MX2013007510A - Metodo de congelacion de celulas. - Google Patents
Metodo de congelacion de celulas.Info
- Publication number
- MX2013007510A MX2013007510A MX2013007510A MX2013007510A MX2013007510A MX 2013007510 A MX2013007510 A MX 2013007510A MX 2013007510 A MX2013007510 A MX 2013007510A MX 2013007510 A MX2013007510 A MX 2013007510A MX 2013007510 A MX2013007510 A MX 2013007510A
- Authority
- MX
- Mexico
- Prior art keywords
- freezing
- cells
- cryoprotectant
- obtaining
- thawing
- Prior art date
Links
- 230000008014 freezing Effects 0.000 title abstract 4
- 238000007710 freezing Methods 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 239000002577 cryoprotective agent Substances 0.000 abstract 4
- 239000012895 dilution Substances 0.000 abstract 2
- 238000010790 dilution Methods 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 238000010257 thawing Methods 0.000 abstract 2
- 230000007717 exclusion Effects 0.000 abstract 1
- 239000012737 fresh medium Substances 0.000 abstract 1
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 230000003000 nontoxic effect Effects 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/16—Physical preservation processes
- A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
- A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
- A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
- A01N1/147—Carriers for immersion in cryogenic fluid for slow freezing or vitrification
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
La invención da a conocer un vial de congelación de células de múltiples capas y un método de obtención del mismo. El método de obtención es un procedimiento de congelación y descongelación de células que evita la necesidad de incorporar nuevo medio recién preparado a las células descongeladas para la dilución del agente crioprotector. Esto se logra mediante la congelación previa de una capa extra de medio de cultivo además de la disolución de células congeladas que contiene dicho agente crioprotector. En el momento de la descongelación, el medio de cultivo y la disolución de células se mezclan entre sí, cambiando la concentración de dicho agente crioprotector a una dilución no tóxica y logrando una exclusión eficaz de dicho agente crioprotector de las células vivas.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10016179.3A EP2471359B1 (en) | 2010-12-30 | 2010-12-30 | Method of freezing cells |
| PCT/EP2011/006579 WO2012089337A1 (en) | 2010-12-30 | 2011-12-15 | Method of freezing cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2013007510A true MX2013007510A (es) | 2013-08-01 |
| MX346168B MX346168B (es) | 2017-02-27 |
Family
ID=44041508
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013007510A MX346168B (es) | 2010-12-30 | 2011-12-15 | Metodo de congelacion de celulas. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9642354B2 (es) |
| EP (1) | EP2471359B1 (es) |
| JP (1) | JP2014502610A (es) |
| CN (1) | CN103269580B (es) |
| AU (1) | AU2011351883B2 (es) |
| BR (1) | BR112013016341A2 (es) |
| CA (1) | CA2821998A1 (es) |
| ES (1) | ES2576733T3 (es) |
| IL (1) | IL227121A (es) |
| MX (1) | MX346168B (es) |
| WO (1) | WO2012089337A1 (es) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3035798T3 (en) * | 2013-08-20 | 2018-11-19 | Univ Yeditepe | CELL CRYO-CONSERVATION MEDIUM ADDED TO DRILL |
| US9615570B2 (en) | 2013-11-20 | 2017-04-11 | Cellulis, S.L. | Method of freezing cells |
| US11470841B2 (en) | 2016-06-15 | 2022-10-18 | Nissan Chemical Corporation | Cryopreservation vessel |
| TW201923065A (zh) | 2017-09-26 | 2019-06-16 | 日商日產化學股份有限公司 | 具有微小容積之細胞培養容器 |
| CN108094406A (zh) * | 2017-12-28 | 2018-06-01 | 重庆斯德姆生物技术有限公司 | 一种细胞冷冻、快速复苏与培养的方法 |
| CN108949565B (zh) * | 2018-09-26 | 2024-09-06 | 中国科学技术大学 | 用于红细胞负载冻干保护剂的装置及方法 |
| US11116206B2 (en) | 2018-10-01 | 2021-09-14 | Cook Medical Technologies Llc | Cryocontainer |
| CN109566604B (zh) * | 2019-01-31 | 2021-03-19 | 北京华龛生物科技有限公司 | 一种三维微载体原位冷冻保存细胞的方法 |
| CN118541029A (zh) * | 2021-11-15 | 2024-08-23 | 组织测试技术有限公司 | 天然和生物工程改造组织的保存以及储存和运输方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2631706A1 (fr) | 1988-05-19 | 1989-11-24 | Ire Celltarg Sa | Test de diagnostic de maladie virale et procede de congelation de cellules utiles pour ledit test |
| JPH03232441A (ja) * | 1990-02-09 | 1991-10-16 | Zenkoku Nogyo Kyodo Kumiai Rengokai | 受胎率を上げた凍結受精卵およびその調製法 |
| JP3672201B2 (ja) * | 1991-12-26 | 2005-07-20 | 雪印乳業株式会社 | 哺乳動物胚移植用ストロー及びその製造法 |
| JP2582327B2 (ja) * | 1992-10-29 | 1997-02-19 | 宮崎県 | 哺乳動物胚の凍結保護物質の簡易除去法 |
| DE4438232A1 (de) * | 1994-10-26 | 1996-05-02 | Guenter Prof Dr Fuhr | Kryokonservierung und Tieftemperaturbearbeitung von biologischen Zellen |
| JP4942896B2 (ja) | 2000-04-17 | 2012-05-30 | オーガン リカヴァリー システムズ インコーポレイテッド | 新規な凍結試料の加温方法 |
| US6472206B1 (en) | 2000-04-28 | 2002-10-29 | Interthyr Corporation | In situ growth, freezing and testing of cultured cells |
| JP4849725B2 (ja) | 2001-03-05 | 2012-01-11 | 住友ベークライト株式会社 | 細胞付培養基板及びその製造方法 |
| US20050106554A1 (en) * | 2003-11-19 | 2005-05-19 | Palecek Sean P. | Cryopreservation of pluripotent stem cells |
| DE102004061947A1 (de) * | 2004-12-22 | 2006-07-06 | Ccs Cell Culture Service Gmbh | Verfahren zum Einfrieren und Lagern von Zellen |
| US20090170059A1 (en) * | 2005-11-14 | 2009-07-02 | Hans Klingemann | Methods for Preparing Cord Matrix Stem Cells (CMSC) for Long Term Storage and for Preparing a Segment of umbilical cord for cryopreservation |
| EP1869976A1 (de) | 2006-06-21 | 2007-12-26 | SiRion GmbH | Verfahren zum Einfrieren und Lagern von Zellen |
| JP5100078B2 (ja) * | 2006-10-05 | 2012-12-19 | 株式会社セルバンク | 組織由来細胞の凍結保存方法 |
| US9169460B2 (en) * | 2009-05-19 | 2015-10-27 | Lifeglobal Group Llc | Flooding dish and method for changing media in the dish in the preparation of mammalian specimen culture and for cryo-preservation, freezing, vitrification and the thawing and warming of such specimens |
-
2010
- 2010-12-30 EP EP10016179.3A patent/EP2471359B1/en not_active Not-in-force
- 2010-12-30 ES ES10016179.3T patent/ES2576733T3/es active Active
-
2011
- 2011-12-15 JP JP2013546613A patent/JP2014502610A/ja active Pending
- 2011-12-15 MX MX2013007510A patent/MX346168B/es active IP Right Grant
- 2011-12-15 CN CN201180062741.7A patent/CN103269580B/zh not_active Expired - Fee Related
- 2011-12-15 BR BR112013016341A patent/BR112013016341A2/pt not_active Application Discontinuation
- 2011-12-15 US US13/997,408 patent/US9642354B2/en not_active Expired - Fee Related
- 2011-12-15 CA CA2821998A patent/CA2821998A1/en not_active Abandoned
- 2011-12-15 WO PCT/EP2011/006579 patent/WO2012089337A1/en not_active Ceased
- 2011-12-15 AU AU2011351883A patent/AU2011351883B2/en not_active Ceased
-
2013
- 2013-06-20 IL IL227121A patent/IL227121A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2821998A1 (en) | 2012-07-05 |
| IL227121A (en) | 2016-02-29 |
| MX346168B (es) | 2017-02-27 |
| ES2576733T3 (es) | 2016-07-11 |
| EP2471359A1 (en) | 2012-07-04 |
| AU2011351883A1 (en) | 2013-07-11 |
| CN103269580B (zh) | 2015-10-14 |
| EP2471359B1 (en) | 2016-03-30 |
| US20140004500A1 (en) | 2014-01-02 |
| JP2014502610A (ja) | 2014-02-03 |
| CN103269580A (zh) | 2013-08-28 |
| WO2012089337A1 (en) | 2012-07-05 |
| US9642354B2 (en) | 2017-05-09 |
| AU2011351883B2 (en) | 2015-07-02 |
| BR112013016341A2 (pt) | 2018-06-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2013007510A (es) | Metodo de congelacion de celulas. | |
| WO2011083472A3 (en) | Method for preserving cancellous bone samples and preserved cancellous bone tissue | |
| MX391259B (es) | Composiciones y métodos para reducir la formación de cristales de hielo. | |
| MX2014011005A (es) | Sistema y sustancias para la crioconservacion de celulas viables. | |
| CL2017000998A1 (es) | Composición que comprende un agente de control biológico de la especie bacillus subtilis y un fungicida del grupo de inhibidores de la mitosis y la división celular, tal como fenamidona. (divisional solicitud 3272-2014) | |
| RU2015154110A (ru) | Содержащий трегалозу и декстран раствор для трансплантации клеток млекопитающих | |
| AR093662A1 (es) | Agentes crioprotectores, composiciones crioprotectoras y criopreservadas, sus usos y metodos de criopreservacion | |
| EA201300323A1 (ru) | Материалы и способы улучшения функции желудочно-кишечного тракта | |
| MX2011008373A (es) | Procedimiento para la criopreservacion de celulas, construcciones celulares artificiales o ensamblajes de tejidos complejos tridimensionales. | |
| AR082586A1 (es) | Productos dulces no perecederos | |
| EA202190722A1 (ru) | Клинические композиции | |
| CY1120841T1 (el) | Συνθεση και μεθοδος για τη διατηρηση, τη μεταφορα και την αποθηκευση ζωντανων βιολογικων υλικων | |
| MX2012015063A (es) | Metodos y composiciones para mejorar la movilizacion de celulas madre. | |
| GT201200229A (es) | Combinaciones de principios activos acaricidas y/o insecticidas | |
| MX2017007803A (es) | Conservación y transporte de una muestra biológica ex vivo que comprende aplicación por ultrasonido. | |
| EA201791382A1 (ru) | Стабильная лекарственная форма замороженного вируса простого герпеса | |
| CR20150310A (es) | Solución para preservar conductos vasculares | |
| MX2017000231A (es) | Ajuste del ph para mejorar la recuperacion por descongelamiento de bancos de celulas. | |
| MX373950B (es) | USO DE UN INHIBIDOR TEMPORAL DE UN GEN p53 PARA LA PROFILAXIS DE LESIÓN POR ISQUEMIA-REPERFUSIÓN. | |
| AR088166A1 (es) | Metodo para la preservacion de tejido vivo | |
| JP2016512693A5 (es) | ||
| BR112012027084A2 (pt) | célula solar orgânica de heterojunção em um espaço incluindo uma camada eletricamente ativa e tendo secregação verical. | |
| WO2014045202A3 (en) | Cell preparation method | |
| CL2020000069A1 (es) | Mejora de las características del suelo con compuestos lactobionatos. | |
| CN103891710B (zh) | 一种Oscheius tipulae线虫冻存液及其制备方法及该冻存液的应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |