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MX9800050A - Deteccion electroquimica de la hibridacion del acido nucleico. - Google Patents

Deteccion electroquimica de la hibridacion del acido nucleico.

Info

Publication number
MX9800050A
MX9800050A MX9800050A MX9800050A MX9800050A MX 9800050 A MX9800050 A MX 9800050A MX 9800050 A MX9800050 A MX 9800050A MX 9800050 A MX9800050 A MX 9800050A MX 9800050 A MX9800050 A MX 9800050A
Authority
MX
Mexico
Prior art keywords
nucleic acid
oxidation
reduction reaction
acid hybridization
electrochemical detection
Prior art date
Application number
MX9800050A
Other languages
English (en)
Other versions
MXPA98000050A (es
Inventor
Thorp H Holden
Dean H Johnston
Original Assignee
Univ North Carolina
Hill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/667,338 external-priority patent/US5871918A/en
Application filed by Univ North Carolina, Hill filed Critical Univ North Carolina
Publication of MX9800050A publication Critical patent/MX9800050A/es
Publication of MXPA98000050A publication Critical patent/MXPA98000050A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Battery Electrode And Active Subsutance (AREA)
  • Battery Mounting, Suspending (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Un método para detectar un ácido nucleico (v.gr., ADN, ARN) que contiene por lo menos una base preseleccionada (v.gr., adenina, guanina, 6-mercaptaguanina, B-oxo-guanina y 8-oxo-adenina) comprende: hacer reaccionar el ácido nucleico con un complejo de metal de trasicion capaz de oxidar la base preseleccionada en una reaccion de oxidacion-reduccion; Detectara la reaccion de oxidacion-reduccion y determinar la presencia o ausencia del ácido nucleico a partir de la reaccion de oxidacion-reduccion detectada en la base preseleccionada; el método se puede usar en una variedad de aplicaciones, incluyendo secuenciacion de ADN, pruebas de diagnostico y análisis cuantitativos.
MXPA/A/1998/000050A 1995-06-27 1998-01-07 Deteccion electroquimica de la hibridacion del acido nucleico MXPA98000050A (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US49581795A 1995-06-27 1995-06-27
US495817 1995-06-27
US1626596P 1996-04-19 1996-04-19
US016265 1996-04-19
US08667338 1996-06-20
US08/667,338 US5871918A (en) 1996-06-20 1996-06-20 Electrochemical detection of nucleic acid hybridization
PCT/US1996/010702 WO1997001646A2 (en) 1995-06-27 1996-06-24 Electrochemical detection of nucleic acid hybridization

Publications (2)

Publication Number Publication Date
MX9800050A true MX9800050A (es) 1998-08-30
MXPA98000050A MXPA98000050A (es) 1998-11-12

Family

ID=

Also Published As

Publication number Publication date
NO976057L (no) 1998-02-24
NO976057D0 (no) 1997-12-23
CA2225935A1 (en) 1997-01-16
DK0871773T3 (da) 2002-12-09
HK1047453A1 (en) 2003-09-16
DE69623494T2 (de) 2003-04-17
ATE223498T1 (de) 2002-09-15
WO1997001646A3 (en) 1997-02-27
DE69623494D1 (de) 2002-10-10
WO1997001646A2 (en) 1997-01-16
JP2000501601A (ja) 2000-02-15
AU6337696A (en) 1997-01-30
CN1192249A (zh) 1998-09-02
AU724600B2 (en) 2000-09-28
EP1193315A1 (en) 2002-04-03
NZ311955A (en) 1999-04-29
NO20045309L (no) 1998-02-24
EP0871773A2 (en) 1998-10-21
EP0871773B1 (en) 2002-09-04

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