MX2016010680A - Perfeccionamiento de expresión de proteína recombinante con el uso de un sistema de retención de célula basado en membrana. - Google Patents
Perfeccionamiento de expresión de proteína recombinante con el uso de un sistema de retención de célula basado en membrana.Info
- Publication number
- MX2016010680A MX2016010680A MX2016010680A MX2016010680A MX2016010680A MX 2016010680 A MX2016010680 A MX 2016010680A MX 2016010680 A MX2016010680 A MX 2016010680A MX 2016010680 A MX2016010680 A MX 2016010680A MX 2016010680 A MX2016010680 A MX 2016010680A
- Authority
- MX
- Mexico
- Prior art keywords
- retention system
- cell
- membrane
- enhancement
- protein expression
- Prior art date
Links
- 230000014759 maintenance of location Effects 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 2
- 239000012528 membrane Substances 0.000 title abstract 2
- 238000004113 cell culture Methods 0.000 abstract 2
- 230000015271 coagulation Effects 0.000 abstract 1
- 238000005345 coagulation Methods 0.000 abstract 1
- 239000000835 fiber Substances 0.000 abstract 1
- 230000010412 perfusion Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000005086 pumping Methods 0.000 abstract 1
- 108010013773 recombinant FVIIa Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2511/00—Cells for large scale production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Peptides Or Proteins (AREA)
Abstract
Se trata del uso innovador de un sistema de retención de célula basado en membrana externa conjuntamente con cultivo celular de perfusión para expresión celular mejorada de proteínas recombinantes, particularmente proteínas de coagulación, tales como rFVIII, rFVIII con Dominio B Eliminado, rFIX o rFVII/rFVIIa. El uso de tal sistema en alta densidad de celula resulta en un cultivo celular más homogéneo debido a las fuerzas mecánicas inducidas durante la operación del sistema de retención, tales como la circulación celular inducida por bombeo a través de las fibras, además que la retención de algunos componentes celulares y de medio permite un ambiente ideal para las células. El uso del método proporcionado resulta en mejoras en productividad celular y en productividad volumétrica. El sistema de retención de célula basado en membrana es descartable y cuando es conectado a un biorreactor descartable, el mismo permite que todo el sistema de perfusión sea descartable cuando el medio y la recolección son desechados en sacos descartables. El sistema biorreactor de perfusión descartable puede ser operado como un sistema cerrado y no requiere limpieza, vaporización o el uso de una instalación con tubería rígida.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461940493P | 2014-02-17 | 2014-02-17 | |
| PCT/BR2015/000019 WO2015120527A2 (en) | 2014-02-17 | 2015-02-12 | Enhancement of recombinant protein expression using a membrane-based cell retention system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2016010680A true MX2016010680A (es) | 2017-04-27 |
Family
ID=53800716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2016010680A MX2016010680A (es) | 2014-02-17 | 2015-02-12 | Perfeccionamiento de expresión de proteína recombinante con el uso de un sistema de retención de célula basado en membrana. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20170009269A1 (es) |
| EP (1) | EP3107934A4 (es) |
| JP (1) | JP2017506077A (es) |
| KR (1) | KR20160138403A (es) |
| CN (1) | CN106414490A (es) |
| AU (1) | AU2015218193A1 (es) |
| BR (1) | BR112016018797A8 (es) |
| CA (1) | CA2939872A1 (es) |
| CL (1) | CL2016002068A1 (es) |
| MX (1) | MX2016010680A (es) |
| WO (1) | WO2015120527A2 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017509659A (ja) | 2014-04-01 | 2017-04-06 | アドヴァンテック・バイオサイエンス・ファルマスーティカ・リミターダ | 低糖−低グリシンの安定な第ｖｉｉｉ因子製剤 |
| JP2017509658A (ja) | 2014-04-01 | 2017-04-06 | アドヴァンテック・バイオサイエンス・ファルマスーティカ・リミターダ | カルシウム添加剤を使用しない第ｖｉｉｉ因子の安定化 |
| CN111344558A (zh) | 2017-10-06 | 2020-06-26 | 龙沙有限公司 | 使用拉曼光谱法自动控制细胞培养 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6200560B1 (en) * | 1998-10-20 | 2001-03-13 | Avigen, Inc. | Adeno-associated virus vectors for expression of factor VIII by target cells |
| US20040229335A1 (en) * | 2003-05-15 | 2004-11-18 | Introgen Therapeutics, Inc. | Methods and compositions for the production of adenoviral vectors |
| CN104388324A (zh) * | 2004-03-05 | 2015-03-04 | 帝斯曼知识产权资产管理有限公司 | 用于通过连续灌注和交互切向流来培养细胞的方法 |
| WO2009018307A2 (en) * | 2007-07-31 | 2009-02-05 | Wyeth | Analysis of polypeptide production |
| PL2501822T3 (pl) * | 2009-11-17 | 2017-12-29 | E. R. Squibb & Sons, L.L.C. | Sposoby zwiększenia produkcji białek |
| US8668886B2 (en) * | 2011-04-24 | 2014-03-11 | Therapeutic Proteins International, LLC | Separative bioreactor |
| KR20160138477A (ko) * | 2014-03-23 | 2016-12-05 | 어드밴텍 바이오사이언스 파마큐티카 엘티디에이 | 구리를 이용한 재조합 단백질의 발현 증가 방법 |
-
2015
- 2015-02-12 KR KR1020167025573A patent/KR20160138403A/ko not_active Withdrawn
- 2015-02-12 CA CA2939872A patent/CA2939872A1/en not_active Abandoned
- 2015-02-12 JP JP2016553414A patent/JP2017506077A/ja not_active Withdrawn
- 2015-02-12 US US15/119,202 patent/US20170009269A1/en not_active Abandoned
- 2015-02-12 CN CN201580019901.8A patent/CN106414490A/zh active Pending
- 2015-02-12 AU AU2015218193A patent/AU2015218193A1/en not_active Abandoned
- 2015-02-12 WO PCT/BR2015/000019 patent/WO2015120527A2/en not_active Ceased
- 2015-02-12 EP EP15748442.9A patent/EP3107934A4/en not_active Withdrawn
- 2015-02-12 BR BR112016018797A patent/BR112016018797A8/pt not_active Application Discontinuation
- 2015-02-12 MX MX2016010680A patent/MX2016010680A/es unknown
-
2016
- 2016-08-17 CL CL2016002068A patent/CL2016002068A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CA2939872A1 (en) | 2015-08-20 |
| BR112016018797A8 (pt) | 2020-06-23 |
| CL2016002068A1 (es) | 2017-08-04 |
| US20170009269A1 (en) | 2017-01-12 |
| BR112016018797A2 (pt) | 2017-08-08 |
| CN106414490A (zh) | 2017-02-15 |
| JP2017506077A (ja) | 2017-03-02 |
| KR20160138403A (ko) | 2016-12-05 |
| AU2015218193A1 (en) | 2016-10-06 |
| WO2015120527A3 (en) | 2015-12-10 |
| EP3107934A4 (en) | 2017-10-18 |
| WO2015120527A2 (en) | 2015-08-20 |
| EP3107934A2 (en) | 2016-12-28 |
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