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MX2012009357A - Enzimas celulasas optimizadas. - Google Patents

Enzimas celulasas optimizadas.

Info

Publication number
MX2012009357A
MX2012009357A MX2012009357A MX2012009357A MX2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A MX 2012009357 A MX2012009357 A MX 2012009357A
Authority
MX
Mexico
Prior art keywords
enzymes
disclosed
cellulase enzymes
cellulose
processing
Prior art date
Application number
MX2012009357A
Other languages
English (en)
Other versions
MX344963B (es
Inventor
Ulrich Kettling
Andre Koltermann
Christoph Reisinger
Brueck Thomas
Jochen Gerlach
Isabel Unterstrasser
Lutz Roecher
Markus Rarbach
Joerg Claren
Andreas Kohl
Jan Carsten Pieck
Dominik Schlosser
Original Assignee
Sued Chemie Ip Gmbh & Co Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sued Chemie Ip Gmbh & Co Kg filed Critical Sued Chemie Ip Gmbh & Co Kg
Publication of MX2012009357A publication Critical patent/MX2012009357A/es
Publication of MX344963B publication Critical patent/MX344963B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La invención desvela enzimas celulasa con propiedades optimizadas para procesamiento de sustratos que contienen celulosa y lignocelulosa. En particular, se desvelan enzimas celobiohidrolasa con características preferidas. La presente invención proporciona variantes de fusión, inserción, deleción y/o sustitución de tales enzimas. Las variantes enzimáticas tienen termoestabilidad, estabilidad proteolítica, actividad específica y/o estabilidad potenciadas a pH extremo. Se desvelan moléculas de ácido nucleico que codifican dichas enzimas, una composición que comprende dichas enzimas, un procedimiento para preparación y el uso para procesamiento de celulosa y/o para la producción de biocarburantes.
MX2012009357A 2010-02-11 2011-02-11 Enzimas celulasas optimizadas. MX344963B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10153355.2A EP2357227B1 (en) 2010-02-11 2010-02-11 Optimized cellulase enzymes
PCT/EP2011/052023 WO2011098551A2 (en) 2010-02-11 2011-02-11 Optimized cellulase enzymes

Publications (2)

Publication Number Publication Date
MX2012009357A true MX2012009357A (es) 2012-12-17
MX344963B MX344963B (es) 2017-01-11

Family

ID=42027636

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012009357A MX344963B (es) 2010-02-11 2011-02-11 Enzimas celulasas optimizadas.

Country Status (14)

Country Link
US (1) US9458443B2 (es)
EP (2) EP2357227B1 (es)
CN (1) CN102844430A (es)
AU (1) AU2011214298B2 (es)
BR (1) BR112012020277A2 (es)
CA (1) CA2789423C (es)
DK (2) DK2357227T3 (es)
ES (2) ES2549328T3 (es)
HR (2) HRP20181587T1 (es)
MX (1) MX344963B (es)
PL (2) PL2357227T3 (es)
RS (1) RS57695B1 (es)
SI (2) SI2357227T1 (es)
WO (1) WO2011098551A2 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012028206A2 (pt) 2010-06-01 2019-09-24 California Inst Of Techn enzimas de celobioidrolase quiméricas funcionais, estáveis, classe i.
EP2625269A2 (en) 2010-10-06 2013-08-14 BP Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
EP2691519A1 (en) * 2011-03-31 2014-02-05 Novozymes, Inc. Cellulose binding domain variants and polynucleotides encoding same
CA2856083A1 (en) * 2011-11-15 2013-05-23 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2013091577A1 (en) * 2011-12-22 2013-06-27 Novozymes, Inc. Hybrid polypeptides having cellobiohydrolase activity and polynucleotides encoding same
MX2015007235A (es) * 2012-12-12 2015-10-29 Danisco Us Inc Variantes de celobiohidrolasas.
CN104870644A (zh) 2012-12-12 2015-08-26 丹尼斯科美国公司 纤维二糖水解酶的变体
CN110628749A (zh) * 2013-03-08 2019-12-31 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
US11390898B2 (en) 2014-09-05 2022-07-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
WO2016040265A1 (en) 2014-09-08 2016-03-17 Novozymes A/S Cellobiohydrolase variants and polynucleotides encoding same
JP6378622B2 (ja) 2014-12-04 2018-08-22 本田技研工業株式会社 高活性セロビオヒドロラーゼ
FI3739045T3 (fi) 2015-02-24 2024-11-13 Novozymes As Sellobiohydrolaasivariantteja ja niitä koodittavia polynukleotideja
WO2017177289A1 (en) 2016-04-15 2017-10-19 Vtt International Oy Process for conversion of biomass into fermentable sugars with integrated enzyme
BR112018075932A2 (pt) * 2016-06-15 2019-10-01 Codexis Inc variante de beta-glicosidase de ocorrência não natural, polinucleotídeo recombinante, vetor, célula hospedeira, métodos para produção de pelo menos uma variante de beta-glicosidade de ocorrência não natural, para conversão de um substrato aceptor de grupo glicosila em um produto beta-glicosilado e para transglicosilação de um substrato, e, composição.
CN109337888B (zh) * 2018-11-12 2021-08-17 南开大学 纤维二糖水解酶突变体及其生产方法和应用
CN110373402B (zh) * 2019-08-13 2021-07-20 山东农业大学 一种提升耐热纤维素酶酶活力和热稳定性的方法
CN113186175B (zh) * 2021-06-04 2023-08-04 翌圣生物科技(上海)股份有限公司 突变型Taq DNA聚合酶、编码DNA序列、重组载体、重组表达细胞及其应用
CN114426961B (zh) * 2022-03-10 2024-01-26 安徽大学 一种β-葡萄糖苷酶突变体、其编码基因、表达菌株及其应用
CN117802072B (zh) * 2024-02-29 2024-05-24 北京挑战生物技术有限公司 木聚糖酶突变体及其应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
US8637293B2 (en) 1999-07-13 2014-01-28 Alliance For Sustainable Energy, Llc Cellobiohydrolase I enzymes
US7375197B2 (en) * 2002-01-14 2008-05-20 Midwest Research Institute Cellobiohydrolase I gene and improved variants
EP1200574A4 (en) 1999-07-13 2004-03-17 Midwest Research Inst REDUCED GLYCOSYLATION VARIANTS OF CELLOBIOHYDROLASE: CBHIN45A, CBHIN270A AND CBHIN384A
DK1421224T3 (da) 2001-06-26 2013-01-21 Novozymes As Polypeptider med cellobiohydrolase I aktivitet og polynukleotider der koder for samme
AU2003263842A1 (en) 2002-08-16 2004-03-03 Genencor International, Inc. Novel variant hyprocrea jecorina cbh1 cellulases
AU2003219956A1 (en) * 2003-02-27 2004-09-28 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
EP2298876A1 (en) 2003-03-21 2011-03-23 Genencor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
WO2005001065A2 (en) 2003-04-01 2005-01-06 Genencor International, Inc. Variant humicola grisea cbh1.1
EP2377931B1 (en) 2003-08-25 2013-05-08 Novozymes Inc. Variants of glycoside hydrolases
PT1874927E (pt) * 2005-04-29 2014-04-23 Ab Enzymes Oy Celulases melhoradas
CN1757710A (zh) 2005-07-15 2006-04-12 山东农业大学 表达cbh1基因的酵母工程菌株及其构建方法
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
US8017373B2 (en) * 2006-08-31 2011-09-13 Iogen Energy Corporation Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks
EP2260105B1 (en) 2008-02-29 2016-08-17 The Trustees Of The University Of Pennsylvania Production and use of plant degrading materials
WO2009139839A1 (en) * 2008-05-11 2009-11-19 Mascoma Corporation Construction of protrophic/celluloytic yeast strains expressing tethered and secreted cellulases
CA2724076C (en) * 2008-05-11 2018-11-27 Universiteit Stellenbosch Heterologous expression of fungal cellobiohydrolases in yeast
US8470592B2 (en) * 2008-07-07 2013-06-25 Mascoma Corporation Isolation and characterization of Schizochytrium aggregatum cellobiohydrolase I (Cbh 1)
DK2421965T3 (en) * 2009-04-24 2016-09-05 Dsm Ip Assets Bv Carbohydratdegraderende polypeptide and uses thereof

Also Published As

Publication number Publication date
WO2011098551A3 (en) 2012-05-31
EP2534245B1 (en) 2018-07-04
HRP20151043T1 (hr) 2015-11-06
CA2789423A1 (en) 2011-08-18
DK2534245T3 (en) 2018-10-01
SI2357227T1 (sl) 2015-11-30
EP2357227B1 (en) 2015-07-15
PL2534245T3 (pl) 2019-02-28
RS57695B1 (sr) 2018-11-30
EP2357227A1 (en) 2011-08-17
AU2011214298A1 (en) 2012-09-06
DK2357227T3 (en) 2015-09-14
US9458443B2 (en) 2016-10-04
WO2011098551A2 (en) 2011-08-18
US20130123115A1 (en) 2013-05-16
MX344963B (es) 2017-01-11
SI2534245T1 (sl) 2019-01-31
CA2789423C (en) 2017-12-12
ES2689939T3 (es) 2018-11-16
PL2357227T3 (pl) 2015-12-31
BR112012020277A2 (pt) 2015-09-15
EP2534245A2 (en) 2012-12-19
ES2549328T3 (es) 2015-10-27
AU2011214298B2 (en) 2014-11-06
CN102844430A (zh) 2012-12-26
HRP20181587T1 (hr) 2018-12-14

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