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MX2019001938A - Metodos para detectar aav. - Google Patents

Metodos para detectar aav.

Info

Publication number
MX2019001938A
MX2019001938A MX2019001938A MX2019001938A MX2019001938A MX 2019001938 A MX2019001938 A MX 2019001938A MX 2019001938 A MX2019001938 A MX 2019001938A MX 2019001938 A MX2019001938 A MX 2019001938A MX 2019001938 A MX2019001938 A MX 2019001938A
Authority
MX
Mexico
Prior art keywords
methods
determining
aav
particles
heterogeneity
Prior art date
Application number
MX2019001938A
Other languages
English (en)
Inventor
Jin Xiaoying
R O'riordan Catherine
Liu Lin
Zhang Kate
Original Assignee
Genzyme Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=59762045&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2019001938(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genzyme Corp filed Critical Genzyme Corp
Publication of MX2019001938A publication Critical patent/MX2019001938A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
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    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
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    • G—PHYSICS
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    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
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    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123—Virus like particles [VLP]
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
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    • G01—MEASURING; TESTING
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    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

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  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

En la presente divulgación se proporcionan métodos para determinar el serotipo de una partícula de virus y/o para determinar la heterogeneidad de una partícula de virus (p. ej., una partícula de AAV). En otras modalidades, la invención proporciona métodos para determinar la heterogeneidad de partículas de AAV. En algunos aspectos, la invención proporciona partículas virales (p. ej., partículas de rAAV) con estabilidad mejorada y/o eficiencia de la transducción mejorada mediante el incremento de la acetilación y/o desamidación de las proteínas de la cápside.
MX2019001938A 2016-08-15 2017-08-14 Metodos para detectar aav. MX2019001938A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662375314P 2016-08-15 2016-08-15
PCT/US2017/046814 WO2018035059A1 (en) 2016-08-15 2017-08-14 Methods for detecting aav

Publications (1)

Publication Number Publication Date
MX2019001938A true MX2019001938A (es) 2019-07-04

Family

ID=59762045

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019001938A MX2019001938A (es) 2016-08-15 2017-08-14 Metodos para detectar aav.
MX2023009081A MX2023009081A (es) 2016-08-15 2019-02-15 Metodos para detectar aav.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2023009081A MX2023009081A (es) 2016-08-15 2019-02-15 Metodos para detectar aav.

Country Status (23)

Country Link
US (5) US11698377B2 (es)
EP (2) EP3497207B1 (es)
JP (3) JP7021191B2 (es)
KR (3) KR102538037B1 (es)
CN (2) CN110168080B (es)
AU (2) AU2017312951B2 (es)
CA (1) CA3033856A1 (es)
CL (2) CL2019000392A1 (es)
CR (1) CR20190127A (es)
CY (1) CY1124364T1 (es)
DK (1) DK3497207T3 (es)
ES (1) ES2863674T3 (es)
HU (1) HUE053747T2 (es)
IL (3) IL264819B2 (es)
MA (1) MA55748A (es)
MX (2) MX2019001938A (es)
NZ (3) NZ751711A (es)
PH (1) PH12019500316A1 (es)
SG (2) SG10201913002QA (es)
TN (1) TN2019000047A1 (es)
TW (2) TWI781953B (es)
WO (1) WO2018035059A1 (es)
ZA (2) ZA201900945B (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201508026D0 (en) 2015-05-11 2015-06-24 Ucl Business Plc Capsid
ES2863674T3 (es) 2016-08-15 2021-10-11 Genzyme Corp Métodos para detectar AAV
JOP20190200A1 (ar) 2017-02-28 2019-08-27 Univ Pennsylvania تركيبات نافعة في معالجة ضمور العضل النخاعي
SI3589730T1 (sl) 2017-02-28 2024-04-30 The Trustees Of The University Of Pennsylvania Adeno-povezan virus (AAV) clade F vektor in njihova uporaba
MX2020008932A (es) * 2018-02-27 2020-10-01 Univ Pennsylvania Novedosos vectores de virus adenoasociado (aav), vectores de aav que tienen una desamidacion reducida de la capside y usos de estos.
MX2020008933A (es) * 2018-02-27 2021-01-15 Univ Pennsylvania Novedosos vectores de virus adenoasociado (aav), vectores de aav que tienen una desamidacion reducida de la capside y usos de estos.
EP3762500A1 (en) * 2018-03-06 2021-01-13 Voyager Therapeutics, Inc. Insect cell manufactured partial self-complementary aav genomes
CN112955239B (zh) * 2018-07-27 2022-11-29 沃特世科技公司 用于极性分子分析的液相色谱/质谱方法
US12195745B2 (en) * 2018-08-21 2025-01-14 Massachusetts Eye And Ear Infirmary Compositions and methods for modulating transduction efficiency of adeno-associated viruses
WO2020086893A1 (en) * 2018-10-25 2020-04-30 Regeneron Pharmaceuticals, Inc. Methods for analysis of viral capsid protein composition
WO2020210600A1 (en) * 2019-04-11 2020-10-15 Regenxbio Inc. Methods of size exclusion chromatography for the characterization of recombinant adeno-associated virus compositions
US11841352B2 (en) * 2019-07-31 2023-12-12 Thermo Finnigan Llc Determination and correction of retention time and mass/charge shifts in LC-MS experiments
EP4085255A1 (en) * 2020-01-03 2022-11-09 Sarepta Therapeutics, Inc. Methods for analyzing aav capsid proteins
AU2021213786A1 (en) 2020-01-29 2022-09-22 Genzyme Corporation Modified adeno-associated viral capsid proteins for ocular gene therapy and methods of use thereof
CN111517359B (zh) * 2020-04-23 2021-08-20 江南大学 一种手性硫化铜超粒子的合成方法
RU2748540C1 (ru) * 2021-02-08 2021-05-26 Автономная некоммерческая образовательная организация высшего образования "Сколковский институт науки и технологий" Способ детектирования вируса SARS-CoV-2 методом масс-спектрометрии
US20220268783A1 (en) * 2021-02-19 2022-08-25 Waters Technologies Corporation Methods for peptide mapping of adeno-associated virus (aav) proteins
JPWO2022224965A1 (es) * 2021-04-20 2022-10-27
IL309733A (en) * 2021-07-12 2024-02-01 Regeneron Pharma Methods for Viral Particle Characterization Using Liquid Chromatography-2D Mass Spectrometry
IL309735A (en) * 2021-07-12 2024-02-01 Regeneron Pharma Natural continuous mass spectrometry methods for viral particle screening
CN113552349B (zh) * 2021-07-30 2022-07-19 上海勉亦生物科技有限公司 Aav蛋白外壳的检测方法
WO2023132338A1 (ja) * 2022-01-06 2023-07-13 富士フイルム株式会社 タンパク質における糖が結合したアミノ酸部位を同定する方法、及びキット
WO2024186532A1 (en) * 2023-03-03 2024-09-12 Sartorius Bioanalytical Instruments, Inc. Methods for quantitating viral capsids
US20240418730A1 (en) * 2023-06-19 2024-12-19 Regeneron Pharmaceuticals, Inc. Host cell protein analysis for adeno-associated virus (aav)-based gene therapy
WO2025101795A1 (en) * 2023-11-08 2025-05-15 Oxford Biomedica (Us) Llc Deamidation depleted adeno-associated virus product

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5540931A (en) 1989-03-03 1996-07-30 Charles W. Hewitt Methods for inducing site-specific immunosuppression and compositions of site specific immunosuppressants
US6204059B1 (en) * 1994-06-30 2001-03-20 University Of Pittsburgh AAV capsid vehicles for molecular transfer
EP0931158A1 (en) 1996-09-06 1999-07-28 The Trustees Of The University Of Pennsylvania An inducible method for production of recombinant adeno-associated viruses utilizing t7 polymerase
US6566118B1 (en) 1997-09-05 2003-05-20 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6995006B2 (en) 1997-09-05 2006-02-07 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
EP2369002A1 (en) 1999-08-09 2011-09-28 Targeted Genetics Corporation Enhancement of expression of a single-stranded, heterologous nucleotide sequence from recombinant viral vectors by designing the sequence such that it forms intrastrand base pairs
ES2256265T3 (es) 2000-06-01 2006-07-16 University Of North Carolina At Chapel Hill Vectores de parvovirus duplicados.
US6723551B2 (en) 2001-11-09 2004-04-20 The United States Of America As Represented By The Department Of Health And Human Services Production of adeno-associated virus in insect cells
CA2406745C (en) 2001-11-13 2006-01-10 The Trustees Of The University Of Pennsylvania A method of detecting and/or identifying adeno-associated virus (aav) sequences and isolating novel sequences identified thereby
EP1496944B1 (en) 2002-05-01 2008-08-20 University of Florida Research Foundation, Inc. Improved raav expression systems for genetic modification of specific capsid proteins
EP1586107A2 (en) * 2002-11-22 2005-10-19 Caprion Pharmaceuticals, Inc. Constellation mapping and uses thereof
WO2004075861A2 (en) 2003-02-26 2004-09-10 Children's Hospital, Inc. Recombinant adeno-associated virus production
DE602004030327D1 (de) 2003-05-21 2011-01-13 Genzyme Corp Verfahren zur herstellung von präparationen rekombinanter aav-virionen, die weitgehend frei von leeren capsiden sind
EP1486567A1 (en) 2003-06-11 2004-12-15 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Improved adeno-associated virus (AAV) vector for gene therapy
US9441244B2 (en) * 2003-06-30 2016-09-13 The Regents Of The University Of California Mutant adeno-associated virus virions and methods of use thereof
US8283151B2 (en) 2005-04-29 2012-10-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Isolation, cloning and characterization of new adeno-associated virus (AAV) serotypes
WO2007046703A2 (en) * 2005-10-20 2007-04-26 Amsterdam Molecular Therapeutics B.V. Improved aav vectors produced in insect cells
EP2007795B1 (en) 2006-03-30 2016-11-16 The Board Of Trustees Of The Leland Stanford Junior University Aav capsid proteins
US20110275529A1 (en) 2008-09-19 2011-11-10 Charite Universitatsmedizin Berlin Identification and characterisation of recombinant viral gene therapy vectors
ES2634118T3 (es) 2009-02-11 2017-09-26 The University Of North Carolina At Chapel Hill Vectores de virus modificados y métodos para fabricar y utilizar los mismos
ES2881050T3 (es) 2009-06-16 2021-11-26 Genzyme Corp Métodos mejorados para la purificación de vectores de AAV recombinantes
ES2605305T3 (es) 2010-04-23 2017-03-13 University Of Massachusetts Vectores de AAV que se dirigen al SNC y métodos de uso de los mismos
WO2012031245A1 (en) * 2010-09-03 2012-03-08 North Carolina Central University Biodegradable liquogel and ph sensitive nanocarriers
US8663624B2 (en) 2010-10-06 2014-03-04 The Regents Of The University Of California Adeno-associated virus virions with variant capsid and methods of use thereof
KR101956885B1 (ko) 2011-02-17 2019-03-12 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 조직 특이성 변경 및 ａａｖ９-매개 유전자 전달 개선을 위한 조성물 및 방법
ES2857773T5 (es) 2011-08-24 2024-06-04 Univ Leland Stanford Junior Nuevas proteínas de la cápside de AAV para la transferencia de ácidos nucleicos
BR112014025985A2 (pt) * 2012-04-18 2017-07-11 Childrens Hospital Philadelphia composição e métodos para transferência de gene altamente eficaz com o uso de variantes de capsídeo de aav
US20140017716A1 (en) * 2012-07-11 2014-01-16 Siscapa Assay Technologies, Inc. Proteolytic digestion kit with dried reagents
GB201401707D0 (en) 2014-01-31 2014-03-19 Sec Dep For Health The Adeno-associated viral vectors
CN106459984B (zh) * 2014-03-10 2021-09-07 优尼科Ip有限公司 昆虫细胞中产生的进一步改善的aav载体
ES2863674T3 (es) 2016-08-15 2021-10-11 Genzyme Corp Métodos para detectar AAV

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JP2024023460A (ja) 2024-02-21
JP2022064980A (ja) 2022-04-26
WO2018035059A1 (en) 2018-02-22
TW201825898A (zh) 2018-07-16
RU2019107207A (ru) 2020-09-15
ES2863674T3 (es) 2021-10-11
MA55748A (fr) 2022-03-02
PH12019500316A1 (en) 2019-08-05
KR20240172773A (ko) 2024-12-10
BR112019002934A2 (pt) 2019-05-14
US20250027951A1 (en) 2025-01-23
TW202309068A (zh) 2023-03-01
NZ751711A (en) 2025-11-28
JP2019533803A (ja) 2019-11-21
KR20230078834A (ko) 2023-06-02
KR20190039253A (ko) 2019-04-10
KR102425289B1 (ko) 2022-07-27
US20210041451A1 (en) 2021-02-11
US20250138024A1 (en) 2025-05-01
US20240044910A1 (en) 2024-02-08
AU2017312951A1 (en) 2019-04-04
ZA201900945B (en) 2021-06-30
KR102738219B1 (ko) 2024-12-05
MX2023009081A (es) 2023-08-08
JP7021191B2 (ja) 2022-02-16
CN110168080A (zh) 2019-08-23
CR20190127A (es) 2019-06-25
TWI781953B (zh) 2022-11-01
CN118688363A (zh) 2024-09-24
HUE053747T2 (hu) 2021-07-28
US12298313B1 (en) 2025-05-13
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