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MX2016015789A - Deteccion de endosporas mediante el uso de material de recoleccion hidrofobico. - Google Patents

Deteccion de endosporas mediante el uso de material de recoleccion hidrofobico.

Info

Publication number
MX2016015789A
MX2016015789A MX2016015789A MX2016015789A MX2016015789A MX 2016015789 A MX2016015789 A MX 2016015789A MX 2016015789 A MX2016015789 A MX 2016015789A MX 2016015789 A MX2016015789 A MX 2016015789A MX 2016015789 A MX2016015789 A MX 2016015789A
Authority
MX
Mexico
Prior art keywords
endospores
carrier medium
lanthanide
endosporas
detection
Prior art date
Application number
MX2016015789A
Other languages
English (en)
Other versions
MX375915B (es
Inventor
H Banks Rodney
Richard Ortmann Nathan
Erickson Anthony
Patricia BLACK Elaine
Original Assignee
Ecolab Usa Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecolab Usa Inc filed Critical Ecolab Usa Inc
Publication of MX2016015789A publication Critical patent/MX2016015789A/es
Publication of MX375915B publication Critical patent/MX375915B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Análisis óptico in situ de endosporas bacterianas pueden ser inhibido cuando las endosporas están presentes dentro de un medio portador ópticamente activo. Para ayudar a aislar las endosporas a partir del medio portador, en algunos ejemplos, el medio portador es pasado a través de un material hidrofóbico que captura las endosporas por medio de atracción hidrofóbica. Subsecuentemente, un fluido de germinación y fuente de lantánido, como terbio, pueden ser agregados a las endosporas bacterianas capturadas en el material hidrofóbico para formar un complejo de lantanido-ácido dipicolínico en el fluido de germinación. El fluido de germinación puede entonces ser analizado ópticamente por medir la respuesta fluorométrica del complejo de lantánido-ácido dipicolínico para determinar una concentración de las endosporas en el medio portador.
MX2016015789A 2014-06-26 2015-06-26 Deteccion de endosporas mediante el uso de material de recoleccion hidrofobico. MX375915B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/315,606 US10316347B2 (en) 2014-06-26 2014-06-26 Endospore detection using hydrophobic collection material
PCT/US2015/038005 WO2015200807A1 (en) 2014-06-26 2015-06-26 Endospore detection using hydrophobic collection material

Publications (2)

Publication Number Publication Date
MX2016015789A true MX2016015789A (es) 2017-04-25
MX375915B MX375915B (es) 2025-03-07

Family

ID=54929870

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016015789A MX375915B (es) 2014-06-26 2015-06-26 Deteccion de endosporas mediante el uso de material de recoleccion hidrofobico.

Country Status (7)

Country Link
US (1) US10316347B2 (es)
EP (1) EP3161486B1 (es)
CN (1) CN106461660B (es)
AU (1) AU2015279644B2 (es)
CA (1) CA2947498C (es)
MX (1) MX375915B (es)
WO (1) WO2015200807A1 (es)

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* Cited by examiner, † Cited by third party
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Also Published As

Publication number Publication date
CA2947498C (en) 2022-09-13
CN106461660B (zh) 2019-10-18
US20150376675A1 (en) 2015-12-31
MX375915B (es) 2025-03-07
EP3161486A1 (en) 2017-05-03
AU2015279644A1 (en) 2016-11-10
WO2015200807A1 (en) 2015-12-30
EP3161486B1 (en) 2023-08-09
CN106461660A (zh) 2017-02-22
AU2015279644B2 (en) 2021-08-12
CA2947498A1 (en) 2015-12-30
EP3161486A4 (en) 2017-12-27
US10316347B2 (en) 2019-06-11

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