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MX2016009328A - Metodos para usar capsaicina sintasa para la produccion microbiana de capsaicinoides. - Google Patents

Metodos para usar capsaicina sintasa para la produccion microbiana de capsaicinoides.

Info

Publication number
MX2016009328A
MX2016009328A MX2016009328A MX2016009328A MX2016009328A MX 2016009328 A MX2016009328 A MX 2016009328A MX 2016009328 A MX2016009328 A MX 2016009328A MX 2016009328 A MX2016009328 A MX 2016009328A MX 2016009328 A MX2016009328 A MX 2016009328A
Authority
MX
Mexico
Prior art keywords
capsaicinoids
methods
microbial production
capsaicin synthase
gene product
Prior art date
Application number
MX2016009328A
Other languages
English (en)
Inventor
Chen Hui
Wang Hongxue
YU Oliver
Original Assignee
Conagen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Conagen Inc filed Critical Conagen Inc
Publication of MX2016009328A publication Critical patent/MX2016009328A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/16—Amides, e.g. hydroxamic acids
    • A61K31/165—Amides, e.g. hydroxamic acids having aromatic rings, e.g. colchicine, atenolol, progabide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01001—Acetate-CoA ligase (6.2.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Se describe un método biosintético para hacer un capsaicinoide que incluye expresar un primer producto génico de CS/AT3/Pun1 en un sistema celular, hacer crecer el sistema celular en un medio, y recolectar el capsaicinoide. El método biosintético incluye además expresar un segundo producto génico de ACS1 y expresar un tercer producto génico de pAMT en el sistema celular.
MX2016009328A 2014-01-17 2015-01-16 Metodos para usar capsaicina sintasa para la produccion microbiana de capsaicinoides. MX2016009328A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461928803P 2014-01-17 2014-01-17
PCT/US2015/011729 WO2015109168A1 (en) 2014-01-17 2015-01-16 Methods of using capsaicin synthase for the microbial production of capsaicinoids

Publications (1)

Publication Number Publication Date
MX2016009328A true MX2016009328A (es) 2017-04-27

Family

ID=53543469

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016009328A MX2016009328A (es) 2014-01-17 2015-01-16 Metodos para usar capsaicina sintasa para la produccion microbiana de capsaicinoides.

Country Status (12)

Country Link
US (1) US9951358B2 (es)
EP (1) EP3094320B1 (es)
JP (1) JP6611359B2 (es)
KR (1) KR20160125370A (es)
CN (1) CN106456588B (es)
AU (1) AU2015206358A1 (es)
BR (1) BR112016016508A2 (es)
CA (1) CA2937124A1 (es)
HU (1) HUE063097T2 (es)
MX (1) MX2016009328A (es)
PL (1) PL3094320T3 (es)
WO (1) WO2015109168A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160125370A (ko) 2014-01-17 2016-10-31 코나겐 인크. 캡사이시노이드의 미생물적 생산을 위한 캡사이신 신타제의 사용 방법
CN104962595B (zh) * 2015-05-25 2018-11-27 广州美格生物科技有限公司 一种可用于胚胎注射制备敲除小鼠的Cas9蛋白的制备方法
KR20180101445A (ko) * 2016-01-07 2018-09-12 코나겐 인크. 생합성 프로세스에 의해 캡시노이드를 제조하는 방법
US11459591B2 (en) * 2016-07-19 2022-10-04 Conagen Inc. Method for the microbial production of specific natural capsaicinoids
CN110305031B (zh) * 2019-07-03 2022-07-12 遂宁晶安科技有限公司 辣椒素的制备方法及利用该方法制备得到的辣椒素
CN113461793B (zh) * 2021-06-30 2023-12-22 华南农业大学 辣椒ERF转录因子CaERF102及其在提高辣椒素含量中的应用
CN115960736B (zh) * 2023-03-01 2024-05-28 石河子大学 一种产香草胺和辣椒碱的酿酒酵母工程菌及其构建方法与应用
CN116837016B (zh) * 2023-08-29 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种构建生产辣椒素香草壬酰胺的重组大肠杆菌工程菌株的方法及重组菌株和应用
CN118956756B (zh) * 2024-09-29 2025-07-25 中国人民解放军空军军医大学 一种痛感受器皮肤类器官芯片及其培养基、构建方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4043605B2 (ja) * 1998-07-02 2008-02-06 丸善製薬株式会社 カプサイシン類縁体の製造法
JP3951008B2 (ja) * 2002-01-17 2007-08-01 国立大学法人 岡山大学 カプサイシン分解合成酵素及びその生産方法
US20040033530A1 (en) * 2002-04-08 2004-02-19 Awrey Donald E. High throughput purification, characterization and identification of recombinant proteins
CN102065680B (zh) * 2008-06-23 2014-12-24 味之素株式会社 能生物合成辣椒素酯类物质的基因修饰植物
CN103842509B (zh) 2011-07-13 2016-06-08 加拿大国家研究委员会 用于烷酰基辅酶a合成的基因和蛋白质
CN103725652B (zh) 2013-12-25 2016-06-29 无锡新和源发酵技术研究院有限公司 一种酰基辅酶a合成酶及其应用
KR20160125370A (ko) 2014-01-17 2016-10-31 코나겐 인크. 캡사이시노이드의 미생물적 생산을 위한 캡사이신 신타제의 사용 방법

Also Published As

Publication number Publication date
HUE063097T2 (hu) 2023-12-28
EP3094320C0 (en) 2023-06-14
JP2017507648A (ja) 2017-03-23
US9951358B2 (en) 2018-04-24
EP3094320A4 (en) 2017-09-27
CN106456588A (zh) 2017-02-22
CN106456588B (zh) 2021-04-30
EP3094320B1 (en) 2023-06-14
BR112016016508A2 (pt) 2017-10-03
AU2015206358A1 (en) 2016-09-01
JP6611359B2 (ja) 2019-11-27
KR20160125370A (ko) 2016-10-31
CA2937124A1 (en) 2015-07-23
WO2015109168A1 (en) 2015-07-23
HK1232126A1 (zh) 2018-01-05
US20160340701A1 (en) 2016-11-24
EP3094320A1 (en) 2016-11-23
PL3094320T3 (pl) 2023-12-04

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