lowered to 2 with TFA and the product was purified by reverse phase preparatory HPLC to yield (after lyophilization) the desired product as a white solid (230 mg).

MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP

1244A I >

APOΟ 12 4 4

EXAMPLE 2

Preparation of

The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example A for the product from Example B.

The yield, after lyophilization was 320 mg of as a white solid, io MS and ¹H NMR were consistent with the desired structure.

ΑΡ/ΓΖ 0 0/Q 1893

BNSDOCID: <AP_1244A_I_>

EXAMPLE 3

Preparation of o

The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example F for the product from Example B. The yield (after lyophilization) was 180 mg as a white solid.

io MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP__1244A_l_>

ΑΡΐ 0 124*

EXAMPLE 4

Preparation of

□

The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product of Example D for the product of Example B. The yield (after lyophilization) was 180 mg as a white solid.

MS and ¹H NMR were consistent with the desired structure.

ΑΡ/ΓΌ0 '0 189 3

BNSDOCID: <ΑΡ.

1244A__I_>

EXAMPLE 5

Preparation of

The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example E for the product from Example B. The yield (after lyophilization) was 250 mg as a white solid.

MS and ’H NMR were consistent with the desired structure.

BNSDOCID: <AP_1244A_I_>

APO Ο 12 A 4

EXAMPLE 6

Preparation of

O

The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example C for the product from Example B. The yield (after lyophilization) was 220 mg as a white solid.

io MS and ’H NMR were consistent with the desired product.

ΑΡ/Γ/ 00/01893

BNSDOCID: <AP_1244A_I_>

EXAMPLE 7

Preparation of

To the product from Example H (7.8 g, 0.027 mole) dissolved in anhydrous DMA (50 mL) in a flame dried flask under N₂ and at ice bath temperature was slowly added isobutylchloroformate (3.7 g, 0.027 mole) followed by N-methylmorpholine (2.73 g, 0.027 mole). The solution was io stirred at ice bath temperature for 15 minutes. To the reaction mixture was X then added the product from Example L (10.0 g, 0.024 mole) at ice bath temperature followed by N-methylmorpholine (2.43 g, 0.024 mole).

The reaction was then stirred at room temperature overnight. The resulting ester intermediate was isolated by reverse phase prep HPLC. To the ester in H₂O (60 mL) and CH₃CN (30 mL ) was added LiOH (10 g, 0.238 mole). The reaction mixture was stirred at room temperature for 1 hour. The pH was then lowered to 2 with TFA. The product was purified by reverse phase prep HPLC to yield (after lyophilization) the desired product as a white solid (9.7 g).

MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP_1244A_l_>

)

APO 0 12 4 4

EXAMPLE 8 (S) 3,5-dichloro-2-hydroxy-p-[[2-I((³-hydroxy-5-[(1_l4_l5,6-tetrahydro-5hydroxypyrimidin-2-yl)amino]phenyl]carbonyl]amino]acetyl)amino]benzenepropanoic acid, monohydrochloride monohydrate

Preparation of

CO₂H

OH • HCI

Cl

Step A

To the product from Example H (9.92 g, 0.0345 mole) dissolved in anhydrous DME (200 mL) is added N-methylmorpholine (4.0 mL, 0.0362 mole). The reaction mixture was cooled to -5’C (salt-ice bath). Isobutylchloroformate, IBCF (4.48 mL, 4.713 g, 0.0345 mole) was added over one minute and the reaction mixture stirred at ice bath temperature for 12 minutes. To the reaction mixture was then added the product from Example 1 (11.15 g, 0.030 mole) at ice bath temperature followed by Nmethylmorpholine (4.0 mL, 0.0362 mole).' The reaction mixture was allowed to warm to room temperature and go to completion then concentrated under vacuum at 50^eC to give a dark residue. The residue was dissolved in acetonitrile : H₂O (about 50 mL). The pH was made acidic by addition of a small amount of TFA. The residue was placed on a 10 x 500 cm C-18 (50 u particle size) column and the ester of the desired product isolated. (Solvent program: 100% H₂O + 0.05% TFA to 30:70 H₂O + 0.05% TFA : acetonitrile + 0.05% TFA over 1 hour @100 mL/minute: the solvent program was initiated after the solvent front elutes). Preparatory RPHPLC purification resulted in a white solid (10.5 g) after lyophilization (50%).

AP/T7 0 0 / 0 18 9 3

MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP_'

44A_I_>

Step Β

The product produced in Step A (about 11 g) was dissolved in dioxane : water and the pH of the solution adjusted to approximately 11.5 (pH meter) by the addition of 2.5 N NaOH. The reaction mixture was stirred at room temperature. Periodically, the pH was re-adjusted to > 11 by further addition of base. After 2-3 hours the conversion of ester to acid was deemed complete by RPHPLC. The pH of the reaction mixture was adjusted to about 6 and a viscous oil precipitated from solution. The oil was isolated by decantation and washed with hot water (200 mL). The io resulting aqueous mixture was allowed to cool and the solid was collected by filtration to yield The above compound (2.6 g after lyophilization from HCl solution). The residue, which was a dark viscous oil was treated with hot water to give on cooling a tan powder (4.12 g after lyophilization from HCl solution).

, MS and ¹H NMR were consistent with the desired structure.

BNSDOCID: <AP.

1244A I >

APOΟ 124 A

EXAMPLE 9 (S) 3-bromo-5-chloro-2-hydroxy-3-[[2-[[[3-hydroxy-5-[(1,4,5,6-tetrahydro-5hydroxypyrimidin-2-yl)amino]phenyl]carbonyl)amino]acetylJamino]benzenepropanoic acid, trifluoroacetate salt

Step 1 - Preparation of

To a suspension of the product from Example J (1.0 g, 2.4 mmol), io the product from Example H (0.75 g, 2.6 mmol) and 4dimethylaminopyridine (40 mg) in Ν,Ν-dimethylacetamide (10 mL) was added triethylamine (0.24 g. 2.4 mmol). The mixture was stirred at room temperature for 15 minutes and 1 -(3-dimethylaminopropyl)-3ethylcarbodiimide hydrochloride (0.60 g, 3.1 mmol). The reaction mixture was stirred at room temperature overnight. The mixture was concentrated in vacuo and purified by reverse phase HPLC (starting gradient 90:10 H₂O/TFA:MeCN, retention time 22 minutes) to give the desired product, © (1.6 g, 52% yield).

¹H NMR was consistent with the proposed structure.

Step 2

AP/’*' 0 0 ι 0 18 9 3

BNSDOCID: <AP 1244A I >

To a solution of the ester produced in Step 1 (800 mg, 1.2 mmol) in a 1:4 MeCN:H₂O solution (7 mL) was added lithium hydroxide (148 mg, 6.2 mmol). The reaction mixture was stirred at room temperature for 2 hours. TFA (0.71 mL, 9.2 mmol) was added and the mixture purified by reverse phase HPLC (starting gradient 95:5 H₂O/TFA:MeCN, retention time 24 minutes) to give the desired product (860 mg, 83% yield).

Anal, calcd for C22H₂₃BrCIN₅O7 + 1.7 TFA:

C, 39.18; H, 3.20; N, 8.99.

Found; C, 39.11; H, 3.17; N, 9.07.

io MS and ’H NMR were consistent with the desired structure.

/

Step 3

Preparation of the hydrochloride salt *£· 15 The product of Step 2 was dissolved in a suitable solvent (acetontrile : water) and the solution slowly passed through a Bio-Rad AG2 - 8X (chloride form, 200 - 400 mesh, > 5 equivalents) ion-exchange

7“ column. Lyophilization gives the desired product as an HCI salt.

I

Wtt,

BNSDOCID: <AP.

Ί244Α I >

APC 0 12 4*

EXAMPLE 10

Preparation of

The above compound was prepared using the procedure of Example 8 substituting the product of Example N for the product of Example I in Example 8, Step A. The product was isolated by prep RPHPLC and lyophilized to give the desired product as a TFA salt.

MS and ¹H NMR were consistent with the desired structure.

AP/?/ 0 0/0 189 3 ✓-· ·. ϋ

BNSDOCID: <AP_'

244A_1_>

EXAMPLE 11

Preparation of

The above compound was prepared using essentially the procedures of Example 8 and substituting the product of Example M for the product of Example 1 in Example 8, Step A. The product was isolated io by preparatory RPHPLC and lyophilized to give the desired product as a TFAsalt.

* MS and ’H NMR were consistent with the desired structure.

ί

BNSDOCID: <AP _ 1244A >

EXAMPLE 12

Preparation of

The above compound was prepared using the procedures of Example 8 and substituting the product of Example P for the product of

Example I in Example 8, Step A. The product is isolated by prep RPHPLC and lyophilized to give the desired product as a TFA salt.

BNSDOCID; <AP.

244A I >

EXAMPLE 13

Preparation of

Preparation of 2-0-(MEM)-3,5-diiodosalicylaldehyde

Potassium carbonate (18.5 g, 0.134 mole) was added to a solution of 3,5-diiodosalicylaldehyde (50.0 g, 0.134 mole) in DMF (150 mL) at 20°C. This resulted in a yellow slurry and MEM-CI (15.8 mL, 0.134 mole) io was added maintaining the reaction temperature. After 2 hours, additional ~ MEM-CI (1.5 g) was added. After stirring for a further 1 hour, the reaction mixture was poured into ice-water and stirred. The precipitate formed, was .4 filtered, and dried in vacuo to afford the desired protected aldehyde (61 g, * 99% yield). ⁿH NMR was consistent with the desired product.

¹⁵

Step 2

Preparation of

BNSDOCID: <AP_1244A_I_>

APO 0 12 4 4 (S)-phenyl glycinol (17.9 g, 0.13 mole) was added to a solution of 2O-(MEM)-3,5-diiodosalicylaldehyde (41.5 g, 0.112 mole) in THF (150 mL) at room temperature. After 1 hour of stirring MgS0₄ (20.7 g) was added and the stirring was continued for 2 hours. The reaction mixture was filtered and the filtrate was concentrated and dried in vacuo for 2 hours. A 2-neck round bottomed flask was charged with the Reformatsky reagent (96 g, 0.289 mole) and N-methylpyrrolidone (250 mL) and was stirred at -10°C. A solution of the imine in N-methylpyrrolidone (100 mL) was slowly added maintaining the temperature at -10°C. The mixture was maintained io at this temperature for 2 hours and for 1 hour at -5°C. After cooling the reaction mixture to -10^eC, a solution of concentrated HCl in saturated ammonium chloride (16 ml/200 mL) was added. Ethyl ether (500 mL) was added and the mixture was stirred for 2 hours at room temperature. The ether layer was separated, and the aqueous layer further extracted with ether (300 mL). The combined ether layers were washed with saturated ammonium chloride (200 mL), water (200 mL), brine (200 mL), dried (MgS0₄) and concentrated to afford an oil (90.0 g, 99% yield). NMR indicated desired product and one diastereomer.

Step 3

Preparation of

A solution of the crude ester from Step 2 (14.0 g, 20.1 mmol) was dissolved in ethanol (100 mL) and was cooled to 0°C. Lead tetra acetate (9.20 g, 20.75 mmol) was added in one lot. After 3 hours, 15% solution of

NaOH (73 mL) was added to the reaction mixture. Most of the ethanol was

9)

BNSDOCID: <AP_____1244A_I_>

removed under reduced pressure. The residue was added to a 15% solution of NaOH (200 mL) which was extracted with ether (400 mL). The ether layer was washed with water (100 mL), brine (100 mL), dried and concentrated to afford an orange oil. This was dissolved in ethanol (100 mL) and para-toluenesulfonic acid (6.08 g) was added. The solution was heated at reflux for 8 hours and was concentrated under reduced pressure. The residue was diluted with THF (60 mL), was heated at reflux and was cooled. Upon storage, no precipitate formed. The reaction mixture was concentrated and purified by preparative hplc to afford the amino acid as io its PTSA salt. The solid obtained was dissolved in ethanol and was saturated with HCl gas. The reaction mixture was heated at reflux for 6 hours. The reaction mixture was concentrated to afford the PTSA salt of the desired amino acid (12.47 g).

To a mixture of BOC-gly-OSu (7.48 g, 27.04 mmol), ethyl 3-(S)amino-3-(3,5-diiodo-2-hydroxyphenyl)propionate PTSA salt (12.47g, 27.04 mmol) in DMF (100 mL) was added triethylamine (3.8 mL). The reaction mixture was stirred for 18 hours at room temperature. The DMF was removed in vacuo and the residue partitioned between ethyl acetate (600 mL) and dilute hydrochloric acid (100 mL). The organic layer was washed with sodium bicarbonate (200 mL), brine (200 mL), dried (MgSO4) and concentrated to afford the desired product as a solid (17.0 g, 96% yield).

’H NMR was consistent with the desired product.

BNSDOCID: <AP_1244A_L>

ΑΡΟθ1244

Step 5

Preparation of ethyl 3-(N-gly)-amino-3-(3,5-diiodo-2-hydroxyphenyl)propionate hydrochloride

To dioxane/HCI (4N, 40 mL) was added ethyl 3-(N-BOC-gly)-amino3-(S)-(3,5-diiodo-2-hydroxyphenyl)propionate (17.0 g, 25.97 mmol) at 0°C and the reaction mixture was stirred at room temperature for 3 hours. The reaction mixture was concentrated, and concentrated once more after io addition of toluene (100 mL). The residue obtained was dried to afford the desired product as a crystalline powder (8.0 g, 56% yield). ¹H NMR is consistent with the desired product.

Step 6

A solution of m-(5-hydroxypyrimidino)hippuric acid (3.74 g, 12.98 mmol) in dimethylacetamide (25 mL) was heated until all the material had dissolved. This was then cooled to 0°C and isobutylchloroformate (1.68 mL) was added in one portion followed by N-methylmorpholine (1.45 mL). After 10 minutes, ethyl 3-(N-gly)-amino-3-(3,5-diiodo-2-hydroxyphenyl)20 propionate hydrochloride (6.0 g, 10.82 mmol) was added in one portion followed by N-methylmorpholine (1.45 mL). The reaction mixture was stirred for 18 hours at room temperature. The reaction mixture was concentrated, the residue dissolved in tetrahydrofuran/water (1:1, 20mL), and was chromatographed (reverse phase, 95:5 water: acetonitrile over 60 minutes to 30:70 water: acetonitrile containing 0.1% TFA). The combined fractions were concentrated. The residue was dissolved in acetonitrile water and lithium hydroxide was added until basic. The solution was stirred for 2 hours. The reaction mixture was concentrated and was £6810/00 /J/dV

BNSDOCID: <AP

1244A I >

purified as above by hplc to afford the desired acid as the TFA salt. The TFA salt was converted to the corresponding hydrochloride salt by passing through an ion-exchange column followed by lyophilization. ’H NMR was consistent with the desired product.

BNSDOCID: <AP_1244A_I_>

A.PC 0 1244

EXAMPLES 14-18

The compounds of the formula VII, vm, IX, ΧΙΠ and XIV and isomers thereof, can be prepared according to the methodology disclosed herein substituting the appropriate starting materials and reagents as would be apparent to one with ordinary skill in the art.

AP/T7 0 0 / 0 18 9 3

BNSDOCID: <AP_1244A_I_>

The activity of the compounds of the present invention was tested in the following assays. The results of testing in the assays are tabulated in Table 1.

VITRONECTIN ADHESION ASSAY MATERIALS

Human vitronectin receptor (α_νβ3) was purified from human placenta as previously described [Pytela et al., Methods in Enzvmotogy, 144:475489 (1987)]. Human vitronectin was purified from fresh frozen plasma as io previously described [Yatohgo et al., Cell Structure and Function, 13:281292 (1988)]. Biotinylated human vitronectin was prepared by coupling NHS-biotin from Pierce Chemical Company (Rockford, IL) to purified vitronectin as previously described [Charo et al., J. Biol. Chem,. 266(3):1415-1421 (1991)]. Assay buffer, OPD substrate tablets, and RIA grade BSA were obtained from Sigma (St. Louis, MO). Anti-biotin antibody was obtained from Calbiochem (La Jolla, CA). Linbro microtiter plates were obtained from Flow Labs (McLean, VA). ADP reagent was obtained from Sigma (St. Louis, MO).

20

METHODS

O

Solid Phase Receptor Assays

This assay was essentially the same as previously reported [Niiya et al., Blood. 70:475-483 (1987)]. The purified human vitronectin receptor (α_νβ3) was diluted from stock solutions to 1.0 g/mL in tris-buffered saline containing 1.0 mM Ca⁺⁺, Mg⁺⁺. and Mn⁺⁺, pH 7.4 (TBS⁺⁺⁺). The diluted receptor was immediately transferred to Linbro microtiter plates at 100

L/well (100 ng receptor/well). The plates were sealed and incubated overnight at 4’C to allow the receptor to bind to the wells. All remaining steps were at room temperature. The assay plates were emptied and 200 L

BNSDOCID: <AP__1244A_I_>

APOο 1244

^.81 of 1% RIA grade BSA in TBS⁺⁺⁺ (TBS⁺⁺⁺/BSA) were added to block exposed plastic surfaces. Following a 2 hour incubation, the assay plates were washed with TBS⁺⁺⁺ using a 96 well plate washer. Logarithmic serial dilution of the test compound and controls were made starting at a stock concentration of 2 mM and using 2 nM biotinylated vitronectin in

TBS⁺⁺⁺/BSA as the diluent. This premixing of labeled ligand with test (or control) ligand, and subsequent transfer of 50 L aliquots to the assay plate was carried out with a CETUS Propette robot; the final concentration of the labeled ligand was 1 nM and the highest concentration of test compound io was 1.0 x 10* M. The competition occurred for two hours after which all wells were washed with a plate washer as before. Affinity purified horseradish peroxidase labeled goat anti-biotin antibody was diluted 1:3000 in TBS⁺⁺+/BSA and 125 L were added to each well. After 30 minutes, the plates were washed and incubated with OPD/H₂O substrate in 100 mM/L

Citrate buffer, pH 5.0. The plate was read with a microtiter plate reader at a wavelength of 450 nm and when the maximum-binding control wells reached an absoround bottomance of about 1.0, the final A*» were recorded for analysis. The data were analyzed using a macro written for use with the EXCEL spreadsheet program. The mean, standard deviation, and %CV ' 20 were determined for duplicate concentrations. The mean A<» values were normalized to the mean of four maximum-binding controls (no competitor

O added)(B-MAX). The normalized values were subjected to a four parameter curve fit algorithm [Rodbard et al., Int, Atomic Energy Agency, Vienna, pp 469 (1977)], plotted on a semi-log scale, and the computed concentration corresponding to inhibition of 50% of the maximum binding of biotinylated vitronectin (IC») and corresponding R² was reported for those compounds exhibiting greater than 50% inhibition at the highest concentration tested; otherwise the IC» is reported as being greater than the highest concentration tested. P-[[2-[[5-[(aminoiminomethyl)-amino]-130 oxopentyl]amino]-1-oxoethyl]amino]-3-pyridinepropanoic acid [USSN

08/375,338, Example 1] which is a potent α_νβ₃ antagonist (1C» in the range

3-10 nM) was included on each plate as a positive control.

£6810/00 1J/dV

BNSDOCID: <AP_1244A_L>

ri

PURIFIED llb/llla RECEPTOR ASSAY

MATERIALS

Human fibrinogen receptor (ant>p₃) was purified from outdated platelets. (Pytela, R., Pierschbacher, M.D., Argraves, S., Suzuki, S., and Rouslahti, E. Arginine-Glycine-Aspartic acid adhesion receptors, Methods in Enzvmoloqy 144(1987):475-489.) Human vitronectin was purified from fresh frozen plasma as described in Yatohgo, T., Izumi, M., Kashiwagi, H., and Hayashi, M., Novel purification of vitronectin from human plasma by heparin affinity chromatography, Cell Structure and

Function 13(1988):281-292. Biotinylated human vitronectin was prepared by coupling NHS-biotin from Pierce Chemical Company (Rockford, IL) to purified vitronectin as previously described. (Charo, I.F., Nannizzi, L., ~ Phillips, D.R., Hsu, M.A., Scaround bottomorough, R.M., Inhibition of fibrinogen binding to GP llb/llla by a GP Ilia peptide, J. Biol. Chem,

266(3)(1991): 1415-1421.) Assay buffer, OPD substrate tablets, and RIA grade BSA were obtained from Sigma (St. Louis, MO). Anti-biotin antibody > was obtained from Calbiochem (La Jolla, CA). Linbro microtiter plates ( were obtained from Flow Labs (McLean, VA). ADP reagent was obtained from Sigma (St. Louis, MO).

«

METHODS

Solid Phase Receptor Assays

This assay is essentially the same reported in Niiya, K., Hodson, E.,

Bader, R„ Byers-Ward, V. Koziol, J.A., Plow, E.F. and Ruggeri, Z.M., Increased surface expression of the membrane glycoprotein llb/llla complex induced by platelet activation: Relationships to the binding of fibrinogen and platelet aggregation, Blood 70(1987):475-483. The purified human fibrinogen receptor (a,,4½) was diluted from stock solutions to 1.0 pg/mL in Tris-buffered saline containing 1.0 mM Ca⁺⁺, Mg⁺⁺, and

BNSDOCID: <AP

1244A I >

APO 0 12 4 4

Mn⁺⁺, pH 7.4 (TBS⁺⁺⁺). The diluted receptor was immediately transferred to Linbro microtiter plates at 100 pL/well (100 ng receptor/well). The plates were sealed and incubated overnight at 4°C to allow the receptor to bind to the wells. All remaining steps were at room temperature. The assay plates were emptied and 200 pL of 1 % RIA grade BSA in TBS⁺⁺⁺ (TBS⁺⁺⁺/BSA) were added to block exposed plastic surfaces. Following a 2 hour incubation, the assay plates were washed with TBS⁺⁺⁺ using a 96 well plate washer. Logarithmic serial dilution of the test compound and controls were made starting at a stock concentration of 2 mM and using 2 io nM biotinylated vitronectin in TBS^^/BSA as the diluent. This premixing of labeled ligand with test (or control) ligand, and subsequent transfer of 50 pL aliquots to the assay plate was carried out with a CETUS Propette robot; the final concentration of the labeled ligand was 1 nM and the highest concentration of test compound was 1.0 x 10⁴ M. The . competition occurred for two hours after which all wells were washed with

I a plate washer as before. Affinity purified horseradish peroxidase labeled goat anti-biotin antibody was diluted 1:3000 in TBS⁺⁺⁺/BSA and 125 pL were added to each well. After 30 minutes, the plates were washed and incubated with ODD/H2O2 substrate in 100 mM/L citrate buffer, pH 5.0.

The plate was read with a microtiter plate reader at a wavelength of 450 nm and when the maximum-binding control wells reached an absoround bottomance of about 1.0, the final A450 were recorded for analysis. The data were analyzed using a macro written for use with the EXCEL™ spreadsheet program. The mean, standard deviation, and %CV were determined for duplicate concentrations. The mean A^ values were normalized to the mean of four maximum-binding controls (no competitor added)(B-MAX). The normalized values were subjected to a four parameter curve fit algorithm, [Robard et al., Int. Atomic Energy Agency, Vienna, pp 469 (1977)], plotted on a semi-log scale, and the computed concentration corresponding to inhibition of 50% of the maximum binding of biotinylated vitronectin (IC50) and corresponding R² was reported for £6810/00 /2/dV

BNSDOCID: <AP__1244A_L>

those compounds exhibiting greater than 50% inhibition at the highest concentration tested; otherwise the 1C» is reported as being greater than the highest concentration tested. 3-[[2-[I5-[(aminoiminomethyl)amino)-1oxopentylJamino]-1-oxoethyl]amino]-3-pyridinepropanoic acid [USSN

08/375,338, Example 1] which is a potent α_νβ₃ antagonist (IC»)in the range 3-10 nM) was included on each plate as a positive control.

Human Platelet Rich Plasma Assays

Healthy aspirin free donors were selected from a pool of volunteers.

io The harvesting of platelet rich plasma and subsequent ADP induced platelet aggregation assays were performed as described in Zucker, M.B., Platelet Aggregation Measured by the Photometric Method, Methods in

... Enzymoloov 169(1989):117-133. Standard venipuncture techniques using

X. a butterfly allowed the withdrawal of 45 mL of whole blood into a 60 mL syringe containing 5 mL of 3.8% trisodium citrate. Following thorough mixing in the syringe, the anti-coagulated whole blood was transferred to a 50 mL conical polyethylene tube. The blood was centrifuged at room temperature for 12 minutes at 200 mg to sediment non-platelet cells.

L* Platelet rich plasma was removed to a polyethylene tube and stored at room temperature until used. Platelet poor plasma was obtained from a second centrifugation of the remaining blood at 2000 xg for 15 minutes.

' Platelet counts are typically 300,000 to 500,000 per microliter. Platelet rich plasma (0.45 mL) was aliquoted into siliconized cuvettes and stirred (1100 rpm) at 37’C for 1 minute prior to adding 50 uL of pre-diluted test compound. After 1 minute of mixing, aggregation was initiated by the addition of 50 uL of 200 uM ADP. Aggregation was recorded for 3 minutes in a Payton dual channel aggregometer (Payton Scientific, Buffalo, NY). The percent inhibition of maximal response (saline control) for a series of test compound dilutions was used to determine a dose response curve. All compounds were tested in duplicate and the concentration of half-maximal inhibition (IC») was calculated graphically from the dose response curve for those compounds which exhibited 50% or greater inhibition at the ι oo

BNSDOCID: <AP

1244A I >

APO 0 12*4 highest concentration tested; otherwise, the IC» is reported as being greater than the highest concentration tested.

Example	ανβ3 ICso (nM)	nb/Hla 1C» (nM)
1	0.88	310
2	1,04	430
3	23.7	2440
4	2.02	575
5	2.13	744
6	6.46	919
7	1.01	262
8	0.40	131
9	0.37	388
9 ·Ηα	0.82	226.2
10	2.26	641
11	-	-
12	9.59	1060

AP/P/ 0 0 / 0 1 8 9 3 ! Ol

BNSDOCID: <AP_1244A_I_>

APG 0 1 2 A A

Claims (15)

1. AMENDED CLAIMS

   1. A compound of the formula

   Η Η π H wherein X and Y are the same or different halo group; R is H or ^ci“^ciO“alkyl; and pharmaceutically acceptable salts thereof.
2. 2. A compound according to Claim 1 selected from the group consisting of;

   ΑΡ/Γ/ 0 0*01893

   BNSDOCID: <AP_1244A_I_>

   %

   -103ΑΡϋ 0 12 4 4

   ΑΡ/Γ/ 0 0 /0 18 9 3

   BNSDOCID: <ΑΡ_1244Α_Ι_>

   APO 0 12 4 4

   ΑΡ/Γ/ 0 0 / 0 1 β 9 3

   BNSDOCID: <AP_ 1244A_|_>

   -105- APOO 1 24 « wherein R is H or alkyl; or pharmaceutically acceptable salts thereof.

   i
3. 3. A compound according to Claim 1 selected from the group consisting of

   AP/P/ 0 0 / 0 1 8 9 3

   BNSDOCID: <AP_1244A_I_>

   APO 0 12 4 4

   APT/ 0 0/01893

   BNSDOCID: <AP.

   1244A I >

   APOΟ 12 4 4

   -107- ...

   AP/P/ 0 0*01855

   BNSDOCID: <AP_1244A_I_>

   _1θΒ. AMVUI244 £6810/00 /U/dV

   BNSDOCID: <ΑΡ_1244A L>

   AP Ο Ο 1 2 4 4

   109

   ')
4. 4. A pharmaceutical composition comprising a therapeutically effective amount of a compound according to claim 1 and a pharmaceutically acceptable carrier.
5. 5. A pharmaceutical composition comprising a therapeutically effective amount of a compound according to claim 2 and a pharmaceutically acceptable carrier.
6. 6. A pharmaceutical composition comprising a therapeutically effective amount of a compound according to claim 3 and a pharmaceutically acceptable carrier.
7. 7. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating conditions medicated by the α_νβ₃ integrin in a mammal in need of such treatment.
8. 8. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating tumor metastasis.
9. 9. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating solid tumor growth.
10. 10. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating angiogenesis.
11. 11. The use of the compound of claim.1, 2 or 3 for preparing a medicament for treating osteoporosis.
12. 12. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating humoral hypercalcemia of malignancy.
13. 13. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating smooth muscle cell migration.
14. 14. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating rheumatoid arthritis.
15. 15. The use of the compound of claim 1, 2 or 3 for preparing a medicament for treating macular degeneration.