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MXPA03001879A - Metodo y sistema para detectar acidos nucleicos. - Google Patents

Metodo y sistema para detectar acidos nucleicos.

Info

Publication number
MXPA03001879A
MXPA03001879A MXPA03001879A MXPA03001879A MXPA03001879A MX PA03001879 A MXPA03001879 A MX PA03001879A MX PA03001879 A MXPA03001879 A MX PA03001879A MX PA03001879 A MXPA03001879 A MX PA03001879A MX PA03001879 A MXPA03001879 A MX PA03001879A
Authority
MX
Mexico
Prior art keywords
nucleic acid
target nucleic
nucleic acids
sample
detecting
Prior art date
Application number
MXPA03001879A
Other languages
English (en)
Inventor
Fernando Patolsky
Original Assignee
Yissum Res Dev Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co filed Critical Yissum Res Dev Co
Publication of MXPA03001879A publication Critical patent/MXPA03001879A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B01J2219/00529—DNA chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/0061—The surface being organic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00713—Electrochemical synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Un metodo para la deteccion de un acido nucleico blanco en una muestra que contiene una mezcla de acidos nucleicos. El metodo comprende: (a) proveer una superficie solida; (b) unir a la superficie solida una sonda de oligonucleotido complementaria con un segmento del acido nucleico blanco; (c) poner en contacto la superficie con la muestra y permitir asi que la sonda se una al acido nucleico blanco; (d) incubar el acido nucleico blanco unido con 4 tipos de nucleotido y un biocatalizador de replicacion y formar asi un ensamble de acido nucleico de hebras multiples, en donde por lo menos uno de los tipos de nucleotido es unido mediante un marcador; y (e) detectar el marcador en el ensamble de acido nucleico de hebras multiples y detectar asi el acido nucleico blanco. Asimismo, se describen un sistema para identificar una secuencia de acido nucleico blanco en una muestra y un equipo para usarse en el metodo.
MXPA03001879A 2000-09-04 2001-08-29 Metodo y sistema para detectar acidos nucleicos. MXPA03001879A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL13822900A IL138229A (en) 2000-09-04 2000-09-04 Method and system for detecting nucleic acids
PCT/IL2001/000808 WO2002020838A2 (en) 2000-09-04 2001-08-29 Method and system for detecting nucleic acids

Publications (1)

Publication Number Publication Date
MXPA03001879A true MXPA03001879A (es) 2004-04-20

Family

ID=11074586

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA03001879A MXPA03001879A (es) 2000-09-04 2001-08-29 Metodo y sistema para detectar acidos nucleicos.

Country Status (9)

Country Link
US (1) US20040023258A1 (es)
EP (1) EP1379684A2 (es)
JP (1) JP2004526409A (es)
AU (1) AU2001282491A1 (es)
CA (1) CA2421204A1 (es)
IL (1) IL138229A (es)
MX (1) MXPA03001879A (es)
NZ (1) NZ524956A (es)
WO (1) WO2002020838A2 (es)

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DE10311315A1 (de) * 2003-03-14 2004-09-30 Apibio Sas Verfahren und Vorrichtung zur Detektion von Biomolekülen
CA2524263A1 (en) * 2003-05-02 2004-11-18 Geneohm Sciences, Inc. Electrochemical method to measure dna attachment to an electrode surface in the presence of molecular oxygen
US7642064B2 (en) 2003-06-24 2010-01-05 Ventana Medical Systems, Inc. Enzyme-catalyzed metal deposition for the enhanced detection of analytes of interest
AU2004290036B2 (en) * 2003-11-10 2011-08-04 Geneohm Sciences, Inc. Nucleic acid detection method having increased sensitivity
JP2006182757A (ja) * 2004-10-06 2006-07-13 Japan Science & Technology Agency π共役型電気化学活性非天然ヌクレオシドを用いる相補鎖核酸分子配列検出方法及びＳＮＰ検出方法
DE102005037436A1 (de) 2005-08-04 2007-02-15 Siemens Ag Verfahren und System zur Konzentrationsbestimmung eines Analyt-Enzym-Komplexes oder Analyt-Enzym-Konjugats, insbesondere zur elektrochemischen Detektion des Analyten, und zugehörige Messvorrichtung
US20070231794A1 (en) * 2005-09-21 2007-10-04 Combimatrix Corporation Process to detect binding events on an electrode microarray using enzymes
US8855955B2 (en) * 2005-09-29 2014-10-07 Custom Array, Inc. Process and apparatus for measuring binding events on a microarray of electrodes
US7820391B2 (en) 2007-11-06 2010-10-26 Osmetech Molecular Diagnostics Baseless nucleotide analogues and uses thereof
CN101241125B (zh) * 2008-03-10 2013-02-13 施晓燕 一种用于液相测量的压电传感器及封装方法
US20100069253A1 (en) * 2008-09-12 2010-03-18 Gindilis Andrei L Impedance Spectroscopy Measurement of DNA
WO2011102065A1 (ja) * 2010-02-17 2011-08-25 株式会社 村田製作所 質量検出センサーを用いた検出方法
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EP3121288A1 (en) 2012-07-27 2017-01-25 Ohmx Corporation Electronic measurements of monolayers following homogeneous reactions of their components
CA2880104A1 (en) * 2012-07-27 2014-01-30 Ohmx Corporation Electric measurement of monolayers following pro-cleave detection of presence and activity of enzymes and other target analytes
US20150045254A1 (en) * 2013-08-07 2015-02-12 Xagenic Inc. Systems, methods and devices for electrochemical detection using helper oligonucleotides
HUE054415T2 (hu) 2013-12-12 2021-09-28 Hollister Inc Toalettben leöblíthetõ katéterek
LT3079750T (lt) 2013-12-12 2020-07-27 Hollister Incorporated Srove nuplaunami kateteriai
EP3079752B1 (en) 2013-12-12 2020-04-01 Hollister Incorporated Flushable catheters
EP3079748B1 (en) 2013-12-12 2020-05-27 Hollister Incorporated Flushable disintegration catheter
CN105866226A (zh) * 2016-06-07 2016-08-17 辽宁科技大学 一种葡萄糖氧化酶生物传感器的制备及使用方法
JP6346242B2 (ja) * 2016-11-04 2018-06-20 株式会社東芝 核酸検出方法及びアッセイキット
CN112326763B (zh) * 2020-09-24 2022-10-21 福建医科大学附属第一医院 一种可用于临床样品等位基因分型的dna纳米筛可再生电化学传感器

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Also Published As

Publication number Publication date
NZ524956A (en) 2005-01-28
WO2002020838A2 (en) 2002-03-14
EP1379684A2 (en) 2004-01-14
US20040023258A1 (en) 2004-02-05
CA2421204A1 (en) 2002-03-14
AU2001282491A1 (en) 2002-03-22
IL138229A (en) 2005-03-20
JP2004526409A (ja) 2004-09-02
WO2002020838A3 (en) 2003-10-23
IL138229A0 (en) 2001-10-31

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