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MX2016004564A - Cultivo celular metabolicamente optimizado. - Google Patents

Cultivo celular metabolicamente optimizado.

Info

Publication number
MX2016004564A
MX2016004564A MX2016004564A MX2016004564A MX2016004564A MX 2016004564 A MX2016004564 A MX 2016004564A MX 2016004564 A MX2016004564 A MX 2016004564A MX 2016004564 A MX2016004564 A MX 2016004564A MX 2016004564 A MX2016004564 A MX 2016004564A
Authority
MX
Mexico
Prior art keywords
cell culture
present
proteins
accordance
optimized cell
Prior art date
Application number
MX2016004564A
Other languages
English (en)
Other versions
MX376623B (es
Inventor
Amy Johnson
Shawn Lawrence
Ann Kim
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52813657&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016004564(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2016004564A publication Critical patent/MX2016004564A/es
Publication of MX376623B publication Critical patent/MX376623B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2511/00—Cells for large scale production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a un método mejorado, para la producción a gran escala de proteínas y/o polipéptidos en un cultivo celular. De conformidad con la presente invención, el método permite cultivar células que presentan una modificación metabólica. El uso de tal método o sistema permite la producción de altas concentraciones de la proteína o polipéptido de interés y reduce la acumulación de desechos metabólicos no deseados, tales como el lactato. Las proteínas y polipéptidos expresados de conformidad con la presente invención, se pueden utilizar ventajosamente en la preparación de composiciones farmacéuticas, inmunogénicas, u otras composiciones biológicas comerciales, tales como anticuerpos.
MX2016004564A 2013-10-11 2014-10-10 Cultivo celular metabolicamente optimizado. MX376623B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361889815P 2013-10-11 2013-10-11
PCT/US2014/059993 WO2015054554A1 (en) 2013-10-11 2014-10-10 Metabolically optimized cell culture

Publications (2)

Publication Number Publication Date
MX2016004564A true MX2016004564A (es) 2016-07-12
MX376623B MX376623B (es) 2025-03-07

Family

ID=52813657

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2020011334A MX384960B (es) 2013-10-11 2014-10-10 Cultivo celular metabolicamente optimizado
MX2016004564A MX376623B (es) 2013-10-11 2014-10-10 Cultivo celular metabolicamente optimizado.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2020011334A MX384960B (es) 2013-10-11 2014-10-10 Cultivo celular metabolicamente optimizado

Country Status (17)

Country Link
US (2) US20160244725A1 (es)
EP (3) EP3812453A1 (es)
JP (3) JP6663854B2 (es)
KR (4) KR102510238B1 (es)
CN (2) CN114717196A (es)
AU (2) AU2014331776B2 (es)
CA (1) CA2926049C (es)
DK (2) DK3055409T3 (es)
EA (2) EA201991976A1 (es)
ES (2) ES2678945T3 (es)
HU (2) HUE039551T2 (es)
IL (2) IL244511A0 (es)
MX (2) MX384960B (es)
PL (2) PL3351620T3 (es)
SG (2) SG10201800772TA (es)
WO (1) WO2015054554A1 (es)
ZA (1) ZA201601550B (es)

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US20160244726A1 (en) * 2013-10-11 2016-08-25 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
US11390663B2 (en) 2013-10-11 2022-07-19 Regeneron Pharmaceuticals, Inc. Metabolically optimized cell culture
JP7260011B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260020B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7260018B2 (ja) * 2017-03-31 2023-04-18 株式会社三洋物産 遊技機
JP7310947B2 (ja) * 2017-04-27 2023-07-19 株式会社三洋物産 遊技機
JP7260021B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
JP7260013B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
JP7272480B2 (ja) * 2017-04-27 2023-05-12 株式会社三洋物産 遊技機
JP7260009B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
JP7310952B2 (ja) * 2017-04-27 2023-07-19 株式会社三洋物産 遊技機
JP7260012B2 (ja) * 2017-04-27 2023-04-18 株式会社三洋物産 遊技機
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CN111344558A (zh) 2017-10-06 2020-06-26 龙沙有限公司 使用拉曼光谱法自动控制细胞培养
EA202090740A1 (ru) 2017-10-16 2020-07-07 Ридженерон Фармасьютикалз, Инк. Перфузионный биореактор и связанные с ним способы применения
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EP3870604B1 (en) 2018-10-26 2022-11-23 F. Hoffmann-La Roche AG Multispecific antibody screening method using recombinase mediated cassette exchange
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US6165741A (en) 1997-05-30 2000-12-26 The Trustees Of The University Of Pennsylvania Method for rapid detection of bacterial growth in cultures
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Also Published As

Publication number Publication date
IL262833A (en) 2018-12-31
EP3055409B1 (en) 2018-04-25
SG11201601681QA (en) 2016-04-28
EP3351620A1 (en) 2018-07-25
EP3351620B1 (en) 2020-09-02
PL3351620T3 (pl) 2021-03-08
HK1223122A1 (en) 2017-07-21
JP2016532457A (ja) 2016-10-20
MX2020011334A (es) 2021-07-30
KR20160062032A (ko) 2016-06-01
CN105637085A (zh) 2016-06-01
ES2833471T3 (es) 2021-06-15
HUE039551T2 (hu) 2019-01-28
KR102358807B1 (ko) 2022-02-08
EA033783B1 (ru) 2019-11-25
KR102510238B1 (ko) 2023-03-16
JP6663854B2 (ja) 2020-03-13
MX376623B (es) 2025-03-07
HUE051049T2 (hu) 2021-01-28
JP2020072760A (ja) 2020-05-14
SG10201800772TA (en) 2018-03-28
KR102610425B1 (ko) 2023-12-06
HK1253275A1 (en) 2019-06-14
IL244511A0 (en) 2016-04-21
AU2014331776A1 (en) 2016-03-24
KR20210046835A (ko) 2021-04-28
US20210087535A1 (en) 2021-03-25
JP7377243B2 (ja) 2023-11-09
CA2926049C (en) 2023-09-05
KR102243980B1 (ko) 2021-04-26
JP6967620B2 (ja) 2021-11-17
EA201690600A1 (ru) 2016-09-30
CN114717196A (zh) 2022-07-08
DK3055409T3 (en) 2018-07-30
EP3055409A4 (en) 2016-09-28
EA201991976A1 (ru) 2020-04-02
MX384960B (es) 2025-03-14
PL3055409T3 (pl) 2018-10-31
KR20230038324A (ko) 2023-03-17
ZA201601550B (en) 2023-05-31
ES2678945T3 (es) 2018-08-21
JP2022009453A (ja) 2022-01-14
WO2015054554A1 (en) 2015-04-16
DK3351620T3 (da) 2020-10-26
US20160244725A1 (en) 2016-08-25
KR20220020415A (ko) 2022-02-18
EP3812453A1 (en) 2021-04-28
AU2014331776B2 (en) 2020-10-22
EP3055409A1 (en) 2016-08-17
AU2021200193A1 (en) 2021-03-18
CA2926049A1 (en) 2015-04-16
AU2021200193B2 (en) 2023-08-24

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