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MX2012013037A - Composiciones de endorribonucleasa y metodos de uso de las mismas. - Google Patents

Composiciones de endorribonucleasa y metodos de uso de las mismas.

Info

Publication number
MX2012013037A
MX2012013037A MX2012013037A MX2012013037A MX2012013037A MX 2012013037 A MX2012013037 A MX 2012013037A MX 2012013037 A MX2012013037 A MX 2012013037A MX 2012013037 A MX2012013037 A MX 2012013037A MX 2012013037 A MX2012013037 A MX 2012013037A
Authority
MX
Mexico
Prior art keywords
methods
present disclosure
endoribonuclease
compositions
variant csy4
Prior art date
Application number
MX2012013037A
Other languages
English (en)
Inventor
Rachel E Haurwitz
Jennifer A Doudna
Blake Wiedenheft
Martin Jinek
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California filed Critical Univ California
Publication of MX2012013037A publication Critical patent/MX2012013037A/es

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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K1/14—Extraction; Separation; Purification
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

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  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención se refiere a variantes de endorribonucleasas Csy4, a ácidos nucleicos que codifican las variantes de endorribonucleasas Csy4 y las células huésped genéticamente modificadas con los ácidos nucleicos. La invención se refiere también a las diversas aplicaciones en las cuales encuentran un uso las variantes de endorribonucleasas Csy4. La presente invención proporciona también métodos de detección de una secuencia específica en un polirribonucleótido objetivo, y métodos para regular la producción de un ARN objetivo en una célula eucariota.
MX2012013037A 2010-05-10 2011-05-09 Composiciones de endorribonucleasa y metodos de uso de las mismas. MX2012013037A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33316310P 2010-05-10 2010-05-10
US36562710P 2010-07-19 2010-07-19
US41328710P 2010-11-12 2010-11-12
PCT/US2011/035775 WO2011143124A2 (en) 2010-05-10 2011-05-09 Endoribonuclease compositions and methods of use thereof

Publications (1)

Publication Number Publication Date
MX2012013037A true MX2012013037A (es) 2013-07-29

Family

ID=44914921

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012013037A MX2012013037A (es) 2010-05-10 2011-05-09 Composiciones de endorribonucleasa y metodos de uso de las mismas.

Country Status (14)

Country Link
US (3) US9115348B2 (es)
EP (2) EP3078753B1 (es)
JP (1) JP5926242B2 (es)
KR (1) KR101867359B1 (es)
CN (1) CN103038338B (es)
AU (1) AU2011253222B2 (es)
BR (1) BR112012028805A2 (es)
CA (1) CA2798703A1 (es)
DK (1) DK2569425T3 (es)
EA (1) EA024121B9 (es)
ES (1) ES2590343T3 (es)
MX (1) MX2012013037A (es)
SG (1) SG185481A1 (es)
WO (1) WO2011143124A2 (es)

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