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MX2017001124A - Metodo y aparato para rotura de celulas de biomasa. - Google Patents

Metodo y aparato para rotura de celulas de biomasa.

Info

Publication number
MX2017001124A
MX2017001124A MX2017001124A MX2017001124A MX2017001124A MX 2017001124 A MX2017001124 A MX 2017001124A MX 2017001124 A MX2017001124 A MX 2017001124A MX 2017001124 A MX2017001124 A MX 2017001124A MX 2017001124 A MX2017001124 A MX 2017001124A
Authority
MX
Mexico
Prior art keywords
pressure
kpa
suspension
biomass material
pressurized
Prior art date
Application number
MX2017001124A
Other languages
English (en)
Inventor
D'hondt Els
Elst Kathy
Günerken Emre
Garcia-Gonzalez Linsey
H Wijffels René
H M Eppink Michel
Original Assignee
Vito Nv (Vlaamse Instelling Voor Tech Onderzoek Nv)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vito Nv (Vlaamse Instelling Voor Tech Onderzoek Nv) filed Critical Vito Nv (Vlaamse Instelling Voor Tech Onderzoek Nv)
Publication of MX2017001124A publication Critical patent/MX2017001124A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Accessories For Mixers (AREA)

Abstract

La presente invención se refiere a un método para romper el material de biomasa suspendido que comprende los pasos de: proporcionar una suspensión del material de biomasa en un primer recipiente (1), dicha suspensión que tiene un peso seco de células que está comprendido entre 0.1% y 15% (peso/peso); presurizar dicha suspensión a una primera presión (p1) que está comprendida entre 7500 kPa y 450000 kPa utilizando una primera bomba (10) de alta presión; proporcionar un agente de rotura gaseoso presurizado y/o un agente de rotura líquido presurizado en un segundo recipiente (2); presurizar dicho agente de rotura gaseoso y/o líquido a una segunda presión (p2) que está comprendida entre 7500 kPa y 450000 kPa utilizando una segunda bomba (20) de alta presión, dicha segunda presión (p2) que es mayor que dicha primera presión (p1); inyectar una corriente de dicho agente de rotura presurizado en una corriente de dicha suspensión presurizada del material de biomasa y formar una microemulsión; hacer pasar dicha microemulsión secuencialmente a través de un sistema (11) de agitación hidrodinámica y una válvula (13) de alivio; también se proporciona un aparato (y la pila de sistemas (paralelos)) para romper el material de biomasa suspendido.
MX2017001124A 2014-07-25 2015-07-24 Metodo y aparato para rotura de celulas de biomasa. MX2017001124A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14178628.5A EP2977439B1 (en) 2014-07-25 2014-07-25 Method and apparatus for disruption of biomass cells
PCT/EP2015/067056 WO2016012613A1 (en) 2014-07-25 2015-07-24 Method and apparatus for disruption of biomass cells

Publications (1)

Publication Number Publication Date
MX2017001124A true MX2017001124A (es) 2017-09-01

Family

ID=51257320

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017001124A MX2017001124A (es) 2014-07-25 2015-07-24 Metodo y aparato para rotura de celulas de biomasa.

Country Status (11)

Country Link
EP (2) EP2977439B1 (es)
JP (1) JP6609620B2 (es)
CN (1) CN106536713A (es)
CA (1) CA2954737A1 (es)
DK (1) DK2977439T3 (es)
ES (1) ES2626963T3 (es)
IL (1) IL249840A0 (es)
MX (1) MX2017001124A (es)
PT (1) PT2977439T (es)
SG (1) SG11201700570VA (es)
WO (1) WO2016012613A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107175242B (zh) * 2017-07-07 2019-12-13 湖南大学 厌氧培养瓶充气抽真空清洗系统
BR102018004973B1 (pt) * 2018-03-13 2021-10-13 Petróleo Brasileiro S.A. - Petrobras Dispositivo e método para rompimento de células de microorganismos por extrusão
DE102018118021B4 (de) * 2018-07-25 2025-07-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren sowie Vorrichtung zum Aufbereiten von Zellsuspensionen
US12446573B2 (en) * 2021-02-01 2025-10-21 GPS Innovations Tunable, simple, higher-yield, higher-rate, lower-cost recovery method of biopharmaceutical products from cell factories
CN115228516B (zh) * 2021-04-23 2024-06-14 中国石油化工股份有限公司 用于调控多相体系中分散相尺寸分布的限域单元和方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57150379A (en) * 1981-03-14 1982-09-17 Kikujiro Ishiwatari Crushing of cell membrane of chlorella
JPH0661260B2 (ja) * 1986-06-05 1994-08-17 三菱重工業株式会社 菌体の粉砕法
WO1991001367A1 (en) * 1989-07-20 1991-02-07 Bioeng, Inc. Supercritical fluid disruption of and extraction from microbial cells
US5306637A (en) 1991-08-22 1994-04-26 Lin Ho Mu Method for recovery of intracellular material by disruption of microbial cells with carbon dioxide under pressure
JPH1142440A (ja) * 1997-07-28 1999-02-16 Jiinasu:Kk クロレラの破砕方法およびクロレラ破砕物並びにその安定化方法、および安定化されたクロレラ破砕物
JP2002078478A (ja) * 2000-09-05 2002-03-19 Shimadzu Corp 細胞処理方法、細胞処理装置及び細胞から取り出された物質
US7238522B2 (en) * 2003-05-30 2007-07-03 Advisys, Inc. Devices and methods for biomaterial production
WO2008005537A2 (en) 2006-07-07 2008-01-10 Tyco Healthcare Group Lp Mailbox style sharps container
DE102008036723A1 (de) * 2008-08-07 2010-02-25 Uhde High Pressure Technologies Gmbh Zellaufschluss pflanzlicher oder tierischer Ausgangsmaterialien mittels Kombination von Sprühverfahren und Dekompression zur selektiven Extraktion und Abscheidung intrazellulärer Wertstoffe
JP5232976B2 (ja) * 2009-02-18 2013-07-10 愛知県 バイオマス粉砕方法及びバイオマス粉砕装置並びに糖類製造方法
JP2010279292A (ja) * 2009-06-04 2010-12-16 Toshiba Plant Systems & Services Corp 細胞破砕装置および細胞破砕方法

Also Published As

Publication number Publication date
CN106536713A (zh) 2017-03-22
WO2016012613A1 (en) 2016-01-28
EP3172311A1 (en) 2017-05-31
DK2977439T3 (en) 2017-06-12
CA2954737A1 (en) 2016-01-28
JP2017526348A (ja) 2017-09-14
ES2626963T3 (es) 2017-07-26
EP3172311B1 (en) 2019-10-02
EP2977439B1 (en) 2017-03-01
EP2977439A1 (en) 2016-01-27
JP6609620B2 (ja) 2019-11-20
IL249840A0 (en) 2017-03-30
PT2977439T (pt) 2017-06-09
SG11201700570VA (en) 2017-02-27

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