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MX2024005281A - Celula de levadura recombinante. - Google Patents

Celula de levadura recombinante.

Info

Publication number
MX2024005281A
MX2024005281A MX2024005281A MX2024005281A MX2024005281A MX 2024005281 A MX2024005281 A MX 2024005281A MX 2024005281 A MX2024005281 A MX 2024005281A MX 2024005281 A MX2024005281 A MX 2024005281A MX 2024005281 A MX2024005281 A MX 2024005281A
Authority
MX
Mexico
Prior art keywords
yeast cell
recombinant yeast
amino acid
acid sequence
sequence
Prior art date
Application number
MX2024005281A
Other languages
English (en)
Inventor
Mickel Leonardus August Jansen
Rij Evert Tjeerd Van
Bruijn Hans Marinus Charles Johannes De
Der Weert Marco Richard Van
Wouter Kroes
Leeuwen Johannes Gustaaf Ernst Van
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2024005281A publication Critical patent/MX2024005281A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • C12N1/165Yeast isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0008Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1217Phosphotransferases with a carboxyl group as acceptor (2.7.2)
    • CCHEMISTRY; METALLURGY
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006Glycerol dehydrogenase (1.1.1.6)
    • CCHEMISTRY; METALLURGY
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    • C12YENZYMES
    • C12Y102/00Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
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    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01008Phosphate acetyltransferase (2.3.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01019Phosphoribulokinase (2.7.1.19)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01028Triokinase (2.7.1.28)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01029Glycerone kinase (2.7.1.29), i.e. dihydroxyacetone kinase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/02Phosphotransferases with a carboxy group as acceptor (2.7.2)
    • C12Y207/02012Acetate kinase (diphosphate) (2.7.2.12)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102Alpha-glucosidase (3.2.1.20)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/01Carboxy-lyases (4.1.1)
    • C12Y401/01039Ribulose-bisphosphate carboxylase (4.1.1.39)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/02Aldehyde-lyases (4.1.2)
    • C12Y401/02009Phosphoketolase (4.1.2.9)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/02Aldehyde-lyases (4.1.2)
    • C12Y401/02022Fructose-6-phosphate phosphoketolase (4.1.2.22)
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/01Hydro-lyases (4.2.1)
    • C12Y402/01009Dihydroxy-acid dehydratase (4.2.1.9), i.e. acetohydroxyacid dehydratase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi
    • C12R2001/85Saccharomyces
    • C12R2001/865Saccharomyces cerevisiae
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Una célula de levadura recombinante que comprende una secuencia de nucleótidos que codifica una proteína, proteína que comprende una secuencia de aminoácidos de SEC ID NO: 01 o una secuencia de aminoácidos que tiene al menos un 90 % de identidad de secuencia, preferentemente al menos un 95 %, 98 % o 99 % de identidad de secuencia con la secuencia de aminoácidos de SEC ID NO: 01.
MX2024005281A 2021-11-04 2022-11-04 Celula de levadura recombinante. MX2024005281A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP21206518 2021-11-04
PCT/EP2022/080764 WO2023079050A1 (en) 2021-11-04 2022-11-04 Recombinant yeast cell

Publications (1)

Publication Number Publication Date
MX2024005281A true MX2024005281A (es) 2024-05-17

Family

ID=78709211

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2024005281A MX2024005281A (es) 2021-11-04 2022-11-04 Celula de levadura recombinante.

Country Status (5)

Country Link
US (1) US20250163361A1 (es)
EP (1) EP4426824A1 (es)
CN (1) CN118176296A (es)
MX (1) MX2024005281A (es)
WO (1) WO2023079050A1 (es)

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WO1990014423A1 (en) 1989-05-18 1990-11-29 The Infergene Company Microorganism transformation
CA2063592C (en) 1989-07-07 2003-01-21 Marco L. F. Giuseppin Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
ATE238425T1 (de) 1993-07-23 2003-05-15 Dsm Nv Selektionmarker-genfreie rekombinante stämme: verfahren zur ihrer herstellung und die verwendung dieser stämme
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
CN1169961C (zh) 1997-04-11 2004-10-06 Dsm公司 基因转变作为工具用于构建重组的工业化丝状真菌
EP1953230A1 (en) 1998-05-19 2008-08-06 DSM IP Assets B.V. Improved in vivo production of cephalosporins
WO2000037671A2 (en) 1998-12-22 2000-06-29 Dsm N.V. Improved in vivo production of cephalosporins
ES2621921T3 (es) 2004-12-22 2017-07-05 Novozymes A/S Enzimas para tratamiento de almidón
MX2009003501A (es) 2006-10-02 2009-04-16 Dsm Ip Assets Bv Ingenieria metabolica de celulas eucariotas que fermentan arabinosa.
US20110104736A1 (en) 2008-03-13 2011-05-05 Jacobus Thomas Pronk Selection of organisms capable of fermenting mixed substrates
EP2277989A1 (en) 2009-07-24 2011-01-26 Technische Universiteit Delft Fermentative glycerol-free ethanol production
WO2011066576A1 (en) * 2009-11-30 2011-06-03 Novozymes A/S Polypeptides having glucoamylase activity and polynucleotides encoding same
WO2013081456A2 (en) 2011-11-30 2013-06-06 Dsm Ip Assets B.V. Yeast strains engineered to produce ethanol from acetic acid and glycerol
CN104204205B (zh) 2012-03-27 2017-06-27 帝斯曼知识产权资产管理有限公司 克隆方法
ES2935920T3 (es) 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
CA2891455C (en) 2012-11-20 2022-07-05 Lallemand Hungary Liquidity Management Llc An electron consuming ethanol production pathway to displace glycerol formation in s. cerevisiae
PL2958986T3 (pl) 2013-02-22 2023-01-02 Dsm Ip Assets B.V. Rekombinowany mikroorganizm do zastosowania w sposobie o zwiększonej wydajności produktu
AR097480A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células de levadura convertidoras de glicerol y ácido acético con una conversión de ácido acético mejorada
AR097479A1 (es) 2013-08-29 2016-03-16 Dsm Ip Assets Bv Células convertidoras de glicerol y ácido acético con un transporte de glicerol mejorado
DK3122876T3 (da) 2014-03-28 2021-02-08 Danisco Us Inc Ændret værtscellebane for forbedret ethanolproduktion
CN106795505A (zh) 2014-10-23 2017-05-31 诺维信公司 葡糖淀粉酶变体和编码它们的多核苷酸
EP3242949B1 (en) 2015-01-06 2021-11-03 DSM IP Assets B.V. A crispr-cas system for a yeast host cell
WO2017216136A1 (en) 2016-06-14 2017-12-21 Dsm Ip Assets B.V. Recombinant yeast cell
WO2018098381A1 (en) * 2016-11-23 2018-05-31 Novozymes A/S Improved yeast for ethanol production
CN110088275A (zh) 2016-12-23 2019-08-02 帝斯曼知识产权资产管理有限公司 改进的无甘油的乙醇生产
US20200024619A1 (en) 2017-03-21 2020-01-23 Dsm Ip Assets B.V. Improved glycerol free ethanol production
CN111032854A (zh) 2017-06-13 2020-04-17 帝斯曼知识产权资产管理有限公司 重组酵母细胞
US20200283805A1 (en) 2017-09-26 2020-09-10 Dsm Ip Assets B.V. Improved process for ethanol production
US11384368B2 (en) * 2017-09-29 2022-07-12 Dsm Ip Assets B.V. Glycerol free ethanol production
WO2020043497A1 (en) 2018-08-28 2020-03-05 Dsm Ip Assets B.V. Process for the production of ethanol

Also Published As

Publication number Publication date
US20250163361A1 (en) 2025-05-22
EP4426824A1 (en) 2024-09-11
WO2023079050A1 (en) 2023-05-11
CN118176296A (zh) 2024-06-11

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