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MX2014000078A - Detection and quantification of lactic acid producing bacteria in food products. - Google Patents

Detection and quantification of lactic acid producing bacteria in food products.

Info

Publication number
MX2014000078A
MX2014000078A MX2014000078A MX2014000078A MX2014000078A MX 2014000078 A MX2014000078 A MX 2014000078A MX 2014000078 A MX2014000078 A MX 2014000078A MX 2014000078 A MX2014000078 A MX 2014000078A MX 2014000078 A MX2014000078 A MX 2014000078A
Authority
MX
Mexico
Prior art keywords
lactic acid
quantification
detection
food products
producing bacteria
Prior art date
Application number
MX2014000078A
Other languages
Spanish (es)
Inventor
Yuewei Hu
Mueve C Murphy
Kelly Stevens
Original Assignee
Gen Mills Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Mills Inc filed Critical Gen Mills Inc
Publication of MX2014000078A publication Critical patent/MX2014000078A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods and kits for rapidly detecting and/or quantifying lactic acid bacteria using real time PCR including a first primer, a second primer, and a probe. Each of the primers and the probe are complementary to separate regions of the 16S rRNA gene of lactic acid bacteria. The methods and kits may be used for the detection and/or quantification of lactic acid bacteria in food such as yogurt, to support product labeling, and in salsa.
MX2014000078A 2011-06-27 2012-06-25 Detection and quantification of lactic acid producing bacteria in food products. MX2014000078A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161501470P 2011-06-27 2011-06-27
PCT/US2012/043958 WO2013003262A1 (en) 2011-06-27 2012-06-25 Detection and quantification of lactic acid producing bacteria in food products

Publications (1)

Publication Number Publication Date
MX2014000078A true MX2014000078A (en) 2014-05-01

Family

ID=47424497

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014000078A MX2014000078A (en) 2011-06-27 2012-06-25 Detection and quantification of lactic acid producing bacteria in food products.

Country Status (7)

Country Link
EP (1) EP2723903A4 (en)
KR (1) KR20140057246A (en)
CN (1) CN103930564A (en)
AU (1) AU2012275650A1 (en)
CA (1) CA2840364A1 (en)
MX (1) MX2014000078A (en)
WO (1) WO2013003262A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT201600097963A1 (en) * 2016-09-29 2018-03-29 Univ Degli Studi Di Bari Aldo Moro Molecular method aimed at authenticating traditional / typical bread obtained with natural yeast, primers used in the said method and kit containing said primers.
CN107937581B (en) * 2017-12-28 2021-09-17 内蒙古农业大学 Amplification primer pair for lactobacillus sequencing, lactobacillus species identification method and application
WO2019200134A2 (en) * 2018-04-13 2019-10-17 National Agricultural Genotyping Center Universal lactic acid bacteria quantification kits, methods, compositions and apparatuses therefor
CN113817810A (en) * 2021-08-09 2021-12-21 宁波大学 A novel qPCR method for detecting lactic acid bacteria in fermented milk

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
JP3164213B2 (en) * 1998-08-20 2001-05-08 日本電気株式会社 Method of forming embedded metal wiring
JP2005229839A (en) * 2004-02-17 2005-09-02 Sapporo Breweries Ltd Method for detecting and identifying lactic acid bacterium
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
JP4813215B2 (en) * 2005-03-08 2011-11-09 三星電子株式会社 Probe set design method, probe set designed thereby, microarray including the probe set, computer-readable recording medium recording a program enabling a computer to execute the method, and target sequence using the probe set Identification method
JP2006333801A (en) * 2005-06-03 2006-12-14 Obihiro Univ Of Agriculture & Veterinary Medicine Method for detecting canine abortion and leptospira and primer set used therefor
US20110177960A1 (en) * 2006-03-10 2011-07-21 Ellen Murphy Microarray for monitoring gene expression in multiple strains of Streptococcus pneumoniae
FR2908785B1 (en) * 2006-11-17 2012-09-28 Air Liquide MICROORGANISM DETECTION METHODS BY PCR

Also Published As

Publication number Publication date
EP2723903A4 (en) 2015-02-18
KR20140057246A (en) 2014-05-12
AU2012275650A1 (en) 2014-01-16
CN103930564A (en) 2014-07-16
WO2013003262A1 (en) 2013-01-03
EP2723903A1 (en) 2014-04-30
WO2013003262A9 (en) 2014-02-13
CA2840364A1 (en) 2013-01-03

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