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MX2023010495A - Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos. - Google Patents

Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.

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Publication number
MX2023010495A
MX2023010495A MX2023010495A MX2023010495A MX2023010495A MX 2023010495 A MX2023010495 A MX 2023010495A MX 2023010495 A MX2023010495 A MX 2023010495A MX 2023010495 A MX2023010495 A MX 2023010495A MX 2023010495 A MX2023010495 A MX 2023010495A
Authority
MX
Mexico
Prior art keywords
cdna
complementary dna
improved methods
library preparation
methods
Prior art date
Application number
MX2023010495A
Other languages
English (en)
Inventor
Stephen M Gross
Frederick W Hyde
Robert Scott Kuersten
Allison Yunghans
Angelica Marie Barr Schalembier
Kayla Busby
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2023010495A publication Critical patent/MX2023010495A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/107—RNA dependent DNA polymerase,(i.e. reverse transcriptase)
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/327—RNAse, e.g. RNAseH
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/507—Recombinase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/137—Reactions characterised by the reaction format or use of a specific feature the purpose or use of a displacement step
    • C12Q2537/1376—Displacement by an enzyme
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
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  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

En la presente descripción se describen composiciones y métodos para preparar ADN complementario bicatenario (ADNc) de ARN. En algunas modalidades, estos métodos permiten la preparación isotérmica de ADNc. En algunas modalidades, estos métodos permiten la preparación de ADNc mesofílica o termoestable. En la presente descripción también se describen composiciones y métodos para preparar ADNc y una genoteca de fragmentos de ADNc bicatenario en un único recipiente de reacción.
MX2023010495A 2021-03-30 2022-03-29 Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos. MX2023010495A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163167909P 2021-03-30 2021-03-30
US202163234114P 2021-08-17 2021-08-17
PCT/US2022/022288 WO2022212330A1 (en) 2021-03-30 2022-03-29 Improved methods of isothermal complementary dna and library preparation

Publications (1)

Publication Number Publication Date
MX2023010495A true MX2023010495A (es) 2023-09-18

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MX2023010495A MX2023010495A (es) 2021-03-30 2022-03-29 Metodos mejorados de preparacion de genoteca y adn complementarios isotermicos.

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US (1) US20240150753A1 (es)
EP (1) EP4314282A1 (es)
JP (1) JP2024512917A (es)
KR (1) KR20230161955A (es)
AU (1) AU2022246579A1 (es)
BR (1) BR112023019959A2 (es)
CA (1) CA3214584A1 (es)
IL (1) IL307172A (es)
MX (1) MX2023010495A (es)
WO (1) WO2022212330A1 (es)

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* Cited by examiner, † Cited by third party
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CN116656787A (zh) * 2023-04-04 2023-08-29 墨卓生物科技(浙江)有限公司 一种核酸序列扩增和标记的方法

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IL307172A (en) 2023-11-01
WO2022212330A1 (en) 2022-10-06
JP2024512917A (ja) 2024-03-21
CA3214584A1 (en) 2022-10-06
BR112023019959A2 (pt) 2024-01-30
KR20230161955A (ko) 2023-11-28
US20240150753A1 (en) 2024-05-09
EP4314282A1 (en) 2024-02-07
AU2022246579A1 (en) 2023-09-21

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