MX2014000378A - Analisis rapdo y preciso de la sialilacion de proteinas. - Google Patents
Analisis rapdo y preciso de la sialilacion de proteinas.Info
- Publication number
- MX2014000378A MX2014000378A MX2014000378A MX2014000378A MX2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A MX 2014000378 A MX2014000378 A MX 2014000378A
- Authority
- MX
- Mexico
- Prior art keywords
- gluco
- sialylation
- protein
- galactose
- rapid
- Prior art date
Links
- 230000009450 sialylation Effects 0.000 title abstract 3
- 238000004458 analytical method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 229930182830 galactose Natural products 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000005936 beta-Galactosidase Human genes 0.000 abstract 2
- 108010005774 beta-Galactosidase Proteins 0.000 abstract 2
- 238000005571 anion exchange chromatography Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Emergency Medicine (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
Métodos y kits para el análisis de la sialilación de glucoproteínas. Las muestras de glucoproteína se incuban por separado en tres condiciones: con beta-galactosidasa, con beta-galactosidasa + alfa-sialidasa, y sin enzima. Tras el tratamiento enzimático, se determinan cuantitativamente mediante cromatografía de intercambio aniónico de alta resolución con detección amperométrica pulsada (HPAEC PAD) la galactosa total en la muestra, la galactosa no sialilada y la galactosa exógena del medio. La determinación de estos valores permite deducir el porcentaje de sialilación de la glucoproteína.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161507643P | 2011-07-14 | 2011-07-14 | |
| PCT/ES2012/070501 WO2013011178A1 (es) | 2011-07-14 | 2012-07-05 | Análisis rápido y preciso de la sialilación de proteínas |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014000378A true MX2014000378A (es) | 2014-03-31 |
| MX344407B MX344407B (es) | 2016-12-14 |
Family
ID=47557705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014000378A MX344407B (es) | 2011-07-14 | 2012-07-05 | Analisis rapdo y preciso de la sialilacion de proteinas. |
Country Status (22)
| Country | Link |
|---|---|
| US (1) | US9260743B2 (es) |
| EP (1) | EP2733218B1 (es) |
| JP (1) | JP2014525742A (es) |
| KR (1) | KR101864468B1 (es) |
| CN (1) | CN103635590A (es) |
| AR (1) | AR087175A1 (es) |
| AU (1) | AU2012285696B2 (es) |
| BR (1) | BR112013033883B1 (es) |
| CA (1) | CA2837981C (es) |
| CL (1) | CL2013003713A1 (es) |
| ES (1) | ES2675511T3 (es) |
| HU (1) | HUE038020T2 (es) |
| IL (1) | IL229828A (es) |
| MX (1) | MX344407B (es) |
| MY (1) | MY163078A (es) |
| PL (1) | PL2733218T3 (es) |
| PT (1) | PT2733218T (es) |
| RU (1) | RU2605900C2 (es) |
| SG (1) | SG10201500002XA (es) |
| TR (1) | TR201809082T4 (es) |
| UY (1) | UY34195A (es) |
| WO (1) | WO2013011178A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9260743B2 (en) * | 2011-07-14 | 2016-02-16 | Grifols. S.A. | Rapid and accurate analysis of protein sialylation |
| CN106324234B (zh) * | 2016-08-08 | 2018-05-22 | 上海睿康生物科技有限公司 | 修饰的n-乙酰神经氨酸醛缩酶及其制备方法和应用 |
| CN107045019B (zh) * | 2016-09-30 | 2019-08-16 | 中国医学科学院输血研究所 | 一种IVIG中IgG Fab片段和Fc片段唾液酸含量的测定方法 |
| CN110346500B (zh) * | 2018-04-04 | 2021-11-30 | 青岛大学附属医院 | 一种基于微波酸水解的阴离子交换色谱-脉冲安培法检测血清中单糖含量的检测方法 |
| CN109329713B (zh) * | 2018-12-01 | 2022-04-22 | 西华大学 | 一种降解牦牛肉中n-羟乙酰神经唾液酸的方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1503788B1 (en) * | 2002-04-25 | 2011-06-29 | Shire Human Genetic Therapies, Inc. | Treatment of alpha-galactosidase a deficiency |
| DE02020425T1 (de) * | 2002-09-11 | 2004-07-15 | Fresenius Kabi Deutschland Gmbh | Hasylierte Polypeptide, besonders hasyliertes Erythropoietin |
| US8039208B2 (en) | 2005-04-26 | 2011-10-18 | National Institute For Bioprocessing Research And Training Limited (Nibrt) | Automated strategy for identifying physiological glycosylation markers(s) |
| ATE447715T1 (de) * | 2005-04-26 | 2009-11-15 | Nat Inst For Bioproc Res And T | Automatische glykofingerabdruck-strategie |
| ES2517603T3 (es) * | 2008-04-07 | 2014-11-03 | Bayer Healthcare, Llc | Procedimientos de producción recombinante de glucoproteínas |
| US20110086362A1 (en) * | 2009-10-09 | 2011-04-14 | Massachusetts Institute Of Technology | High-Throughput Method for Quantifying Sialylation of Glycoproteins |
| US9260743B2 (en) * | 2011-07-14 | 2016-02-16 | Grifols. S.A. | Rapid and accurate analysis of protein sialylation |
-
2012
- 2012-07-05 US US14/232,783 patent/US9260743B2/en active Active
- 2012-07-05 RU RU2013154278/10A patent/RU2605900C2/ru active
- 2012-07-05 MX MX2014000378A patent/MX344407B/es active IP Right Grant
- 2012-07-05 TR TR2018/09082T patent/TR201809082T4/tr unknown
- 2012-07-05 PL PL12815322T patent/PL2733218T3/pl unknown
- 2012-07-05 PT PT128153228T patent/PT2733218T/pt unknown
- 2012-07-05 AU AU2012285696A patent/AU2012285696B2/en active Active
- 2012-07-05 JP JP2014519588A patent/JP2014525742A/ja active Pending
- 2012-07-05 CN CN201280033128.7A patent/CN103635590A/zh active Pending
- 2012-07-05 MY MYPI2013702595A patent/MY163078A/en unknown
- 2012-07-05 WO PCT/ES2012/070501 patent/WO2013011178A1/es not_active Ceased
- 2012-07-05 SG SG10201500002XA patent/SG10201500002XA/en unknown
- 2012-07-05 BR BR112013033883-0A patent/BR112013033883B1/pt not_active IP Right Cessation
- 2012-07-05 ES ES12815322.8T patent/ES2675511T3/es active Active
- 2012-07-05 CA CA2837981A patent/CA2837981C/en active Active
- 2012-07-05 EP EP12815322.8A patent/EP2733218B1/en active Active
- 2012-07-05 KR KR1020147003822A patent/KR101864468B1/ko not_active Expired - Fee Related
- 2012-07-05 HU HUE12815322A patent/HUE038020T2/hu unknown
- 2012-07-12 UY UY0001034195A patent/UY34195A/es active IP Right Grant
- 2012-07-13 AR ARP120102538A patent/AR087175A1/es active IP Right Grant
-
2013
- 2013-12-05 IL IL229828A patent/IL229828A/en active IP Right Grant
- 2013-12-24 CL CL2013003713A patent/CL2013003713A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| KR20140060289A (ko) | 2014-05-19 |
| EP2733218B1 (en) | 2018-05-30 |
| EP2733218A1 (en) | 2014-05-21 |
| IL229828A (en) | 2016-09-29 |
| US20140162299A1 (en) | 2014-06-12 |
| CA2837981A1 (en) | 2013-01-24 |
| MX344407B (es) | 2016-12-14 |
| RU2013154278A (ru) | 2015-06-20 |
| AR087175A1 (es) | 2014-02-26 |
| WO2013011178A1 (es) | 2013-01-24 |
| AU2012285696A1 (en) | 2013-05-09 |
| PL2733218T3 (pl) | 2018-08-31 |
| CN103635590A (zh) | 2014-03-12 |
| RU2605900C2 (ru) | 2016-12-27 |
| NZ618889A (en) | 2015-08-28 |
| SG10201500002XA (en) | 2015-02-27 |
| BR112013033883A2 (pt) | 2017-02-14 |
| BR112013033883B1 (pt) | 2020-11-03 |
| US9260743B2 (en) | 2016-02-16 |
| JP2014525742A (ja) | 2014-10-02 |
| UY34195A (es) | 2013-02-28 |
| MY163078A (en) | 2017-08-15 |
| EP2733218A4 (en) | 2015-03-25 |
| KR101864468B1 (ko) | 2018-06-04 |
| TR201809082T4 (tr) | 2018-07-23 |
| PT2733218T (pt) | 2018-07-04 |
| CL2013003713A1 (es) | 2014-08-08 |
| ES2675511T3 (es) | 2018-07-11 |
| AU2012285696B2 (en) | 2015-12-10 |
| HUE038020T2 (hu) | 2018-09-28 |
| CA2837981C (en) | 2020-08-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |