AU2001282920A1 - Methods for detecting and assaying nucleic acid sequences - Google Patents
Methods for detecting and assaying nucleic acid sequencesInfo
- Publication number
- AU2001282920A1 AU2001282920A1 AU2001282920A AU8292001A AU2001282920A1 AU 2001282920 A1 AU2001282920 A1 AU 2001282920A1 AU 2001282920 A AU2001282920 A AU 2001282920A AU 8292001 A AU8292001 A AU 8292001A AU 2001282920 A1 AU2001282920 A1 AU 2001282920A1
- Authority
- AU
- Australia
- Prior art keywords
- detecting
- methods
- nucleic acid
- acid sequences
- assaying nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical group 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000002493 microarray Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention is directed to a method for determining the presence of a specific nucleotide sequence in an RNA reagent of a target sample utilizing a capture reagent having at least one first arm containing a label capable of emitting a detectable signal and at least one second arm having a nucleotide sequence complementary to a capture sequence attached to the RNA reagent on a microarray.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21939700P | 2000-07-19 | 2000-07-19 | |
| US60219397 | 2000-07-19 | ||
| PCT/US2001/022818 WO2002006511A2 (en) | 2000-07-19 | 2001-07-19 | Methods for detecting and assaying nucleic acid sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001282920A1 true AU2001282920A1 (en) | 2002-01-30 |
Family
ID=22819100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001282920A Abandoned AU2001282920A1 (en) | 2000-07-19 | 2001-07-19 | Methods for detecting and assaying nucleic acid sequences |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20020072060A1 (en) |
| EP (1) | EP1319179B1 (en) |
| JP (1) | JP2004506179A (en) |
| AT (1) | ATE388402T1 (en) |
| AU (1) | AU2001282920A1 (en) |
| DE (1) | DE60133111T2 (en) |
| WO (1) | WO2002006511A2 (en) |
Families Citing this family (37)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5138851B2 (en) | 1999-06-28 | 2013-02-06 | オクラホマ メディカル リサーチ ファウンデーション | Catalytically active recombinant memapsin and methods of use thereof |
| US20020051981A1 (en) * | 2000-03-08 | 2002-05-02 | Robert Getts | Methods for assay and detection on a microarray |
| US6692700B2 (en) | 2001-02-14 | 2004-02-17 | Handylab, Inc. | Heat-reduction methods and systems related to microfluidic devices |
| US7010391B2 (en) | 2001-03-28 | 2006-03-07 | Handylab, Inc. | Methods and systems for control of microfluidic devices |
| US7829025B2 (en) | 2001-03-28 | 2010-11-09 | Venture Lending & Leasing Iv, Inc. | Systems and methods for thermal actuation of microfluidic devices |
| US8895311B1 (en) | 2001-03-28 | 2014-11-25 | Handylab, Inc. | Methods and systems for control of general purpose microfluidic devices |
| US20040265883A1 (en) * | 2003-06-27 | 2004-12-30 | Biocept, Inc. | mRNA expression analysis |
| US20050084880A1 (en) * | 2003-07-11 | 2005-04-21 | Ronald Duman | Systems and methods for diagnosing & treating psychological and behavioral conditions |
| EP2402089A1 (en) | 2003-07-31 | 2012-01-04 | Handylab, Inc. | Processing particle-containing samples |
| US8852862B2 (en) | 2004-05-03 | 2014-10-07 | Handylab, Inc. | Method for processing polynucleotide-containing samples |
| EP1769088A2 (en) * | 2004-06-30 | 2007-04-04 | Whitehead Institute For Biomedical Research | Novel methods for high-throughput genome-wide location analysis |
| CN1312293C (en) * | 2004-11-08 | 2007-04-25 | 博奥生物有限公司 | High flux biochip and application thereof |
| US20070048741A1 (en) * | 2005-08-24 | 2007-03-01 | Getts Robert C | Methods and kits for sense RNA synthesis |
| US20070105124A1 (en) * | 2005-11-08 | 2007-05-10 | Getts Robert C | Methods and kits for nucleic acid amplification |
| US11806718B2 (en) | 2006-03-24 | 2023-11-07 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
| DK2001990T3 (en) | 2006-03-24 | 2016-10-03 | Handylab Inc | Integrated microfluidic sample processing system and method for its use |
| US7998708B2 (en) | 2006-03-24 | 2011-08-16 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| US10900066B2 (en) | 2006-03-24 | 2021-01-26 | Handylab, Inc. | Microfluidic system for amplifying and detecting polynucleotides in parallel |
| US8883490B2 (en) | 2006-03-24 | 2014-11-11 | Handylab, Inc. | Fluorescence detector for microfluidic diagnostic system |
| WO2008061165A2 (en) | 2006-11-14 | 2008-05-22 | Handylab, Inc. | Microfluidic cartridge and method of making same |
| AU2008257419B2 (en) * | 2007-05-23 | 2013-10-24 | The Trustees Of The University Of Pennsylvania | Targeted carriers for intracellular drug delivery |
| US9186677B2 (en) | 2007-07-13 | 2015-11-17 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| US8105783B2 (en) | 2007-07-13 | 2012-01-31 | Handylab, Inc. | Microfluidic cartridge |
| US8182763B2 (en) | 2007-07-13 | 2012-05-22 | Handylab, Inc. | Rack for sample tubes and reagent holders |
| US8287820B2 (en) | 2007-07-13 | 2012-10-16 | Handylab, Inc. | Automated pipetting apparatus having a combined liquid pump and pipette head system |
| US9618139B2 (en) | 2007-07-13 | 2017-04-11 | Handylab, Inc. | Integrated heater and magnetic separator |
| US8133671B2 (en) | 2007-07-13 | 2012-03-13 | Handylab, Inc. | Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples |
| ES2877602T3 (en) | 2007-07-13 | 2021-11-17 | Handylab Inc | Polynucleotide Capture Materials and Procedures for Using Them |
| USD787087S1 (en) | 2008-07-14 | 2017-05-16 | Handylab, Inc. | Housing |
| EP2697657B8 (en) | 2011-04-15 | 2017-08-16 | Becton, Dickinson and Company | Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection |
| DK2761305T3 (en) | 2011-09-30 | 2017-11-20 | Becton Dickinson Co | United Reagent Strip |
| USD692162S1 (en) | 2011-09-30 | 2013-10-22 | Becton, Dickinson And Company | Single piece reagent holder |
| CN104040238B (en) | 2011-11-04 | 2017-06-27 | 汉迪拉布公司 | Polynucleotides sample preparation apparatus |
| CA2863637C (en) | 2012-02-03 | 2021-10-26 | Becton, Dickinson And Company | External files for distribution of molecular diagnostic tests and determination of compatibility between tests |
| US9651549B2 (en) * | 2012-07-13 | 2017-05-16 | Genisphere, Llc | Lateral flow assays using DNA dendrimers |
| WO2014153394A1 (en) | 2013-03-21 | 2014-09-25 | Genisphere, Llc | Cellular delivery of dna intercalating agents |
| WO2017143156A1 (en) | 2016-02-19 | 2017-08-24 | Genisphere Llc | Nucleic acid carriers and therapeutic methods of use |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE23752T1 (en) * | 1980-08-21 | 1986-12-15 | Oriel Scient Ltd | OPTICAL ANALYZER. |
| US5175270A (en) * | 1986-09-10 | 1992-12-29 | Polyprobe, Inc. | Reagents for detecting and assaying nucleic acid sequences |
| AU2253397A (en) * | 1996-01-23 | 1997-08-20 | Affymetrix, Inc. | Nucleic acid analysis techniques |
| US6586193B2 (en) * | 1996-04-25 | 2003-07-01 | Genicon Sciences Corporation | Analyte assay using particulate labels |
| US6361940B1 (en) * | 1996-09-24 | 2002-03-26 | Qiagen Genomics, Inc. | Compositions and methods for enhancing hybridization and priming specificity |
| US5853993A (en) * | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| US6060246A (en) * | 1996-11-15 | 2000-05-09 | Avi Biopharma, Inc. | Reagent and method for isolation and detection of selected nucleic acid sequences |
| DE69834645T2 (en) * | 1997-07-29 | 2007-05-03 | Polyprobe, Inc. | DENDRITIC NUCLEIC ACIDS WITH MAXIMIZED SELF-ASSOCIATION |
| US6261779B1 (en) * | 1998-11-10 | 2001-07-17 | Bio-Pixels Ltd. | Nanocrystals having polynucleotide strands and their use to form dendrimers in a signal amplification system |
| KR20020007332A (en) * | 1999-03-18 | 2002-01-26 | 추후제출 | One step sample preparation and detection of nucleic acids in complex biological samples |
| US20020094538A1 (en) * | 2001-01-13 | 2002-07-18 | Getts Robert C. | Methods for detecting and assaying nucleic acid sequences using temperature cycling |
| US20020051981A1 (en) * | 2000-03-08 | 2002-05-02 | Robert Getts | Methods for assay and detection on a microarray |
| WO2003012147A1 (en) * | 2001-02-20 | 2003-02-13 | Datascope Investment Corp. | Method for reusing standard blots and microarrays utilizing dna dendrimer technology |
| EP1438435B1 (en) * | 2001-08-31 | 2007-10-31 | Datascope Investment Corp. | Methods for blocking nonspecific hybridisations of nucleic acid sequences |
-
2001
- 2001-07-19 AT AT01961674T patent/ATE388402T1/en not_active IP Right Cessation
- 2001-07-19 WO PCT/US2001/022818 patent/WO2002006511A2/en not_active Ceased
- 2001-07-19 DE DE60133111T patent/DE60133111T2/en not_active Expired - Lifetime
- 2001-07-19 EP EP01961674A patent/EP1319179B1/en not_active Expired - Lifetime
- 2001-07-19 AU AU2001282920A patent/AU2001282920A1/en not_active Abandoned
- 2001-07-19 JP JP2002512402A patent/JP2004506179A/en not_active Withdrawn
- 2001-07-19 US US09/908,950 patent/US20020072060A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002006511A2 (en) | 2002-01-24 |
| EP1319179A2 (en) | 2003-06-18 |
| EP1319179A4 (en) | 2005-01-19 |
| ATE388402T1 (en) | 2008-03-15 |
| US20020072060A1 (en) | 2002-06-13 |
| JP2004506179A (en) | 2004-02-26 |
| DE60133111D1 (en) | 2008-04-17 |
| DE60133111T2 (en) | 2009-03-12 |
| EP1319179B1 (en) | 2008-03-05 |
| WO2002006511A3 (en) | 2002-04-11 |
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