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MX2018005831A - Gradientes de ph-sal opuestos para separaciones de proteina mejoradas. - Google Patents

Gradientes de ph-sal opuestos para separaciones de proteina mejoradas.

Info

Publication number
MX2018005831A
MX2018005831A MX2018005831A MX2018005831A MX2018005831A MX 2018005831 A MX2018005831 A MX 2018005831A MX 2018005831 A MX2018005831 A MX 2018005831A MX 2018005831 A MX2018005831 A MX 2018005831A MX 2018005831 A MX2018005831 A MX 2018005831A
Authority
MX
Mexico
Prior art keywords
fragments
opposite
improved protein
protein separations
salt gradients
Prior art date
Application number
MX2018005831A
Other languages
English (en)
Inventor
Joehnck Matthias
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of MX2018005831A publication Critical patent/MX2018005831A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3861—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus
    • B01D15/388—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography using an external stimulus modifying the pH
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método para la separación preparativa mejorada de proteínas, particularmente anticuerpos monoclonales (mAB) de sus variantes de carga asociadas (por ejemplo, monómeros ácidos y básicos), variantes de glicosilación, y/o variantes de tamaño solubles (por ejemplo, agregados, monómeros, fragmentos 2/3, fragmentos 3/4, fragmentos de unión a antígeno (Fab) y fragmentos cristalizables (Fc).
MX2018005831A 2015-11-18 2016-10-28 Gradientes de ph-sal opuestos para separaciones de proteina mejoradas. MX2018005831A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15195243 2015-11-18
PCT/EP2016/001804 WO2017084738A1 (en) 2015-11-18 2016-10-28 Opposite ph-salt gradients for improved protein separations

Publications (1)

Publication Number Publication Date
MX2018005831A true MX2018005831A (es) 2018-08-01

Family

ID=54608367

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018005831A MX2018005831A (es) 2015-11-18 2016-10-28 Gradientes de ph-sal opuestos para separaciones de proteina mejoradas.

Country Status (13)

Country Link
US (1) US20180346510A1 (es)
EP (1) EP3377514A1 (es)
JP (1) JP2018537458A (es)
KR (1) KR20180081605A (es)
CN (1) CN108350027A (es)
AU (1) AU2016356482A1 (es)
BR (1) BR112018009882A2 (es)
CA (1) CA3005484A1 (es)
IL (1) IL259181A (es)
MX (1) MX2018005831A (es)
RU (1) RU2018121657A (es)
SG (1) SG11201804083QA (es)
WO (1) WO2017084738A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180083409A (ko) * 2015-11-18 2018-07-20 메르크 파텐트 게엠베하 이온 교환 크로마토그래피에서의 개선된 단백질 분리
CN109293734A (zh) * 2018-09-29 2019-02-01 上海药明生物技术有限公司 一种消除层析中洗脱肩峰的方法
WO2020172658A1 (en) * 2019-02-24 2020-08-27 Bristol-Myers Squibb Company Methods of isolating a protein
US11022585B2 (en) * 2019-06-09 2021-06-01 Dionex Corporation Methods and systems for optimizing buffer conditions with liquid chromatography
CN114014906B (zh) * 2020-06-24 2024-01-12 夏尔巴生物技术(苏州)有限公司 一种利用阳离子交换层析纯化疏水性蛋白的方法
CN114544839B (zh) * 2022-01-20 2024-08-20 未名生物医药有限公司 一种抗人神经生长因子抗体的电荷变异体检测方法
WO2024261724A1 (en) * 2023-06-23 2024-12-26 Lupin Limited Ion-exchange chromatography for separation and analysis of charge variants

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1308455T1 (sl) * 1998-05-06 2006-08-31 Genentech Inc Sestavek, ki obsega protitelesa anti-HER2
CN1234725C (zh) * 2004-04-07 2006-01-04 陈志南 一种高效快速纯化制备片段抗体的方法
TWI391399B (zh) * 2005-05-25 2013-04-01 Hoffmann La Roche 測定溶離多肽之鹽濃度之方法
ES2596727T3 (es) * 2010-03-30 2017-01-11 Octapharma Ag Procedimiento de purificación de un factor proteico de crecimiento G-CSF
EP2718306A1 (en) * 2011-06-13 2014-04-16 Merck Sharp & Dohme Corp. Methods of purification of native or mutant forms of diphtheria toxin
US9334319B2 (en) * 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
BR112015005161A2 (pt) * 2012-09-11 2017-07-04 Coherus Biosciences Inc etanercepte corretamente dobrado em alta pureza e excelente rendimento
JP2015537212A (ja) * 2012-11-15 2015-12-24 ジェネンテック， インコーポレイテッド イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー
CN104628846B (zh) * 2013-11-06 2019-12-06 三生国健药业（上海）股份有限公司 重组蛋白质的纯化方法
ES2612427T3 (es) * 2014-02-11 2017-05-16 Richter-Helm Bio Tec Gmbh & Co. Kg Método para purificar teriparatida (PTH1-34)

Also Published As

Publication number Publication date
AU2016356482A1 (en) 2018-06-28
SG11201804083QA (en) 2018-06-28
IL259181A (en) 2018-07-31
KR20180081605A (ko) 2018-07-16
BR112018009882A2 (pt) 2018-11-13
EP3377514A1 (en) 2018-09-26
JP2018537458A (ja) 2018-12-20
CN108350027A (zh) 2018-07-31
CA3005484A1 (en) 2017-05-26
US20180346510A1 (en) 2018-12-06
RU2018121657A3 (es) 2020-01-31
WO2017084738A1 (en) 2017-05-26
RU2018121657A (ru) 2019-12-19

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