NO20033672L - Anvendelse av fusjonsproteiner der den N-terminale delen er et hirudinderivat for fremstilling av rekombinante proteiner via sekresjon fragj¶r - Google Patents

Anvendelse av fusjonsproteiner der den N-terminale delen er et hirudinderivat for fremstilling av rekombinante proteiner via sekresjon fragj¶r

Info

Publication number: NO20033672L
Authority: NO; Norway
Prior art keywords: nucleic acid; acid sequence; hirudin; production; protein
Prior art date: 2001-02-20

Application number

NO20033672A

Other languages

English (en)

Other versions

NO331376B1 (no

NO20033672D0 (no

Inventor

Paul Habermann

Original Assignee

Aventis Pharma Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-02-20

Filing date

2003-08-19

Publication date

2003-10-17

2003-08-19 Application filed by Aventis Pharma Gmbh filed Critical Aventis Pharma Gmbh

2003-08-19 Publication of NO20033672D0 publication Critical patent/NO20033672D0/no

2003-10-17 Publication of NO20033672L publication Critical patent/NO20033672L/no

2011-12-12 Publication of NO331376B1 publication Critical patent/NO331376B1/no

Links

WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical class C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 title abstract 4
102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
102000037865 fusion proteins Human genes 0.000 title 1
108020001507 fusion proteins Proteins 0.000 title 1
230000028327 secretion Effects 0.000 title 1
150000007523 nucleic acids Chemical group 0.000 abstract 5
108020004705 Codon Proteins 0.000 abstract 4
108091028043 Nucleic acid sequence Proteins 0.000 abstract 4
108090000623 proteins and genes Proteins 0.000 abstract 3
102000004169 proteins and genes Human genes 0.000 abstract 3
239000000126 substance Substances 0.000 abstract 3
239000004475 Arginine Substances 0.000 abstract 2
102000007625 Hirudins Human genes 0.000 abstract 2
108010007267 Hirudins Proteins 0.000 abstract 2
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 2
229940006607 hirudin Drugs 0.000 abstract 2
239000004472 Lysine Substances 0.000 abstract 1
KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
102000001708 Protein Isoforms Human genes 0.000 abstract 1
108010029485 Protein Isoforms Proteins 0.000 abstract 1
108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
239000013612 plasmid Substances 0.000 abstract 1
239000013598 vector Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Zoology (AREA)
Biomedical Technology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Mycology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Diabetes (AREA)
Endocrinology (AREA)
Toxicology (AREA)
Tropical Medicine & Parasitology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

NO20033672A 2001-02-20 2003-08-19 DNA-molekyl kodende for fusjonsproteiner der den N-terminale delen er et hirudinderivat for fremstilling av rekombinante proteiner via sekresjon fra gjaer, samt multikopivektor, plasmid, vertscelle og fremgangsmater for fermentering og fremstilling NO331376B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE10108211A DE10108211A1 (de)	2001-02-20	2001-02-20	Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
PCT/EP2002/001308 WO2002070722A1 (en)	2001-02-20	2002-02-08	Use of fusion proteins whose n-terminal part is a hirudin derivative for the production of recombinant proteins via secretion by yeasts

Publications (3)

Publication Number	Publication Date
NO20033672D0 NO20033672D0 (no)	2003-08-19
NO20033672L true NO20033672L (no)	2003-10-17
NO331376B1 NO331376B1 (no)	2011-12-12

Family

ID=7674912

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO20033672A NO331376B1 (no)	2001-02-20	2003-08-19	DNA-molekyl kodende for fusjonsproteiner der den N-terminale delen er et hirudinderivat for fremstilling av rekombinante proteiner via sekresjon fra gjaer, samt multikopivektor, plasmid, vertscelle og fremgangsmater for fermentering og fremstilling

Country Status (21)

Country	Link
US (1)	US20030176673A1 (no)
EP (1)	EP1364032B1 (no)
JP (1)	JP4199543B2 (no)
KR (1)	KR100858833B1 (no)
CN (1)	CN1279171C (no)
AT (1)	ATE342367T1 (no)
AU (1)	AU2002250903B2 (no)
BR (1)	BR0207379A (no)
CA (1)	CA2439042C (no)
CY (1)	CY1106274T1 (no)
DE (2)	DE10108211A1 (no)
DK (1)	DK1364032T3 (no)
ES (1)	ES2272696T3 (no)
IL (2)	IL157416A0 (no)
MX (1)	MXPA03007116A (no)
NO (1)	NO331376B1 (no)
NZ (1)	NZ527642A (no)
PE (1)	PE20020857A1 (no)
PT (1)	PT1364032E (no)
WO (1)	WO2002070722A1 (no)
ZA (1)	ZA200305868B (no)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7638618B2 (en) *	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
DE102006031962A1 (de)	2006-07-11	2008-01-17	Sanofi-Aventis Deutschland Gmbh	Amidiertes Insulin Glargin
DE102006031955A1 (de)	2006-07-11	2008-01-17	Sanofi-Aventis Deutschland Gmbh	Verfahren zur Herstellung von Insulinanaloga mit dibasischem B-Kettenende
DE102008003566A1 (de)	2008-01-09	2009-07-16	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
DE102008025007A1 (de)	2008-05-24	2009-11-26	Sanofi-Aventis Deutschland Gmbh	Neue Insulinderivate mit extrem verzögertem Zeit-/ Wirkungsprofil
DK2229407T3 (en)	2008-01-09	2017-02-27	Sanofi Aventis Deutschland	NEW INSULIN DERIVATIVES WITH EXTREMELY DELAYED TIME / EFFECTS PROFILE
CA2711752A1 (en)	2008-01-09	2009-07-16	Sanofi-Aventis Deutschland Gmbh	Novel insulin derivatives having an extremely delayed time-action profile
CN103736082A (zh)	2008-10-17	2014-04-23	赛诺菲-安万特德国有限公司	胰岛素和glp-1激动剂的组合
AU2010317995B2 (en)	2009-11-13	2014-04-17	Sanofi-Aventis Deutschland Gmbh	Pharmaceutical composition comprising a GLP-1 agonist, an insulin, and methionine
CN102711804B (zh)	2009-11-13	2015-09-16	赛诺菲-安万特德国有限公司	包含glp-1激动剂和甲硫氨酸的药物组合物
PT2611458T (pt)	2010-08-30	2016-12-16	Sanofi Aventis Deutschland	Utilização de ave0010 para o fabrico de um medicamento para o tratamento da diabetes mellitus tipo 2
US9821032B2 (en)	2011-05-13	2017-11-21	Sanofi-Aventis Deutschland Gmbh	Pharmaceutical combination for improving glycemic control as add-on therapy to basal insulin
ES2550357T3 (es)	2011-08-29	2015-11-06	Sanofi-Aventis Deutschland Gmbh	Combinación farmacéutica para su uso en el control glucémico en pacientes de diabetes de tipo 2
AR087744A1 (es)	2011-09-01	2014-04-16	Sanofi Aventis Deutschland	Composicion farmaceutica para uso en el tratamiento de una enfermedad neurodegenerativa
KR102578030B1 (ko)	2014-12-12	2023-09-14	사노피-아벤티스 도이칠란트 게엠베하	인슐린 글라진／릭시세나티드 고정비 제형
TWI748945B (zh)	2015-03-13	2021-12-11	德商賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患治療
TW201705975A (zh)	2015-03-18	2017-02-16	賽諾菲阿凡提斯德意志有限公司	第２型糖尿病病患之治療

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5705355A (en) *	1984-03-27	1998-01-06	Transgene, S.A.	Hirudin, pharmaceutical compositions comprising it and their use
WO1985004418A1 (fr) *	1984-03-27	1985-10-10	Transgene S.A.	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
DE3738541A1 (de) *	1987-11-13	1989-05-24	Hoechst Ag	Verfahren zur isolierung und reinigung von hirudin
US6875589B1 (en) *	1988-06-23	2005-04-05	Hoechst Aktiengesellschaft	Mini-proinsulin, its preparation and use
GB8927722D0 (en) *	1989-12-07	1990-02-07	British Bio Technology	Proteins and nucleic acids
DE4009268A1 (de) *	1990-03-22	1991-09-26	Consortium Elektrochem Ind	Sekretion von hirudinderivaten
DK0511393T3 (da) *	1990-11-08	1996-12-09	Japan Energy Corp	Hirudin-mutant, fremstilling af denne, antikoagulant, sekretorisk vektor, mikroorganisme transformeret af vektoren og fremstilling af produkt ud fra mikroorganismen
FI92601C (fi) *	1992-03-11	1994-12-12	Marja Makarow	Menetelmä hyötyproteiinien erittämiseksi hiivoista
DE19543737A1 (de) *	1995-11-24	1997-05-28	Hoechst Ag	Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de) *	1995-11-28	1997-06-05	Hoechst Ag	Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
WO1997033973A1 (en) *	1996-03-13	1997-09-18	Delta Biotechnology Limited	Fermentation control
DE10033195A1 (de) *	2000-07-07	2002-03-21	Aventis Pharma Gmbh	Bifunktionale Fusionsproteine aus Hirudin und TAP
US7202059B2 (en) *	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium

2001
- 2001-02-20 DE DE10108211A patent/DE10108211A1/de not_active Withdrawn
2002
- 2002-02-06 PE PE2002000082A patent/PE20020857A1/es not_active Application Discontinuation
- 2002-02-08 CN CNB028068491A patent/CN1279171C/zh not_active Expired - Fee Related
- 2002-02-08 EP EP02719782A patent/EP1364032B1/en not_active Expired - Lifetime
- 2002-02-08 CA CA2439042A patent/CA2439042C/en not_active Expired - Fee Related
- 2002-02-08 ES ES02719782T patent/ES2272696T3/es not_active Expired - Lifetime
- 2002-02-08 JP JP2002570745A patent/JP4199543B2/ja not_active Expired - Fee Related
- 2002-02-08 MX MXPA03007116A patent/MXPA03007116A/es active IP Right Grant
- 2002-02-08 WO PCT/EP2002/001308 patent/WO2002070722A1/en not_active Ceased
- 2002-02-08 AU AU2002250903A patent/AU2002250903B2/en not_active Ceased
- 2002-02-08 KR KR1020037010932A patent/KR100858833B1/ko not_active Expired - Fee Related
- 2002-02-08 AT AT02719782T patent/ATE342367T1/de active
- 2002-02-08 BR BR0207379-0A patent/BR0207379A/pt not_active Application Discontinuation
- 2002-02-08 DE DE60215315T patent/DE60215315T2/de not_active Expired - Lifetime
- 2002-02-08 PT PT02719782T patent/PT1364032E/pt unknown
- 2002-02-08 NZ NZ527642A patent/NZ527642A/en not_active IP Right Cessation
- 2002-02-08 DK DK02719782T patent/DK1364032T3/da active
- 2002-02-08 IL IL15741602A patent/IL157416A0/xx unknown
- 2002-02-19 US US10/076,632 patent/US20030176673A1/en not_active Abandoned
2003
- 2003-07-30 ZA ZA2003/05868A patent/ZA200305868B/en unknown
- 2003-08-14 IL IL157416A patent/IL157416A/en not_active IP Right Cessation
- 2003-08-19 NO NO20033672A patent/NO331376B1/no not_active IP Right Cessation
2006
- 2006-11-30 CY CY20061101726T patent/CY1106274T1/el unknown

Also Published As

Publication number	Publication date
DE60215315D1 (de)	2006-11-23
HK1065068A1 (en)	2005-02-08
DK1364032T3 (da)	2007-02-19
CN1526021A (zh)	2004-09-01
CY1106274T1 (el)	2011-10-12
IL157416A (en)	2009-08-03
ES2272696T3 (es)	2007-05-01
KR20030074841A (ko)	2003-09-19
IL157416A0 (en)	2004-03-28
PT1364032E (pt)	2007-01-31
AU2002250903B2 (en)	2006-12-07
NO331376B1 (no)	2011-12-12
NZ527642A (en)	2005-03-24
WO2002070722A1 (en)	2002-09-12
US20030176673A1 (en)	2003-09-18
JP4199543B2 (ja)	2008-12-17
ZA200305868B (en)	2005-02-23
BR0207379A (pt)	2004-06-15
EP1364032B1 (en)	2006-10-11
CA2439042C (en)	2013-06-18
ATE342367T1 (de)	2006-11-15
CN1279171C (zh)	2006-10-11
DE60215315T2 (de)	2007-05-24
MXPA03007116A (es)	2003-11-18
EP1364032A1 (en)	2003-11-26
PE20020857A1 (es)	2002-11-04
NO20033672D0 (no)	2003-08-19
KR100858833B1 (ko)	2008-09-17
DE10108211A1 (de)	2002-08-22
CA2439042A1 (en)	2002-09-12
JP2004518446A (ja)	2004-06-24

Publication	Publication Date	Title
NO20033672L (no)	2003-10-17	Anvendelse av fusjonsproteiner der den N-terminale delen er et hirudinderivat for fremstilling av rekombinante proteiner via sekresjon fragj¶r
Vedvick et al.	1991	High-level secretion of biologically active aprotinin from the yeast Pichia pastoris
AU2002231784B2 (en)	2006-10-26	Fusion protein for the secretion of a protein of interest into the supernatant of the bacterial culture
JP2024081707A (ja)	2024-06-18	延長組換えポリペプチドおよび延長組換えポリペプチドを含む組成物
JP5697831B2 (ja)	2015-04-08	単鎖インシュリン
Hébert et al.	1990	Endothelial and leukocyte forms of IL-8. Conversion by thrombin and interactions with neutrophils.
JP2566933B2 (ja)	1996-12-25	真核細胞融合タンパク質、その生産及び用途、並びに方法
EP0399666A1 (en)	1990-11-28	Fusion proteins containing N-terminal fragments of human serum albumin
DK168823B1 (da)	1994-06-20	Fusionsproteiner, hvor ballastandelen består af peptider af interleukin 2, fremgangsmåde til deres fremstilling, deres anvendelse, genstruktur, der koder for et sådant fusionsprotein, vektor indeholdende en sådan genstruktur, og E. coli-celle indeholdende en sådan vektor.
CN103328649A (zh)	2013-09-25	用于改善重组酶及其它蛋白的蛋白表达和分泌的新的信号序列
JPH05502025A (ja)	1993-04-15	第８：ｃ因子活性を有するタンパク質複合体およびその製法
Arlaud et al.	1983	Complete amino acid sequence of the catalytic chain of human complement subcomponent C. hivin. 1r
Pohlig et al.	1996	Purification, Characterization and Biological Evaluation of Recombinant Leech‐Derived Tryptase Inhibitor (rLDTI) Expressed at High Level in the Yeast Saccharomyces Cerevisiae
DK1364030T3 (da)	2006-07-17	Supersecernerbare peptider, processer til frembringelse herf og parallel forbedring af den secernerede form at et eller flere andre polypeptider
EP3719033A1 (en)	2020-10-07	Stabilized mhc i
AU597317B2 (en)	1990-05-31	A method to export gene products to the growth medium of gram negative bacteria
JPH05500615A (ja)	1993-02-12	ヒルジン及び新規のヒルジンの組換え製造方法
EP0330700A4 (en)	1990-01-08	Human prourokinase-like polypeptide.
US7344856B1 (en)	2008-03-18	Method of controlling cleavage by OmpT protease
Okabayashi et al.	1996	Secretory production of recombinant urokinase-type plasminogen activator-annexin V chimeras in Pichia pastoris
JPH03259084A (ja)	1991-11-19	トロンビン結合性物質の製造法
Przysiecki et al.	1992	Characterization of recombinant antistasin secreted by Saccharomyces cerevisiae
Yang et al.	2006	Expression and localization of recombinant human EDG-1 receptors in Pichia pastoris

Legal Events

Date	Code	Title	Description
2015-09-28	MM1K	Lapsed by not paying the annual fees