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MX2017002234A - Metodos de cultivo semicontinuo. - Google Patents

Metodos de cultivo semicontinuo.

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Publication number
MX2017002234A
MX2017002234A MX2017002234A MX2017002234A MX2017002234A MX 2017002234 A MX2017002234 A MX 2017002234A MX 2017002234 A MX2017002234 A MX 2017002234A MX 2017002234 A MX2017002234 A MX 2017002234A MX 2017002234 A MX2017002234 A MX 2017002234A
Authority
MX
Mexico
Prior art keywords
microorganisms
culture methods
crop
container
carbon
Prior art date
Application number
MX2017002234A
Other languages
English (en)
Other versions
MX381412B (es
Inventor
E Armenta Roberto
Berryman Kevin
Sun Zhiyong
Milway Michael
VALENTINE Mercia
Purdue Laura
Original Assignee
Mara Renewables Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mara Renewables Corp filed Critical Mara Renewables Corp
Publication of MX2017002234A publication Critical patent/MX2017002234A/es
Publication of MX381412B publication Critical patent/MX381412B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6431—Linoleic acids [18:2[n-6]]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

En la presente se presentan métodos para cultivar un microorganismo. Los métodos incluyen proporcionar un recipiente que comprende uno o más microorganismos en un medio que tiene una primera relación carbono/nitrógeno; cultivar los microorganismos hasta que el cultivo alcance un indicador umbral; cosechar una parte del cultivo mientras mantiene la mayoría del cultivo en el recipiente; y agregar medio recién preparado que comprende una segunda relación de carbono/nitrógeno respecto al recipiente con la mayoría del cultivo que comprende los microorganismos.
MX2017002234A 2014-10-16 2015-10-12 Métodos de cultivo semicontinuo. MX381412B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462064668P 2014-10-16 2014-10-16
PCT/IB2015/057807 WO2016059540A1 (en) 2014-10-16 2015-10-12 Semi-continuous culture methods

Publications (2)

Publication Number Publication Date
MX2017002234A true MX2017002234A (es) 2017-09-15
MX381412B MX381412B (es) 2025-03-12

Family

ID=55746207

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017002234A MX381412B (es) 2014-10-16 2015-10-12 Métodos de cultivo semicontinuo.

Country Status (16)

Country Link
US (4) US10676775B2 (es)
EP (2) EP3207164B1 (es)
JP (1) JP6806297B2 (es)
KR (1) KR102501031B1 (es)
CN (1) CN106687605B (es)
AU (3) AU2015332094B2 (es)
BR (1) BR112017003556B1 (es)
CA (1) CA2959860C (es)
CL (1) CL2017000891A1 (es)
DK (1) DK3207164T3 (es)
ES (1) ES2856838T3 (es)
IL (1) IL250275A0 (es)
MX (1) MX381412B (es)
NZ (1) NZ728653A (es)
PT (1) PT3207164T (es)
WO (1) WO2016059540A1 (es)

Families Citing this family (8)

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KR102501031B1 (ko) 2014-10-16 2023-02-16 마라 리뉴어블즈 코퍼레이션 반연속적 배양 방법
US10851395B2 (en) 2016-06-10 2020-12-01 MARA Renewables Corporation Method of making lipids with improved cold flow properties
KR101956440B1 (ko) * 2017-02-10 2019-06-24 부경대학교 산학협력단 반연속 배양 방법을 이용한 바이러스의 생산 방법
CN107805649A (zh) * 2017-11-10 2018-03-16 浙江帝斯曼中肯生物科技有限公司 一种生产结冷胶的新型发酵工艺
CN109371071B (zh) * 2018-05-17 2022-11-04 梁云 Dha微生物油脂中脂肪酸组合物成分调整的方法
CN109136308B (zh) * 2018-09-26 2021-01-26 武汉中科光谷绿色生物技术有限公司 一种提高发酵产聚唾液酸的方法及发酵液
CN109161570B (zh) * 2018-09-26 2020-12-18 武汉中科光谷绿色生物技术有限公司 一种提高发酵产n-乙酰神经氨酸的方法及发酵液
CN116064409A (zh) * 2023-01-06 2023-05-05 四川阿思科力生物科技有限公司 一种大规模高产量细胞连续培养和收获的生产工艺

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CA2960450C (en) 2014-10-16 2023-02-14 MARA Renewables Corporation Repeated fed-batch culture methods

Also Published As

Publication number Publication date
KR20170068461A (ko) 2017-06-19
BR112017003556A2 (pt) 2018-02-27
WO2016059540A1 (en) 2016-04-21
KR102501031B1 (ko) 2023-02-16
US20160108452A1 (en) 2016-04-21
CA2959860C (en) 2023-04-18
CN106687605B (zh) 2022-02-01
PT3207164T (pt) 2021-02-05
MX381412B (es) 2025-03-12
US12410458B2 (en) 2025-09-09
IL250275A0 (en) 2017-03-30
AU2021218000A1 (en) 2021-09-02
AU2023219925A1 (en) 2023-09-14
US11345943B2 (en) 2022-05-31
US10676775B2 (en) 2020-06-09
EP3839041A1 (en) 2021-06-23
DK3207164T3 (da) 2021-02-08
AU2023219925B2 (en) 2025-10-02
JP2017530699A (ja) 2017-10-19
US20220356506A1 (en) 2022-11-10
ES2856838T3 (es) 2021-09-28
EP3207164B1 (en) 2021-01-20
AU2015332094A1 (en) 2017-02-16
AU2015332094B2 (en) 2021-05-20
CA2959860A1 (en) 2016-04-21
CN106687605A (zh) 2017-05-17
NZ728653A (en) 2023-07-28
EP3207164A4 (en) 2018-04-18
US20200332338A1 (en) 2020-10-22
AU2021218000B2 (en) 2023-06-15
JP6806297B2 (ja) 2021-01-06
EP3207164A1 (en) 2017-08-23
BR112017003556B1 (pt) 2023-10-03
US20250154552A1 (en) 2025-05-15
CL2017000891A1 (es) 2017-11-03

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