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MX2020001675A - Metodo para detectar una mutacion en una planta de brassica oleracea. - Google Patents

Metodo para detectar una mutacion en una planta de brassica oleracea.

Info

Publication number
MX2020001675A
MX2020001675A MX2020001675A MX2020001675A MX2020001675A MX 2020001675 A MX2020001675 A MX 2020001675A MX 2020001675 A MX2020001675 A MX 2020001675A MX 2020001675 A MX2020001675 A MX 2020001675A MX 2020001675 A MX2020001675 A MX 2020001675A
Authority
MX
Mexico
Prior art keywords
brassica oleracea
oleracea plant
mutation
detect
aneuploidy
Prior art date
Application number
MX2020001675A
Other languages
English (en)
Inventor
Atsushi Izumida
Takao Suzuki
Tetsuya Hiramoto
Original Assignee
Sakata Seed Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sakata Seed Corp filed Critical Sakata Seed Corp
Publication of MX2020001675A publication Critical patent/MX2020001675A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • A01H1/045—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection using molecular markers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20—Brassicaceae, e.g. canola, broccoli or rucola
    • A01H6/203—Brassica oleraceae, e.g. broccoli or kohlrabi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00—Nucleic acid detection characterised by assay method
    • C12Q2561/113—Real time assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Physiology (AREA)
  • Plant Pathology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente descripción da a conocer un método para detectar un aneuploide de una planta de Brassica oleracea, que incluye: llevar a cabo una PCR en tiempo real usando ADN extraído de una muestra derivada de una planta de Brassica oleracea para ser probado como una plantilla y marcadores de ADN específicos para cada uno de dos o más cromosomas de la planta Brassica oleracea; y detectar aneuploidia cromosómica a partir de una diferencia relativa en la cantidad de amplificación. entre los marcadores de ADN obtenidos. De acuerdo con una modalidad de la presente invención, es posible detectar de manera simple, precisar y rápida una mutación (aneuploide cromosómico) que puede ocurrir en variedades de Brassica oleracea, en un laboratorio equipado con un equipo biológico molecular general en el curso de control de calidad de semillas e investigación de mejoramiento genético.
MX2020001675A 2017-08-17 2018-08-17 Metodo para detectar una mutacion en una planta de brassica oleracea. MX2020001675A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017157384 2017-08-17
PCT/JP2018/030486 WO2019035480A1 (ja) 2017-08-17 2018-08-17 ブラシカ・オレラセア植物の異型検出法

Publications (1)

Publication Number Publication Date
MX2020001675A true MX2020001675A (es) 2020-10-22

Family

ID=65362468

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020001675A MX2020001675A (es) 2017-08-17 2018-08-17 Metodo para detectar una mutacion en una planta de brassica oleracea.

Country Status (13)

Country Link
US (1) US11932912B2 (es)
EP (2) EP3670657A4 (es)
JP (2) JP7607402B2 (es)
KR (1) KR102801338B1 (es)
CN (1) CN111201318A (es)
AU (1) AU2018318559B2 (es)
BR (1) BR112020003129A2 (es)
CA (1) CA3073173A1 (es)
CL (1) CL2020000385A1 (es)
MX (1) MX2020001675A (es)
TW (1) TWI873082B (es)
WO (1) WO2019035480A1 (es)
ZA (1) ZA202001441B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI873082B (zh) * 2017-08-17 2025-02-21 日商莎卡達種子股份有限公司 甘藍（Ｂｒａｓｓｉｃａ ｏｌｅｒａｃｅａ）植物之異型檢出法
CN111705157B (zh) * 2020-07-24 2022-11-29 中国农业科学院蔬菜花卉研究所 用于甘蓝自交不亲和系ⅰ类s单元型筛选的pcr标记及引物
CN120380987B (zh) * 2025-06-30 2025-09-12 四川省农业科学院园艺研究所 提高辣椒花药组织培养胚状体诱导率、降低污染率的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0217406D0 (en) * 2002-07-26 2002-09-04 Syngenta Participations Ag Disease resistant brassicas
JP3836451B2 (ja) 2002-08-23 2006-10-25 京都府 トウガラシ属植物の辛味遺伝子座近傍に位置するｄｎａマーカーを用いた遺伝子型判別方法、ｄｎａマーカー検出に用いられるプライマー、およびこれを用いた遺伝子型判別方法
WO2009007091A2 (en) * 2007-07-09 2009-01-15 Bayer Bioscience N.V. Brassica plant comprising mutant fatty acyl-acp thioesterase alleles
CN102517390B (zh) * 2011-12-23 2013-11-13 厦门大学 基于实时荧光pcr的染色体非整倍体检测试剂盒
CN104531859B (zh) * 2014-12-18 2017-11-07 北京林业大学 一种检测非整倍体杂交子代植株的分子标记引物及非整倍体杂交子代植株的检测方法
TWI873082B (zh) * 2017-08-17 2025-02-21 日商莎卡達種子股份有限公司 甘藍（Ｂｒａｓｓｉｃａ ｏｌｅｒａｃｅａ）植物之異型檢出法

Also Published As

Publication number Publication date
EP4219758A1 (en) 2023-08-02
KR20200053500A (ko) 2020-05-18
ZA202001441B (en) 2021-07-28
AU2018318559B2 (en) 2024-11-28
US11932912B2 (en) 2024-03-19
EP3670657A1 (en) 2020-06-24
CL2020000385A1 (es) 2020-12-04
JP7607402B2 (ja) 2024-12-27
BR112020003129A2 (pt) 2020-08-04
RU2020110813A3 (es) 2022-04-06
RU2020110813A (ru) 2021-09-17
CN111201318A (zh) 2020-05-26
EP3670657A4 (en) 2021-06-02
JPWO2019035480A1 (ja) 2020-11-26
CA3073173A1 (en) 2019-02-21
KR102801338B1 (ko) 2025-04-30
TW201920684A (zh) 2019-06-01
AU2018318559A1 (en) 2020-03-05
WO2019035480A1 (ja) 2019-02-21
JP2023060024A (ja) 2023-04-27
TWI873082B (zh) 2025-02-21
US20210164057A1 (en) 2021-06-03

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